High positivity values for bovine leukemia virus in human breast cancer cases from Minas Gerais, Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | http://hdl.handle.net/1843/41159 |
Resumo: | Bovine leukemia virus (BLV) is a retrovirus that causes lymphoma in cattle worldwide and has also been associated with breast cancer in humans. The mechanism of BLV infection in humans and its implication as a primary cause of cancer in women are not known yet. BLV infection in humans may be caused by the consumption of milk and milk-products or meat from infected animals. Breast cancer incidence rates in Brazil are high, corresponding to 29.5% a year of cancer cases among women. In 2020, an estimated 66,280 new cases of breast cancer are expected, whereas in 2018 breast cancer has led to 17,572 deaths, the highest incidence and lethality among cancers in women in this country that year. BLV infection occurrence ranges from 60 to 95% in dairy herds. In addition, there are some regions, such as the Minas Gerais State, southeastern Brazil, where the population traditionally consume unpasteurized dairy products. Taken together, this study aimed to verify if there is a higher association between breast cancer and the presence of BLV genome in breast tissue samples within this population that consumes raw milk from animals with high rates of BLV infection. A molecular study of two BLV genes was carried out in 88 breast parenchyma samples, between tumors and controls. The amplified fragment was subjected to BLV proviral sequencing and its identity was confirmed using GenBank. BLV proviral genes were amplified from tumor breast parenchyma samples and healthy tissue control samples from women, revealing a 95.9% (47/49) and 59% (23/39) positivity, respectively. Our results show the highest correlation of BLV and human breast cancer found in the world to date within the population of Minas Gerais, Brazil. |
id |
UFMG_0d91b16ceb3e8401a2864b3c706a8ba1 |
---|---|
oai_identifier_str |
oai:repositorio.ufmg.br:1843/41159 |
network_acronym_str |
UFMG |
network_name_str |
Repositório Institucional da UFMG |
repository_id_str |
|
spelling |
2022-04-26T19:52:50Z2022-04-26T19:52:50Z2020-10-05151011210.1371/journal.pone.023974519326203http://hdl.handle.net/1843/411590000-0002-5266-6870Bovine leukemia virus (BLV) is a retrovirus that causes lymphoma in cattle worldwide and has also been associated with breast cancer in humans. The mechanism of BLV infection in humans and its implication as a primary cause of cancer in women are not known yet. BLV infection in humans may be caused by the consumption of milk and milk-products or meat from infected animals. Breast cancer incidence rates in Brazil are high, corresponding to 29.5% a year of cancer cases among women. In 2020, an estimated 66,280 new cases of breast cancer are expected, whereas in 2018 breast cancer has led to 17,572 deaths, the highest incidence and lethality among cancers in women in this country that year. BLV infection occurrence ranges from 60 to 95% in dairy herds. In addition, there are some regions, such as the Minas Gerais State, southeastern Brazil, where the population traditionally consume unpasteurized dairy products. Taken together, this study aimed to verify if there is a higher association between breast cancer and the presence of BLV genome in breast tissue samples within this population that consumes raw milk from animals with high rates of BLV infection. A molecular study of two BLV genes was carried out in 88 breast parenchyma samples, between tumors and controls. The amplified fragment was subjected to BLV proviral sequencing and its identity was confirmed using GenBank. BLV proviral genes were amplified from tumor breast parenchyma samples and healthy tissue control samples from women, revealing a 95.9% (47/49) and 59% (23/39) positivity, respectively. Our results show the highest correlation of BLV and human breast cancer found in the world to date within the population of Minas Gerais, Brazil.O vírus da leucemia bovina (BLV) é um retrovírus que causa linfoma em bovinos em todo o mundo e também tem sido associada ao câncer de mama em humanos. O mecanismo de infecção pelo BLV em humanos e sua implicação como causa primária de câncer em mulheres ainda não são conhecidos. BLV infecção em humanos pode ser causada pelo consumo de leite e produtos lácteos ou carne de