Diversity, distribution and ecology of fungal communities present in Antarctic lake sediments uncovered by DNA metabarcoding
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.1038/s41598-022-12290-6 http://hdl.handle.net/1843/59869 https://orcid.org/0000-0002-1019-8407 https://orcid.org/0000-0002-4565-9103 https://orcid.org/0000-0001-8497-9903 https://orcid.org/0000-0002-2389-3804 https://orcid.org/0000-0002-1450-7609 https://orcid.org/0000-0001-9749-5182 |
Resumo: | We assessed fungal diversity in sediments obtained from four lakes in the South Shetland Islands and James Ross Island, Antarctica, using DNA metabarcoding. We detected 218 amplicon sequence variants (ASVs) dominated by the phyla Ascomycota, Basidiomycota, Mortierellomycota, Mucoromycota and Chytridiomycota. In addition, the rare phyla Aphelidiomycota, Basidiobolomycota, Blastocladiomycota, Monoblepharomycota, Rozellomycota and Zoopagomycota as well as fungal-like Straminopila belonging to the phyla Bacillariophyta and Oomycota were detected. The fungal assemblages were dominated by unknown fungal taxa (Fungal sp. 1 and Fungal sp. 2), followed by Talaromyces rubicundus and Dactylonectria anthuriicola. In general, they displayed high diversity, richness and moderate dominance. Sequences representing saprophytic, pathogenic and symbiotic fungi were detected, including the phytopathogenic fungus D. anthuriicola that was abundant, in the relatively young Soto Lake on Deception Island. The lake sediments studied contained the DNA of rich, diverse and complex fungal communities, including both fungi commonly reported in Antarctica and other taxa considered to be rare. However, as the study was based on the use of environmental DNA, which does not unequivocally confirm the presence of active or viable organisms, further studies using other approaches such as shotgun sequencing are required to elucidate the ecology of fungi in these Antarctic lake sediments. |
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2023-10-23T21:14:29Z2023-10-23T21:14:29Z202212https://doi.org/10.1038/s41598-022-12290-62045-2322http://hdl.handle.net/1843/59869https://orcid.org/0000-0002-1019-8407https://orcid.org/0000-0002-4565-9103https://orcid.org/0000-0001-8497-9903https://orcid.org/0000-0002-2389-3804https://orcid.org/0000-0002-1450-7609https://orcid.org/0000-0001-9749-5182We assessed fungal diversity in sediments obtained from four lakes in the South Shetland Islands and James Ross Island, Antarctica, using DNA metabarcoding. We detected 218 amplicon sequence variants (ASVs) dominated by the phyla Ascomycota, Basidiomycota, Mortierellomycota, Mucoromycota and Chytridiomycota. In addition, the rare phyla Aphelidiomycota, Basidiobolomycota, Blastocladiomycota, Monoblepharomycota, Rozellomycota and Zoopagomycota as well as fungal-like Straminopila belonging to the phyla Bacillariophyta and Oomycota were detected. The fungal assemblages were dominated by unknown fungal taxa (Fungal sp. 1 and Fungal sp. 2), followed by Talaromyces rubicundus and Dactylonectria anthuriicola. In general, they displayed high diversity, richness and moderate dominance. Sequences representing saprophytic, pathogenic and symbiotic fungi were detected, including the phytopathogenic fungus D. anthuriicola that was abundant, in the relatively young Soto Lake on Deception Island. The lake sediments studied contained the DNA of rich, diverse and complex fungal communities, including both fungi commonly reported in Antarctica and other taxa considered to be rare. However, as the study was based on the use of environmental DNA, which does not unequivocally confirm the presence of active or viable organisms, further studies using other approaches such as shotgun sequencing are required to elucidate the ecology of fungi in these Antarctic lake sediments.Avaliamos a diversidade fúngica em sedimentos obtidos de quatro lagos nas Ilhas Shetland do Sul e na Ilha James Ross, na Antártica, usando metabarcoding de DNA. Detectamos 218 variantes de sequências de amplicon (ASVs) dominadas pelos filos Ascomycota, Basidiomycota, Mortierellomycota, Mucoromycota e Chytridiomycota. Além disso, foram detectados os raros filos Aphelidiomycota, Basidiobolomycota, Blastocladiomycota, Monoblepharomycota, Rozellomycota