Citogenética de Santalales: Heterocromatina e Quantificação de DNA

Detalhes bibliográficos
Autor(a) principal: Neves, Jose Achilles de Lima
Data de Publicação: 2022
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFPB
Texto Completo: https://repositorio.ufpb.br/jspui/handle/123456789/29658
Resumo: Santalales is the largest order among the 12 orders of angiosperms with parasitic species, distributed in tropical and temperate regions, absent only in Antarctica. Ximeniaceae, Loranthaceae, Santalaceae and Viscaceae are four of the 20 families of the order Santalales, consisting of hemiparasitic plants with roots or branches and primary and/or secondary haustoria, characterized by presenting generally large chromosomes, stable chromosome numbers and large genomes. In this work we aim to: (1) Report numerical chromosome variation in representatives of Santalales, particularly Loranthaceae and Viscaceae, since cytological studies are mainly based on meiotic analyses; and (2) Understand the mechanisms acting in the karyotypic evolution of this hemiparasitic group of plants. For that, staining with DAPI was used, as well as the double CMA/DAPI and quantification of nuclear DNA through flow cytometry. All analyzed species presented reticulated interphase nuclei, with 2n = 16, 18 and 28 for Loranthaceae, 2n = 16 and 28 for Viscaceae, 2n = 28 for Santalaceae and 2n = 24 for Ximeniaceae. The largest chromosomes were observed for Phoradendron and Psittacanthus, and the smallest ones for Ximenia, Dendrophthoe and Santalum. CMA/DAPI banding technique revealed the presence of terminal CMA+ bands for Ximeniaceae, interstitial, subterminal and terminal CMA+ bands in Loranthaceae, while in representatives of Santalaceae and Viscaceae were observed interstitial and pericentromeric CMA+ bands. The size of the genomes varied eightfold in Loranthaceae and sevenfold in Viscaceae. Loranthaceae presented, on average, smaller genomes (2C = 54,62 pg) compared to Viscaceae (2C = 74,64 pg). The chromosome numbers of main genera in Loranthaceae and Viscaceae were numerically variable suggesting dysploidy and polyploidy events as important mechanisms on the evolution of these plant groups. Among the analyzed species of the order Santalales, it was not possible to correlate number, mean chromosome size and nuclear DNA content. As well as the frequency of polyploidy in Viscaceae and Santalaceae, a combined analysis of number, mean chromosome size and nuclear DNA content in the same populations is necessary. Obtain these data is particularly difficult due to technical problems related to chromosome analysis in plant members with parasitic habits.
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spelling Citogenética de Santalales: Heterocromatina e Quantificação de DNAbandeamento cromossômicocitometria de fluxogenomas giganteshemiparasitasSantalalesCNPQ::CIENCIAS AGRARIASSantalales is the largest order among the 12 orders of angiosperms with parasitic species, distributed in tropical and temperate regions, absent only in Antarctica. Ximeniaceae, Loranthaceae, Santalaceae and Viscaceae are four of the 20 families of the order Santalales, consisting of hemiparasitic plants with roots or branches and primary and/or secondary haustoria, characterized by presenting generally large chromosomes, stable chromosome numbers and large genomes. In this work we aim to: (1) Report numerical chromosome variation in representatives of Santalales, particularly Loranthaceae and Viscaceae, since cytological studies are mainly based on meiotic analyses; and (2) Understand the mechanisms acting in the karyotypic evolution of this hemiparasitic group of plants. For that, staining with DAPI was used, as well as the double CMA/DAPI and quantification of nuclear DNA through flow cytometry. All analyzed species presented reticulated interphase nuclei, with 2n = 16, 18 and 28 for Loranthaceae, 2n = 16 and 28 for Viscaceae, 2n = 28 for Santalaceae and 2n = 24 for Ximeniaceae. The largest chromosomes were observed for Phoradendron and Psittacanthus, and the smallest ones for Ximenia, Dendrophthoe and Santalum. CMA/DAPI banding technique revealed the presence of terminal CMA+ bands for Ximeniaceae, interstitial, subterminal and terminal CMA+ bands in Loranthaceae, while in representatives of Santalaceae and Viscaceae were observed interstitial and pericentromeric CMA+ bands. The size of the genomes varied eightfold in Loranthaceae and sevenfold in