Biologia reprodutiva e expressão gênica gonadal em Genyatremus luteus (BLOCH, 1790) - (Teleostei, Perciformes: Haemulidae)
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFMA |
Texto Completo: | https://tedebc.ufma.br/jspui/handle/tede/tede/4759 |
Resumo: | Genyatremus luteus, popularly known as stonefish, is an estuarine-marine species with a preferentially coastal habitat, living especially in estuaries and lagoons. This species has great potential for extractive fisheries in the state of Maranhão. However, little is known about the reproductive biology of this species. Thus, the objective of this work was to evaluate the reproductive biology and gonadal gene expression in G. luteus on the island of Maranhão. 21 male and 28 female specimens were used. According to the histological characteristics of the testes observed in the histological analysis, males were grouped into three reproductive stages: immature (n=7), maturing (n=7) and mature (n=7) and females, in turn, based on the histological characteristics of the ovarian follicles and the development of the oocyte were also classified: immature (n=7), maturing (n=7), mature (n=7) and spawned (n=7). Plasma levels of hormones E2, 11-KT and 17α-OHP were measured by enzyme immunoassay (ELISA). Estrogen (er) and LH (lhr) receptor gene expression was analyzed by real-time PCR. In males, the plasma concentration of 11-KT showed a peak in the final maturation phase. The highest plasma concentrations of 17α-OHP were found in the immature stage, which decreased in the subsequent stages. On the other hand, 17β-estradiol (E2) showed higher concentration in the maturation stage. The expression of er significantly increased along the maturational development of the testes. The mRNA for the LH receptor decreased from the immature stage to the maturation stage with a consequent peak of expression in the mature stage. There was a high correlation between receptor gene expression and plasma steroid levels, especially with E2. In females, the highest plasma concentration of E2 was found in mature specimens. Plasma concentrations of 17α-OHP increased significantly from the immature stage to the final stage of ovarian maturation. The maturational reproductive stage had no effect on plasma 11-KT concentrations. The expression of er in the ovaries significantly increased throughout ovarian maturation and remained high in the maturation and spawning stages. The expression of lhr showed a similar trend throughout the maturation process, although it reached its peak in the spawning phase. In conclusion, this study characterized for the first time, under the cellular, endocrine and molecular aspects, the different stages of reproductive maturation in males and females of G. luteus, demonstrating that the gene expression profile for er and lhr receptors, as well as plasma concentrations of 11-KT and E2 were directly related to testicular and ovarian maturation, although not necessarily related to the gonadosomatic index. |
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TORRES JUNIOR, José Ribamar de Souzahttp://lattes.cnpq.br/7462697632109147TORRES JUNIOR, José Ribamar de Souzahttp://lattes.cnpq.br/7462697632109147SILVA, Ana Lúcia Abreuhttp://lattes.cnpq.br/8288733951324759CARVALHO NETA, Raimunda Nonata Forteshttp://lattes.cnpq.br/9142178397070079BEZERRA, Nancyleni Pinto Chaveshttp://lattes.cnpq.br/7603276259449956SOUSA, Joicy Cortez de Sáhttp://lattes.cnpq.br/2368453114845145http://lattes.cnpq.br/9697727200577929RIBEIRO, Diego Luiz Dos Santos2023-06-07T13:28:41Z2022-08-25RIBEIRO, Diego Luiz Dos Santos. Biologia reprodutiva e expressão gênica gonadal em Genyatremus luteus (BLOCH, 1790) - (Teleostei, Perciformes: Haemulidae). 2022. 