Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis.
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/6629 https://doi.org/10.7717/peerj.1734/supp-1 |
Resumo: | Citrus canker is a major disease affecting citrus production in Brazil. It's mainly caused by Xanthomonas citri subsp. citri strain 306 pathotype A (Xac). We analysed the differential expression of proteins secreted by wild type Xac and an asymptomatic mutant for hrpB4 (1hrpB4) grown in Nutrient Broth (NB) and a medium mimicking growth conditions in the plant (XAM1). This allowed the identification of 55 secreted proteins, of which 37 were secreted by both strains when cultured in XAM1. In this secreted protein repertoire, the following stand out: Virk, Polyphosphate-selective porin, Cellulase, Endoglucanase, Histone-like protein, Ribosomal proteins, five hypothetical proteins expressed only in the wild type strain, Lytic murein transglycosylase, Lipoprotein, Leucyl-tRNA synthetase, Co-chaperonin, Toluene tolerance, C-type cytochrome biogenesis membrane protein, Aminopeptidase and two hypothetical proteins expressed only in the1hrpB4 mutant. Furthermore, Peptidoglycan-associated outer membrane protein, Regulator of pathogenicity factor, Outer membrane proteins, Endopolygalacturonase, Chorismate mutase, Peptidyl-prolyl cis-trans isomerase and seven hypothetical proteins were detected in both strains, suggesting that there was no relationship with the secretion mediated by the type III secretory system, which is not functional in the mutant strain. Also worth mentioning is the Elongation factor Tu (EF-Tu), expressed only the wild type strain, and Type IV pilus assembly protein, Flagellin (FliC) and Flagellar hook-associated protein, identified in the wild-type strain secretome when grown only in NB. Noteworthy, that FliC, EF-Tu are classically characterized as PAMPs (Pathogen-associated molecular patterns), responsible for a PAMP-triggered immunity response. Therefore, our results highlight proteins potentially involved with the virulence. Overall, we conclude that the use of secretome data is a valuable approach that may bring more knowledge of the biology of this important plant pathogen, which ultimately can lead to the establishment of new strategies to combat citrus canker. |
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Ferreira, Rafael MariniMoreira, Leandro MarcioFerro, Jesus AparecidoSilva, Marcia Regina Soares daLaia, Marcelo Luiz deVarani, Alessandro de MelloOliveira, Julio Cezar Franco deFerro, Maria Inês Tiraboschi2016-07-25T16:21:33Z2016-07-25T16:21:33Z2016FERREIRA, F. M. et al. Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis. PEERJ, v. 4, p. e1734, 2016. Disponível em: <https://peerj.com/articles/1734/#additional-information>. Acesso em: 16 jun. 2016.2167-9843 http://www.repositorio.ufop.br/handle/123456789/6629https://doi.org/10.7717/peerj.1734/supp-1Citrus canker is a major disease affecting citrus production in Brazil. It's mainly caused by Xanthomonas citri subsp. citri strain 306 pathotype A (Xac). We analysed the differential expression of proteins secreted by wild type Xac and an asymptomatic mutant for hrpB4 (1hrpB4) grown in Nutrient Broth (NB) and a medium mimicking growth conditions in the plant (XAM1). This allowed the identification of 55 secreted proteins, of which 37 were secreted by both strains when cultured in XAM1. In this secreted protein repertoire, the following stand out: Virk, Polyphosphate-selective porin, Cellulase, Endoglucanase, Histone-like protein, Ribosomal proteins, five hypothetical proteins expressed only in the wild type strain, Lytic murein transglycosylase, Lipoprotein, Leucyl-tRNA synthetase, Co-chaperonin, Toluene tolerance, C-type cytochrome biogenesis membrane protein, Aminopeptidase and two hypothetical proteins expressed only in the1hrpB4 mutant. Furthermore, Peptidoglycan-associated outer membrane protein, Regulator of pathogenicity factor, Outer membrane proteins, Endopolygalacturonase, Chorismate mutase, Peptidyl-prolyl cis-trans isomerase and seven hypothetical proteins were detected in both strains, suggesting that there was no relationship with the secretion mediated by the type III secretory system, which is not functional in the mutant strain. Also worth mentioning is the Elongation factor Tu (EF-Tu), expressed only the wild type strain, and Type IV pilus assembly protein, Flagellin (FliC) and Flagellar hook-associated protein, identified in the wild-type strain secretome when grown only in NB. Noteworthy, that FliC, EF-Tu are classically characterized as PAMPs (Pathogen-associated molecular patterns), responsible for a PAMP-triggered immunity response. Therefore, our results highlight proteins potentially involved with the virulence. Overall, we conclude that the use of secretome data is a valuable approach that may bring more knowledge of the biology of this important plant pathogen, which ultimately can lead to the establishment of new strategies to combat citrus canker.Distributed under Creative Commons CC-BY 4.0 which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fonte: o próprio artigo.info:eu-repo/semantics/openAccessPlant pathogen interactionMedium inducing pathogenicityType III secretion systemAsymptomatic mutantVirulenceUnravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-8924http://www.repositorio.ufop.br/bitstream/123456789/6629/2/license.txt62604f8d955274beb56c80ce1ee5dcaeMD52ORIGINALARTIGO_UnravellingPotentialVirulence.pdfARTIGO_UnravellingPotentialVirulence.pdfapplication/pdf2472498http://www.repositorio.ufop.br/bitstream/123456789/6629/1/ARTIGO_UnravellingPotentialVirulence.pdfe877f136eb8c61ad8f399acaed3ac97bMD51123456789/66292019-09-20 10:54:38.05oai:localhost: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ório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-09-20T14:54:38Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis. |
