Exercise alleviates hypoalgesia and increases the level of calcitonin gene-related peptide in the dorsal horn of the spinal cord of diabetic rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/108687 |
Resumo: | OBJECTIVE: The aim of this study was to evaluate the effects of treadmill training on nociceptive sensitivity and immunoreactivity to calcitonin gene-related peptide in the dorsal horn of the spinal cord of diabetic rats. METHODS: Male Wistar rats were divided into three groups: control, diabetic and trained diabetic. Treadmill training was performed for 8 weeks. The blood glucose concentrations and body weight were evaluated 48 h after diabetes induction and every 30 days thereafter. The nociceptive sensitivity was evaluated using the tail-flick apparatus. The animals were then transcardially perfused, and the spinal cords were post-fixed, cryoprotected and sectioned in a cryostat. Immunohistochemistry for calcitonin gene-related peptide analysis was performed on the dorsal horn of the spinal cord. RESULTS: The nociceptive sensitivity analysis revealed that, compared with the control and trained diabetic animals, the latency to tail deflection on the apparatus was longer for the diabetic animals. Optical densitometry demonstrated decreased calcitonin gene-related peptide immunoreactivity in the dorsal horn of the spinal cord in diabetic animals, which was reversed by treadmill training. CONCLUSION: We concluded that treadmill training can alleviate nociceptive hypoalgesia and reverse decreased calcitonin gene-related peptide immunoreactivity in the dorsal horn of the spinal cord of diabetic animals without pharmacological treatment. |
id |
UFRGS-2_18ebca418a233a59d9ff3abf62195507 |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/108687 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Nascimento, Patrícia Severo doLovatel, Gisele AgustiniIlha, JocemarXavier, Leder LealSchaan, Beatriz D'AgordAchaval-Elena, Matilde2014-12-25T02:10:27Z20121980-5322http://hdl.handle.net/10183/108687000877936OBJECTIVE: The aim of this study was to evaluate the effects of treadmill training on nociceptive sensitivity and immunoreactivity to calcitonin gene-related peptide in the dorsal horn of the spinal cord of diabetic rats. METHODS: Male Wistar rats were divided into three groups: control, diabetic and trained diabetic. Treadmill training was performed for 8 weeks. The blood glucose concentrations and body weight were evaluated 48 h after diabetes induction and every 30 days thereafter. The nociceptive sensitivity was evaluated using the tail-flick apparatus. The animals were then transcardially perfused, and the spinal cords were post-fixed, cryoprotected and sectioned in a cryostat. Immunohistochemistry for calcitonin gene-related peptide analysis was performed on the dorsal horn of the spinal cord. RESULTS: The nociceptive sensitivity analysis revealed that, compared with the control and trained diabetic animals, the latency to tail deflection on the apparatus was longer for the diabetic animals. Optical densitometry demonstrated decreased calcitonin gene-related peptide immunoreactivity in the dorsal horn of the spinal cord in diabetic animals, which was reversed by treadmill training. CONCLUSION: We concluded that treadmill training can alleviate nociceptive hypoalgesia and reverse decreased calcitonin gene-related peptide immunoreactivity in the dorsal horn of the spinal cord of diabetic animals without pharmacological treatment.application/pdfengClinics. São Paulo. Vol. 67, n. 9 (set. 2012), p. 1087-1091Neuropatias diabéticasPeptídeo relacionado com gene de calcitoninaCélulas do corno posteriorModelos animais de doençasHypoalgesiaTail-flick testCalcitonin gene-related peptide contentDorsal horn of spinal cordDiabetic neuropathyExercise alleviates hypoalgesia and increases the level of calcitonin gene-related peptide in the dorsal horn of the spinal cord of diabetic ratsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000877936.pdf000877936.pdfTexto completo (inglês)application/pdf223624http://www.lume.ufrgs.br/bitstream/10183/108687/1/000877936.pdf44d79a31e3cf93fe102e0ba8bc3de86eMD51TEXT000877936.pdf.txt000877936.pdf.txtExtracted Texttext/plain30256http://www.lume.ufrgs.br/bitstream/10183/108687/2/000877936.pdf.txt1aa3b815b8fad998e13826ecbc39f5d9MD52THUMBNAIL000877936.pdf.jpg000877936.pdf.jpgGenerated Thumbnailimage/jpeg1882http://www.lume.ufrgs.br/bitstream/10183/108687/3/000877936.pdf.jpg81ff2e80a87c182175571d26e62d6e03MD5310183/1086872018-10-23 08:32:58.861oai:www.lume.ufrgs.br:10183/108687Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-23T11:32:58Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Exercise alleviates hypoalgesia and increases the level of calcitonin gene-related peptide in the dorsal horn of the spinal cord of diabetic rats |