e Zoopagomycota, bem como Straminopila semelhante a fungos pertencentes aos filos Bacillariophyta e Oomycota. As assembleias fúngicas foram dominadas por táxons fúngicos desconhecidos (Fungal sp. 1 e Fungal sp. 2), seguidos por Talaromyces rubicundus e Dactylonectria anthuriicola. Em geral, apresentaram elevada diversidade, riqueza e dominância moderada. Foram detectadas sequências representando fungos saprófitos, patogênicos e simbióticos, incluindo o fungo fitopatogênico D. anthuriicola que era abundante no relativamente jovem Lago Soto na Ilha Deception. Os sedimentos lacustres estudados continham o DNA de comunidades fúngicas ricas, diversas e complexas, incluindo fungos comumente relatados na Antártica e outros táxons considerados raros. No entanto, como o estudo foi baseado no uso de DNA ambiental, que não confirma inequivocamente a presença de organismos ativos ou viáveis, são necessários mais estudos utilizando outras abordagens, como o sequenciamento shotgun, para elucidar a ecologia dos fungos nesses sedimentos lacustres antárticos.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorINCT – Instituto nacional de ciência e tecnologia (Antigo Instituto do Milênio)engUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE BOTÂNICAICB - DEPARTAMENTO DE MICROBIOLOGIAIGC - DEPARTAMENTO DE GEOGRAFIAScientific ReportsMicobiomaEcologia - AntárticaBarcode de DNAFungal communitiesAntarcticDNA metabarcodingDiversity, distribution and ecology of fungal communities present in Antarctic lake sediments uncovered by DNA metabarcodingDiversidade, distribuição e ecologia de comunidades fúngicas presentes em sedimentos de lagos antárticos descobertos por metabarcodificação de DNAinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.nature.com/articles/s41598-022-12290-6Láuren Machado Drumond de SouzaJuan Manuel LirioSilvia Herminda CoriaFabyano Alvares Cardoso LopesPeter ConveyMicheline Carvalho-SilvaFábio Soares de OliveiraCarlos Augusto RosaPaulo Eduardo Aguiar Saraiva CâmaraLuiz Henrique Rosaapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/59869/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALDiversity, distribution and ecology of fungal communities present in Antarctic lake sediments uncovered by DNA metabarcoding.pdfDiversity, distribution and ecology of fungal communities present in Antarctic lake sediments uncovered by DNA metabarcoding.pdfapplication/pdf616109https://repositorio.ufmg.br/bitstream/1843/59869/2/Diversity%2c%20distribution%20and%20ecology%20of%20fungal%20communities%20present%20in%20Antarctic%20lake%20sediments%20uncovered%20by%20DNA%20metabarcoding.pdff13453d6ed977a3ea587124035c1e53cMD521843/598692023-10-23 18:14:29.308oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-10-23T21:14:29Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Diversity, distribution and ecology of fungal communities present in Antarctic lake sediments uncovered by DNA metabarcoding |
dc.title.alternative.pt_BR.fl_str_mv |
Diversidade, distribuição e ecologia de comunidades fúngicas presentes em sedimentos de lagos antárticos descobertos por metabarcodificação de DNA |
title |
Diversity, distribution and ecology of fungal communities present in Antarctic lake sediments uncovered by DNA metabarcoding |
spellingShingle |
Diversity, distribution and ecology of fungal communities present in Antarctic lake sediments uncovered by DNA metabarcoding Láuren Machado Drumond de Souza Fungal communities Antarctic DNA metabarcoding Micobioma Ecologia - Antártica Barcode de DNA |
title_short |
Diversity, distribution and ecology of fungal communities present in Antarctic lake sediments uncovered by DNA metabarcoding |
title_full |
Diversity, distribution and ecology of fungal communities present in Antarctic lake sediments uncovered by DNA metabarcoding |
title_fullStr |
Diversity, distribution and ecology of fungal communities present in Antarctic lake sediments uncovered by DNA metabarcoding |
title_full_unstemmed |
Diversity, distribution and ecology of fungal communities present in Antarctic lake sediments uncovered by DNA metabarcoding |
title_sort |
Diversity, distribution and ecology of fungal communities present in Antarctic lake sediments uncovered by DNA metabarcoding |
