Radiolabeling of cidofovir with technetium-99m and biodistribution studies
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.1590/s2175-97902019000418511 http://hdl.handle.net/1843/47146 |
Resumo: | Radiolabeling cidofovir with technetium-99m (99mTc-CDV) is an innovative procedure that enables real-time monitoring of the drug. Essays were performed in vitro, showing high radiolabel stability within 24 h. Blood clearance, biodistribution studies, and scintigraphic images were performed in healthy mice in order to evaluate the profile of the drug in vivo. 99mTc-CDV showed biphasic blood circulation time and significant kidney uptake, indicating that 99mTc-CDV is preferentially eliminated by the renal route. Bones also showed important uptake throughout the experiment. In summary, cidofovir was successfully labeled with technetium-99m and might be used in further studies to track the drug. |
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2022-11-10T20:10:03Z2022-11-10T20:10:03Z2020-12-0956e1851119https://doi.org/10.1590/s2175-979020190004185112175-9790http://hdl.handle.net/1843/47146Radiolabeling cidofovir with technetium-99m (99mTc-CDV) is an innovative procedure that enables real-time monitoring of the drug. Essays were performed in vitro, showing high radiolabel stability within 24 h. Blood clearance, biodistribution studies, and scintigraphic images were performed in healthy mice in order to evaluate the profile of the drug in vivo. 99mTc-CDV showed biphasic blood circulation time and significant kidney uptake, indicating that 99mTc-CDV is preferentially eliminated by the renal route. Bones also showed important uptake throughout the experiment. In summary, cidofovir was successfully labeled with technetium-99m and might be used in further studies to track the drug.A radiomarcação do cidofovir com tecnécio-99m (99mTc-CDV) é um procedimento inovador que permite o monitoramento em tempo real do medicamento. Os ensaios foram realizados in vitro, mostrando alta estabilidade do radiomarcador em 24 h. A depuração sanguínea, estudos de biodistribuição e imagens cintilográficas foram realizados em camundongos saudáveis para avaliar o perfil da droga in vivo. O 99mTc-CDV apresentou tempo de circulação sanguínea bifásico e captação renal significativa, indicando que o 99mTc-CDV é eliminado preferencialmente pela via renal. Bones também mostrou absorção importante ao longo do experimento. Em resumo, o cidofovir foi marcado com sucesso com tecnécio-99m e pode ser usado em estudos posteriores para rastrear a droga.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoengUniversidade Federal de Minas GeraisUFMGBrasilFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICASFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOSBrazilian Journal of Pharmaceutical SciencesCidofovirTecnécioCintilografiaCidofovirRadiotracerTechnetium-99mBiodistributionScintigraphic imagingRadiolabeling of cidofovir with technetium-99m and biodistribution studiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.revistas.usp.br/bjps/article/view/181834Raquel Gregorio ArribadaNara Caroline PereiraValbert Nascimento CardosoArmando da Silva Cunha JúniorAndré Luís Branco de Barrosapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/47146/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALRadiolabeling of cidofovir with technetium-99m and biodistribution studies.pdfRadiolabeling of cidofovir with technetium-99m and biodistribution studies.pdfapplication/pdf736274https://repositorio.ufmg.br/bitstream/1843/47146/2/Radiolabeling%20of%20cidofovir%20with%20technetium-99m%20and%20biodistribution%20studies.pdf8a57428b33dfff3e395888d001e66b5aMD521843/471462022-11-10 