Cytokine signatures associated with early onset, active lesions and late cicatricial events of retinochoroidal commitment in infants with congenital toxoplasmosis
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://10.1093/infdis/jiw041 http://hdl.handle.net/1843/55291 https://orcid.org/0000-0002-0912-3782 |
Resumo: | Background. Ocular toxoplasmosis is a prominent and severe condition of high incidence in Brazil. The current study provides new insights into the immunological events that can be associated with retinochoroiditis in the setting of congenital toxoplasmosis in human infants. Methods. Flow cytometry of intracytoplasmic cytokines in leukocyte subsets following in vitro short-term antigenic recall in infants with congenital T. gondii infection. Results. Our data demonstrates that whereas neutrophils and monocytes from T. gondii–infected infants display a combination of proinflammatory and regulatory cytokine profiles, natural killer cells showed a predominantly proinflammatory profile upon in vitro T. gondii stimulation. The proinflammatory response of CD4+ and CD8+ T cells, characterized by the production of interferon γ (IFN-γ) and interleukin 17 in patients with an active retinochoroidal lesion, revealed the presence of IFN-γ and tumor necrosis factor α during early and late immunological events. This specific proinflammatory pattern is associated with early events and active retinochoroidal lesion, whereas a robust monocyte-derived interleukin 10–mediated profile is observed in children with cicatricial ocular lesions. Conclusions. These findings support the existence of a progressive immunological environment concomitant with the initial,apical, and cicatricial phases in the process of retinochoroidal lesion formation in infants with congenital toxoplasmosis that may be relevant in the establishment of stage-specific clinical management. |
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2023-06-23T20:19:51Z2023-06-23T20:19:51Z2016-03-052131219621970https://10.1093/infdis/jiw04100221899http://hdl.handle.net/1843/55291https://orcid.org/0000-0002-0912-3782Background. Ocular toxoplasmosis is a prominent and severe condition of high incidence in Brazil. The current study provides new insights into the immunological events that can be associated with retinochoroiditis in the setting of congenital toxoplasmosis in human infants. Methods. Flow cytometry of intracytoplasmic cytokines in leukocyte subsets following in vitro short-term antigenic recall in infants with congenital T. gondii infection. Results. Our data demonstrates that whereas neutrophils and monocytes from T. gondii–infected infants display a combination of proinflammatory and regulatory cytokine profiles, natural killer cells showed a predominantly proinflammatory profile upon in vitro T. gondii stimulation. The proinflammatory response of CD4+ and CD8+ T cells, characterized by the production of interferon γ (IFN-γ) and interleukin 17 in patients with an active retinochoroidal lesion, revealed the presence of IFN-γ and tumor necrosis factor α during early and late immunological events. This specific proinflammatory pattern is associated with early events and active retinochoroidal lesion, whereas a robust monocyte-derived interleukin 10–mediated profile is observed in children with cicatricial ocular lesions. Conclusions. These findings support the existence of a progressive immunological environment concomitant with the initial,apical, and cicatricial phases in the process of retinochoroidal lesion formation in infants with congenital toxoplasmosis that may be relevant in the establishment of stage-specific clinical management.engUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE PARASITOLOGIAMED - DEPARTAMENTO DE CLÍNICA MÉDICAMED - DEPARTAMENTO DE OFTALMOLOGIA E OTORRINOLARINGOLOGIAThe Journal of Infectious DiseasesToxoplasmose CongênitaCriançasCoriorretinopatia de BirdshotCongenital toxoplasmosisCytokine profileRetinochoroiditisInfantsActive and cicatricial lesionsCytokine signatures associated with early onset, active lesions and late cicatricial events of retinochoroidal commitment in infants with congenital toxoplasmosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://academic.oup.com/jid/article/213/12/1962/2572144Ana Carolina Aguiar Vasconcelos CarneiroRicardo Wagner Almeida VitorOlindo Assis Martins-filhoUfmg Congenital Toxoplasmosis Group Brazilian 'ufmg-ctbgJúlia Gatti Ladeia CostaAnderson da Silva MachadoGláucia Manzan Queiroz AndradeSamantha Ribeiro BélaJordana g. Coelho-dos-reisEloísa A.v. FerroJosé Nélio JanuárioAndréa Teixeira-carvalhoDaniel Vitor Vasconcelos-santosapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/55291/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALCytokine Signatures Associated with Early Onset, Active Lesions and late cicatrical events pdfa.pdfCytokine Signatures Associated with Early Onset, Active Lesions and late cicatrical events pdfa.pdfapplication/pdf720523https://repositorio.ufmg.br/bitstream/1843/55291/2/Cytokine%20Signatures%20Associated%20with%20Early%20Onset%2c%20Active%20Lesions%20and%20late%20cicatrical%20events%20pdfa.pdf16188e8ae5339d2451e7332538da769dMD521843/552912023-06-23 17:34:11.763oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-06-23T20:34:11Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Cytokine signatures associated with early onset, active lesions and late cicatricial events of retinochoroidal commitment in infants with congenital toxoplasmosis |
title |
Cytokine signatures associated with early onset, active lesions and late cicatricial events of retinochoroidal commitment in infants with congenital toxoplasmosis |
