Potential of medicinal plants from the Brazilian semi-arid region (Caatinga) against Staphylococcus epidermidis planktonic and biofilm lifestyles
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/253631 |
Resumo: | Ethnopharmacological relevance: Medicinal plants from the Caatinga, a Brazilian xeric shrubland, are used in folk medicine to treat infections. These ethnopharmacological data can contribute to obtaining new antimicrobial/antibiofilm extracts and natural product prototypes for the development of new drugs. The aim of this study was to investigate the antibiofilm and antibacterial activities of 45 aqueous extracts from 24 Caatinga plant species. Materials and methods: The effect of aqueous extracts on planktonic cells and on biofilm formation by Staphylococcus epidermidis was studied by the OD600 absorbance and by the crystal violet assay, respectively. Scanning electron microscopy (SEM) was used to generate comparative images of extract-treated and untreated biofilms. Chromatographic analyses were performed to characterize the active extracts. Results: The in vitro screening, at 0.4 mg/mL and 4.0 mg/mL, showed 20 plants effective in preventing biofilm formation and 13 plants able to inhibit planktonic bacterial growth. SEM images demonstrated distinct profiles of bacterial adhesion, matrix production and cell morphology according to different treatments and surfaces. The phytochemical analysis of the selected active extracts indicates the polyphenols, coumarins, steroids and terpenes as possible active compounds. Conclusion: This study describes the first antibiofilm and antibacterial screening of Caatinga plants against S. epidermidis. The evaluation presented in this study confirms several ethnopharmacological reports and can be utilized to identify new antibiofilm and antibacterial products against S. epidermidis from traditional Brazilian medicine. |
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Trentin, Danielle da SilvaGiordani, Raquel BrandtZimmer, Karine RigonSilva, Alexandre Gomes daSilva, Márcia Vanusa daCorreia, Maria Tereza dos SantosBaumvol, Israel Jacob RabinMacedo, Alexandre José2023-01-12T04:58:44Z20110378-8741http://hdl.handle.net/10183/253631000906258Ethnopharmacological relevance: Medicinal plants from the Caatinga, a Brazilian xeric shrubland, are used in folk medicine to treat infections. These ethnopharmacological data can contribute to obtaining new antimicrobial/antibiofilm extracts and natural product prototypes for the development of new drugs. The aim of this study was to investigate the antibiofilm and antibacterial activities of 45 aqueous extracts from 24 Caatinga plant species. Materials and methods: The effect of aqueous extracts on planktonic cells and on biofilm formation by Staphylococcus epidermidis was studied by the OD600 absorbance and by the crystal violet assay, respectively. Scanning electron microscopy (SEM) was used to generate comparative images of extract-treated and untreated biofilms. Chromatographic analyses were performed to characterize the active extracts. Results: The in vitro screening, at 0.4 mg/mL and 4.0 mg/mL, showed 20 plants effective in preventing biofilm formation and 13 plants able to inhibit planktonic bacterial growth. SEM images demonstrated distinct profiles of bacterial adhesion, matrix production and cell morphology according to different treatments and surfaces. The phytochemical analysis of the selected active extracts indicates the polyphenols, coumarins, steroids and terpenes as possible active compounds. Conclusion: This study describes the first antibiofilm and antibacterial screening of Caatinga plants against S. epidermidis. The evaluation presented in this study confirms several ethnopharmacological reports and can be utilized to identify new antibiofilm and antibacterial products against S. epidermidis from traditional Brazilian medicine.application/pdfengJournal of ethnopharmacology. Vol. 137, no. 1 (Sep. 2011), p. 327-335Plantas medicinaisAgentes antibacterianosBiofilmesCromatografiaMicroscopia eletrônica de varreduraFísicaPotential of medicinal plants from the Brazilian semi-arid region (Caatinga) against Staphylococcus epidermidis planktonic and biofilm lifestylesEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000906258.pdf.txt000906258.pdf.txtExtracted Texttext/plain45956http://www.lume.ufrgs.br/bitstream/10183/253631/2/000906258.pdf.txt20fec6f962b2624697140db7750f0015MD52ORIGINAL000906258.pdfTexto completo (inglês)application/pdf904582http://www.lume.ufrgs.br/bitstream/10183/253631/1/000906258.pdf55969d7930c0f9e31287ee7771361575MD5110183/2536312023-01-13 06:04:25.101646oai:www.lume.ufrgs.br:10183/253631Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-01-13T08:04:25Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Potential of medicinal plants from the Brazilian semi-arid region (Caatinga) against Staphylococcus epidermidis planktonic and biofilm lifestyles |