title |
Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis. |
spellingShingle |
Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis. Ferreira, Rafael Marini Plant pathogen interaction Medium inducing pathogenicity Type III secretion system Asymptomatic mutant Virulence |
title_short |
Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis. |
title_full |
Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis. |
title_fullStr |
Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis. |
title_full_unstemmed |
Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis. |
title_sort |
Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis. |
author |
Ferreira, Rafael Marini |
author_facet |
Ferreira, Rafael Marini Moreira, Leandro Marcio Ferro, Jesus Aparecido Silva, Marcia Regina Soares da Laia, Marcelo Luiz de Varani, Alessandro de Mello Oliveira, Julio Cezar Franco de Ferro, Maria Inês Tiraboschi |
author_role |
author |
author2 |
Moreira, Leandro Marcio Ferro, Jesus Aparecido Silva, Marcia Regina Soares da Laia, Marcelo Luiz de Varani, Alessandro de Mello Oliveira, Julio Cezar Franco de Ferro, Maria Inês Tiraboschi |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira, Rafael Marini Moreira, Leandro Marcio Ferro, Jesus Aparecido Silva, Marcia Regina Soares da Laia, Marcelo Luiz de Varani, Alessandro de Mello Oliveira, Julio Cezar Franco de Ferro, Maria Inês Tiraboschi |
dc.subject.por.fl_str_mv |
Plant pathogen interaction Medium inducing pathogenicity Type III secretion system Asymptomatic mutant Virulence |
topic |
Plant pathogen interaction Medium inducing pathogenicity Type III secretion system Asymptomatic mutant Virulence |
description |
Citrus canker is a major disease affecting citrus production in Brazil. It's mainly caused by Xanthomonas citri subsp. citri strain 306 pathotype A (Xac). We analysed the differential expression of proteins secreted by wild type Xac and an asymptomatic mutant for hrpB4 (1hrpB4) grown in Nutrient Broth (NB) and a medium mimicking growth conditions in the plant (XAM1). This allowed the identification of 55 secreted proteins, of which 37 were secreted by both strains when cultured in XAM1. In this secreted protein repertoire, the following stand out: Virk, Polyphosphate-selective porin, Cellulase, Endoglucanase, Histone-like protein, Ribosomal proteins, five hypothetical proteins expressed only in the wild type strain, Lytic murein transglycosylase, Lipoprotein, Leucyl-tRNA synthetase, Co-chaperonin, Toluene tolerance, C-type cytochrome biogenesis membrane protein, Aminopeptidase and two hypothetical proteins expressed only in the1hrpB4 mutant. Furthermore, Peptidoglycan-associated outer membrane protein, Regulator of pathogenicity factor, Outer membrane proteins, Endopolygalacturonase, Chorismate mutase, Peptidyl-prolyl cis-trans isomerase and seven hypothetical proteins were detected in both strains, suggesting that there was no relationship with the secretion mediated by the type III secretory system, which is not functional in the mutant strain. Also worth mentioning is the Elongation factor Tu (EF-Tu), expressed only the wild type strain, and Type IV pilus assembly protein, Flagellin (FliC) and Flagellar hook-associated protein, identified in the wild-type strain secretome when grown only in NB. Noteworthy, that FliC, EF-Tu are classically characterized as PAMPs (Pathogen-associated molecular patterns), responsible for a PAMP-triggered immunity response. Therefore, our results highlight proteins potentially involved with the virulence. Overall, we conclude that the use of secretome data is a valuable approach that may bring more knowledge of the biology of this important plant pathogen, which ultimately can lead to the establishment of new strategies to combat citrus canker. |
publishDate |
2016 |
dc.date.accessioned.fl_str_mv |
2016-07-25T16:21:33Z |
dc.date.available.fl_str_mv |
2016-07-25T16:21:33Z |
dc.date.issued.fl_str_mv |
2016 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FERREIRA, F. M. et al. Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis. PEERJ, v. 4, p. e1734, 2016. Disponível em: <https://peerj.com/articles/1734/#additional-information>. Acesso em: 16 jun. 2016. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/6629 |
dc.identifier.issn.none.fl_str_mv |
2167-9843 |
dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.7717/peerj.1734/supp-1 |
identifier_str_mv |
FERREIRA, F. M. et al. Unravelling potential virulence factor candidates in Xanthomonas citri. subsp. citri by secretome analysis. PEERJ, v. 4, p. e1734, 2016. Disponível em: <https://peerj.com/articles/1734/#additional-information>. Acesso em: 16 jun. 2016. 2167-9843 |
url |
http://www.repositorio.ufop.br/handle/123456789/6629 https://doi.org/10.7717/peerj.1734/supp-1 |
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eng |
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