Atividade osteoindutora da fotobiomodulação nos comprimentos de onda vermelho e infravermelho em modelo ex vivo de remodelação óssea

Detalhes bibliográficos
Autor(a) principal: FURTADO, Guilherme Silva
Data de Publicação: 2023
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFMA
Texto Completo: https://tedebc.ufma.br/jspui/handle/tede/tede/5137
Resumo: Introduction: Photobiomodulation therapy (PBM) utilizes non-ionizing light at specific wavelengths to activate endogenous chromophores, modulating metabolic pathways, and inducing beneficial biological outcomes. Focused on wavelengths such as blue violet, green, red, and near-infrared, PBM has been applied in various therapeutic conditions, including brain injuries, wound healing, and bone regeneration. Objective: The study assessed the effects of PBM on bone remodeling in a physiological setting, using an ex vivo model of chicken embryonic femur. The research aimed to comprehend the molecular and cellular impacts of PBM on bone tissue and fill gaps in its efficacy and application protocols. Methodology: 5 fertilized chicken eggs (Gallus domesticus) were incubated in the Octagon 40 ECO egg incubator (Brinsea, UK) at a temperature of 37°C and 50% humidity. On the 11th day, the embryos were sacrificed, and the whole femurs were carefully dissected, removing soft tissues such as ligaments and adherent muscles. The femurs were then placed in Netwell culture inserts, which have a polyester membrane with pores of 440μm and 30mm in diameter (Corning, Arizona, USA). The femurs were cultured in 6-well tissue culture plates (Corning, Arizona, USA) containing 1mL of basal culture medium, composed of 1mL of αMEM, 100U/mL penicillin, 100μg/mL streptomycin, 2.5μg/mL amphotericin B, 50 μg/mL ascorbic acid 2-phosphate (all from Sigma). The femurs were divided into control and treatment groups (n=5). In the treatment groups, low-power diode laser irradiation was performed using red (660nm) and infrared (808nm) wavelengths. Continuous irradiation mode and a dose of 33.33J/cm2 were applied, corresponding to 10s, 100mW, so that each specimen received 1.0J of energy dose per irradiation. The femurs were irradiated four times during the 11 days of culture - immediately after dissection and 24 hours, 48 hours, and 7 days after the first irradiation. As a standardization measure, a distance of 1mm was defined between the laser source and the samples to avoid light dissipation and maximize energy absorption. The analysis was conducted on day 11, involving histochemical staining, histological analysis, and gene expression. Results: Irradiated femurs showed an increase in the mineralized area, suggesting higher osteoblastic activity at both wavelengths. Histological analysis indicated increased collagen deposition and maturation in the irradiated groups, with more pronounced effects in the infrared group. Gene expression revealed a significant increase in RUNX2 (Runt-related transcription factor 2) and SOX9 (SRY-like HMG [High-Mobility Group] Box 9), indicating enhanced osteochondrogenic commitment, along with a trend of reduction in SOST (Sclerostin). Conclusion: The results suggest that PBM, especially in red and infrared wavelengths, not only preserves bone structure but actively promotes bone formation under physiological conditions. This study provides a solid foundation for future investigations into the clinical application of PBM in bone regeneration.
