Cultura de anteras e de embriões zigóticos imaturos no melhoramento de pimenteiras ornamentais (Capsicum annuum L.)
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFPB |
Texto Completo: | https://repositorio.ufpb.br/jspui/handle/123456789/14542 |
Resumo: | The pepper cultivates in pots have stood out in the ornamental market for its aesthetic features that include, among other attributes, the fruits and leaves color and harmony with the pots. The ornamental pepper breeding has been carried out over the long-term, involving multiple cycles of selfing. Biotechnological techniques, such as the culture of anther and immature zygotic embryos, can assist breeding programs reducing the time for the release of new cultivars. Thus, the objective was to induce androgenics embryos from anthers of different genotypes of ornamental pepper and regenerate viable plants from in vitro culture of immature zygotic embryos in different genotypes of ornamental pepper (Capsicum annuum L.). For anther culture technique were used anther of nine parents and five hybrids. These were maintained at 4°C for 3 days and subsequently cultured in double layer, with and without growth regulator and maintained in B.O.D. at 9 ° C for one week and then at 28° C in the absence of light. The cultivation of zygotic embryos, fruit of three genotypes were collected 20, 25, 30 and 35 days after selfing (DAA). Fruits were characterized and determined the embryo stage of development. The embryos were inoculated in the petri dishes containing the media: ½ MS and MS overall strength. It was recorded the number of embryos germinated and losses during acclimatization and transplantation, also the characterization of seedlings and plants in a greenhouse. In addition, it was determined breeding cycle plant of immature embryos and by the conventional method. There was influence of the media and genotypes in the induction of androgenic embryos. Fully developed embryos were observed in genotypes UFPB 001, UFPB 004 and UFPB 099 but a lot of abnormal embryos was recorded. Zygotic embryos at different developmental stages can be found in the same fruit characterizing the asynchronous development. Lower rates of germination and development of seedlings were observed in earlier fruit, being observed also influences the genotype. The interaction between the periods, genotypes and media influenced the morphological characterization of the plants in the greenhouse. On average a reduction of 30% was observed in the breeding cycle in the three genotypes. Further studies should be conducted to increase the conversion of normal androgenic embryos and in the case of zygotic embryos, increase germination rates in periods of 20 and 25 DAA and reduce losses during acclimatization and transplantation. Still, a considerable reduction in cycle improvement was achieved as well as the identification of genotypes responsive to anther culture. Thus, it will be a valuable tool for ornamental breeding program. |
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Cultura de anteras e de embriões zigóticos imaturos no melhoramento de pimenteiras ornamentais (Capsicum annuum L.)Capsicum annuumCultura de tecidosAndrogêneseEmbriogênese zigóticaCNPQ::CIENCIAS AGRARIAS::AGRONOMIAThe pepper cultivates in pots have stood out in the ornamental market for its aesthetic features that include, among other attributes, the fruits and leaves color and harmony with the pots. The ornamental pepper breeding has been carried out over the long-term, involving multiple cycles of selfing. Biotechnological techniques, such as the culture of anther and immature zygotic embryos, can assist breeding programs reducing the time for the release of new cultivars. Thus, the objective was to induce androgenics embryos from anthers of different genotypes of ornamental pepper and regenerate viable plants from in vitro culture of immature zygotic embryos in different genotypes of ornamental pepper (Capsicum annuum L.). For anther culture technique were used anther of nine parents and five hybrids. These were maintained at 4°C for 3 days and subsequently cultured in double layer, with and without growth regulator and maintained in B.O.D. at 9 ° C for one week and then at 28° C in the absence of light. The cultivation of zygotic embryos, fruit of three genotypes were collected 20, 25, 30 and 35 days after selfing (DAA). Fruits were characterized and determined the embryo stage of development. The embryos were inoculated in the petri dishes containing the media: ½ MS and MS overall strength. It was recorded the number of embryos germinated and losses during acclimatization and transplantation, also the characterization of seedlings and plants in a greenhouse. In addition, it was determined breeding cycle plant of immature embryos and by the conventional method. There was influence of the media and genotypes in the induction of androgenic embryos. Fully developed embryos were observed in genotypes UFPB 001, UFPB 004 and UFPB 099 but a lot of abnormal embryos was recorded. Zygotic embryos at different developmental stages can be found in the same fruit characterizing the asynchronous development. Lower rates of