102 f. Tese (Programa de Pós-Graduação em Rede - Rede de Biodiversidade e Biotecnologia da Amazônia Legal/CCBS) - Universidade Federal do Maranhão, São Luís, 2022.https://tedebc.ufma.br/jspui/handle/tede/tede/4759Genyatremus luteus, popularly known as stonefish, is an estuarine-marine species with a preferentially coastal habitat, living especially in estuaries and lagoons. This species has great potential for extractive fisheries in the state of Maranhão. However, little is known about the reproductive biology of this species. Thus, the objective of this work was to evaluate the reproductive biology and gonadal gene expression in G. luteus on the island of Maranhão. 21 male and 28 female specimens were used. According to the histological characteristics of the testes observed in the histological analysis, males were grouped into three reproductive stages: immature (n=7), maturing (n=7) and mature (n=7) and females, in turn, based on the histological characteristics of the ovarian follicles and the development of the oocyte were also classified: immature (n=7), maturing (n=7), mature (n=7) and spawned (n=7). Plasma levels of hormones E2, 11-KT and 17α-OHP were measured by enzyme immunoassay (ELISA). Estrogen (er) and LH (lhr) receptor gene expression was analyzed by real-time PCR. In males, the plasma concentration of 11-KT showed a peak in the final maturation phase. The highest plasma concentrations of 17α-OHP were found in the immature stage, which decreased in the subsequent stages. On the other hand, 17β-estradiol (E2) showed higher concentration in the maturation stage. The expression of er significantly increased along the maturational development of the testes. The mRNA for the LH receptor decreased from the immature stage to the maturation stage with a consequent peak of expression in the mature stage. There was a high correlation between receptor gene expression and plasma steroid levels, especially with E2. In females, the highest plasma concentration of E2 was found in mature specimens. Plasma concentrations of 17α-OHP increased significantly from the immature stage to the final stage of ovarian maturation. The maturational reproductive stage had no effect on plasma 11-KT concentrations. The expression of er in the ovaries significantly increased throughout ovarian maturation and remained high in the maturation and spawning stages. The expression of lhr showed a similar trend throughout the maturation process, although it reached its peak in the spawning phase. In conclusion, this study characterized for the first time, under the cellular, endocrine and molecular aspects, the different stages of reproductive maturation in males and females of G. luteus, demonstrating that the gene expression profile for er and lhr receptors, as well as plasma concentrations of 11-KT and E2 were directly related to testicular and ovarian maturation, although not necessarily related to the gonadosomatic index.Genyatremus luteus, conhecido popularmente como peixe-pedra, é uma espécie estuarino- marinha, de habitat preferencialmente costeiro, vivendo especialmente em estuários e lagunas. Essa espécie tem grande potencial para a exploração pesqueira extrativista no estado do Maranhão. Porém, pouco se sabe sobre a biologia reprodutiva nessa espécie. Desta forma, objetivou-se com o trabalho avaliar a biologia reprodutiva e expressão gênica gonadal em G. luteus na Ilha do Maranhão. Foram utilizados 21 exemplares machos e 28 fêmeas. De acordo com as caraterísticas histológicas dos testículos observada na análise histológica, os machos foram agrupados em três estádios reprodutivos: imaturos (n=7), em maturação (n=7) e maduros (n=7) e asfêmeas, por sua vez, com base nas características histológicas dos folículos ovarianos e no desenvolvimento do oócito foram também classificadas: imaturas (n=7), em maturação (n=7), maduro (n=7) e desovado (n=7). Os níveis plasmáticos dos hormônios E2, 11-KT e 17α- OHP foram medidos por meio de ensaio imunoenzimático (ELISA). A expressão gênica dos receptores de estrogênio (er) e LH (lhr) foi analisada por PCR em tempo real. Nos