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spelling LAGO, Andréa Dias Neveshttp://lattes.cnpq.br/4289519113988271LAGO, Andréa Dias Neveshttp://lattes.cnpq.br/4289519113988271RODRIGUES, Fernanda Cristina Nogueirahttp://lattes.cnpq.br/8627330604562091MARQUES, Daniele Meira Condehttp://lattes.cnpq.br/6988763738175457MORAES, Marcia Cristina Dias dehttp://lattes.cnpq.br/6125991234101338CASANOVAS, Rosana Costahttp://lattes.cnpq.br/5037087509686293http://lattes.cnpq.br/9001568191415918FURTADO, Guilherme Silva2024-02-15T14:45:25Z2023-12-27FURTADO, Guilherme Silva. Atividade osteoindutora da fotobiomodulação nos comprimentos de onda vermelho e infravermelho em modelo ex vivo de remodelação óssea. 2023. 75 f. Dissertação( Programa de Pós-Graduação em Odontologia/CCBS) - Universidade Federal do Maranhão, São Luís, 2023.https://tedebc.ufma.br/jspui/handle/tede/tede/5137Introduction: Photobiomodulation therapy (PBM) utilizes non-ionizing light at specific wavelengths to activate endogenous chromophores, modulating metabolic pathways, and inducing beneficial biological outcomes. Focused on wavelengths such as blue violet, green, red, and near-infrared, PBM has been applied in various therapeutic conditions, including brain injuries, wound healing, and bone regeneration. Objective: The study assessed the effects of PBM on bone remodeling in a physiological setting, using an ex vivo model of chicken embryonic femur. The research aimed to comprehend the molecular and cellular impacts of PBM on bone tissue and fill gaps in its efficacy and application protocols. Methodology: 5 fertilized chicken eggs (Gallus domesticus) were incubated in the Octagon 40 ECO egg incubator (Brinsea, UK) at a temperature of 37°C and 50% humidity. On the 11th day, the embryos were sacrificed, and the whole femurs were carefully dissected, removing soft tissues such as ligaments and adherent muscles. The femurs were then placed in Netwell culture inserts, which have a polyester membrane with pores of 440μm and 30mm in diameter (Corning, Arizona, USA). The femurs were cultured in 6-well tissue culture plates (Corning, Arizona, USA) containing 1mL of basal culture medium, composed of 1mL of αMEM, 100U/mL penicillin, 100μg/mL streptomycin, 2.5μg/mL amphotericin B, 50 μg/mL ascorbic acid 2-phosphate (all from Sigma). The femurs were divided into control and treatment groups (n=5). In the treatment groups, low-power diode laser irradiation was performed using red (660nm) and infrared (808nm) wavelengths. Continuous irradiation mode and a dose of 33.33J/cm2 were applied, corresponding to 10s, 100mW, so that each specimen received 1.0J of energy dose per irradiation. The femurs were irradiated four times during the 11 days of culture - immediately after dissection and 24 hours, 48 hours, and 7 days after the first irradiation. As a standardization measure, a distance of 1mm was defined between the laser source and the samples to avoid light dissipation and maximize energy absorption. The analysis was conducted on day 11, involving histochemical staining, histological analysis, and gene expression. Results: Irradiated femurs showed an increase in the mineralized area, suggesting higher osteoblastic activity at both wavelengths. Histological analysis indicated increased collagen deposition and maturation in the irradiated groups, with more pronounced effects in the infrared group. Gene expression revealed a significant increase in RUNX2 (Runt-related transcription factor 2) and SOX9 (SRY-like HMG [High-Mobility Group] Box 9), indicating enhanced osteochondrogenic commitment, along with a trend of reduction in SOST (Sclerostin). Conclusion: The results suggest that PBM, especially in red and infrared wavelengths, not only preserves bone structure but actively promotes bone formation under physiological conditions. This study provides a solid foundation for future investigations into the clinical application of PBM in bone regeneration.Introdução: A terapia de fotobiomodulação (TFBM) utiliza luz não ionizante em comprimentos de onda específicos para acionar