Fecal bacterial communities of wild black capuchin monkeys (Sapajus nigritus) from the Atlantic Forest biome in Southern Brazil are divergent from those of other non-human primates
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/229497 |
Resumo: | Gut microbiota are influenced by factors such as diet, habitat, and social contact, which directly affect the host's health. Studies related to gut microbiota in non-human primates are increasing worldwide. However, little remains known about the gut bacterial composition in wild Brazilian monkeys. Therefore, we studied the fecal microbiota composition of wild black capuchin monkey (Sapajus nigritus) (n=10) populations from two different Atlantic Forest biome fragments (five individuals per fragment) in south Brazil. The bacterial community was identified via the high-throughput sequencing and partial amplification of the 16S rRNA gene (V4 region) using an Ion Personal Genome Machine (PGMTM) System. In contrast to other studies involving monkey microbiota, which have generally reported the phyla Firmicutes and Bacteroidetes as predominant, black capuchin monkeys showed a high relative abundance of Proteobacteria (X= 80.54%), followed by Firmicutes (X= 12.14%), Actinobacteria (X= 4.60%), and Bacteriodetes (X= 1.31%). This observed particularity may have been influenced by anthropogenic actions related to the wild habitat and/or diet specific to the Brazilian biome's characteristics and/or monkey foraging behavior. Comparisons of species richness (Chao1) and diversity indices (Simpson and InvSimpson) showed no significant differences between the two groups of monkeys. Interestingly, PICRUSt2 analysis revealed that metabolic pathways present in the bacterial communities were associated with xenobiotic biodegradation and the biosynthesis of secondary metabolites, which may suggest positive effects on monkey health and conservation in this anthropogenic habitat. Infectious disease-associated microorganisms were also observed in the samples. The present study provides information about the bacterial population and metabolic functions present in fecal microbiota, which may contribute to a better understanding of the ecology and biology of black capuchin monkeys living in forest fragments within the Atlantic Forest biome in southern Brazil. Additionally, the present study demonstrates that the fecal bacterial communities of wild black capuchin monkeys in this area are divergent from those of other wild non-human primates. |
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Grassotti, Tiela TrappKothe, Caroline IsabelPrichula, JaniraMohellibi, NacerMann, Michele BertoniWagner, Paulo Guilherme CarnielCampos, Fabrício SouzaCampos, Aline Alves ScarpelliniFrazzon, JeversonFrazzon, Ana Paula Guedes2021-09-03T04:23:28Z20212666-5174http://hdl.handle.net/10183/229497001130949Gut microbiota are influenced by factors such as diet, habitat, and social contact, which directly affect the host's health. Studies related to gut microbiota in non-human primates are increasing worldwide. However, little remains known about the gut bacterial composition in wild Brazilian monkeys. Therefore, we studied the fecal microbiota composition of wild black capuchin monkey (Sapajus nigritus) (n=10) populations from two different Atlantic Forest biome fragments (five individuals per fragment) in south Brazil. The bacterial community was identified via the high-throughput sequencing and partial amplification of the 16S rRNA gene (V4 region) using an Ion Personal Genome Machine (PGMTM) System. In contrast to other studies involving monkey microbiota, which have generally reported the phyla Firmicutes and Bacteroidetes as predominant, black capuchin monkeys showed a high relative abundance of Proteobacteria (X= 80.54%), followed by Firmicutes (X= 12.14%), Actinobacteria (X= 4.60%), and Bacteriodetes (X= 1.31%). This observed particularity may have been influenced by anthropogenic actions related to the wild habitat and/or diet specific to the Brazilian biome's characteristics and/or monkey foraging behavior. Comparisons of species richness (Chao1) and diversity indices (Simpson and InvSimpson) showed no significant differences between the two groups of monkeys. Interestingly, PICRUSt2 analysis revealed that metabolic pathways present in the bacterial communities were associated with xenobiotic biodegradation and the biosynthesis of secondary metabolites, which may suggest positive effects on monkey health and conservation in this anthropogenic habitat. Infectious disease-associated microorganisms were also observed in the samples. The present study provides information about the bacterial population and metabolic functions present in fecal microbiota, which may contribute to a better understanding of the ecology and biology of black capuchin monkeys living in forest fragments within the Atlantic Forest biome in southern Brazil. Additionally, the present study demonstrates that the fecal bacterial communities of wild black capuchin monkeys in this area are divergent from those of other wild non-human primates.application/pdfengCurrent Research in Microbial Sciences. Amsterdam. Vol. 2 (Dec. 2021), 100048, 14 p.Sapajus nigritusMicrobioma gastrointestinalEcossistemaFezesRobust capuchinsFecal microbiotaWild south Brazilian primatesPrimate conservationProteobacteriaFecal bacterial communities of wild black capuchin monkeys (Sapajus nigritus) from the Atlantic Forest biome in Southern Brazil are divergent from those of other non-human primatesEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001130949.pdf.txt001130949.pdf.txtExtracted Texttext/plain77735http://www.lume.ufrgs.br/bitstream/10183/229497/2/001130949.pdf.txt0ea7757d33b3b8cf19c857939992f7b0MD52ORIGINAL001130949.pdfTexto completo (inglês)application/pdf3395858http://www.lume.ufrgs.br/bitstream/10183/229497/1/001130949.pdfba7053048460c089139323c3eddc537fMD5110183/2294972021-09-19 04:25:57.023193oai:www.lume.ufrgs.br:10183/229497Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-09-19T07:25:57Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Fecal bacterial communities of wild black capuchin monkeys (Sapajus nigritus) from the Atlantic Forest biome in Southern Brazil are divergent from those of other non-human primates |
