ANÁLISE ULTRAESTRUTURAL E NANOMECÂNICA DO VÍRUS SARS-CoV-2
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFMA |
Texto Completo: | https://tedebc.ufma.br/jspui/handle/tede/tede/4514 |
Resumo: | The ongoing outbreak of the severe acute respiratory syndrome coronavirus2 (SARSCoV2) started in later 2019 and spread across the world, infecting millions of people with over 5 million deaths worldwide. To fight back the virus is necessary to understand how its main structures function, especially those responsible for the virus infectivity pathogenicity. Understanding from the physical point of view the structure and properties of SARSCoV2 is extremely impor tant to find vulnerable points that can elucidate the mechanisms of drug targeting, for example. Determining the ultrastructure and nanomechanical properties of SARSCoV2 can clasifiy its mechanical properties and shed light on new treatment routes. This study has Atomic Force Microscopy as the main tool for the investigation of the ultrastructure and nanomechanical pro perties of the SARSCoV2 inactivated virion. Here, using the most advanced Atomic Force Microscopy techniques, SARSCoV2 viral particles were analyzed, with an especial focus on their ultrastructure, adsorption conformation and nanomechanical behavior. The results uncove red aspects of the organization and the spatial distribution of the proteins on the surface of the viral particles. It was also shown the compliant behavior of the membrane and ability to reco ver from mechanical injuries. At least three layers composing the membrane and their thickness were measured. This study provides new insight into the ultrastructure of SARSCoV2 particles at the nanoscale offering new prospects that could be employed for mapping viral surface. |
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ALENCAR, Luciana Magalhães Rebelohttp://lattes.cnpq.br/1109849519017980SANTOS-OLIVEIRA, Ralphhttp://lattes.cnpq.br/2376670019296363ALENCAR, Luciana Magalhães Rebelohttp://lattes.cnpq.br/1109849519017980SANTOSOLIVEIRA, Ralphhttp://lattes.cnpq.br/2376670019296363MENEZES, Alan Silva dehttp://lattes.cnpq.br/0383834501086115http://lattes.cnpq.br/9956397727510664LIMA, Ruana Cardoso2023-02-03T13:37:02Z2022-02-21LIMA, Ruana Cardoso. Análise ultraestrutural e nanomecânica do vírus SARS-CoV-2. 2022. 106 f. Dissertação( Programa de Pós-graduação em Física/CCET) - Universidade Federal do Maranhão, São Luís,2022.https://tedebc.ufma.br/jspui/handle/tede/tede/4514The ongoing outbreak of the severe acute respiratory syndrome coronavirus2 (SARSCoV2) started in later 2019 and spread across the world, infecting millions of people with over 5 million deaths worldwide. To fight back the virus is necessary to understand how its main structures function, especially those responsible for the virus infectivity pathogenicity. Understanding from the physical point of view the structure and properties of SARSCoV2 is extremely impor tant to find vulnerable points that can elucidate the mechanisms of drug targeting, for example. Determining the ultrastructure and nanomechanical properties of SARSCoV2 can clasifiy its mechanical properties and shed light on new treatment routes. This study has Atomic Force Microscopy as the main tool for the investigation of the ultrastructure and nanomechanical pro perties of the SARSCoV2 inactivated virion. Here, using the most advanced Atomic Force Microscopy techniques, SARSCoV2 viral particles were analyzed, with an especial focus on their ultrastructure, adsorption conformation and nanomechanical behavior. The results uncove red aspects of the organization and the spatial distribution of the proteins on the surface of the viral particles. It was also shown the compliant behavior of the membrane and ability to reco ver from mechanical injuries. At least three layers composing the membrane and their thickness were measured. This study provides new insight into the ultrastructure of SARSCoV2 particles at the nanoscale offering new prospects that could be employed for mapping viral surface.A eclosão da síndrome respiratória aguda grave causada pelo coronavírus2 (SARSCoV2) co meçou no final de 2019 e se espalhou pelo mundo, infectando milhões de pessoas, com mais de 5 milhões de mortes no planeta. Para combater o vírus, é necessário