cromóforos endógenos, modulando vias metabólicas e induzindo resultados biológicos benéficos. Com foco em comprimentos de onda como azul-violeta, verde, vermelho e infravermelho próximo, a TFBM tem sido aplicada em diversas condições terapêuticas, incluindo lesões cerebrais, cicatrização de feridas e regeneração óssea. Objetivo: O estudo avaliou os efeitos da TFBM na remodelação óssea em um cenário fisiológico, utilizando um modelo ex vivo de fêmur embrionário de galinha. A pesquisa buscou compreender os impactos moleculares e celulares da TFBM no tecido ósseo e preencher lacunas sobre sua eficácia e protocolos de aplicação. Metodologia: 5 Ovos fertilizados de galinha (Gallus domesticus) foram cultivados na incubadora rotativa de ovos Octagon 40 ECO (Brinsea, Reino Unido) a uma temperatura de 37°C e 50% de umidade. No 11º dia, os embriões foram sacrificados e os fêmures inteiros foram cuidadosamente dissecados, removendo tecidos moles, como ligamentos e músculos aderentes. Os fêmures foram então colocados em inserções de cultura Netwell, que possuem uma membrana de poliéster com poros de 440μm e 30mm de diâmetro (Corning, Arizona, EUA). Os fêmures foram cultivados em placas de cultura de tecidos de 6 poços (Corning, Arizona, EUA) contendo 1mL de meio de cultura basal, composto por 1mL de αMEM, 100U/mL de penicilina, 100μg/mL de estreptomicina, 2,5μg/mL de anfotericina B, 50 μg/mL de ácido ascórbico 2-fosfato (todos da Sigma). Os fêmures foram divididos em grupos de controle e tratamento (n=5). Nos grupos de tratamento, irradiou-se com um laser de diodo de baixa potência, utilizando luz nos comprimentos de onda vermelho (660nm) e infravermelho (808nm). Foi aplicado um modo de irradiação contínua e uma dose de 33,33J/cm2, o que corresponde a 10s, 100mW, de modo que cada espécime recebeu 1,0J de dose de energia por irradiação. Os fêmures foram irradiados quatro vezes durante os 11 dias de cultura - imediatamente após a dissecção e 24 horas, 48 horas e 7 dias após a primeira irradiação. Como medida de padronização, foi definida uma distância de 1mm entre a fonte de laser e as amostras para evitar a dissipação de luz e a absorção máxima de energia A análise foi realizada em 11 dias, com coloração histoquímica, análise histológica e expressão gênica. Resultados: Os fêmures irradiados mostraram aumento na área mineralizada, sugerindo maior atividade osteoblástica nos dois comprimentos de onda. A análise histológica indicou maior deposição e maturação do colágeno nos grupos irradiados, com efeitos mais pronunciados no infravermelho. A expressão gênica revelou aumento significativo de RUNX2 (Runt-related transcription factor 2) e SOX9 (SRY-like HMG [High-Mobility Group] Box 9), indicando maior compromisso osteocondrogênico, além de uma tendência de redução em SOST (Esclerostina). Conclusão: Os resultados sugerem que a TFBM, especialmente nos comprimentos de onda vermelho e infravermelho, não apenas preserva a estrutura óssea, mas também promove ativamente a formação óssea em condições fisiológicas. Este estudo fornece uma base sólida para futuras investigações sobre a aplicação clínica da TFBM na regeneração óssea.Submitted by Maria Aparecida (cidazen@gmail.com) on 2024-02-15T14:45:25Z No. of bitstreams: 1 Guilherme Furtado.pdf: 22325534 bytes, checksum: 4eca1586e36cb83b4d066d8591d9b2ad (MD5)Made available in DSpace on 2024-02-15T14:45:25Z (GMT). No. of bitstreams: 1 Guilherme Furtado.pdf: 22325534 bytes, checksum: 4eca1586e36cb83b4d066d8591d9b2ad (MD5) Previous issue date: 2023-12-27CAPESapplication/pdfporUniversidade Federal do MaranhãoPROGRAMA DE PÓS-GRADUAÇÃO EM ODONTOLOGIA/CCBSUFMABrasilDEPARTAMENTO DE ODONTOLOGIA I/CCBSFotobiomodulação;remodelação óssea;modelo ex vivo;laser vermelho;laser infravermelhoPhotobiomodulation;bone remodelling;ex vivo model;red laser;infrared laserClínica OdontológicaAtividade osteoindutora da fotobiomodulação nos comprimentos de onda vermelho e infravermelho em modelo ex vivo de remodelação ósseaOsteoinductive activity of photobiomodulation at wavelengths of red and infrared wavelengths in