V brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | http://hdl.handle.net/1843/54836 |
Resumo: | Background: The V Brazilian Consensus for determination of autoantibodies against cellular constituents on HEp-2 cells, held in Brasilia (DF, Brazil) on August 27, 2016, discussed the harmonization between the Brazilian Consensus on ANA (BCA) guidelines and the International Consensus on ANA Patterns (ICAP) recommendations (www.anapatterns.org). Initial guidelines were formulated by the group of Brazilian experts with the purpose of guiding and enabling Brazilian clinical laboratories to adopt recommendations and to provide a common standard for national and international consensuses. Mainbody: Twenty Brazilian researchers and experts from universities and clinical laboratories representing the various geographical regions of the country participated in the meeting. Three main topics were discussed, namely the harmonization between the BCA guidelines and latest recommendations of the ICAP initiative, the adjustment of the terminology and report on HEp-2 patterns, and a reassessment of quality assurance parameters. For the three topics, our aim was to establish specific guidelines. All recommendations were based on consensus among participants. There was concrete progress in the adjustment of the BCA guidelines to match the ICAP guidelines. To a certain extent, this derives from the fact that ICAP recommendations were largely based on the algorithm and recommendations of the IV Brazilian ANA Consensus, as consistently recognized in the ICAP publications and presentations. However, although there is great overlap between the two Consensuses, there are some point divergences. These specific items were individually and extensively discussed, and it was acknowledged that in several points ICAP improved recommendations previously issued by the Brazilian ANA Consensus and these changes were readily implemented. Regarding some specific topics, the BCA panel of experts felt that the previously issued recommendations remained relevant and possibly will require further discussion with ICAP. The term anti-cell antibodies was adopted as the recommended designation, recognizing that the assay addresses antibodies against antigens in the nucleus and in other cell compartments. However, the acronym ANA HEp-2 was maintained due to historical and regulatory reasons. It was also signalized that the latest trend in ICAP is to adopt the term Indirect Immunofluorescent Assay on HEp-2 cell substrate (HEp-2 IIFA). In addition, the quality assurance strategies previously presented were ratified and emphasized.Conclusion: The V BCA edition was successful in establishing an overall harmonization with the ICAP recommendations for interpretation of the HEp-2 IIFA test, pinpointing the perspectives in filling the remaining gaps between both initiatives. |
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2023-06-12T19:58:38Z2023-06-12T19:58:38Z2019592911110.1186/s42358-019-0069-525233106http://hdl.handle.net/1843/54836Background: The V Brazilian Consensus for determination of autoantibodies against cellular constituents on HEp-2 cells, held in Brasilia (DF, Brazil) on August 27, 2016, discussed the harmonization between the Brazilian Consensus on ANA (BCA) guidelines and the International Consensus on ANA Patterns (ICAP) recommendations (www.anapatterns.org). Initial guidelines were formulated by the group of Brazilian experts with the purpose of guiding and enabling Brazilian clinical laboratories to adopt recommendations and to provide a common standard for national and international consensuses. Mainbody: Twenty Brazilian researchers and experts from universities and clinical laboratories representing the various geographical regions of the country participated in the meeting. Three main topics were discussed, namely the harmonization between the BCA guidelines and latest recommendations of the ICAP initiative, the adjustment of the terminology and report on HEp-2 patterns, and a reassessment of quality assurance parameters. For the three topics, our aim was to establish specific guidelines. All recommendations were based on consensus among participants. There was concrete progress in the adjustment of the BCA guidelines to match the ICAP guidelines. To a certain extent, this derives from the fact that ICAP recommendations were largely based on the algorithm and recommendations of the IV Brazilian ANA Consensus, as consistently recognized in the ICAP publications and presentations. However, although there is great overlap between the two Consensuses, there are some point divergences. These specific items were individually and extensively discussed, and it was acknowledged that in several points