Antibody responses induced by Leish-Tec®, an A2-basedvaccine for visceral leishmaniasis, in a heterogeneous caninepopulation.
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/3769 https://doi.org/10.1016/j.vetpar.2014.04.025 |
Resumo: | Zoonotic visceral leishmaniasis (VL) is a widespread disease, and dogs are the main reser-voirs for human parasite transmission. Hence, development of an effective vaccine thatprevents disease and reduces the transmission of VL is required. As euthanasia of seropos-itive dogs is recommended in Brazil for VL epidemiological control, to include anti-VLcanine vaccines as a mass control measure it is necessary to characterize the humoralresponses induced by vaccination and if they interfere with the reactivity of vaccinateddogs in serological diagnostic tests. Leish-Tec®is an amastigote-specific A2 recombinantprotein vaccine against canine visceral leishmaniasis (CVL) that is commercially availablein Brazil. Here, we tested the immunogenicity of Leish-Tec®in a heterogeneous dog popula-tion by measuring A2-specific antibody responses. Healthy dogs (n = 140) of various breedswere allocated to two groups: one group received Leish-Tec®(n = 70), and the other groupreceived a placebo (n = 70). Anti-A2 or anti-Leishmania promastigote antigen (LPA) antibodylevels were measured by ELISA in serum samples collected before and after vaccination.An immunochromatographic test (DPP) based on the recombinant K28 antigen was alsoused for serodiagnosis of CVL. Vaccinated animals, except one, remained seronegative foranti-LPA total IgG and anti-K28 antibodies. Conversely, seropositivity for anti-A2 total IgGantibodies was found in 98% of animals after vaccination. This value decreased to 81.13% at6 months before rising again (98%), after the vaccination boost. Anti-A2 IgG2 and IgG1 titers. |
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Testasicca, Miriam Conceição de SouzaSantos, Mariana Silva dosMachado, Leopoldo MarquesSerufo, Ângela VieiraDoro, DanielAvelar, DanielTibúrcio, Ana Maria LeonardiAbrantes, Christiane de FreitasCoelho, George Luiz Lins MachadoGrimaldi Junior, GabrielGazzinelli, Ricardo TostesFernandes, Ana Paula2014-11-11T18:10:25Z2014-11-11T18:10:25Z2014TESTASICCA, M. C. S. et al. Antibody responses induced by Leish-Tec®, an A2-basedvaccine for visceral leishmaniasis, in a heterogeneous caninepopulation. Veterinary Parasitology, v. 14, p. 234-239, 2014. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0304401714002349>. Acesso em: 01 set. 2014.0304-4017http://www.repositorio.ufop.br/handle/123456789/3769https://doi.org/10.1016/j.vetpar.2014.04.025Zoonotic visceral leishmaniasis (VL) is a widespread disease, and dogs are the main reser-voirs for human parasite transmission. Hence, development of an effective vaccine thatprevents disease and reduces the transmission of VL is required. As euthanasia of seropos-itive dogs is recommended in Brazil for VL epidemiological control, to include anti-VLcanine vaccines as a mass control measure it is necessary to characterize the humoralresponses induced by vaccination and if they interfere with the reactivity of vaccinateddogs in serological diagnostic tests. Leish-Tec®is an amastigote-specific A2 recombinantprotein vaccine against canine visceral leishmaniasis (CVL) that is commercially availablein Brazil. Here, we tested the immunogenicity of Leish-Tec®in a heterogeneous dog popula-tion by measuring A2-specific antibody responses. Healthy dogs (n = 140) of various breedswere allocated to two groups: one group received Leish-Tec®(n = 70), and the other groupreceived a placebo (n = 70). Anti-A2 or anti-Leishmania promastigote antigen (LPA) antibodylevels were measured by ELISA in serum samples collected before and after vaccination.An immunochromatographic test (DPP) based on the recombinant K28 antigen was alsoused for serodiagnosis of CVL. Vaccinated animals, except one, remained seronegative foranti-LPA total IgG and anti-K28 antibodies. Conversely, seropositivity for anti-A2 total IgGantibodies was found in 98% of animals after vaccination. This value decreased to 81.13% at6 months before rising again (98%), after the vaccination boost. Anti-A2 IgG2 and IgG1 titers.Visceral leishmaniasisVaccineLeish-TecDogsAntibodiesAntibody responses induced by Leish-Tec®, an A2-basedvaccine for visceral leishmaniasis, in a heterogeneous caninepopulation.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleO periódico Veterinary Parasitology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3461450204375.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://www.repositorio.ufop.br/bitstream/123456789/3769/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52ORIGINALARTIGO_AntibodyresponseInduced.pdfARTIGO_AntibodyresponseInduced.pdfapplication/pdf370626http://www.repositorio.ufop.br/bitstream/123456789/3769/1/ARTIGO_AntibodyresponseInduced.pdfbb08f717f37b11207bb51e63480e3fd5MD51123456789/37692019-05-07 10:39:15.549oai:localhost: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Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-05-07T14:39:15Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Antibody responses induced by Leish-Tec®, an A2-basedvaccine for visceral leishmaniasis, in a heterogeneous caninepopulation. |