author |
Láuren Machado Drumond de Souza |
author_facet |
Láuren Machado Drumond de Souza Juan Manuel Lirio Silvia Herminda Coria Fabyano Alvares Cardoso Lopes Peter Convey Micheline Carvalho-Silva Fábio Soares de Oliveira Carlos Augusto Rosa Paulo Eduardo Aguiar Saraiva Câmara Luiz Henrique Rosa |
author_role |
author |
author2 |
Juan Manuel Lirio Silvia Herminda Coria Fabyano Alvares Cardoso Lopes Peter Convey Micheline Carvalho-Silva Fábio Soares de Oliveira Carlos Augusto Rosa Paulo Eduardo Aguiar Saraiva Câmara Luiz Henrique Rosa |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Láuren Machado Drumond de Souza Juan Manuel Lirio Silvia Herminda Coria Fabyano Alvares Cardoso Lopes Peter Convey Micheline Carvalho-Silva Fábio Soares de Oliveira Carlos Augusto Rosa Paulo Eduardo Aguiar Saraiva Câmara Luiz Henrique Rosa |
dc.subject.por.fl_str_mv |
Fungal communities Antarctic DNA metabarcoding |
topic |
Fungal communities Antarctic DNA metabarcoding Micobioma Ecologia - Antártica Barcode de DNA |
dc.subject.other.pt_BR.fl_str_mv |
Micobioma Ecologia - Antártica Barcode de DNA |
description |
We assessed fungal diversity in sediments obtained from four lakes in the South Shetland Islands and James Ross Island, Antarctica, using DNA metabarcoding. We detected 218 amplicon sequence variants (ASVs) dominated by the phyla Ascomycota, Basidiomycota, Mortierellomycota, Mucoromycota and Chytridiomycota. In addition, the rare phyla Aphelidiomycota, Basidiobolomycota, Blastocladiomycota, Monoblepharomycota, Rozellomycota and Zoopagomycota as well as fungal-like Straminopila belonging to the phyla Bacillariophyta and Oomycota were detected. The fungal assemblages were dominated by unknown fungal taxa (Fungal sp. 1 and Fungal sp. 2), followed by Talaromyces rubicundus and Dactylonectria anthuriicola. In general, they displayed high diversity, richness and moderate dominance. Sequences representing saprophytic, pathogenic and symbiotic fungi were detected, including the phytopathogenic fungus D. anthuriicola that was abundant, in the relatively young Soto Lake on Deception Island. The lake sediments studied contained the DNA of rich, diverse and complex fungal communities, including both fungi commonly reported in Antarctica and other taxa considered to be rare. However, as the study was based on the use of environmental DNA, which does not unequivocally confirm the presence of active or viable organisms, further studies using other approaches such as shotgun sequencing are required to elucidate the ecology of fungi in these Antarctic lake sediments. |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022 |
dc.date.accessioned.fl_str_mv |
2023-10-23T21:14:29Z |
dc.date.available.fl_str_mv |
2023-10-23T21:14:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/59869 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1038/s41598-022-12290-6 |
dc.identifier.issn.pt_BR.fl_str_mv |
2045-2322 |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0002-1019-8407 https://orcid.org/0000-0002-4565-9103 https://orcid.org/0000-0001-8497-9903 https://orcid.org/0000-0002-2389-3804 https://orcid.org/0000-0002-1450-7609 https://orcid.org/0000-0001-9749-5182 |
url |
https://doi.org/10.1038/s41598-022-12290-6 http://hdl.handle.net/1843/59869 https://orcid.org/0000-0002-1019-8407 https://orcid.org/0000-0002-4565-9103 https://orcid.org/0000-0001-8497-9903 https://orcid.org/0000-0002-2389-3804 https://orcid.org/0000-0002-1450-7609 https://orcid.org/0000-0001-9749-5182 |
identifier_str_mv |
2045-2322 |
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eng |
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eng |
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Scientific Reports |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de Minas Gerais |
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UFMG |
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Brasil |
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ICB - DEPARTAMENTO DE BOTÂNICA ICB - DEPARTAMENTO DE MICROBIOLOGIA IGC - DEPARTAMENTO DE GEOGRAFIA |
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Universidade Federal de Minas Gerais |
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