title |
Potential of medicinal plants from the Brazilian semi-arid region (Caatinga) against Staphylococcus epidermidis planktonic and biofilm lifestyles |
spellingShingle |
Potential of medicinal plants from the Brazilian semi-arid region (Caatinga) against Staphylococcus epidermidis planktonic and biofilm lifestyles Trentin, Danielle da Silva Plantas medicinais Agentes antibacterianos Biofilmes Cromatografia Microscopia eletrônica de varredura Física |
title_short |
Potential of medicinal plants from the Brazilian semi-arid region (Caatinga) against Staphylococcus epidermidis planktonic and biofilm lifestyles |
title_full |
Potential of medicinal plants from the Brazilian semi-arid region (Caatinga) against Staphylococcus epidermidis planktonic and biofilm lifestyles |
title_fullStr |
Potential of medicinal plants from the Brazilian semi-arid region (Caatinga) against Staphylococcus epidermidis planktonic and biofilm lifestyles |
title_full_unstemmed |
Potential of medicinal plants from the Brazilian semi-arid region (Caatinga) against Staphylococcus epidermidis planktonic and biofilm lifestyles |
title_sort |
Potential of medicinal plants from the Brazilian semi-arid region (Caatinga) against Staphylococcus epidermidis planktonic and biofilm lifestyles |
author |
Trentin, Danielle da Silva |
author_facet |
Trentin, Danielle da Silva Giordani, Raquel Brandt Zimmer, Karine Rigon Silva, Alexandre Gomes da Silva, Márcia Vanusa da Correia, Maria Tereza dos Santos Baumvol, Israel Jacob Rabin Macedo, Alexandre José |
author_role |
author |
author2 |
Giordani, Raquel Brandt Zimmer, Karine Rigon Silva, Alexandre Gomes da Silva, Márcia Vanusa da Correia, Maria Tereza dos Santos Baumvol, Israel Jacob Rabin Macedo, Alexandre José |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Trentin, Danielle da Silva Giordani, Raquel Brandt Zimmer, Karine Rigon Silva, Alexandre Gomes da Silva, Márcia Vanusa da Correia, Maria Tereza dos Santos Baumvol, Israel Jacob Rabin Macedo, Alexandre José |
dc.subject.por.fl_str_mv |
Plantas medicinais Agentes antibacterianos Biofilmes Cromatografia Microscopia eletrônica de varredura Física |
topic |
Plantas medicinais Agentes antibacterianos Biofilmes Cromatografia Microscopia eletrônica de varredura Física |
description |
Ethnopharmacological relevance: Medicinal plants from the Caatinga, a Brazilian xeric shrubland, are used in folk medicine to treat infections. These ethnopharmacological data can contribute to obtaining new antimicrobial/antibiofilm extracts and natural product prototypes for the development of new drugs. The aim of this study was to investigate the antibiofilm and antibacterial activities of 45 aqueous extracts from 24 Caatinga plant species. Materials and methods: The effect of aqueous extracts on planktonic cells and on biofilm formation by Staphylococcus epidermidis was studied by the OD600 absorbance and by the crystal violet assay, respectively. Scanning electron microscopy (SEM) was used to generate comparative images of extract-treated and untreated biofilms. Chromatographic analyses were performed to characterize the active extracts. Results: The in vitro screening, at 0.4 mg/mL and 4.0 mg/mL, showed 20 plants effective in preventing biofilm formation and 13 plants able to inhibit planktonic bacterial growth. SEM images demonstrated distinct profiles of bacterial adhesion, matrix production and cell morphology according to different treatments and surfaces. The phytochemical analysis of the selected active extracts indicates the polyphenols, coumarins, steroids and terpenes as possible active compounds. Conclusion: This study describes the first antibiofilm and antibacterial screening of Caatinga plants against S. epidermidis. The evaluation presented in this study confirms several ethnopharmacological reports and can be utilized to identify new antibiofilm and antibacterial products against S. epidermidis from traditional Brazilian medicine. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2023-01-12T04:58:44Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
format |
article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/253631 |
dc.identifier.issn.pt_BR.fl_str_mv |
0378-8741 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000906258 |
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0378-8741 000906258 |
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http://hdl.handle.net/10183/253631 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Journal of ethnopharmacology. Vol. 137, no. 1 (Sep. 2011), p. 327-335 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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