Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.1590/1414-431X20177033 http://hdl.handle.net/1843/56289 https://orcid.org/0000-0001-9550-6095 https://orcid.org/0000-0003-0167-4093 https://orcid.org/0000-0003-2318-3340 https://orcid.org/0000-0003-2823-4530 https://orcid.org/0000-0003-2116-6379 https://orcid.org/0000-0002-5363-8329 |
Resumo: | In the present study, we successfully demonstrated for the first time the existence of cardiac proteomic differences between non-selectively bred rats with distinct intrinsic exercise capacities. A proteomic approach based on two-dimensional gel electrophoresis coupled to mass spectrometry was used to study the left ventricle (LV) tissue proteome of rats with distinct intrinsic exercise capacity. Low running performance (LRP) and high running performance (HRP) rats were categorized by a treadmill exercise test, according to distance run to exhaustion. The running capacity of HRPs was 3.5-fold greater than LRPs. Protein profiling revealed 29 differences between HRP and LRP rats (15 proteins were identified). We detected alterations in components involved in metabolism, antioxidant and stress response, microfibrillar and cytoskeletal proteins. Contractile proteins were upregulated in the LVs of HRP rats (a-myosin heavy chain-6, myosin light chain-1 and creatine kinase), whereas the LVs of LRP rats exhibited upregulation in proteins associated with stress response (aldehyde dehydrogenase 2, a-crystallin B chain and HSPb-2). In addition, the cytoskeletal proteins desmin and a-actin were upregulated in LRPs. Taken together, our results suggest that the increased contractile protein levels in HRP rats partly accounted for their improved exercise capacity, and that proteins considered risk factors to the development of cardiovascular disease were expressed in higher amounts in LRP animals. |
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2023-07-14T20:26:34Z2023-07-14T20:26:34Z2018513https://doi.org/10.1590/1414-431X201770331414-431Xhttp://hdl.handle.net/1843/56289https://orcid.org/0000-0001-9550-6095https://orcid.org/0000-0003-0167-4093https://orcid.org/0000-0003-2318-3340https://orcid.org/0000-0003-2823-4530https://orcid.org/0000-0003-2116-6379https://orcid.org/0000-0002-5363-8329In the present study, we successfully demonstrated for the first time the existence of cardiac proteomic differences between non-selectively bred rats with distinct intrinsic exercise capacities. A proteomic approach based on two-dimensional gel electrophoresis coupled to mass spectrometry was used to study the left ventricle (LV) tissue proteome of rats with distinct intrinsic exercise capacity. Low running performance (LRP) and high running performance (HRP) rats were categorized by a treadmill exercise test, according to distance run to exhaustion. The running capacity of HRPs was 3.5-fold greater than LRPs. Protein profiling revealed 29 differences between HRP and LRP rats (15 proteins were identified). We detected alterations in components involved in metabolism, antioxidant and stress response, microfibrillar and cytoskeletal proteins. Contractile proteins were upregulated in the LVs of HRP rats (a-myosin heavy chain-6, myosin light chain-1 and creatine kinase), whereas the LVs of LRP rats exhibited upregulation in proteins associated with stress response (aldehyde dehydrogenase 2, a-crystallin B chain and HSPb-2). In addition, the cytoskeletal proteins desmin and a-actin were upregulated in LRPs. Taken together, our results suggest that the increased contractile protein levels in HRP rats partly accounted for their improved exercise capacity, and that proteins considered risk factors to the development of cardiovascular disease were expressed in higher amounts in LRP animals.No presente estudo, demonstramos pela primeira vez com sucesso a existência de diferenças proteômicas cardíacas entre ratos criados de forma não seletiva com capacidades de exercício intrínsecas distintas. Uma abordagem proteômica baseada em eletroforese bidimensional em gel acoplada à espectrometria de massa foi utilizada para estudar o proteoma do tecido do ventrículo esquerdo (VE) de ratos com capacidade de exercício intrínseca distinta. Ratos de baixo desempenho na corrida (LRP) e alto desempenho na corrida (HRP) foram categorizados por um teste de exercício em esteira, de acordo com a distância percorrida até a exaustão. A capacidade de funcionamento dos HRPs foi 3,5 vezes maior do que os LRPs. O perfil de proteína revelou 29 diferenças entre ratos HRP e LRP (15 proteínas foram identificadas). Detectamos alterações em componentes envolvidos no metabolismo, resposta antioxidante e ao estresse, proteínas microfibrilares e do citoesqueleto. As proteínas contráteis foram reguladas positivamente nos LVs de ratos HRP (a-miosina cadeia pesada-6, cadeia leve de miosina-1 e creatina quinase), enquanto os LVs de ratos LRP exibiram regulação positiva em proteínas associadas à resposta ao estresse (aldeído desidrogenase 2, a- cadeia B cristalina e HSPb-2). Além disso, as proteínas do citoesqueleto desmina e a-actina foram reguladas positivamente em LRPs. Em conjunto, nossos resultados sugerem que os níveis aumentados de proteína contrátil em ratos HRP foram parcialmente responsáveis por sua capacidade de exercício melhorada, e que as proteínas consideradas fatores de risco para o desenvolvimento de doenças cardiovasculares foram expressas em maiores quantidades em animais LRP.