an ex vivo model of bone remodelinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFMAinstname:Universidade Federal do Maranhão (UFMA)instacron:UFMAORIGINALGuilherme Furtado.pdfGuilherme Furtado.pdfapplication/pdf22325534http://tedebc.ufma.br:8080/bitstream/tede/5137/2/Guilherme+Furtado.pdf4eca1586e36cb83b4d066d8591d9b2adMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82255http://tedebc.ufma.br:8080/bitstream/tede/5137/1/license.txt97eeade1fce43278e63fe063657f8083MD51tede/51372024-02-15 11:45:25.465oai:tede2: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Biblioteca Digital de Teses e Dissertaçõeshttps://tedebc.ufma.br/jspui/PUBhttp://tedebc.ufma.br:8080/oai/requestrepositorio@ufma.br||repositorio@ufma.bropendoar:21312024-02-15T14:45:25Biblioteca Digital de Teses e Dissertações da UFMA - Universidade Federal do Maranhão (UFMA)false
dc.title.por.fl_str_mv Atividade osteoindutora da fotobiomodulação nos comprimentos de onda vermelho e infravermelho em modelo ex vivo de remodelação óssea
dc.title.alternative.eng.fl_str_mv Osteoinductive activity of photobiomodulation at wavelengths of red and infrared wavelengths in an ex vivo model of bone remodeling
title Atividade osteoindutora da fotobiomodulação nos comprimentos de onda vermelho e infravermelho em modelo ex vivo de remodelação óssea
spellingShingle Atividade osteoindutora da fotobiomodulação nos comprimentos de onda vermelho e infravermelho em modelo ex vivo de remodelação óssea
FURTADO, Guilherme Silva
Fotobiomodulação;
remodelação óssea;
modelo ex vivo;
laser vermelho;
laser infravermelho
Photobiomodulation;
bone remodelling;
ex vivo model;
red laser;
infrared laser
Clínica Odontológica
title_short Atividade osteoindutora da fotobiomodulação nos comprimentos de onda vermelho e infravermelho em modelo ex vivo de remodelação óssea
title_full Atividade osteoindutora da fotobiomodulação nos comprimentos de onda vermelho e infravermelho em modelo ex vivo de remodelação óssea
title_fullStr Atividade osteoindutora da fotobiomodulação nos comprimentos de onda vermelho e infravermelho em modelo ex vivo de remodelação óssea
title_full_unstemmed Atividade osteoindutora da fotobiomodulação nos comprimentos de onda vermelho e infravermelho em modelo ex vivo de remodelação óssea
title_sort Atividade osteoindutora da fotobiomodulação nos comprimentos de onda vermelho e infravermelho em modelo ex vivo de remodelação óssea
author FURTADO, Guilherme Silva
author_facet FURTADO, Guilherme Silva
author_role author
dc.contributor.advisor1.fl_str_mv LAGO, Andréa Dias Neves
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/4289519113988271
dc.contributor.referee1.fl_str_mv LAGO, Andréa Dias Neves
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/4289519113988271
dc.contributor.referee2.fl_str_mv RODRIGUES, Fernanda Cristina Nogueira
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/8627330604562091
dc.contributor.referee3.fl_str_mv MARQUES, Daniele Meira Conde
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/6988763738175457
dc.contributor.referee4.fl_str_mv MORAES, Marcia Cristina Dias de
dc.contributor.referee4Lattes.fl_str_mv http://lattes.cnpq.br/6125991234101338
dc.contributor.referee5.fl_str_mv CASANOVAS, Rosana Costa
dc.contributor.referee5Lattes.fl_str_mv http://lattes.cnpq.br/5037087509686293
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/9001568191415918
dc.contributor.author.fl_str_mv FURTADO, Guilherme Silva
contributor_str_mv LAGO, Andréa Dias Neves
LAGO, Andréa Dias Neves
RODRIGUES, Fernanda Cristina Nogueira
MARQUES, Daniele Meira Conde
MORAES, Marcia Cristina Dias de
CASANOVAS, Rosana Costa
dc.subject.por.fl_str_mv Fotobiomodulação;
remodelação óssea;
modelo ex vivo;
laser vermelho;
laser infravermelho
topic Fotobiomodulação;
remodelação óssea;
modelo ex vivo;
laser vermelho;
laser infravermelho
Photobiomodulation;
bone remodelling;
ex vivo model;
red laser;
infrared laser
Clínica Odontológica
dc.subject.eng.fl_str_mv Photobiomodulation;
bone remodelling;
ex vivo model;
red laser;
infrared laser
dc.subject.cnpq.fl_str_mv Clínica Odontológica