entender como funcionam as suas principais estruturas, principalmente as responsáveis pela patogenicidade da infecção viral. Entender, do ponto de vista físico, a estrutura e as propriedades do SARSCoV2 é extre mamente importante para encontrar pontos vulneráveis que possam elucidar os mecanismos de direcionamento de medicamentos, por exemplo. A determinação da ultraestrutura e das propri edades nanomecânicas do SARSCoV2 pode classificar suas propriedades mecânicas e lançar luz sobre novas rotas de tratamento. Este trabalho tem a Microscopia de Força Atômica como a principal ferramenta para a investigação da ultraestrutura e propriedades nanomecânicas do vírion inativado de SARSCoV2. As partículas virais de SARSCoV2 foram analisadas, com foco especial em sua ultraestrutura, conformação de adsorção e comportamento nanomecânico. Os resultados revelaram aspectos da organização e distribuição espacial das proteínas na super fície das partículas virais. Também foi demonstrado o comportamento complacente e maleável da membrana e sua capacidade de recuperação de lesões mecânicas. Foram medidas pelo menos três camadas que compõem a membrana e sua espessura. Este estudo fornece uma visão singular sobre a ultraestrutura das partículas SARSCoV2 em nanoescala, trazendo novas perspectivas que podem ser empregadas no mapeamento da superfície viral.Submitted by Maria Aparecida (cidazen@gmail.com) on 2023-02-03T13:37:02Z No. of bitstreams: 1 RUANA CARDOSO LIMAvec.pdf: 50676823 bytes, checksum: fe76ef9a92a6f796bbcf348c7e1a883d (MD5)Made available in DSpace on 2023-02-03T13:37:02Z (GMT). No. of bitstreams: 1 RUANA CARDOSO LIMAvec.pdf: 50676823 bytes, checksum: fe76ef9a92a6f796bbcf348c7e1a883d (MD5) Previous issue date: 2022-02-21CAPESapplication/pdfporUniversidade Federal do MaranhãoPROGRAMA DE PÓS-GRADUAÇÃO EM FÍSICA/CCETUFMABrasilDEPARTAMENTO DE FÍSICA/CCETSARSCov2;Biofísica;Nanomecânica;Virologia Física;Microscopia de Força AtômicaSARSCov2;Biophysics;Nanomechanics;Physical Virology;Atomic Force MicroscopyPropriedades de Partículas Específicas e RessonânciasANÁLISE ULTRAESTRUTURAL E NANOMECÂNICA DO VÍRUS SARS-CoV-2ULTRASTRUCTURAL AND NANOMECHANICAL ANALYSIS OF THE SARS-CoV-2 VIRUSinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFMAinstname:Universidade Federal do Maranhão (UFMA)instacron:UFMAORIGINALRUANA CARDOSO LIMAvec.pdfRUANA CARDOSO LIMAvec.pdfapplication/pdf50676823http://tedebc.ufma.br:8080/bitstream/tede/4514/2/RUANA+CARDOSO+LIMAvec.pdffe76ef9a92a6f796bbcf348c7e1a883dMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82255http://tedebc.ufma.br:8080/bitstream/tede/4514/1/license.txt97eeade1fce43278e63fe063657f8083MD51tede/45142023-02-03 10:37:02.617oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttps://tedebc.ufma.br/jspui/PUBhttp://tedebc.ufma.br:8080/oai/requestrepositorio@ufma.br||repositorio@ufma.bropendoar:21312023-02-03T13:37:02Biblioteca Digital de Teses e Dissertações da UFMA - Universidade Federal do Maranhão (UFMA)false |
dc.title.por.fl_str_mv |
ANÁLISE ULTRAESTRUTURAL E NANOMECÂNICA DO VÍRUS SARS-CoV-2 |
dc.title.alternative.eng.fl_str_mv |
ULTRASTRUCTURAL AND NANOMECHANICAL ANALYSIS OF THE SARS-CoV-2 VIRUS |
title |
ANÁLISE ULTRAESTRUTURAL E NANOMECÂNICA DO VÍRUS SARS-CoV-2 |
spellingShingle |
ANÁLISE ULTRAESTRUTURAL E NANOMECÂNICA DO VÍRUS SARS-CoV-2 LIMA, Ruana Cardoso SARSCov2; Biofísica; Nanomecânica; Virologia Física; Microscopia de Força Atômica SARSCov2; Biophysics; Nanomechanics; Physical Virology; Atomic Force Microscopy Propriedades de Partículas Específicas e Ressonâncias |
title_short |
ANÁLISE ULTRAESTRUTURAL E NANOMECÂNICA DO VÍRUS SARS-CoV-2 |
title_full |
ANÁLISE ULTRAESTRUTURAL E NANOMECÂNICA DO VÍRUS SARS-CoV-2 |
title_fullStr |
ANÁLISE ULTRAESTRUTURAL E NANOMECÂNICA DO VÍRUS SARS-CoV-2 |
title_full_unstemmed |
ANÁLISE ULTRAESTRUTURAL E NANOMECÂNICA DO VÍRUS SARS-CoV-2 |
title_sort |
ANÁLISE ULTRAESTRUTURAL E NANOMECÂNICA DO VÍRUS SARS-CoV-2 |
author |
LIMA, Ruana Cardoso |
author_facet |
LIMA, Ruana Cardoso |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
ALENCAR, Luciana Magalhães Rebelo |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1109849519017980 |
dc.contributor.advisor-co1.fl_str_mv |
SANTOS-OLIVEIRA, Ralph |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/2376670019296363 |
