Site-specific microinjection of liposomes into the brain for local infusion of a short-lived peptide.
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/3626 https://doi.org/10.1016/j.jconrel.2003.12.010 |
Resumo: | The short-lived peptide, angiotensin-(1-7) (Ang-(1-7)), was encapsulated in different liposome preparations, in order to evaluate the influence of membrane fluidity, membrane surface, liposome size and dose of peptide on the cardiovascular effects of the encapsulated peptide at a specific site of the brain. These preparations were microinjected unilaterally into the rostral ventrolateral medulla (RVLM) of Wistar rats, and mean arterial blood pressure (MAP) and heart rate (HR) were registered by telemetry. Pegylated, rigid and calibrated (200 nm) liposomes, containing 50 ng of Ang-(1-7), elicited a significant increase of MAP for at least 7 days, in contrast to empty liposomes or non-pegylated liposomes. When a two-fold higher peptide dose was employed or when pegylated liposomes were used in the fluid state or uncalibrated, less pronounced pressor effects were observed. These data show that the cardiovascular responses to the microinjection of Ang-(1-7)- containing liposomes into the RVLM can be modulated through the manipulation of liposome characteristics. These results can be explained by the influence of liposome characteristics on the flux of peptide release. It is expected that this new method will encounter numerous applications in the study of the chronic actions of short-lived bioactive peptides in specific sites of the brain. |
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Barcellos, Neila Marcia SilvaCaligiorne, Sordaini MariaSantos, Robson Augusto Souza dosFrezard, Frederic Jean Georges2014-09-09T18:15:14Z2014-09-09T18:15:14Z2004BARCELLOS, N. M. S. et al. Site-specific microinjection of liposomes into the brain for local infusion of a short-lived peptide. Journal Controlled Release, v. 95, p. 301-307, 2004. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0168365903005777>. Acesso em: 20 ago. 2014.0168-3659http://www.repositorio.ufop.br/handle/123456789/3626https://doi.org/10.1016/j.jconrel.2003.12.010The short-lived peptide, angiotensin-(1-7) (Ang-(1-7)), was encapsulated in different liposome preparations, in order to evaluate the influence of membrane fluidity, membrane surface, liposome size and dose of peptide on the cardiovascular effects of the encapsulated peptide at a specific site of the brain. These preparations were microinjected unilaterally into the rostral ventrolateral medulla (RVLM) of Wistar rats, and mean arterial blood pressure (MAP) and heart rate (HR) were registered by telemetry. Pegylated, rigid and calibrated (200 nm) liposomes, containing 50 ng of Ang-(1-7), elicited a significant increase of MAP for at least 7 days, in contrast to empty liposomes or non-pegylated liposomes. When a two-fold higher peptide dose was employed or when pegylated liposomes were used in the fluid state or uncalibrated, less pronounced pressor effects were observed. These data show that the cardiovascular responses to the microinjection of Ang-(1-7)- containing liposomes into the RVLM can be modulated through the manipulation of liposome characteristics. These results can be explained by the influence of liposome characteristics on the flux of peptide release. It is expected that this new method will encounter numerous applications in the study of the chronic actions of short-lived bioactive peptides in specific sites of the brain.LiposomesAngiotensin-(1–7)PegylationRostral ventrolateral medullaRenin-angiotensin systemSite-specific microinjection of liposomes into the brain for local infusion of a short-lived peptide.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleO periódico Journal Controlled Release concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3456621097721.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://www.repositorio.ufop.br/bitstream/123456789/3626/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52ORIGINALARTIGO_SiteSpecificMicroinjetion.pdfARTIGO_SiteSpecificMicroinjetion.pdfapplication/pdf282165http://www.repositorio.ufop.br/bitstream/123456789/3626/1/ARTIGO_SiteSpecificMicroinjetion.pdfb1b4d0f6ded6d8fe2f02203075996806MD51123456789/36262019-04-30 09:51:48.056oai:localhost: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Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-04-30T13:51:48Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Site-specific microinjection of liposomes into the brain for local infusion of a short-lived peptide. |