17:10:03.458oai:repositorio.ufmg.br:1843/47146TElDRU7vv71BIERFIERJU1RSSUJVSe+/ve+/vU8gTu+/vU8tRVhDTFVTSVZBIERPIFJFUE9TSVTvv71SSU8gSU5TVElUVUNJT05BTCBEQSBVRk1HCiAKCkNvbSBhIGFwcmVzZW50Ye+/ve+/vW8gZGVzdGEgbGljZW7vv71hLCB2b2Pvv70gKG8gYXV0b3IgKGVzKSBvdSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGRlIGF1dG9yKSBjb25jZWRlIGFvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIChSSS1VRk1HKSBvIGRpcmVpdG8gbu+/vW8gZXhjbHVzaXZvIGUgaXJyZXZvZ++/vXZlbCBkZSByZXByb2R1emlyIGUvb3UgZGlzdHJpYnVpciBhIHN1YSBwdWJsaWNh77+977+9byAoaW5jbHVpbmRvIG8gcmVzdW1vKSBwb3IgdG9kbyBvIG11bmRvIG5vIGZvcm1hdG8gaW1wcmVzc28gZSBlbGV0cu+/vW5pY28gZSBlbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mg77+9dWRpbyBvdSB277+9ZGVvLgoKVm9j77+9IGRlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zvv710aWNhIGRlIGNvcHlyaWdodCBkYSBlZGl0b3JhIGRvIHNldSBkb2N1bWVudG8gZSBxdWUgY29uaGVjZSBlIGFjZWl0YSBhcyBEaXJldHJpemVzIGRvIFJJLVVGTUcuCgpWb2Pvv70gY29uY29yZGEgcXVlIG8gUmVwb3NpdO+/vXJpbyBJbnN0aXR1Y2lvbmFsIGRhIFVGTUcgcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250Ze+/vWRvLCB0cmFuc3BvciBhIHN1YSBwdWJsaWNh77+977+9byBwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHvv73vv71vLgoKVm9j77+9IHRhbWLvv71tIGNvbmNvcmRhIHF1ZSBvIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRk1HIHBvZGUgbWFudGVyIG1haXMgZGUgdW1hIGPvv71waWEgZGUgc3VhIHB1YmxpY2Hvv73vv71vIHBhcmEgZmlucyBkZSBzZWd1cmFu77+9YSwgYmFjay11cCBlIHByZXNlcnZh77+977+9by4KClZvY++/vSBkZWNsYXJhIHF1ZSBhIHN1YSBwdWJsaWNh77+977+9byDvv70gb3JpZ2luYWwgZSBxdWUgdm9j77+9IHRlbSBvIHBvZGVyIGRlIGNvbmNlZGVyIG9zIGRpcmVpdG9zIGNvbnRpZG9zIG5lc3RhIGxpY2Vu77+9YS4gVm9j77+9IHRhbWLvv71tIGRlY2xhcmEgcXVlIG8gZGVw77+9c2l0byBkZSBzdWEgcHVibGljYe+/ve+/vW8gbu+/vW8sIHF1ZSBzZWphIGRlIHNldSBjb25oZWNpbWVudG8sIGluZnJpbmdlIGRpcmVpdG9zIGF1dG9yYWlzIGRlIG5pbmd177+9bS4KCkNhc28gYSBzdWEgcHVibGljYe+/ve+/vW8gY29udGVuaGEgbWF0ZXJpYWwgcXVlIHZvY++/vSBu77+9byBwb3NzdWkgYSB0aXR1bGFyaWRhZGUgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCB2b2Pvv70gZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc++/vW8gaXJyZXN0cml0YSBkbyBkZXRlbnRvciBkb3MgZGlyZWl0b3MgYXV0b3JhaXMgcGFyYSBjb25jZWRlciBhbyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBvcyBkaXJlaXRvcyBhcHJlc2VudGFkb3MgbmVzdGEgbGljZW7vv71hLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3Tvv70gY2xhcmFtZW50ZSBpZGVudGlmaWNhZG8gZSByZWNvbmhlY2lkbyBubyB0ZXh0byBvdSBubyBjb250Ze+/vWRvIGRhIHB1YmxpY2Hvv73vv71vIG9yYSBkZXBvc2l0YWRhLgoKQ0FTTyBBIFBVQkxJQ0Hvv73vv71PIE9SQSBERVBPU0lUQURBIFRFTkhBIFNJRE8gUkVTVUxUQURPIERFIFVNIFBBVFJPQ++/vU5JTyBPVSBBUE9JTyBERSBVTUEgQUfvv71OQ0lBIERFIEZPTUVOVE8gT1UgT1VUUk8gT1JHQU5JU01PLCBWT0Pvv70gREVDTEFSQSBRVUUgUkVTUEVJVE9VIFRPRE9TIEUgUVVBSVNRVUVSIERJUkVJVE9TIERFIFJFVklT77+9TyBDT01PIFRBTULvv71NIEFTIERFTUFJUyBPQlJJR0Hvv73vv71FUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKTyBSZXBvc2l077+9cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZNRyBzZSBjb21wcm9tZXRlIGEgaWRlbnRpZmljYXIgY2xhcmFtZW50ZSBvIHNldSBub21lKHMpIG91IG8ocykgbm9tZXMocykgZG8ocykgZGV0ZW50b3IoZXMpIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBkYSBwdWJsaWNh77+977+9bywgZSBu77+9byBmYXLvv70gcXVhbHF1ZXIgYWx0ZXJh77+977+9bywgYWzvv71tIGRhcXVlbGFzIGNvbmNlZGlkYXMgcG9yIGVzdGEgbGljZW7vv71hLgo=Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-11-10T20:10:03Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Radiolabeling of cidofovir with technetium-99m and biodistribution studies |
title |
Radiolabeling of cidofovir with technetium-99m and biodistribution studies |
spellingShingle |
