Potencial proliferativo da Acetilcolina em cardiomiócitos

Detalhes bibliográficos
Autor(a) principal: Grisi, Yasmim Estrela Batista
Data de Publicação: 2019
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFPB
Texto Completo: https://repositorio.ufpb.br/jspui/handle/123456789/18609
Resumo: Cardiovascular diseases continue to be the biggest causes of death in the world for more than a decade, and Acute Myocardial Infarction (AMI) is the most frequently caused by diseases in Brazil. The damage caused by MI is caused by loss of functionality, local inflammatory process, ventricular remodeling, cystic fibrosis, which can culminate in heart failure until death. Strategies to revert this situation are studied and present several lines, and the most discrepant point indicates to be a cardiac regeneration. Acetylcholine (ACh) was subjected to a molecule with cardioprotective, anti-inflammatory effects and that cardiomyocytes have independent machines for the production of acetylcholine, was the main objective of studying whether an ACh has a proliferative role in cardiomyocytes after treatment with pyridostigmine. Observe the behavior in cardiomyocyte cultures of newborn rats from 3 to 5 days, when used with pyridostigmine (PIR), which is an inhibitor of acetylcholinesterase (enzyme that degraded ACh) and keep it modulated in these genes participate in the cell cycle. The chosen genes were Aurora BK, CDK1 and CCND2, data that were analyzed after an RT-PCR and compared with a B-ACTINA normalizer. It was concluded that the results show an ACh proliferative potential when PIR, in 0.1mM and 1mM, in the cardiomyocytes of neonatal rats, modulation of some genes in the cell cycle and potential in the application of treatment in AMI in neonatal rats.
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spelling Potencial proliferativo da Acetilcolina em cardiomiócitosInfarto do miocárdioAcetilcolinaProliferaçãoMyocardial infarctionAcetylcholineProliferationCNPQ::CIENCIAS BIOLOGICASCardiovascular diseases continue to be the biggest causes of death in the world for more than a decade, and Acute Myocardial Infarction (AMI) is the most frequently caused by diseases in Brazil. The damage caused by MI is caused by loss of functionality, local inflammatory process, ventricular remodeling, cystic fibrosis, which can culminate in heart failure until death. Strategies to revert this situation are studied and present several lines, and the most discrepant point indicates to be a cardiac regeneration. Acetylcholine (ACh) was subjected to a molecule with cardioprotective, anti-inflammatory effects and that cardiomyocytes have independent machines for the production of acetylcholine, was the main objective of studying whether an ACh has a proliferative role in cardiomyocytes after treatment with pyridostigmine. Observe the behavior in cardiomyocyte cultures of newborn rats from 3 to 5 days, when used with pyridostigmine (PIR), which is an inhibitor of acetylcholinesterase (enzyme that degraded ACh) and keep it modulated in these genes participate in the cell cycle. The chosen genes were Aurora BK, CDK1 and CCND2, data that were analyzed after an RT-PCR and compared with a B-ACTINA normalizer. It was concluded that the results show an ACh proliferative potential when PIR, in 0.1mM and 1mM, in the cardiomyocytes of neonatal rats, modulation of some genes in the cell cycle and potential in the application of treatment in AMI in neonatal rats.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESDoenças Cardiovasculares continuam a ser as maiores causas de morte em todo o mundo a mais de uma década, e o Infarto Agudo do miocárdio (IAM) é a mais recorrente dessas doenças, no Brasil. Os danos causados pelo IM são desde uma perda de funcionalidade, processo inflamatório local, remodelamento ventricular, fibrose cística, podendo culminar numa insuficiência cardíaca até a morte. Estratégias para reverter esse quadro são estudadas e propostas em várias linhas, e o ponto alvo mais discrepante aponta ser a regeneração cardíaca. Considerando que a Acetilcolina (ACh) tem sido apresentada como uma molécula com efeitos cardioprotetores, antiinflamatórios e que os cardiomiócitos possuem maquinaria, independente, para a produção da acetilcolina, foi objetivo desse estudo observar se a ACh tem papel proliferativo nos cardiomiócitos a partir do tratamento com Piridostigmina. Observou-se o comportamento em culturas de cardiomiócitos de ratos neonatos de 3 a 5 dias, quando tratados com a Piridostigmina (PIR), que é uma inibidora de acetilcolinesterase (enzima que degrada a ACh), e observou-se a modulação desta em genes envolvidos no ciclo celular. Os genes escolhidos foram Aurora BK, CDK1 e CCND2, dados que foram analisados após uma RT-PCR, e comparados com um normalizador B-ACTINA. Concluiu-se que os achados experimentais mostraram potencial proliferativo da ACh quando tratados com PIR, em concentrações de 0,1mM e 1mM, nos cardiomiócitos de