title |
Antibody responses induced by Leish-Tec®, an A2-basedvaccine for visceral leishmaniasis, in a heterogeneous caninepopulation. |
spellingShingle |
Antibody responses induced by Leish-Tec®, an A2-basedvaccine for visceral leishmaniasis, in a heterogeneous caninepopulation. Testasicca, Miriam Conceição de Souza Visceral leishmaniasis Vaccine Leish-Tec Dogs Antibodies |
title_short |
Antibody responses induced by Leish-Tec®, an A2-basedvaccine for visceral leishmaniasis, in a heterogeneous caninepopulation. |
title_full |
Antibody responses induced by Leish-Tec®, an A2-basedvaccine for visceral leishmaniasis, in a heterogeneous caninepopulation. |
title_fullStr |
Antibody responses induced by Leish-Tec®, an A2-basedvaccine for visceral leishmaniasis, in a heterogeneous caninepopulation. |
title_full_unstemmed |
Antibody responses induced by Leish-Tec®, an A2-basedvaccine for visceral leishmaniasis, in a heterogeneous caninepopulation. |
title_sort |
Antibody responses induced by Leish-Tec®, an A2-basedvaccine for visceral leishmaniasis, in a heterogeneous caninepopulation. |
author |
Testasicca, Miriam Conceição de Souza |
author_facet |
Testasicca, Miriam Conceição de Souza Santos, Mariana Silva dos Machado, Leopoldo Marques Serufo, Ângela Vieira Doro, Daniel Avelar, Daniel Tibúrcio, Ana Maria Leonardi Abrantes, Christiane de Freitas Coelho, George Luiz Lins Machado Grimaldi Junior, Gabriel Gazzinelli, Ricardo Tostes Fernandes, Ana Paula |
author_role |
author |
author2 |
Santos, Mariana Silva dos Machado, Leopoldo Marques Serufo, Ângela Vieira Doro, Daniel Avelar, Daniel Tibúrcio, Ana Maria Leonardi Abrantes, Christiane de Freitas Coelho, George Luiz Lins Machado Grimaldi Junior, Gabriel Gazzinelli, Ricardo Tostes Fernandes, Ana Paula |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Testasicca, Miriam Conceição de Souza Santos, Mariana Silva dos Machado, Leopoldo Marques Serufo, Ângela Vieira Doro, Daniel Avelar, Daniel Tibúrcio, Ana Maria Leonardi Abrantes, Christiane de Freitas Coelho, George Luiz Lins Machado Grimaldi Junior, Gabriel Gazzinelli, Ricardo Tostes Fernandes, Ana Paula |
dc.subject.por.fl_str_mv |
Visceral leishmaniasis Vaccine Leish-Tec Dogs Antibodies |
topic |
Visceral leishmaniasis Vaccine Leish-Tec Dogs Antibodies |
description |
Zoonotic visceral leishmaniasis (VL) is a widespread disease, and dogs are the main reser-voirs for human parasite transmission. Hence, development of an effective vaccine thatprevents disease and reduces the transmission of VL is required. As euthanasia of seropos-itive dogs is recommended in Brazil for VL epidemiological control, to include anti-VLcanine vaccines as a mass control measure it is necessary to characterize the humoralresponses induced by vaccination and if they interfere with the reactivity of vaccinateddogs in serological diagnostic tests. Leish-Tec®is an amastigote-specific A2 recombinantprotein vaccine against canine visceral leishmaniasis (CVL) that is commercially availablein Brazil. Here, we tested the immunogenicity of Leish-Tec®in a heterogeneous dog popula-tion by measuring A2-specific antibody responses. Healthy dogs (n = 140) of various breedswere allocated to two groups: one group received Leish-Tec®(n = 70), and the other groupreceived a placebo (n = 70). Anti-A2 or anti-Leishmania promastigote antigen (LPA) antibodylevels were measured by ELISA in serum samples collected before and after vaccination.An immunochromatographic test (DPP) based on the recombinant K28 antigen was alsoused for serodiagnosis of CVL. Vaccinated animals, except one, remained seronegative foranti-LPA total IgG and anti-K28 antibodies. Conversely, seropositivity for anti-A2 total IgGantibodies was found in 98% of animals after vaccination. This value decreased to 81.13% at6 months before rising again (98%), after the vaccination boost. Anti-A2 IgG2 and IgG1 titers. |
publishDate |
2014 |
dc.date.accessioned.fl_str_mv |
2014-11-11T18:10:25Z |
dc.date.available.fl_str_mv |
2014-11-11T18:10:25Z |
dc.date.issued.fl_str_mv |
2014 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
TESTASICCA, M. C. S. et al. Antibody responses induced by Leish-Tec®, an A2-basedvaccine for visceral leishmaniasis, in a heterogeneous caninepopulation. Veterinary Parasitology, v. 14, p. 234-239, 2014. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0304401714002349>. Acesso em: 01 set. 2014. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/3769 |
dc.identifier.issn.none.fl_str_mv |
0304-4017 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.vetpar.2014.04.025 |
identifier_str_mv |
TESTASICCA, M. C. S. et al. Antibody responses induced by Leish-Tec®, an A2-basedvaccine for visceral leishmaniasis, in a heterogeneous caninepopulation. Veterinary Parasitology, v. 14, p. 234-239, 2014. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0304401714002349>. Acesso em: 01 set. 2014. 0304-4017 |
url |
http://www.repositorio.ufop.br/handle/123456789/3769 https://doi.org/10.1016/j.vetpar.2014.04.025 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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