title |
Exercise alleviates hypoalgesia and increases the level of calcitonin gene-related peptide in the dorsal horn of the spinal cord of diabetic rats |
spellingShingle |
Exercise alleviates hypoalgesia and increases the level of calcitonin gene-related peptide in the dorsal horn of the spinal cord of diabetic rats Nascimento, Patrícia Severo do Neuropatias diabéticas Peptídeo relacionado com gene de calcitonina Células do corno posterior Modelos animais de doenças Hypoalgesia Tail-flick test Calcitonin gene-related peptide content Dorsal horn of spinal cord Diabetic neuropathy |
title_short |
Exercise alleviates hypoalgesia and increases the level of calcitonin gene-related peptide in the dorsal horn of the spinal cord of diabetic rats |
title_full |
Exercise alleviates hypoalgesia and increases the level of calcitonin gene-related peptide in the dorsal horn of the spinal cord of diabetic rats |
title_fullStr |
Exercise alleviates hypoalgesia and increases the level of calcitonin gene-related peptide in the dorsal horn of the spinal cord of diabetic rats |
title_full_unstemmed |
Exercise alleviates hypoalgesia and increases the level of calcitonin gene-related peptide in the dorsal horn of the spinal cord of diabetic rats |
title_sort |
Exercise alleviates hypoalgesia and increases the level of calcitonin gene-related peptide in the dorsal horn of the spinal cord of diabetic rats |
author |
Nascimento, Patrícia Severo do |
author_facet |
Nascimento, Patrícia Severo do Lovatel, Gisele Agustini Ilha, Jocemar Xavier, Leder Leal Schaan, Beatriz D'Agord Achaval-Elena, Matilde |
author_role |
author |
author2 |
Lovatel, Gisele Agustini Ilha, Jocemar Xavier, Leder Leal Schaan, Beatriz D'Agord Achaval-Elena, Matilde |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Nascimento, Patrícia Severo do Lovatel, Gisele Agustini Ilha, Jocemar Xavier, Leder Leal Schaan, Beatriz D'Agord Achaval-Elena, Matilde |
dc.subject.por.fl_str_mv |
Neuropatias diabéticas Peptídeo relacionado com gene de calcitonina Células do corno posterior Modelos animais de doenças |
topic |
Neuropatias diabéticas Peptídeo relacionado com gene de calcitonina Células do corno posterior Modelos animais de doenças Hypoalgesia Tail-flick test Calcitonin gene-related peptide content Dorsal horn of spinal cord Diabetic neuropathy |
dc.subject.eng.fl_str_mv |
Hypoalgesia Tail-flick test Calcitonin gene-related peptide content Dorsal horn of spinal cord Diabetic neuropathy |
description |
OBJECTIVE: The aim of this study was to evaluate the effects of treadmill training on nociceptive sensitivity and immunoreactivity to calcitonin gene-related peptide in the dorsal horn of the spinal cord of diabetic rats. METHODS: Male Wistar rats were divided into three groups: control, diabetic and trained diabetic. Treadmill training was performed for 8 weeks. The blood glucose concentrations and body weight were evaluated 48 h after diabetes induction and every 30 days thereafter. The nociceptive sensitivity was evaluated using the tail-flick apparatus. The animals were then transcardially perfused, and the spinal cords were post-fixed, cryoprotected and sectioned in a cryostat. Immunohistochemistry for calcitonin gene-related peptide analysis was performed on the dorsal horn of the spinal cord. RESULTS: The nociceptive sensitivity analysis revealed that, compared with the control and trained diabetic animals, the latency to tail deflection on the apparatus was longer for the diabetic animals. Optical densitometry demonstrated decreased calcitonin gene-related peptide immunoreactivity in the dorsal horn of the spinal cord in diabetic animals, which was reversed by treadmill training. CONCLUSION: We concluded that treadmill training can alleviate nociceptive hypoalgesia and reverse decreased calcitonin gene-related peptide immunoreactivity in the dorsal horn of the spinal cord of diabetic animals without pharmacological treatment. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2014-12-25T02:10:27Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/108687 |
dc.identifier.issn.pt_BR.fl_str_mv |
1980-5322 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000877936 |
identifier_str_mv |
1980-5322 000877936 |
url |
http://hdl.handle.net/10183/108687 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Clinics. São Paulo. Vol. 67, n. 9 (set. 2012), p. 1087-1091 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/108687/1/000877936.pdf http://www.lume.ufrgs.br/bitstream/10183/108687/2/000877936.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/108687/3/000877936.pdf.jpg |
bitstream.checksum.fl_str_mv |
44d79a31e3cf93fe102e0ba8bc3de86e 1aa3b815b8fad998e13826ecbc39f5d9 81ff2e80a87c182175571d26e62d6e03 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
|
_version_ |
1801224861052305408 |