germination and development of seedlings were observed in earlier fruit, being observed also influences the genotype. The interaction between the periods, genotypes and media influenced the morphological characterization of the plants in the greenhouse. On average a reduction of 30% was observed in the breeding cycle in the three genotypes. Further studies should be conducted to increase the conversion of normal androgenic embryos and in the case of zygotic embryos, increase germination rates in periods of 20 and 25 DAA and reduce losses during acclimatization and transplantation. Still, a considerable reduction in cycle improvement was achieved as well as the identification of genotypes responsive to anther culture. Thus, it will be a valuable tool for ornamental breeding program.As pimenteiras cultivadas em vaso têm se destacado no mercado de ornamentais por suas características estéticas que envolvem, entre outros atributos, a coloração do frutos e folhas e harmonia com o vaso. O melhoramento de pimenteiras ornamentais tem sido realizado a longo prazo, envolvendo vários ciclos de autofecundação. Técnicas biotecnológicas, como a cultura de anteras e de embriões zigóticos imaturos, podem auxiliar os programas de melhoramento reduzindo o tempo para o lançamento das novas cultivares. Desta forma, o objetivo deste trabalho foi induzir embriões androgênicos a partir de anteras de diferentes genótipos de pimenteiras ornamentais e regenerar plantas viáveis a partir do cultivo in vitro de embriões zigóticos imaturos nos diferentes genótipos de pimenterias ornamentais (Capsicum annuum L.). Para a técnica de cultura de anteras, foram utilizadas anteras de nove genitores e cinco hibridos. Estas, foram mantidas a 4ºC por 3 dias e posteriormente cultivadas em meio dupla camada, com e sem regulador de crescimento e mantidas em B.O.D. a 9ºC por uma semana e posteriormente a 28ºC na ausência de luz. No cultivo de embriões zigóticos, foram coletados frutos de três genótipos com 20, 25, 30 e 35 dias após a autofecundação (DAA). Os frutos foram caraterizados e determinados o estádio de desenvolvimento do embrião. Os embriões foram inoculados em discos de Petri contendo os meios: MS ½ e MS força total. Foi determinado o número de embriões germinados e as perdas durante a aclimatização e transplantio, realizada também a caracterização das plântulas e das plantas em casa de vegetação. Além disso, foi determinado o ciclo de melhoramento das plantas obtidas por embriões imaturos e pelo método convencional. Houve influência dos meios e genótipos na indução de embriões androgênicos. Embriões totalmente desenvolvidos foram observados nos genótipos UFPB 001, UFPB 004 e UFPB 099 porém, uma grande quantidade de embriões anormais foi observada. Embriões zigóticos em diferentes estádios de desenvolvimento podem ser encontrados no mesmo fruto, caracterizando o desenvovlimento assincronico. Menores taxas de germinação e desenvolvimento das plântulas foram observadas em frutos mais precoces, sendo observada também influência do genótipo. A interação entre os períodos, genótipos e meios influenciaram a caracterização morfológica das plantas em casa de vegetação. Foi observada uma redução de em média 30% no ciclo de melhoramento, nos três genótipos. Estudos mais aprofundados devem ser realizados buscando aumentar a conversão em embriões androgênicos normais e no caso dos embriões zigóticos, aumentar as taxas de germinação nos períodos de 20 e 25 DAA e reduzir as perdas durante a aclimatização e transplantio. Ainda assim, uma redução considerável no ciclo de melhoramento foi conseguida, como também a identificação de genótipos responsivos a cultura de anteras. Sendo assim, será uma ferramenta valiosa para o programa de melhoramento de ornamentais.Universidade Federal da ParaíbaBrasilCiências Fitotecnia e Ciências AmbientaisPrograma de Pós-Graduação em AgronomiaUFPBRêgo, Mailson Monteiro dohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795039T5Rêgo, Elizanilda Ramalho dohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795039Y6Barroso, Priscila Alves2019-06-04T10:45:24Z2016-07-152019-06-04T10:45:24Z2016-02-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesishttps://repositorio.ufpb.br/jspui/handle/123456789/14542porAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFPBinstname:Universidade Federal da Paraíba (UFPB)instacron:UFPB2019-06-05T06:05:03Zoai:repositorio.ufpb.br:123456789/14542Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufpb.br/PUBhttp://tede.biblioteca.ufpb.br:8080/oai/requestdiretoria@ufpb.br|| diretoria@ufpb.bropendoar:2019-06-05T06:05:03Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)false |
dc.title.none.fl_str_mv |
Cultura de anteras e de embriões zigóticos imaturos no melhoramento de pimenteiras ornamentais (Capsicum annuum L.) |
title |
Cultura de anteras e de embriões zigóticos imaturos no melhoramento de pimenteiras ornamentais (Capsicum annuum L.) |
spellingShingle |
Cultura de anteras e de embriões zigóticos imaturos no melhoramento de pimenteiras ornamentais (Capsicum annuum L.) Barroso, Priscila Alves Capsicum annuum Cultura de tecidos Androgênese Embriogênese zigótica CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Cultura de anteras e de embriões zigóticos imaturos no melhoramento de pimenteiras ornamentais (Capsicum annuum L.) |