Viscaceae. Loranthaceae presented, on average, smaller genomes (2C = 54,62 pg) compared to Viscaceae (2C = 74,64 pg). The chromosome numbers of main genera in Loranthaceae and Viscaceae were numerically variable suggesting dysploidy and polyploidy events as important mechanisms on the evolution of these plant groups. Among the analyzed species of the order Santalales, it was not possible to correlate number, mean chromosome size and nuclear DNA content. As well as the frequency of polyploidy in Viscaceae and Santalaceae, a combined analysis of number, mean chromosome size and nuclear DNA content in the same populations is necessary. Obtain these data is particularly difficult due to technical problems related to chromosome analysis in plant members with parasitic habits.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESSantalales é a maior ordem entre as 12 ordens de angiospermas com espécies parasitas, distribuída em regiões tropicais e temperadas, ausente apenas na Antártida. Ximeniaceae, Loranthaceae, Santalaceae e Viscaceae são quatro das 20 famílias da ordem Santalales, constituídas por plantas hemiparasitas de raízes ou ramos com haustórios primários e/ou secundários, caracterizadas por apresentar geralmente cromossomos grandes, números cromossômicos estáveis e grandes genomas. Neste trabalho objetivamos: (1) Relatar a variação cromossômica numérica em representantes de Santalales, particularmente, Loranthaceae e Viscaceae, uma vez que os estudos citológicos, em sua maioria, são baseados principalmente em análises meióticas; (2) Compreender os mecanismos atuantes na evolução cariotípica desse grupo de plantas hemiparasitas. Para tanto, foi utilizado a coloração com DAPI, bem como, a dupla CMA/DAPI e quantificação do DNA nuclear através da citometria de fluxo. Todas as espécies analisadas apresentaram núcleos interfásicos reticulados, com 2n = 16, 18 e 28 para Loranthaceae, 2n = 16 e 28 para Viscaceae, 2n = 28 para Santalaceae e 2n = 24 para Ximeniaceae. Os maiores cromossomos foram observados para os gêneros Phoradendron e Psittacanthus, e os menores Ximenia, Dendrophthoe e Santalum. O bandeamento CMA/DAPI revelou a presença de bandas CMA+ terminais para Ximeniaceae, bandas CMA+ intersticiais, subterminais e terminais em Loranthaceae, enquanto que em representantes de Santalaceae e Viscaceae foram observadas bandas CMA+ intersticiais e pericentroméricas. O tamanho dos genomas teve uma variação de oito vezes em Loranthaceae, e de sete vezes em Viscaceae. Loranthaceae apresentou, em média, genomas menores (2C = 54,62 pg) em comparação a Viscaceae (2C = 74,64 pg). Os principais gêneros das famílias Loranthaceae e Viscaceae se mostraram numericamente variáveis com números cromossômicos sugerindo eventos de disploidia e poliploidia como mecanismos importantes na evolução desses grupos de plantas. Entre as espécies analisadas da ordem Santalales não foi possível correlacionar número, tamanho cromossômico médio e conteúdo de DNA nuclear. Assim como na frequência de poliploidia em Viscaceae e Santalaceae, é necessária uma análise conjunta de número, tamanho cromossômico médio e conteúdo de DNA nuclear nas mesmas populações. A obtenção desses dados se torna particularmente difícil pelos problemas técnicos relacionados às análises cromossômicas em membros de plantas com hábito parasítico.Universidade Federal da ParaíbaBrasilCiências BiológicasPrograma de Pós-Graduação em AgronomiaUFPBFelix, Leonardo Pessoahttp://lattes.cnpq.br/0180466204127182Batista, Fabiane Rabelo da Costahttp://lattes.cnpq.br/6343125757566396Neves, Jose Achilles de Lima2024-02-27T14:25:12Z2023-03-022024-02-27T14:25:12Z2022-02-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesishttps://repositorio.ufpb.br/jspui/handle/123456789/29658porinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFPBinstname:Universidade Federal da Paraíba (UFPB)instacron:UFPB2024-02-28T07:21:26Zoai:repositorio.ufpb.br:123456789/29658Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufpb.br/PUBhttp://tede.biblioteca.ufpb.br:8080/oai/requestdiretoria@ufpb.br|| diretoria@ufpb.bropendoar:2024-02-28T07:21:26Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)false
dc.title.none.fl_str_mv Citogenética de Santalales: Heterocromatina e Quantificação de DNA
title Citogenética de Santalales: Heterocromatina e Quantificação de DNA
spellingShingle Citogenética de Santalales: Heterocromatina e Quantificação de DNA
Neves, Jose Achilles de Lima