machos, a concentração plasmática de 11-KT apresentou um pico na fase de maturação final. As maiores concentrações plasmáticas de 17α-OHP foram encontradas no estádio imaturo, as quais diminuiram nos estágios subsequentes. Por outro lado, o 17β-estradiol (E2) apresentou maior concentração no estádio em maturação. A expressão de er aumentou significativamente ao longo do desenvolvimento maturacional dos testículos. O mRNA para o receptor de LH diminuiu do estádio imaturo para o estádio em maturação com consequente pico de expressão no estádio maduro. Houve alta correlação entre a expressão gênica dos receptores e os níveis de esteróides plasmáticos, especialmente com E2. Nas fêmeas, a maior concentração plasmática de E2 foi encontrada em espécimes maduros. As concentrações plasmáticas de 17α-OHP aumentaram significativamente desde o estágio imaturo até o estágio final de maturação ovariano. O estágio reprodutivo maturacional não teve nenhum efeito nas concentrações plasmáticas de 11-KT. A expressão de er nos ovários aumentou significativamente ao longo da maturação ovariana e permaneceu alta nos estágios de maturação e desova. A expressão de lhr apresentou tendência semelhante ao longo do processo de maturação, embora tenha atingido seu pico na fase de desova. Em conclusão, este estudo caracterizou pela primeira vez, sob os aspectos celular, endócrino e molecular, os diferentes estádios de maturação reprodutiva em machos e fêmeas de G. luteus, demonstrando que o perfil da expressão gênica para osreceptores er e lhr, bem como as concentrações plasmáticas de 11-KT e E2 foram diretamente relacionados à maturação testicular e ovariana, apesar de não se relacionarem necessariamente com o índice gonadossomático.Submitted by Jonathan Sousa de Almeida (jonathan.sousa@ufma.br) on 2023-06-07T13:28:41Z No. of bitstreams: 1 DIEGOLUIZDOSSANTOSRIBEIRO.pdf: 2101961 bytes, checksum: dafce2a658a4b64f1c04fe0d090fa331 (MD5)Made available in DSpace on 2023-06-07T13:28:41Z (GMT). No. of bitstreams: 1 DIEGOLUIZDOSSANTOSRIBEIRO.pdf: 2101961 bytes, checksum: dafce2a658a4b64f1c04fe0d090fa331 (MD5) Previous issue date: 2022-08-25FAPEMAapplication/pdfporUniversidade Federal do MaranhãoPROGRAMA DE PÓS-GRADUAÇÃO EM REDE - REDE DE BIODIVERSIDADE E BIOTECNOLOGIA DA AMAZÔNIA LEGAL/CCBSUFMABrasilDEPARTAMENTO DE OCEANOGRAFIA E LIMNOLOGIA/CCBSroncador;receptor de gonadotrofinasestradiol;gônadas;reprodução.grunts;gonadotropin receptor;estradiol;gonads;reproduction.Reprodução AnimalEngenharia de PescaBiologia reprodutiva e expressão gênica gonadal em Genyatremus luteus (BLOCH, 1790) - (Teleostei, Perciformes: Haemulidae)Reproductive biology and gonadal gene expression in Genyatremus luteus (BLOCH, 1790) - (Teleostei, Perciformes: Haemulidae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFMAinstname:Universidade Federal do Maranhão (UFMA)instacron:UFMAORIGINALDIEGOLUIZDOSSANTOSRIBEIRO.pdfDIEGOLUIZDOSSANTOSRIBEIRO.pdfapplication/pdf2101961http://tedebc.ufma.br:8080/bitstream/tede/4759/2/DIEGOLUIZDOSSANTOSRIBEIRO.pdfdafce2a658a4b64f1c04fe0d090fa331MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82255http://tedebc.ufma.br:8080/bitstream/tede/4759/1/license.txt97eeade1fce43278e63fe063657f8083MD51tede/47592023-06-07 10:54:09.763oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttps://tedebc.ufma.br/jspui/PUBhttp://tedebc.ufma.br:8080/oai/requestrepositorio@ufma.br||repositorio@ufma.bropendoar:21312023-06-07T13:54:09Biblioteca Digital de Teses e Dissertações da UFMA - Universidade Federal do Maranhão (UFMA)false |
dc.title.por.fl_str_mv |
Biologia reprodutiva e expressão gênica gonadal em Genyatremus luteus (BLOCH, 1790) - (Teleostei, Perciformes: Haemulidae) |
dc.title.alternative.eng.fl_str_mv |
Reproductive biology and gonadal gene expression in Genyatremus luteus (BLOCH, 1790) - (Teleostei, Perciformes: Haemulidae) |
title |
Biologia reprodutiva e expressão gênica gonadal em Genyatremus luteus (BLOCH, 1790) - (Teleostei, Perciformes: Haemulidae) |