ratos neonatos, modulação de alguns genes no ciclo celular, e potencial na aplicação de tratamento ao IAM em ratos neonatos.Universidade Federal da ParaíbaBrasilBiotecnologiaPrograma de Pós-Graduação em BiotecnologiaUFPBGomes, Enéas Ricardo de Morais Gomeshttp://lattes.cnpq.br/2707212883421135Grisi, Yasmim Estrela Batista2020-12-06T21:26:03Z2020-11-302020-12-06T21:26:03Z2019-11-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttps://repositorio.ufpb.br/jspui/handle/123456789/18609porhttp://creativecommons.org/licenses/by-nd/3.0/br/info:eu-repo/semantics/embargoedAccessreponame:Biblioteca Digital de Teses e Dissertações da UFPBinstname:Universidade Federal da Paraíba (UFPB)instacron:UFPB2021-09-08T13:56:20Zoai:repositorio.ufpb.br:123456789/18609Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufpb.br/PUBhttp://tede.biblioteca.ufpb.br:8080/oai/requestdiretoria@ufpb.br|| diretoria@ufpb.bropendoar:2021-09-08T13:56:20Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)false
dc.title.none.fl_str_mv Potencial proliferativo da Acetilcolina em cardiomiócitos
title Potencial proliferativo da Acetilcolina em cardiomiócitos
spellingShingle Potencial proliferativo da Acetilcolina em cardiomiócitos
Grisi, Yasmim Estrela Batista
Infarto do miocárdio
Acetilcolina
Proliferação
Myocardial infarction
Acetylcholine
Proliferation
CNPQ::CIENCIAS BIOLOGICAS
title_short Potencial proliferativo da Acetilcolina em cardiomiócitos
title_full Potencial proliferativo da Acetilcolina em cardiomiócitos
title_fullStr Potencial proliferativo da Acetilcolina em cardiomiócitos
title_full_unstemmed Potencial proliferativo da Acetilcolina em cardiomiócitos
title_sort Potencial proliferativo da Acetilcolina em cardiomiócitos
author Grisi, Yasmim Estrela Batista
author_facet Grisi, Yasmim Estrela Batista
author_role author
dc.contributor.none.fl_str_mv Gomes, Enéas Ricardo de Morais Gomes
http://lattes.cnpq.br/2707212883421135
dc.contributor.author.fl_str_mv Grisi, Yasmim Estrela Batista
dc.subject.por.fl_str_mv Infarto do miocárdio
Acetilcolina
Proliferação
Myocardial infarction
Acetylcholine
Proliferation
CNPQ::CIENCIAS BIOLOGICAS
topic Infarto do miocárdio
Acetilcolina
Proliferação
Myocardial infarction
Acetylcholine
Proliferation
CNPQ::CIENCIAS BIOLOGICAS
description Cardiovascular diseases continue to be the biggest causes of death in the world for more than a decade, and Acute Myocardial Infarction (AMI) is the most frequently caused by diseases in Brazil. The damage caused by MI is caused by loss of functionality, local inflammatory process, ventricular remodeling, cystic fibrosis, which can culminate in heart failure until death. Strategies to revert this situation are studied and present several lines, and the most discrepant point indicates to be a cardiac regeneration. Acetylcholine (ACh) was subjected to a molecule with cardioprotective, anti-inflammatory effects and that cardiomyocytes have independent machines for the production of acetylcholine, was the main objective of studying whether an ACh has a proliferative role in cardiomyocytes after treatment with pyridostigmine. Observe the behavior in cardiomyocyte cultures of newborn rats from 3 to 5 days, when used with pyridostigmine (PIR), which is an inhibitor of acetylcholinesterase (enzyme that degraded ACh) and keep it modulated in these genes participate in the cell cycle. The chosen genes were Aurora BK, CDK1 and CCND2, data that were analyzed after an RT-PCR and compared with a B-ACTINA normalizer. It was concluded that the results show an ACh proliferative potential when PIR, in 0.1mM and 1mM, in the cardiomyocytes of neonatal rats, modulation of some genes in the cell cycle and potential in the application of treatment in AMI in neonatal rats.
publishDate 2019
dc.date.none.fl_str_mv 2019-11-30
2020-12-06T21:26:03Z
2020-11-30
2020-12-06T21:26:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.ufpb.br/jspui/handle/123456789/18609
url https://repositorio.ufpb.br/jspui/handle/123456789/18609
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nd/3.0/br/
info:eu-repo/semantics/embargoedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nd/3.0/br/
eu_rights_str_mv embargoedAccess
dc.publisher.none.fl_str_mv Universidade Federal da Paraíba
Brasil
Biotecnologia
Programa de Pós-Graduação em Biotecnologia
UFPB
publisher.none.fl_str_mv Universidade Federal da Paraíba
Brasil
Biotecnologia
Programa de Pós-Graduação em Biotecnologia
UFPB
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFPB
instname:Universidade Federal da Paraíba (UFPB)
instacron:UFPB
instname_str Universidade Federal da Paraíba (UFPB)
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institution UFPB
reponame_str Biblioteca Digital de Teses e Dissertações da UFPB
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repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)
repository.mail.fl_str_mv diretoria@ufpb.br|| diretoria@ufpb.br
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