bandeamento cromossômico
citometria de fluxo
genomas gigantes
hemiparasitas
Santalales
CNPQ::CIENCIAS AGRARIAS
title_short Citogenética de Santalales: Heterocromatina e Quantificação de DNA
title_full Citogenética de Santalales: Heterocromatina e Quantificação de DNA
title_fullStr Citogenética de Santalales: Heterocromatina e Quantificação de DNA
title_full_unstemmed Citogenética de Santalales: Heterocromatina e Quantificação de DNA
title_sort Citogenética de Santalales: Heterocromatina e Quantificação de DNA
author Neves, Jose Achilles de Lima
author_facet Neves, Jose Achilles de Lima
author_role author
dc.contributor.none.fl_str_mv Felix, Leonardo Pessoa
http://lattes.cnpq.br/0180466204127182
Batista, Fabiane Rabelo da Costa
http://lattes.cnpq.br/6343125757566396
dc.contributor.author.fl_str_mv Neves, Jose Achilles de Lima
dc.subject.por.fl_str_mv bandeamento cromossômico
citometria de fluxo
genomas gigantes
hemiparasitas
Santalales
CNPQ::CIENCIAS AGRARIAS
topic bandeamento cromossômico
citometria de fluxo
genomas gigantes
hemiparasitas
Santalales
CNPQ::CIENCIAS AGRARIAS
description Santalales is the largest order among the 12 orders of angiosperms with parasitic species, distributed in tropical and temperate regions, absent only in Antarctica. Ximeniaceae, Loranthaceae, Santalaceae and Viscaceae are four of the 20 families of the order Santalales, consisting of hemiparasitic plants with roots or branches and primary and/or secondary haustoria, characterized by presenting generally large chromosomes, stable chromosome numbers and large genomes. In this work we aim to: (1) Report numerical chromosome variation in representatives of Santalales, particularly Loranthaceae and Viscaceae, since cytological studies are mainly based on meiotic analyses; and (2) Understand the mechanisms acting in the karyotypic evolution of this hemiparasitic group of plants. For that, staining with DAPI was used, as well as the double CMA/DAPI and quantification of nuclear DNA through flow cytometry. All analyzed species presented reticulated interphase nuclei, with 2n = 16, 18 and 28 for Loranthaceae, 2n = 16 and 28 for Viscaceae, 2n = 28 for Santalaceae and 2n = 24 for Ximeniaceae. The largest chromosomes were observed for Phoradendron and Psittacanthus, and the smallest ones for Ximenia, Dendrophthoe and Santalum. CMA/DAPI banding technique revealed the presence of terminal CMA+ bands for Ximeniaceae, interstitial, subterminal and terminal CMA+ bands in Loranthaceae, while in representatives of Santalaceae and Viscaceae were observed interstitial and pericentromeric CMA+ bands. The size of the genomes varied eightfold in Loranthaceae and sevenfold in Viscaceae. Loranthaceae presented, on average, smaller genomes (2C = 54,62 pg) compared to Viscaceae (2C = 74,64 pg). The chromosome numbers of main genera in Loranthaceae and Viscaceae were numerically variable suggesting dysploidy and polyploidy events as important mechanisms on the evolution of these plant groups. Among the analyzed species of the order Santalales, it was not possible to correlate number, mean chromosome size and nuclear DNA content. As well as the frequency of polyploidy in Viscaceae and Santalaceae, a combined analysis of number, mean chromosome size and nuclear DNA content in the same populations is necessary. Obtain these data is particularly difficult due to technical problems related to chromosome analysis in plant members with parasitic habits.
publishDate 2022
dc.date.none.fl_str_mv 2022-02-28
2023-03-02
2024-02-27T14:25:12Z
2024-02-27T14:25:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.ufpb.br/jspui/handle/123456789/29658
url https://repositorio.ufpb.br/jspui/handle/123456789/29658
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal da Paraíba
Brasil
Ciências Biológicas
Programa de Pós-Graduação em Agronomia
UFPB
publisher.none.fl_str_mv Universidade Federal da Paraíba
Brasil
Ciências Biológicas
Programa de Pós-Graduação em Agronomia
UFPB
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFPB
instname:Universidade Federal da Paraíba (UFPB)
instacron:UFPB
instname_str Universidade Federal da Paraíba (UFPB)
instacron_str UFPB
institution UFPB
reponame_str Biblioteca Digital de Teses e Dissertações da UFPB
collection Biblioteca Digital de Teses e Dissertações da UFPB
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)
repository.mail.fl_str_mv diretoria@ufpb.br|| diretoria@ufpb.br
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