spellingShingle |
Biologia reprodutiva e expressão gênica gonadal em Genyatremus luteus (BLOCH, 1790) - (Teleostei, Perciformes: Haemulidae) RIBEIRO, Diego Luiz Dos Santos roncador; receptor de gonadotrofinas estradiol; gônadas; reprodução. grunts; gonadotropin receptor; estradiol; gonads; reproduction. Reprodução Animal Engenharia de Pesca |
title_short |
Biologia reprodutiva e expressão gênica gonadal em Genyatremus luteus (BLOCH, 1790) - (Teleostei, Perciformes: Haemulidae) |
title_full |
Biologia reprodutiva e expressão gênica gonadal em Genyatremus luteus (BLOCH, 1790) - (Teleostei, Perciformes: Haemulidae) |
title_fullStr |
Biologia reprodutiva e expressão gênica gonadal em Genyatremus luteus (BLOCH, 1790) - (Teleostei, Perciformes: Haemulidae) |
title_full_unstemmed |
Biologia reprodutiva e expressão gênica gonadal em Genyatremus luteus (BLOCH, 1790) - (Teleostei, Perciformes: Haemulidae) |
title_sort |
Biologia reprodutiva e expressão gênica gonadal em Genyatremus luteus (BLOCH, 1790) - (Teleostei, Perciformes: Haemulidae) |
author |
RIBEIRO, Diego Luiz Dos Santos |
author_facet |
RIBEIRO, Diego Luiz Dos Santos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
TORRES JUNIOR, José Ribamar de Souza |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7462697632109147 |
dc.contributor.referee1.fl_str_mv |
TORRES JUNIOR, José Ribamar de Souza |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/7462697632109147 |
dc.contributor.referee2.fl_str_mv |
SILVA, Ana Lúcia Abreu |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/8288733951324759 |
dc.contributor.referee3.fl_str_mv |
CARVALHO NETA, Raimunda Nonata Fortes |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/9142178397070079 |
dc.contributor.referee4.fl_str_mv |
BEZERRA, Nancyleni Pinto Chaves |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/7603276259449956 |
dc.contributor.referee5.fl_str_mv |
SOUSA, Joicy Cortez de Sá |
dc.contributor.referee5Lattes.fl_str_mv |
http://lattes.cnpq.br/2368453114845145 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9697727200577929 |
dc.contributor.author.fl_str_mv |
RIBEIRO, Diego Luiz Dos Santos |
contributor_str_mv |
TORRES JUNIOR, José Ribamar de Souza TORRES JUNIOR, José Ribamar de Souza SILVA, Ana Lúcia Abreu CARVALHO NETA, Raimunda Nonata Fortes BEZERRA, Nancyleni Pinto Chaves SOUSA, Joicy Cortez de Sá |
dc.subject.por.fl_str_mv |
roncador; receptor de gonadotrofinas estradiol; gônadas; reprodução. |
topic |
roncador; receptor de gonadotrofinas estradiol; gônadas; reprodução. grunts; gonadotropin receptor; estradiol; gonads; reproduction. Reprodução Animal Engenharia de Pesca |
dc.subject.eng.fl_str_mv |
grunts; gonadotropin receptor; estradiol; gonads; reproduction. |
dc.subject.cnpq.fl_str_mv |
Reprodução Animal Engenharia de Pesca |
description |
Genyatremus luteus, popularly known as stonefish, is an estuarine-marine species with a preferentially coastal habitat, living especially in estuaries and lagoons. This species has great potential for extractive fisheries in the state of Maranhão. However, little is known about the reproductive biology of this species. Thus, the objective of this work was to evaluate the reproductive biology and gonadal gene expression in G. luteus on the island of Maranhão. 