animais infectados. As taxas de incidência de câncer de mama no Brasil são altas, correspondendo a 29,5% ao ano de casos de câncer entre as mulheres. Em 2020, estima-se que 66.280 novos casos de câncer de mama são esperados, enquanto em 2018 o câncer de mama levou a 17.572 mortes, o maior incidência e letalidade entre os cânceres em mulheres neste país naquele ano. A o ocorrência de infecção por BLV varia de 60 a 95% em rebanhos leiteiros. Além disso, existem algumas regiões, como o Estado de Minas Gerais, sudeste do Brasil, onde a população tradicionalmente consome produtos lácteos não pasteurizados. Em conjunto, este estudo teve como objetivo verificar se existe uma maior associação entre câncer de mama e a presença do genoma do BLV no tecido mamário amostras dessa população que consome leite cru de animais com altas taxas de BLV infecção. Um estudo molecular de dois genes BLV foi realizado em 88 parênquimas mamários amostras, entre tumores e controles. O fragmento amplificado foi submetido ao sequenciamento proviral de BLV e sua identidade foi confirmada usando GenBank. Os genes provirais do BLV foram amplificado a partir de amostras de parênquima mamário de tumor e amostras de controle de tecido saudável de mulheres, revelando 95,9% (47/49) e 59% (23/39) de positividade, respectivamente. Nossos resultados mostram a maior correlação de BLV e câncer de mama humano encontrado no mundo até o momento na população de Minas Gerais, Brasil.porUniversidade Federal de Minas GeraisUFMGBrasilMED - DEPARTAMENTO DE ANATOMIA PATOLÓGICA E MEDICINA LEGALVET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVAPlos oneMedicinaLeukemia Virus, BovineBreast NeoplasmsBovine Leukemia Virus (BLV)Human breast cancerMinas GeraisBrasilHigh positivity values for bovine leukemia virus in human breast cancer cases from Minas Gerais, BrazilAltos valores de positividade para o vírus da leucemia bovina em casos de câncer de mama humano de Minas Gerais, Brasilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0239745doi:10.1371/journal.pone.0239745Emília DelarmelinaGrazielle Cossenzo Florentino GalinariCristiana Buzelin NunesRômulo Cerqueira LeiteÉrica Azevedo CostaJenner Karlisson Pimenta Dos ReisMarcelo Araújo BuzelinBreno Samuel de SouzaFrancielli Martins SoutoJuliana Marques BicalhoRebeca Jéssica Falcão CâmaraCláudia Fideles ResendeBruna Lopes BuenoRaphael Mattoso Victorapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/41159/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALHigh positivity values for bovine leukemia.pdfHigh positivity values for bovine leukemia.pdfapplication/pdf681820https://repositorio.ufmg.br/bitstream/1843/41159/2/High%20positivity%20values%20for%20bovine%20leukemia.pdf80739ee938ecf3c79ef665e16bf6b17cMD521843/411592022-04-26 16:52:50.618oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-04-26T19:52:50Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
High positivity values for bovine leukemia virus in human breast cancer cases from Minas Gerais, Brazil |
dc.title.alternative.pt_BR.fl_str_mv |
Altos valores de positividade para o vírus da leucemia bovina em casos de câncer de mama humano de Minas Gerais, Brasil |
title |
High positivity values for bovine leukemia virus in human breast cancer cases from Minas Gerais, Brazil |
spellingShingle |
High positivity values for bovine leukemia virus in human breast cancer cases from Minas Gerais, Brazil Emília Delarmelina Bovine Leukemia Virus (BLV) Human breast cancer Minas Gerais Brasil Medicina Leukemia Virus, Bovine Breast Neoplasms |
title_short |
High positivity values for bovine leukemia virus in human breast cancer cases from Minas Gerais, Brazil |
title_full |
High positivity values for bovine leukemia virus in human breast cancer cases from Minas Gerais, Brazil |
title_fullStr |
High positivity values for bovine leukemia virus in human breast cancer cases from Minas Gerais, Brazil |
title_full_unstemmed |
High positivity values for bovine leukemia virus in human breast cancer cases from Minas Gerais, Brazil |
title_sort |
High positivity values for bovine leukemia virus in human breast cancer cases from Minas Gerais, Brazil |
author |
Emília Delarmelina |
author_facet |
Emília Delarmelina Grazielle Cossenzo Florentino Galinari Cristiana Buzelin Nunes Rômulo Cerqueira Leite Érica Azevedo Costa Jenner Karlisson Pimenta Dos Reis Marcelo Araújo Buzelin Breno Samuel de Souza Francielli Martins Souto Juliana Marques Bicalho Rebeca Jéssica Falcão Câmara Cláudia Fideles Resende Bruna Lopes Bueno Raphael Mattoso Victor |
