Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFPA |
Texto Completo: | http://repositorio.ufpa.br/jspui/handle/2011/7035 |
Resumo: | Paracoccidioidomycosis is diagnosed from the direct observation of the causative agent, but serology can facilitate and decrease the time required for diagnosis. The objective of this study was to determine the influence of serum sample inactivation on the performance of the latex agglutination test (LAT) for detecting antibodies against Paracoccidioides brasiliensis. The sensitivity of LAT from inactivated or non-inactivated samples was 73% and 83%, respectively and the LAT selectivity was 79% and 90%, respectively. The LAT evaluated here was no more specific than the double-immunodiffusion assay. We suggest the investigation of other methods for improving the LAT, such as the use of deglycosylated antigen. |
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2015-11-30T13:55:23Z2015-11-30T13:55:23Z2012-06GOMES, Fabíola Silveira; SILVA, Silvia Helena Marques da. Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 107, n. 4, p. 510-512, jun. 2012. Disponível em: <http://www.scielo.br/pdf/mioc/v107n4/11.pdf>. Acesso em: 16 nov. 2015. <http://dx.doi.org/10.1590/S0074-02762012000400011>.0074-0276http://repositorio.ufpa.br/jspui/handle/2011/7035Paracoccidioidomycosis is diagnosed from the direct observation of the causative agent, but serology can facilitate and decrease the time required for diagnosis. The objective of this study was to determine the influence of serum sample inactivation on the performance of the latex agglutination test (LAT) for detecting antibodies against Paracoccidioides brasiliensis. The sensitivity of LAT from inactivated or non-inactivated samples was 73% and 83%, respectively and the LAT selectivity was 79% and 90%, respectively. The LAT evaluated here was no more specific than the double-immunodiffusion assay. We suggest the investigation of other methods for improving the LAT, such as the use of deglycosylated antigen.engParacoccidioidomicoseDiagnóstico sorológicoTeste de aglutinação em latexEffect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGOMES, Fabíola SilveiraSILVA, Silvia Helena Marques dainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPAinstname:Universidade Federal do Pará (UFPA)instacron:UFPAORIGINALArtigo_EffectSerumSample.pdfArtigo_EffectSerumSample.pdfapplication/pdf496399http://repositorio.ufpa.br/oai/bitstream/2011/7035/1/Artigo_EffectSerumSample.pdf3fab22aebde0fa276d7eae2879b4ba40MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://repositorio.ufpa.br/oai/bitstream/2011/7035/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-821468http://repositorio.ufpa.br/oai/bitstream/2011/7035/3/license_textb726c2dea4df03a814591001196bb5c7MD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-822190http://repositorio.ufpa.br/oai/bitstream/2011/7035/4/license_rdf19e8a2b57ef43c09f4d7071d2153c97dMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81703http://repositorio.ufpa.br/oai/bitstream/2011/7035/5/license.txta12ee01655d4f43dacf016d5e6168febMD55TEXTArtigo_EffectSerumSample.pdf.txtArtigo_EffectSerumSample.pdf.txtExtracted texttext/plain14173http://repositorio.ufpa.br/oai/bitstream/2011/7035/6/Artigo_EffectSerumSample.pdf.txt0c888699896366ea73c7164caf371006MD562011/70352017-12-14 10:26:26.053oai:repositorio.ufpa.br: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Repositório InstitucionalPUBhttp://repositorio.ufpa.br/oai/requestriufpabc@ufpa.bropendoar:21232017-12-14T13:26:26Repositório Institucional da UFPA - Universidade Federal do Pará (UFPA)false |
dc.title.pt_BR.fl_str_mv |
Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis |
title |
Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis |
spellingShingle |
Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis GOMES, Fabíola Silveira Paracoccidioidomicose Diagnóstico sorológico Teste de aglutinação em latex |
title_short |
Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis |
title_full |
Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis |
title_fullStr |
Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis |
title_full_unstemmed |
Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis |
title_sort |
Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis |
author |
GOMES, Fabíola Silveira |
author_facet |
GOMES, Fabíola Silveira SILVA, Silvia Helena Marques da |
author_role |
author |
author2 |
SILVA, Silvia Helena Marques da |
author2_role |
author |
dc.contributor.author.fl_str_mv |
GOMES, Fabíola Silveira SILVA, Silvia Helena Marques da |
dc.subject.por.fl_str_mv |
Paracoccidioidomicose Diagnóstico sorológico Teste de aglutinação em latex |
topic |
Paracoccidioidomicose Diagnóstico sorológico Teste de aglutinação em latex |
description |
Paracoccidioidomycosis is diagnosed from the direct observation of the causative agent, but serology can facilitate and decrease the time required for diagnosis. The objective of this study was to determine the influence of serum sample inactivation on the performance of the latex agglutination test (LAT) for detecting antibodies against Paracoccidioides brasiliensis. The sensitivity of LAT from inactivated or non-inactivated samples was 73% and 83%, respectively and the LAT selectivity was 79% and 90%, respectively. The LAT evaluated here was no more specific than the double-immunodiffusion assay. We suggest the investigation of other methods for improving the LAT, such as the use of deglycosylated antigen. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-06 |
dc.date.accessioned.fl_str_mv |
2015-11-30T13:55:23Z |
dc.date.available.fl_str_mv |
2015-11-30T13:55:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
GOMES, Fabíola Silveira; SILVA, Silvia Helena Marques da. Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 107, n. 4, p. 510-512, jun. 2012. Disponível em: <http://www.scielo.br/pdf/mioc/v107n4/11.pdf>. Acesso em: 16 nov. 2015. <http://dx.doi.org/10.1590/S0074-02762012000400011>. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpa.br/jspui/handle/2011/7035 |
dc.identifier.issn.none.fl_str_mv |
0074-0276 |
identifier_str_mv |
GOMES, Fabíola Silveira; SILVA, Silvia Helena Marques da. Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 107, n. 4, p. 510-512, jun. 2012. Disponível em: <http://www.scielo.br/pdf/mioc/v107n4/11.pdf>. Acesso em: 16 nov. 2015. <http://dx.doi.org/10.1590/S0074-02762012000400011>. 0074-0276 |
url |
http://repositorio.ufpa.br/jspui/handle/2011/7035 |
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eng |
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