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoOutra AgênciaengUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE PARASITOLOGIABrazilian Journal of Medical and Biological ResearchProteômicaRatosExercício físicoEspectrometria de massasProteínasInborn aerobic capacityProteomics2-DEMSHeart proteinsCardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred ratsPadrões de expressão de proteínas cardíacas estão associados com capacidade de exercício inata distinta em ratos criados não seletivamenteinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.scielo.br/j/bjmbr/a/xqSWX6nMnFzKSrDXgXRGnsG/?lang=en#Leonardo Perin RibeiroLeandro Ceotto Freitas LimaGustavo Baptista NaumannSilvana dos Santos MeyrellesWellington LunzSimone da Fonseca PiresHélida Monteiro de AndradeJuliana Brambilla Carnielli TrindadeSuely Gomes de Figueiredoapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/56289/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALCardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats.pdfCardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats.pdfapplication/pdf10157265https://repositorio.ufmg.br/bitstream/1843/56289/2/Cardiac%20protein%20expression%20patterns%20are%20associated%20with%20distinct%20inborn%20exercise%20capacity%20in%20non-selectively%20bred%20rats.pdfb8c096d01a29eb188bfb8ce88dae39c6MD521843/562892023-07-14 17:26:35.02oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-07-14T20:26:35Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats |
dc.title.alternative.pt_BR.fl_str_mv |
Padrões de expressão de proteínas cardíacas estão associados com capacidade de exercício inata distinta em ratos criados não seletivamente |
title |
Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats |
spellingShingle |
Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats Leonardo Perin Ribeiro Inborn aerobic capacity Proteomics 2-DE MS Heart proteins Proteômica Ratos Exercício físico Espectrometria de massas Proteínas |
title_short |
Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats |
title_full |
Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats |
title_fullStr |
Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats |
title_full_unstemmed |
Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats |
title_sort |
Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats |
author |
Leonardo Perin Ribeiro |
author_facet |
Leonardo Perin Ribeiro Leandro Ceotto Freitas Lima Gustavo Baptista Naumann Silvana dos Santos Meyrelles Wellington Lunz Simone da Fonseca Pires Hélida Monteiro de Andrade Juliana Brambilla Carnielli Trindade Suely Gomes de Figueiredo |
author_role |
author |
author2 |
Leandro Ceotto Freitas Lima Gustavo Baptista Naumann Silvana dos Santos Meyrelles Wellington Lunz Simone da Fonseca Pires Hélida Monteiro de Andrade Juliana Brambilla Carnielli Trindade Suely Gomes de Figueiredo |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Leonardo Perin Ribeiro Leandro Ceotto Freitas Lima Gustavo Baptista Naumann Silvana dos Santos Meyrelles Wellington Lunz Simone da Fonseca Pires Hélida Monteiro de Andrade Juliana Brambilla Carnielli Trindade Suely Gomes de Figueiredo |
dc.subject.por.fl_str_mv |
Inborn aerobic capacity Proteomics 2-DE MS Heart proteins |
topic |
Inborn aerobic capacity Proteomics 2-DE MS Heart proteins Proteômica Ratos Exercício físico Espectrometria de massas Proteínas |
dc.subject.other.pt_BR.fl_str_mv |
Proteômica Ratos Exercício físico Espectrometria de massas Proteínas |
description |
In the present study, we successfully demonstrated for the first time the existence of cardiac proteomic differences between non-selectively bred rats with distinct intrinsic exercise capacities. A proteomic approach based on two-dimensional gel electrophoresis coupled to mass spectrometry was used to study the left ventricle (LV) tissue proteome of rats with distinct intrinsic exercise capacity. Low running performance (LRP) and high running performance (HRP) rats were categorized by a treadmill exercise test, according to distance run to exhaustion. The running capacity of HRPs was 3.5-fold greater than LRPs. Protein profiling revealed 29 differences between HRP and LRP rats (15 proteins were identified). We detected alterations in components involved in metabolism, antioxidant and stress response, microfibrillar and cytoskeletal proteins. Contractile proteins were upregulated in the LVs of HRP rats (a-myosin heavy chain-6, myosin light chain-1 and creatine kinase), whereas the LVs of LRP rats exhibited upregulation in proteins associated with stress response (aldehyde dehydrogenase 2, a-crystallin B chain and HSPb-2). In addition, the cytoskeletal proteins desmin and a-actin were upregulated in LRPs. Taken together, our results suggest that the increased contractile protein levels in HRP rats partly accounted for their improved exercise capacity, and that proteins considered risk factors to the development of cardiovascular disease were expressed in higher amounts in LRP animals. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2023-07-14T20:26:34Z |
dc.date.available.fl_str_mv |
2023-07-14T20:26:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/56289 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1590/1414-431X20177033 |
dc.identifier.issn.pt_BR.fl_str_mv |
1414-431X |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0001-9550-6095 https://orcid.org/0000-0003-0167-4093 https://orcid.org/0000-0003-2318-3340 https://orcid.org/0000-0003-2823-4530 https://orcid.org/0000-0003-2116-6379 https://orcid.org/0000-0002-5363-8329 |
url |
https://doi.org/10.1590/1414-431X20177033 http://hdl.handle.net/1843/56289 https://orcid.org/0000-0001-9550-6095 https://orcid.org/0000-0003-0167-4093 https://orcid.org/0000-0003-2318-3340 https://orcid.org/0000-0003-2823-4530 https://orcid.org/0000-0003-2116-6379 https://orcid.org/0000-0002-5363-8329 |
identifier_str_mv |
1414-431X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian Journal of Medical and Biological Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
ICB - DEPARTAMENTO DE PARASITOLOGIA |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
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reponame:Repositório Institucional da UFMG instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
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UFMG |
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UFMG |
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Repositório Institucional da UFMG |
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