Radiolabeling of cidofovir with technetium-99m and biodistribution studies Raquel Gregorio Arribada Cidofovir Radiotracer Technetium-99m Biodistribution Scintigraphic imaging Cidofovir Tecnécio Cintilografia |
title_short |
Radiolabeling of cidofovir with technetium-99m and biodistribution studies |
title_full |
Radiolabeling of cidofovir with technetium-99m and biodistribution studies |
title_fullStr |
Radiolabeling of cidofovir with technetium-99m and biodistribution studies |
title_full_unstemmed |
Radiolabeling of cidofovir with technetium-99m and biodistribution studies |
title_sort |
Radiolabeling of cidofovir with technetium-99m and biodistribution studies |
author |
Raquel Gregorio Arribada |
author_facet |
Raquel Gregorio Arribada Nara Caroline Pereira Valbert Nascimento Cardoso Armando da Silva Cunha Júnior André Luís Branco de Barros |
author_role |
author |
author2 |
Nara Caroline Pereira Valbert Nascimento Cardoso Armando da Silva Cunha Júnior André Luís Branco de Barros |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Raquel Gregorio Arribada Nara Caroline Pereira Valbert Nascimento Cardoso Armando da Silva Cunha Júnior André Luís Branco de Barros |
dc.subject.por.fl_str_mv |
Cidofovir Radiotracer Technetium-99m Biodistribution Scintigraphic imaging |
topic |
Cidofovir Radiotracer Technetium-99m Biodistribution Scintigraphic imaging Cidofovir Tecnécio Cintilografia |
dc.subject.other.pt_BR.fl_str_mv |
Cidofovir Tecnécio Cintilografia |
description |
Radiolabeling cidofovir with technetium-99m (99mTc-CDV) is an innovative procedure that enables real-time monitoring of the drug. Essays were performed in vitro, showing high radiolabel stability within 24 h. Blood clearance, biodistribution studies, and scintigraphic images were performed in healthy mice in order to evaluate the profile of the drug in vivo. 99mTc-CDV showed biphasic blood circulation time and significant kidney uptake, indicating that 99mTc-CDV is preferentially eliminated by the renal route. Bones also showed important uptake throughout the experiment. In summary, cidofovir was successfully labeled with technetium-99m and might be used in further studies to track the drug. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020-12-09 |
dc.date.accessioned.fl_str_mv |
2022-11-10T20:10:03Z |
dc.date.available.fl_str_mv |
2022-11-10T20:10:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/47146 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1590/s2175-97902019000418511 |
dc.identifier.issn.pt_BR.fl_str_mv |
2175-9790 |
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https://doi.org/10.1590/s2175-97902019000418511 http://hdl.handle.net/1843/47146 |
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2175-9790 |
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eng |
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eng |
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Brazilian Journal of Pharmaceutical Sciences |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
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Brasil |
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FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS FAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS |
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Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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Universidade Federal de Minas Gerais (UFMG) |
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UFMG |
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UFMG |
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