title |
Site-specific microinjection of liposomes into the brain for local infusion of a short-lived peptide. |
spellingShingle |
Site-specific microinjection of liposomes into the brain for local infusion of a short-lived peptide. Barcellos, Neila Marcia Silva Liposomes Angiotensin-(1–7) Pegylation Rostral ventrolateral medulla Renin-angiotensin system |
title_short |
Site-specific microinjection of liposomes into the brain for local infusion of a short-lived peptide. |
title_full |
Site-specific microinjection of liposomes into the brain for local infusion of a short-lived peptide. |
title_fullStr |
Site-specific microinjection of liposomes into the brain for local infusion of a short-lived peptide. |
title_full_unstemmed |
Site-specific microinjection of liposomes into the brain for local infusion of a short-lived peptide. |
title_sort |
Site-specific microinjection of liposomes into the brain for local infusion of a short-lived peptide. |
author |
Barcellos, Neila Marcia Silva |
author_facet |
Barcellos, Neila Marcia Silva Caligiorne, Sordaini Maria Santos, Robson Augusto Souza dos Frezard, Frederic Jean Georges |
author_role |
author |
author2 |
Caligiorne, Sordaini Maria Santos, Robson Augusto Souza dos Frezard, Frederic Jean Georges |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Barcellos, Neila Marcia Silva Caligiorne, Sordaini Maria Santos, Robson Augusto Souza dos Frezard, Frederic Jean Georges |
dc.subject.por.fl_str_mv |
Liposomes Angiotensin-(1–7) Pegylation Rostral ventrolateral medulla Renin-angiotensin system |
topic |
Liposomes Angiotensin-(1–7) Pegylation Rostral ventrolateral medulla Renin-angiotensin system |
description |
The short-lived peptide, angiotensin-(1-7) (Ang-(1-7)), was encapsulated in different liposome preparations, in order to evaluate the influence of membrane fluidity, membrane surface, liposome size and dose of peptide on the cardiovascular effects of the encapsulated peptide at a specific site of the brain. These preparations were microinjected unilaterally into the rostral ventrolateral medulla (RVLM) of Wistar rats, and mean arterial blood pressure (MAP) and heart rate (HR) were registered by telemetry. Pegylated, rigid and calibrated (200 nm) liposomes, containing 50 ng of Ang-(1-7), elicited a significant increase of MAP for at least 7 days, in contrast to empty liposomes or non-pegylated liposomes. When a two-fold higher peptide dose was employed or when pegylated liposomes were used in the fluid state or uncalibrated, less pronounced pressor effects were observed. These data show that the cardiovascular responses to the microinjection of Ang-(1-7)- containing liposomes into the RVLM can be modulated through the manipulation of liposome characteristics. These results can be explained by the influence of liposome characteristics on the flux of peptide release. It is expected that this new method will encounter numerous applications in the study of the chronic actions of short-lived bioactive peptides in specific sites of the brain. |
publishDate |
2004 |
dc.date.issued.fl_str_mv |
2004 |
dc.date.accessioned.fl_str_mv |
2014-09-09T18:15:14Z |
dc.date.available.fl_str_mv |
2014-09-09T18:15:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BARCELLOS, N. M. S. et al. Site-specific microinjection of liposomes into the brain for local infusion of a short-lived peptide. Journal Controlled Release, v. 95, p. 301-307, 2004. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0168365903005777>. Acesso em: 20 ago. 2014. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/3626 |
dc.identifier.issn.none.fl_str_mv |
0168-3659 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.jconrel.2003.12.010 |
identifier_str_mv |
BARCELLOS, N. M. S. et al. Site-specific microinjection of liposomes into the brain for local infusion of a short-lived peptide. Journal Controlled Release, v. 95, p. 301-307, 2004. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0168365903005777>. Acesso em: 20 ago. 2014. 0168-3659 |
url |
http://www.repositorio.ufop.br/handle/123456789/3626 https://doi.org/10.1016/j.jconrel.2003.12.010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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