author_role |
author |
author2 |
Grazielle Cossenzo Florentino Galinari Cristiana Buzelin Nunes Rômulo Cerqueira Leite Érica Azevedo Costa Jenner Karlisson Pimenta Dos Reis Marcelo Araújo Buzelin Breno Samuel de Souza Francielli Martins Souto Juliana Marques Bicalho Rebeca Jéssica Falcão Câmara Cláudia Fideles Resende Bruna Lopes Bueno Raphael Mattoso Victor |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Emília Delarmelina Grazielle Cossenzo Florentino Galinari Cristiana Buzelin Nunes Rômulo Cerqueira Leite Érica Azevedo Costa Jenner Karlisson Pimenta Dos Reis Marcelo Araújo Buzelin Breno Samuel de Souza Francielli Martins Souto Juliana Marques Bicalho Rebeca Jéssica Falcão Câmara Cláudia Fideles Resende Bruna Lopes Bueno Raphael Mattoso Victor |
dc.subject.por.fl_str_mv |
Bovine Leukemia Virus (BLV) Human breast cancer Minas Gerais Brasil |
topic |
Bovine Leukemia Virus (BLV) Human breast cancer Minas Gerais Brasil Medicina Leukemia Virus, Bovine Breast Neoplasms |
dc.subject.other.pt_BR.fl_str_mv |
Medicina Leukemia Virus, Bovine Breast Neoplasms |
description |
Bovine leukemia virus (BLV) is a retrovirus that causes lymphoma in cattle worldwide and has also been associated with breast cancer in humans. The mechanism of BLV infection in humans and its implication as a primary cause of cancer in women are not known yet. BLV infection in humans may be caused by the consumption of milk and milk-products or meat from infected animals. Breast cancer incidence rates in Brazil are high, corresponding to 29.5% a year of cancer cases among women. In 2020, an estimated 66,280 new cases of breast cancer are expected, whereas in 2018 breast cancer has led to 17,572 deaths, the highest incidence and lethality among cancers in women in this country that year. BLV infection occurrence ranges from 60 to 95% in dairy herds. In addition, there are some regions, such as the Minas Gerais State, southeastern Brazil, where the population traditionally consume unpasteurized dairy products. Taken together, this study aimed to verify if there is a higher association between breast cancer and the presence of BLV genome in breast tissue samples within this population that consumes raw milk from animals with high rates of BLV infection. A molecular study of two BLV genes was carried out in 88 breast parenchyma samples, between tumors and controls. The amplified fragment was subjected to BLV proviral sequencing and its identity was confirmed using GenBank. BLV proviral genes were amplified from tumor breast parenchyma samples and healthy tissue control samples from women, revealing a 95.9% (47/49) and 59% (23/39) positivity, respectively. Our results show the highest correlation of BLV and human breast cancer found in the world to date within the population of Minas Gerais, Brazil. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020-10-05 |
dc.date.accessioned.fl_str_mv |
2022-04-26T19:52:50Z |
dc.date.available.fl_str_mv |
2022-04-26T19:52:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/41159 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1371/journal.pone.0239745 |
dc.identifier.issn.pt_BR.fl_str_mv |
19326203 |
dc.identifier.orcid.pt_BR.fl_str_mv |
0000-0002-5266-6870 |
identifier_str_mv |
10.1371/journal.pone.0239745 19326203 0000-0002-5266-6870 |
url |
http://hdl.handle.net/1843/41159 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.ispartof.pt_BR.fl_str_mv |
Plos one |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
MED - DEPARTAMENTO DE ANATOMIA PATOLÓGICA E MEDICINA LEGAL VET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVA |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
instacron_str |
UFMG |
institution |
UFMG |
reponame_str |
Repositório Institucional da UFMG |
collection |
Repositório Institucional da UFMG |
bitstream.url.fl_str_mv |
https://repositorio.ufmg.br/bitstream/1843/41159/1/License.txt https://repositorio.ufmg.br/bitstream/1843/41159/2/High%20positivity%20values%20for%20bovine%20leukemia.pdf |
bitstream.checksum.fl_str_mv |
fa505098d172de0bc8864fc1287ffe22 80739ee938ecf3c79ef665e16bf6b17c |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG) |
repository.mail.fl_str_mv |
|
_version_ |
1803589186435940352 |