21 male and 28 female specimens were used. According to the histological characteristics of the testes observed in the histological analysis, males were grouped into three reproductive stages: immature (n=7), maturing (n=7) and mature (n=7) and females, in turn, based on the histological characteristics of the ovarian follicles and the development of the oocyte were also classified: immature (n=7), maturing (n=7), mature (n=7) and spawned (n=7). Plasma levels of hormones E2, 11-KT and 17α-OHP were measured by enzyme immunoassay (ELISA). Estrogen (er) and LH (lhr) receptor gene expression was analyzed by real-time PCR. In males, the plasma concentration of 11-KT showed a peak in the final maturation phase. The highest plasma concentrations of 17α-OHP were found in the immature stage, which decreased in the subsequent stages. On the other hand, 17β-estradiol (E2) showed higher concentration in the maturation stage. The expression of er significantly increased along the maturational development of the testes. The mRNA for the LH receptor decreased from the immature stage to the maturation stage with a consequent peak of expression in the mature stage. There was a high correlation between receptor gene expression and plasma steroid levels, especially with E2. In females, the highest plasma concentration of E2 was found in mature specimens. Plasma concentrations of 17α-OHP increased significantly from the immature stage to the final stage of ovarian maturation. The maturational reproductive stage had no effect on plasma 11-KT concentrations. The expression of er in the ovaries significantly increased throughout ovarian maturation and remained high in the maturation and spawning stages. The expression of lhr showed a similar trend throughout the maturation process, although it reached its peak in the spawning phase. In conclusion, this study characterized for the first time, under the cellular, endocrine and molecular aspects, the different stages of reproductive maturation in males and females of G. luteus, demonstrating that the gene expression profile for er and lhr receptors, as well as plasma concentrations of 11-KT and E2 were directly related to testicular and ovarian maturation, although not necessarily related to the gonadosomatic index. |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022-08-25 |
dc.date.accessioned.fl_str_mv |
2023-06-07T13:28:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RIBEIRO, Diego Luiz Dos Santos. Biologia reprodutiva e expressão gênica gonadal em Genyatremus luteus (BLOCH, 1790) - (Teleostei, Perciformes: Haemulidae). 2022. 102 f. Tese (Programa de Pós-Graduação em Rede - Rede de Biodiversidade e Biotecnologia da Amazônia Legal/CCBS) - Universidade Federal do Maranhão, São Luís, 2022. |
dc.identifier.uri.fl_str_mv |
https://tedebc.ufma.br/jspui/handle/tede/tede/4759 |
identifier_str_mv |
RIBEIRO, Diego Luiz Dos Santos. Biologia reprodutiva e expressão gênica gonadal em Genyatremus luteus (BLOCH, 1790) - (Teleostei, Perciformes: Haemulidae). 2022. 102 f. Tese (Programa de Pós-Graduação em Rede - Rede de Biodiversidade e Biotecnologia da Amazônia Legal/CCBS) - Universidade Federal do Maranhão, São Luís, 2022. |
url |
https://tedebc.ufma.br/jspui/handle/tede/tede/4759 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal do Maranhão |
dc.publisher.program.fl_str_mv |
PROGRAMA DE PÓS-GRADUAÇÃO EM REDE - REDE DE BIODIVERSIDADE E BIOTECNOLOGIA DA AMAZÔNIA LEGAL/CCBS |
dc.publisher.initials.fl_str_mv |
UFMA |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
DEPARTAMENTO DE OCEANOGRAFIA E LIMNOLOGIA/CCBS |
publisher.none.fl_str_mv |
Universidade Federal do Maranhão |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UFMA instname:Universidade Federal do Maranhão (UFMA) instacron:UFMA |
instname_str |
Universidade Federal do Maranhão (UFMA) |
instacron_str |
UFMA |
institution |
UFMA |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UFMA |
collection |
Biblioteca Digital de Teses e Dissertações da UFMA |
bitstream.url.fl_str_mv |
http://tedebc.ufma.br:8080/bitstream/tede/4759/2/DIEGOLUIZDOSSANTOSRIBEIRO.pdf http://tedebc.ufma.br:8080/bitstream/tede/4759/1/license.txt |
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dafce2a658a4b64f1c04fe0d090fa331 97eeade1fce43278e63fe063657f8083 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UFMA - Universidade Federal do Maranhão (UFMA) |
repository.mail.fl_str_mv |
repositorio@ufma.br||repositorio@ufma.br |
_version_ |
1809926214547144704 |