dc.contributor.referee1.fl_str_mv |
ALENCAR, Luciana Magalhães Rebelo |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/1109849519017980 |
dc.contributor.referee2.fl_str_mv |
SANTOSOLIVEIRA, Ralph |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/2376670019296363 |
dc.contributor.referee3.fl_str_mv |
MENEZES, Alan Silva de |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/0383834501086115 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9956397727510664 |
dc.contributor.author.fl_str_mv |
LIMA, Ruana Cardoso |
contributor_str_mv |
ALENCAR, Luciana Magalhães Rebelo SANTOS-OLIVEIRA, Ralph ALENCAR, Luciana Magalhães Rebelo SANTOSOLIVEIRA, Ralph MENEZES, Alan Silva de |
dc.subject.por.fl_str_mv |
SARSCov2; Biofísica; Nanomecânica; Virologia Física; Microscopia de Força Atômica |
topic |
SARSCov2; Biofísica; Nanomecânica; Virologia Física; Microscopia de Força Atômica SARSCov2; Biophysics; Nanomechanics; Physical Virology; Atomic Force Microscopy Propriedades de Partículas Específicas e Ressonâncias |
dc.subject.eng.fl_str_mv |
SARSCov2; Biophysics; Nanomechanics; Physical Virology; Atomic Force Microscopy |
dc.subject.cnpq.fl_str_mv |
Propriedades de Partículas Específicas e Ressonâncias |
description |
The ongoing outbreak of the severe acute respiratory syndrome coronavirus2 (SARSCoV2) started in later 2019 and spread across the world, infecting millions of people with over 5 million deaths worldwide. To fight back the virus is necessary to understand how its main structures function, especially those responsible for the virus infectivity pathogenicity. Understanding from the physical point of view the structure and properties of SARSCoV2 is extremely impor tant to find vulnerable points that can elucidate the mechanisms of drug targeting, for example. Determining the ultrastructure and nanomechanical properties of SARSCoV2 can clasifiy its mechanical properties and shed light on new treatment routes. This study has Atomic Force Microscopy as the main tool for the investigation of the ultrastructure and nanomechanical pro perties of the SARSCoV2 inactivated virion. Here, using the most advanced Atomic Force Microscopy techniques, SARSCoV2 viral particles were analyzed, with an especial focus on their ultrastructure, adsorption conformation and nanomechanical behavior. The results uncove red aspects of the organization and the spatial distribution of the proteins on the surface of the viral particles. It was also shown the compliant behavior of the membrane and ability to reco ver from mechanical injuries. At least three layers composing the membrane and their thickness were measured. This study provides new insight into the ultrastructure of SARSCoV2 particles at the nanoscale offering new prospects that could be employed for mapping viral surface. |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022-02-21 |
dc.date.accessioned.fl_str_mv |
2023-02-03T13:37:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
LIMA, Ruana Cardoso. Análise ultraestrutural e nanomecânica do vírus SARS-CoV-2. 2022. 106 f. Dissertação( Programa de Pós-graduação em Física/CCET) - Universidade Federal do Maranhão, São Luís,2022. |
dc.identifier.uri.fl_str_mv |
https://tedebc.ufma.br/jspui/handle/tede/tede/4514 |
identifier_str_mv |
LIMA, Ruana Cardoso. Análise ultraestrutural e nanomecânica do vírus SARS-CoV-2. 2022. 106 f. Dissertação( Programa de Pós-graduação em Física/CCET) - Universidade Federal do Maranhão, São Luís,2022. |
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https://tedebc.ufma.br/jspui/handle/tede/tede/4514 |
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por |
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por |
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openAccess |
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Universidade Federal do Maranhão |
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PROGRAMA DE PÓS-GRADUAÇÃO EM FÍSICA/CCET |
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UFMA |
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Brasil |
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DEPARTAMENTO DE FÍSICA/CCET |
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Universidade Federal do Maranhão |
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