ICAP improved recommendations previously issued by the Brazilian ANA Consensus and these changes were readily implemented. Regarding some specific topics, the BCA panel of experts felt that the previously issued recommendations remained relevant and possibly will require further discussion with ICAP. The term anti-cell antibodies was adopted as the recommended designation, recognizing that the assay addresses antibodies against antigens in the nucleus and in other cell compartments. However, the acronym ANA HEp-2 was maintained due to historical and regulatory reasons. It was also signalized that the latest trend in ICAP is to adopt the term Indirect Immunofluorescent Assay on HEp-2 cell substrate (HEp-2 IIFA). In addition, the quality assurance strategies previously presented were ratified and emphasized.Conclusion: The V BCA edition was successful in establishing an overall harmonization with the ICAP recommendations for interpretation of the HEp-2 IIFA test, pinpointing the perspectives in filling the remaining gaps between both initiatives.engUniversidade Federal de Minas GeraisUFMGBrasilMED - DEPARTAMENTO DE PROPEDÊUTICA COMPLEMENTARAdvances in RheumatologyAutoanticorposCélulas Hep G2Anticorpos AntinuclearesTécnica Indireta de Fluorescência para AnticorpoPadrões de ReferênciaANAAutoantibodiesHEp-2 cellsAntinuclear antibodiesIndirect immunofluorescenceStandardizationV brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://advancesinrheumatology.biomedcentral.com/articles/10.1186/s42358-019-0069-5Wilson de MelocruvinelFabiano de Almeida BritoFernanda Bull FlumianGlaucielen Gomes da SilvaJozelia RêgoLisiane Maria Ericoni Dos AnjosNatasha SlhessarenkoSandra Gofinet PasotoSuzane Pretti Figueiredo NevesValéria ValimWilton Silva Dos SantosLuis Eduardo Coelho AndradePaulo Luiz Carvalho FrancescantonioCarlos Alberto Von MühlenAlessandra DellavanceAntônio Carlos XimenesCarlos David BicharaCleonice BuenoCristóvão Luis Pitangueira MangueiraEloísa Bonfáapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/54836/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALV Brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells pdfa.pdfV Brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells pdfa.pdfapplication/pdf24974086https://repositorio.ufmg.br/bitstream/1843/54836/2/V%20Brazilian%20consensus%20guidelines%20for%20detection%20of%20anti-cell%20autoantibodies%20on%20hep-2%20cells%20pdfa.pdfbec3c1bcfb4926991324cd5b2d0b02e6MD521843/548362023-06-12 18:26:21.866oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-06-12T21:26:21Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
V brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells |
title |
V brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells |
spellingShingle |
V brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells Wilson de Melocruvinel ANA Autoantibodies HEp-2 cells Antinuclear antibodies Indirect immunofluorescence Standardization Autoanticorpos Células Hep G2 Anticorpos Antinucleares Técnica Indireta de Fluorescência para Anticorpo Padrões de Referência |
title_short |
V brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells |
title_full |
V brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells |
title_fullStr |
V brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells |
title_full_unstemmed |
V brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells |
title_sort |
V brazilian consensus guidelines for detection of anti-cell autoantibodies on hep-2 cells |
author |
Wilson de Melocruvinel |
author_facet |
Wilson de Melocruvinel Fabiano de Almeida Brito Fernanda Bull Flumian Glaucielen Gomes da Silva Jozelia Rêgo Lisiane Maria Ericoni Dos Anjos Natasha Slhessarenko Sandra Gofinet Pasoto Suzane Pretti Figueiredo Neves Valéria Valim Wilton Silva Dos Santos Luis Eduardo Coelho Andrade Paulo Luiz Carvalho Francescantonio Carlos Alberto Von Mühlen Alessandra Dellavance Antônio Carlos Ximenes Carlos David Bichara Cleonice Bueno Cristóvão Luis Pitangueira Mangueira Eloísa Bonfá |
author_role |
author |
author2 |
Fabiano de Almeida Brito Fernanda Bull Flumian Glaucielen Gomes da Silva Jozelia Rêgo Lisiane Maria Ericoni Dos Anjos Natasha Slhessarenko Sandra Gofinet Pasoto Suzane Pretti Figueiredo Neves Valéria Valim Wilton Silva Dos Santos Luis Eduardo Coelho Andrade Paulo Luiz Carvalho Francescantonio Carlos Alberto Von Mühlen Alessandra Dellavance Antônio Carlos Ximenes Carlos David Bichara Cleonice Bueno Cristóvão Luis Pitangueira Mangueira Eloísa Bonfá |
author2_role |