description Introduction: Photobiomodulation therapy (PBM) utilizes non-ionizing light at specific wavelengths to activate endogenous chromophores, modulating metabolic pathways, and inducing beneficial biological outcomes. Focused on wavelengths such as blue violet, green, red, and near-infrared, PBM has been applied in various therapeutic conditions, including brain injuries, wound healing, and bone regeneration. Objective: The study assessed the effects of PBM on bone remodeling in a physiological setting, using an ex vivo model of chicken embryonic femur. The research aimed to comprehend the molecular and cellular impacts of PBM on bone tissue and fill gaps in its efficacy and application protocols. Methodology: 5 fertilized chicken eggs (Gallus domesticus) were incubated in the Octagon 40 ECO egg incubator (Brinsea, UK) at a temperature of 37°C and 50% humidity. On the 11th day, the embryos were sacrificed, and the whole femurs were carefully dissected, removing soft tissues such as ligaments and adherent muscles. The femurs were then placed in Netwell culture inserts, which have a polyester membrane with pores of 440μm and 30mm in diameter (Corning, Arizona, USA). The femurs were cultured in 6-well tissue culture plates (Corning, Arizona, USA) containing 1mL of basal culture medium, composed of 1mL of αMEM, 100U/mL penicillin, 100μg/mL streptomycin, 2.5μg/mL amphotericin B, 50 μg/mL ascorbic acid 2-phosphate (all from Sigma). The femurs were divided into control and treatment groups (n=5). In the treatment groups, low-power diode laser irradiation was performed using red (660nm) and infrared (808nm) wavelengths. Continuous irradiation mode and a dose of 33.33J/cm2 were applied, corresponding to 10s, 100mW, so that each specimen received 1.0J of energy dose per irradiation. The femurs were irradiated four times during the 11 days of culture - immediately after dissection and 24 hours, 48 hours, and 7 days after the first irradiation. As a standardization measure, a distance of 1mm was defined between the laser source and the samples to avoid light dissipation and maximize energy absorption. The analysis was conducted on day 11, involving histochemical staining, histological analysis, and gene expression. Results: Irradiated femurs showed an increase in the mineralized area, suggesting higher osteoblastic activity at both wavelengths. Histological analysis indicated increased collagen deposition and maturation in the irradiated groups, with more pronounced effects in the infrared group. Gene expression revealed a significant increase in RUNX2 (Runt-related transcription factor 2) and SOX9 (SRY-like HMG [High-Mobility Group] Box 9), indicating enhanced osteochondrogenic commitment, along with a trend of reduction in SOST (Sclerostin). Conclusion: The results suggest that PBM, especially in red and infrared wavelengths, not only preserves bone structure but actively promotes bone formation under physiological conditions. This study provides a solid foundation for future investigations into the clinical application of PBM in bone regeneration.
publishDate 2023
dc.date.issued.fl_str_mv 2023-12-27
dc.date.accessioned.fl_str_mv 2024-02-15T14:45:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv FURTADO, Guilherme Silva. Atividade osteoindutora da fotobiomodulação nos comprimentos de onda vermelho e infravermelho em modelo ex vivo de remodelação óssea. 2023. 75 f. Dissertação( Programa de Pós-Graduação em Odontologia/CCBS) - Universidade Federal do Maranhão, São Luís, 2023.
dc.identifier.uri.fl_str_mv https://tedebc.ufma.br/jspui/handle/tede/tede/5137
identifier_str_mv FURTADO, Guilherme Silva. Atividade osteoindutora da fotobiomodulação nos comprimentos de onda vermelho e infravermelho em modelo ex vivo de remodelação óssea. 2023. 75 f. Dissertação( Programa de Pós-Graduação em Odontologia/CCBS) - Universidade Federal do Maranhão, São Luís, 2023.
url https://tedebc.ufma.br/jspui/handle/tede/tede/5137
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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