spellingShingle |
Cytokine signatures associated with early onset, active lesions and late cicatricial events of retinochoroidal commitment in infants with congenital toxoplasmosis Ana Carolina Aguiar Vasconcelos Carneiro Congenital toxoplasmosis Cytokine profile Retinochoroiditis Infants Active and cicatricial lesions Toxoplasmose Congênita Crianças Coriorretinopatia de Birdshot |
title_short |
Cytokine signatures associated with early onset, active lesions and late cicatricial events of retinochoroidal commitment in infants with congenital toxoplasmosis |
title_full |
Cytokine signatures associated with early onset, active lesions and late cicatricial events of retinochoroidal commitment in infants with congenital toxoplasmosis |
title_fullStr |
Cytokine signatures associated with early onset, active lesions and late cicatricial events of retinochoroidal commitment in infants with congenital toxoplasmosis |
title_full_unstemmed |
Cytokine signatures associated with early onset, active lesions and late cicatricial events of retinochoroidal commitment in infants with congenital toxoplasmosis |
title_sort |
Cytokine signatures associated with early onset, active lesions and late cicatricial events of retinochoroidal commitment in infants with congenital toxoplasmosis |
author |
Ana Carolina Aguiar Vasconcelos Carneiro |
author_facet |
Ana Carolina Aguiar Vasconcelos Carneiro Ricardo Wagner Almeida Vitor Olindo Assis Martins-filho Ufmg Congenital Toxoplasmosis Group Brazilian 'ufmg-ctbg Júlia Gatti Ladeia Costa Anderson da Silva Machado Gláucia Manzan Queiroz Andrade Samantha Ribeiro Béla Jordana g. Coelho-dos-reis Eloísa A.v. Ferro José Nélio Januário Andréa Teixeira-carvalho Daniel Vitor Vasconcelos-santos |
author_role |
author |
author2 |
Ricardo Wagner Almeida Vitor Olindo Assis Martins-filho Ufmg Congenital Toxoplasmosis Group Brazilian 'ufmg-ctbg Júlia Gatti Ladeia Costa Anderson da Silva Machado Gláucia Manzan Queiroz Andrade Samantha Ribeiro Béla Jordana g. Coelho-dos-reis Eloísa A.v. Ferro José Nélio Januário Andréa Teixeira-carvalho Daniel Vitor Vasconcelos-santos |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ana Carolina Aguiar Vasconcelos Carneiro Ricardo Wagner Almeida Vitor Olindo Assis Martins-filho Ufmg Congenital Toxoplasmosis Group Brazilian 'ufmg-ctbg Júlia Gatti Ladeia Costa Anderson da Silva Machado Gláucia Manzan Queiroz Andrade Samantha Ribeiro Béla Jordana g. Coelho-dos-reis Eloísa A.v. Ferro José Nélio Januário Andréa Teixeira-carvalho Daniel Vitor Vasconcelos-santos |
dc.subject.por.fl_str_mv |
Congenital toxoplasmosis Cytokine profile Retinochoroiditis Infants Active and cicatricial lesions |
topic |
Congenital toxoplasmosis Cytokine profile Retinochoroiditis Infants Active and cicatricial lesions Toxoplasmose Congênita Crianças Coriorretinopatia de Birdshot |
dc.subject.other.pt_BR.fl_str_mv |
Toxoplasmose Congênita Crianças Coriorretinopatia de Birdshot |
description |
Background. Ocular toxoplasmosis is a prominent and severe condition of high incidence in Brazil. The current study provides new insights into the immunological events that can be associated with retinochoroiditis in the setting of congenital toxoplasmosis in human infants. Methods. Flow cytometry of intracytoplasmic cytokines in leukocyte subsets following in vitro short-term antigenic recall in infants with congenital T. gondii infection. Results. Our data demonstrates that whereas neutrophils and monocytes from T. gondii–infected infants display a combination of proinflammatory and regulatory cytokine profiles, natural killer cells showed a predominantly proinflammatory profile upon in vitro T. gondii stimulation. The proinflammatory response of CD4+ and CD8+ T cells, characterized by the production of interferon γ (IFN-γ) and interleukin 17 in patients with an active retinochoroidal lesion, revealed the presence of IFN-γ and tumor necrosis factor α during early and late immunological events. This specific proinflammatory pattern is associated with early events and active retinochoroidal lesion, whereas a robust monocyte-derived interleukin 10–mediated profile is observed in children with cicatricial ocular lesions. Conclusions. These findings support the existence of a progressive immunological environment concomitant with the initial,apical, and cicatricial phases in the process of retinochoroidal lesion formation in infants with congenital toxoplasmosis that may be relevant in the establishment of stage-specific clinical management. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-03-05 |
dc.date.accessioned.fl_str_mv |
2023-06-23T20:19:51Z |
dc.date.available.fl_str_mv |
2023-06-23T20:19:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/55291 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://10.1093/infdis/jiw041 |
dc.identifier.issn.pt_BR.fl_str_mv |
00221899 |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0002-0912-3782 |
url |
https://10.1093/infdis/jiw041 http://hdl.handle.net/1843/55291 https://orcid.org/0000-0002-0912-3782 |
identifier_str_mv |
00221899 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
The Journal of Infectious Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
ICB - DEPARTAMENTO DE PARASITOLOGIA MED - DEPARTAMENTO DE CLÍNICA MÉDICA MED - DEPARTAMENTO DE OFTALMOLOGIA E OTORRINOLARINGOLOGIA |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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Universidade Federal de Minas Gerais (UFMG) |
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UFMG |
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UFMG |
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