author author author author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Wilson de Melocruvinel Fabiano de Almeida Brito Fernanda Bull Flumian Glaucielen Gomes da Silva Jozelia Rêgo Lisiane Maria Ericoni Dos Anjos Natasha Slhessarenko Sandra Gofinet Pasoto Suzane Pretti Figueiredo Neves Valéria Valim Wilton Silva Dos Santos Luis Eduardo Coelho Andrade Paulo Luiz Carvalho Francescantonio Carlos Alberto Von Mühlen Alessandra Dellavance Antônio Carlos Ximenes Carlos David Bichara Cleonice Bueno Cristóvão Luis Pitangueira Mangueira Eloísa Bonfá |
dc.subject.por.fl_str_mv |
ANA Autoantibodies HEp-2 cells Antinuclear antibodies Indirect immunofluorescence Standardization |
topic |
ANA Autoantibodies HEp-2 cells Antinuclear antibodies Indirect immunofluorescence Standardization Autoanticorpos Células Hep G2 Anticorpos Antinucleares Técnica Indireta de Fluorescência para Anticorpo Padrões de Referência |
dc.subject.other.pt_BR.fl_str_mv |
Autoanticorpos Células Hep G2 Anticorpos Antinucleares Técnica Indireta de Fluorescência para Anticorpo Padrões de Referência |
description |
Background: The V Brazilian Consensus for determination of autoantibodies against cellular constituents on HEp-2 cells, held in Brasilia (DF, Brazil) on August 27, 2016, discussed the harmonization between the Brazilian Consensus on ANA (BCA) guidelines and the International Consensus on ANA Patterns (ICAP) recommendations (www.anapatterns.org). Initial guidelines were formulated by the group of Brazilian experts with the purpose of guiding and enabling Brazilian clinical laboratories to adopt recommendations and to provide a common standard for national and international consensuses. Mainbody: Twenty Brazilian researchers and experts from universities and clinical laboratories representing the various geographical regions of the country participated in the meeting. Three main topics were discussed, namely the harmonization between the BCA guidelines and latest recommendations of the ICAP initiative, the adjustment of the terminology and report on HEp-2 patterns, and a reassessment of quality assurance parameters. For the three topics, our aim was to establish specific guidelines. All recommendations were based on consensus among participants. There was concrete progress in the adjustment of the BCA guidelines to match the ICAP guidelines. To a certain extent, this derives from the fact that ICAP recommendations were largely based on the algorithm and recommendations of the IV Brazilian ANA Consensus, as consistently recognized in the ICAP publications and presentations. However, although there is great overlap between the two Consensuses, there are some point divergences. These specific items were individually and extensively discussed, and it was acknowledged that in several points ICAP improved recommendations previously issued by the Brazilian ANA Consensus and these changes were readily implemented. Regarding some specific topics, the BCA panel of experts felt that the previously issued recommendations remained relevant and possibly will require further discussion with ICAP. The term anti-cell antibodies was adopted as the recommended designation, recognizing that the assay addresses antibodies against antigens in the nucleus and in other cell compartments. However, the acronym ANA HEp-2 was maintained due to historical and regulatory reasons. It was also signalized that the latest trend in ICAP is to adopt the term Indirect Immunofluorescent Assay on HEp-2 cell substrate (HEp-2 IIFA). In addition, the quality assurance strategies previously presented were ratified and emphasized.Conclusion: The V BCA edition was successful in establishing an overall harmonization with the ICAP recommendations for interpretation of the HEp-2 IIFA test, pinpointing the perspectives in filling the remaining gaps between both initiatives. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019 |
dc.date.accessioned.fl_str_mv |
2023-06-12T19:58:38Z |
dc.date.available.fl_str_mv |
2023-06-12T19:58:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/54836 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1186/s42358-019-0069-5 |
dc.identifier.issn.pt_BR.fl_str_mv |
25233106 |
identifier_str_mv |
10.1186/s42358-019-0069-5 25233106 |
url |
http://hdl.handle.net/1843/54836 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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Advances in Rheumatology |
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openAccess |
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Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
MED - DEPARTAMENTO DE PROPEDÊUTICA COMPLEMENTAR |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
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UFMG |
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UFMG |
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Repositório Institucional da UFMG |
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