title_full |
Cultura de anteras e de embriões zigóticos imaturos no melhoramento de pimenteiras ornamentais (Capsicum annuum L.) |
title_fullStr |
Cultura de anteras e de embriões zigóticos imaturos no melhoramento de pimenteiras ornamentais (Capsicum annuum L.) |
title_full_unstemmed |
Cultura de anteras e de embriões zigóticos imaturos no melhoramento de pimenteiras ornamentais (Capsicum annuum L.) |
title_sort |
Cultura de anteras e de embriões zigóticos imaturos no melhoramento de pimenteiras ornamentais (Capsicum annuum L.) |
author |
Barroso, Priscila Alves |
author_facet |
Barroso, Priscila Alves |
author_role |
author |
dc.contributor.none.fl_str_mv |
Rêgo, Mailson Monteiro do http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795039T5 Rêgo, Elizanilda Ramalho do http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795039Y6 |
dc.contributor.author.fl_str_mv |
Barroso, Priscila Alves |
dc.subject.por.fl_str_mv |
Capsicum annuum Cultura de tecidos Androgênese Embriogênese zigótica CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
topic |
Capsicum annuum Cultura de tecidos Androgênese Embriogênese zigótica CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
The pepper cultivates in pots have stood out in the ornamental market for its aesthetic features that include, among other attributes, the fruits and leaves color and harmony with the pots. The ornamental pepper breeding has been carried out over the long-term, involving multiple cycles of selfing. Biotechnological techniques, such as the culture of anther and immature zygotic embryos, can assist breeding programs reducing the time for the release of new cultivars. Thus, the objective was to induce androgenics embryos from anthers of different genotypes of ornamental pepper and regenerate viable plants from in vitro culture of immature zygotic embryos in different genotypes of ornamental pepper (Capsicum annuum L.). For anther culture technique were used anther of nine parents and five hybrids. These were maintained at 4°C for 3 days and subsequently cultured in double layer, with and without growth regulator and maintained in B.O.D. at 9 ° C for one week and then at 28° C in the absence of light. The cultivation of zygotic embryos, fruit of three genotypes were collected 20, 25, 30 and 35 days after selfing (DAA). Fruits were characterized and determined the embryo stage of development. The embryos were inoculated in the petri dishes containing the media: ½ MS and MS overall strength. It was recorded the number of embryos germinated and losses during acclimatization and transplantation, also the characterization of seedlings and plants in a greenhouse. In addition, it was determined breeding cycle plant of immature embryos and by the conventional method. There was influence of the media and genotypes in the induction of androgenic embryos. Fully developed embryos were observed in genotypes UFPB 001, UFPB 004 and UFPB 099 but a lot of abnormal embryos was recorded. Zygotic embryos at different developmental stages can be found in the same fruit characterizing the asynchronous development. Lower rates of germination and development of seedlings were observed in earlier fruit, being observed also influences the genotype. The interaction between the periods, genotypes and media influenced the morphological characterization of the plants in the greenhouse. On average a reduction of 30% was observed in the breeding cycle in the three genotypes. Further studies should be conducted to increase the conversion of normal androgenic embryos and in the case of zygotic embryos, increase germination rates in periods of 20 and 25 DAA and reduce losses during acclimatization and transplantation. Still, a considerable reduction in cycle improvement was achieved as well as the identification of genotypes responsive to anther culture. Thus, it will be a valuable tool for ornamental breeding program. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07-15 2016-02-12 2019-06-04T10:45:24Z 2019-06-04T10:45:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufpb.br/jspui/handle/123456789/14542 |
url |
https://repositorio.ufpb.br/jspui/handle/123456789/14542 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal da Paraíba Brasil Ciências Fitotecnia e Ciências Ambientais Programa de Pós-Graduação em Agronomia UFPB |
publisher.none.fl_str_mv |
Universidade Federal da Paraíba Brasil Ciências Fitotecnia e Ciências Ambientais Programa de Pós-Graduação em Agronomia UFPB |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UFPB instname:Universidade Federal da Paraíba (UFPB) instacron:UFPB |
instname_str |
Universidade Federal da Paraíba (UFPB) |
instacron_str |
UFPB |
institution |
UFPB |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UFPB |
collection |
Biblioteca Digital de Teses e Dissertações da UFPB |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB) |
repository.mail.fl_str_mv |
diretoria@ufpb.br|| diretoria@ufpb.br |
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1801842948427481088 |