title |
Fecal bacterial communities of wild black capuchin monkeys (Sapajus nigritus) from the Atlantic Forest biome in Southern Brazil are divergent from those of other non-human primates |
spellingShingle |
Fecal bacterial communities of wild black capuchin monkeys (Sapajus nigritus) from the Atlantic Forest biome in Southern Brazil are divergent from those of other non-human primates Grassotti, Tiela Trapp Sapajus nigritus Microbioma gastrointestinal Ecossistema Fezes Robust capuchins Fecal microbiota Wild south Brazilian primates Primate conservation Proteobacteria |
title_short |
Fecal bacterial communities of wild black capuchin monkeys (Sapajus nigritus) from the Atlantic Forest biome in Southern Brazil are divergent from those of other non-human primates |
title_full |
Fecal bacterial communities of wild black capuchin monkeys (Sapajus nigritus) from the Atlantic Forest biome in Southern Brazil are divergent from those of other non-human primates |
title_fullStr |
Fecal bacterial communities of wild black capuchin monkeys (Sapajus nigritus) from the Atlantic Forest biome in Southern Brazil are divergent from those of other non-human primates |
title_full_unstemmed |
Fecal bacterial communities of wild black capuchin monkeys (Sapajus nigritus) from the Atlantic Forest biome in Southern Brazil are divergent from those of other non-human primates |
title_sort |
Fecal bacterial communities of wild black capuchin monkeys (Sapajus nigritus) from the Atlantic Forest biome in Southern Brazil are divergent from those of other non-human primates |
author |
Grassotti, Tiela Trapp |
author_facet |
Grassotti, Tiela Trapp Kothe, Caroline Isabel Prichula, Janira Mohellibi, Nacer Mann, Michele Bertoni Wagner, Paulo Guilherme Carniel Campos, Fabrício Souza Campos, Aline Alves Scarpellini Frazzon, Jeverson Frazzon, Ana Paula Guedes |
author_role |
author |
author2 |
Kothe, Caroline Isabel Prichula, Janira Mohellibi, Nacer Mann, Michele Bertoni Wagner, Paulo Guilherme Carniel Campos, Fabrício Souza Campos, Aline Alves Scarpellini Frazzon, Jeverson Frazzon, Ana Paula Guedes |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Grassotti, Tiela Trapp Kothe, Caroline Isabel Prichula, Janira Mohellibi, Nacer Mann, Michele Bertoni Wagner, Paulo Guilherme Carniel Campos, Fabrício Souza Campos, Aline Alves Scarpellini Frazzon, Jeverson Frazzon, Ana Paula Guedes |
dc.subject.por.fl_str_mv |
Sapajus nigritus Microbioma gastrointestinal Ecossistema Fezes |
topic |
Sapajus nigritus Microbioma gastrointestinal Ecossistema Fezes Robust capuchins Fecal microbiota Wild south Brazilian primates Primate conservation Proteobacteria |
dc.subject.eng.fl_str_mv |
Robust capuchins Fecal microbiota Wild south Brazilian primates Primate conservation Proteobacteria |
description |
Gut microbiota are influenced by factors such as diet, habitat, and social contact, which directly affect the host's health. Studies related to gut microbiota in non-human primates are increasing worldwide. However, little remains known about the gut bacterial composition in wild Brazilian monkeys. Therefore, we studied the fecal microbiota composition of wild black capuchin monkey (Sapajus nigritus) (n=10) populations from two different Atlantic Forest biome fragments (five individuals per fragment) in south Brazil. The bacterial community was identified via the high-throughput sequencing and partial amplification of the 16S rRNA gene (V4 region) using an Ion Personal Genome Machine (PGMTM) System. In contrast to other studies involving monkey microbiota, which have generally reported the phyla Firmicutes and Bacteroidetes as predominant, black capuchin monkeys showed a high relative abundance of Proteobacteria (X= 80.54%), followed by Firmicutes (X= 12.14%), Actinobacteria (X= 4.60%), and Bacteriodetes (X= 1.31%). This observed particularity may have been influenced by anthropogenic actions related to the wild habitat and/or diet specific to the Brazilian biome's characteristics and/or monkey foraging behavior. Comparisons of species richness (Chao1) and diversity indices (Simpson and InvSimpson) showed no significant differences between the two groups of monkeys. Interestingly, PICRUSt2 analysis revealed that metabolic pathways present in the bacterial communities were associated with xenobiotic biodegradation and the biosynthesis of secondary metabolites, which may suggest positive effects on monkey health and conservation in this anthropogenic habitat. Infectious disease-associated microorganisms were also observed in the samples. The present study provides information about the bacterial population and metabolic functions present in fecal microbiota, which may contribute to a better understanding of the ecology and biology of black capuchin monkeys living in forest fragments within the Atlantic Forest biome in southern Brazil. Additionally, the present study demonstrates that the fecal bacterial communities of wild black capuchin monkeys in this area are divergent from those of other wild non-human primates. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-09-03T04:23:28Z |
dc.date.issued.fl_str_mv |
2021 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/229497 |
dc.identifier.issn.pt_BR.fl_str_mv |
2666-5174 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001130949 |
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2666-5174 001130949 |
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http://hdl.handle.net/10183/229497 |
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eng |
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Current Research in Microbial Sciences. Amsterdam. Vol. 2 (Dec. 2021), 100048, 14 p. |
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openAccess |
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