The RNA interference revolution
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/21216 |
Resumo: | The discovery of double-stranded RNA-mediated gene silencing has rapidly led to its use as a method of choice for blocking a gene, and has turned it into one of the most discussed topics in cell biology. Although still in its infancy, the field of RNA interference has already produced a vast array of results, mainly in Caenorhabditis elegans, but recently also in mammalian systems. Micro-RNAs are short hairpins of RNA capable of blocking translation, which are transcribed from genomic DNA and are implicated in several aspects from development to cell signaling. The present review discusses the main methods used for gene silencing in cell culture and animal models, including the selection of target sequences, delivery methods and strategies for a successful silencing. Expected developments are briefly discussed, ranging from reverse genetics to therapeutics. Thus, the development of the new paradigm of RNA-mediated gene silencing has produced two important advances: knowledge of a basic cellular mechanism present in the majority of eukaryotic cells and access to a potent and specific new method for gene silencing. |
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Lenz, Guido2010-04-24T04:15:47Z20050100-879Xhttp://hdl.handle.net/10183/21216000640340The discovery of double-stranded RNA-mediated gene silencing has rapidly led to its use as a method of choice for blocking a gene, and has turned it into one of the most discussed topics in cell biology. Although still in its infancy, the field of RNA interference has already produced a vast array of results, mainly in Caenorhabditis elegans, but recently also in mammalian systems. Micro-RNAs are short hairpins of RNA capable of blocking translation, which are transcribed from genomic DNA and are implicated in several aspects from development to cell signaling. The present review discusses the main methods used for gene silencing in cell culture and animal models, including the selection of target sequences, delivery methods and strategies for a successful silencing. Expected developments are briefly discussed, ranging from reverse genetics to therapeutics. Thus, the development of the new paradigm of RNA-mediated gene silencing has produced two important advances: knowledge of a basic cellular mechanism present in the majority of eukaryotic cells and access to a potent and specific new method for gene silencing.application/pdfengBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto. Vol. 38, n. 12/ind (dez. 2005), p. 1749-1757RNARNAiSiRNAMiRNAGene silencingKnockdownThe RNA interference revolutioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000640340.pdf.txt000640340.pdf.txtExtracted Texttext/plain33591http://www.lume.ufrgs.br/bitstream/10183/21216/2/000640340.pdf.txtd4400a9e9f0175134ae4fd5d30a258a6MD52ORIGINAL000640340.pdf000640340.pdfTexto completo (inglês)application/pdf563497http://www.lume.ufrgs.br/bitstream/10183/21216/1/000640340.pdf177115ebb64e36ec37fc12a627667720MD51THUMBNAIL000640340.pdf.jpg000640340.pdf.jpgGenerated Thumbnailimage/jpeg1665http://www.lume.ufrgs.br/bitstream/10183/21216/3/000640340.pdf.jpgea1b54d55015e949fa3da79d63690731MD5310183/212162018-10-08 08:08:50.373oai:www.lume.ufrgs.br:10183/21216Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-08T11:08:50Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
The RNA interference revolution |
title |
The RNA interference revolution |
spellingShingle |
The RNA interference revolution Lenz, Guido RNA RNAi SiRNA MiRNA Gene silencing Knockdown |
title_short |
The RNA interference revolution |
title_full |
The RNA interference revolution |
title_fullStr |
The RNA interference revolution |
title_full_unstemmed |
The RNA interference revolution |
title_sort |
The RNA interference revolution |
author |
Lenz, Guido |
author_facet |
Lenz, Guido |
author_role |
author |
dc.contributor.author.fl_str_mv |
Lenz, Guido |
dc.subject.por.fl_str_mv |
RNA |
topic |
RNA RNAi SiRNA MiRNA Gene silencing Knockdown |
dc.subject.eng.fl_str_mv |
RNAi SiRNA MiRNA Gene silencing Knockdown |
description |
The discovery of double-stranded RNA-mediated gene silencing has rapidly led to its use as a method of choice for blocking a gene, and has turned it into one of the most discussed topics in cell biology. Although still in its infancy, the field of RNA interference has already produced a vast array of results, mainly in Caenorhabditis elegans, but recently also in mammalian systems. Micro-RNAs are short hairpins of RNA capable of blocking translation, which are transcribed from genomic DNA and are implicated in several aspects from development to cell signaling. The present review discusses the main methods used for gene silencing in cell culture and animal models, including the selection of target sequences, delivery methods and strategies for a successful silencing. Expected developments are briefly discussed, ranging from reverse genetics to therapeutics. Thus, the development of the new paradigm of RNA-mediated gene silencing has produced two important advances: knowledge of a basic cellular mechanism present in the majority of eukaryotic cells and access to a potent and specific new method for gene silencing. |
publishDate |
2005 |
dc.date.issued.fl_str_mv |
2005 |
dc.date.accessioned.fl_str_mv |
2010-04-24T04:15:47Z |
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info:eu-repo/semantics/article info:eu-repo/semantics/other |
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article |
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http://hdl.handle.net/10183/21216 |
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0100-879X |
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000640340 |
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http://hdl.handle.net/10183/21216 |
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eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto. Vol. 38, n. 12/ind (dez. 2005), p. 1749-1757 |
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openAccess |
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