Molecular characterization of nontuberculous Mycobacteria in a tuberculosis and HIV reference unit in the State of Amazonas, Brazil.

• Autor(a) principal: Lima, Ana Carolina de Oliveira de
• Data de Publicação: 2022
• Outros Autores: Schmid, Karen Barros, Melo, Hilda Ferreira de, Athayde, Rafaella Christine, Monte, Rossiclea Lins, Almeida, Isabela Neves de, Miranda, Silvana Spíndola de, Kritski, Afrânio, Rossetti, Maria Lucia Rosa, Santos, Marcelo Cordeiro dos
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFOP
• Texto Completo: http://www.repositorio.ufop.br/jspui/handle/123456789/17573; https://doi.org/10.1590/0037-8682-0613-2021
• topic: HIV infections; Molecular diagnostic technique; Nontuberculous mycobacteria; Prevalence

Background: In recent years, the prevalence of nontuberculous mycobacterial (NTM) infections has increased in different regions of the world. The American Thoracic Society (ATS) recommends standardized identification criteria, reinforcing the need for faster and less complicated clinical and laboratory techniques. Methods: In this retrospective study, NTM species isolated from pulmonary, extrapulmonary, and disseminated samples from patients treated at a TB/HIV reference unit in the State of Amazonas from 2011 to 2014 were identified through a combination of molecular techniques. Results: To identify the molecular technique, 50 cryopreserved NTM cultures were recovered and subcultivated in culture medium. The potentially pathogenic NTM species identified were M. avium, M. intracellulare, M. kansasii, M. chelonae, M. abscessus, M. fortuitum, and M. peregrinum. Results of GenoType® showed moderate agreement with those of genomic sequencing (kappa = 0.60), whereas the results obtained by the PRA-hsp65 technique disagreed with the results obtained by sequencing (kappa = 0.49). Conclusions: Our findings highlight that GenoType CM is a good method for the identification of NTM, as well as the need for the application of standardized criteria, such as those set forth by the ATS.