Gadolinium increases the vascular reactivity of rat aortic rings
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/37320 |
Resumo: | Gadolinium (Gd) blocks intra- and extracellular ATP hydrolysis. We determined whether Gd affects vascular reactivity to contractile responses to phenylephrine (PHE) by blocking aortic ectonucleoside triphosphate diphosphohydrolase (E-NTPDase). Wistar rats of both sexes (260-300 g, 23 females, 7 males) were used. Experiments were performed before and after incubation of aortic rings with 3 μM Gd. Concentration-response curves to PHE (0.1 nM to 0.1 mM) were obtained in the presence and absence of endothelium, after incubation with 100 μM L-NAME, 10 μM losartan, or 10 μM enalaprilat. Gd significantly increased the maximum response (control: 72.3 ± 3.5; Gd: 101.3 ± 6.4%) and sensitivity (control: 6.6 ± 0.1; Gd: 10.5 ± 2.8%) to PHE. To investigate the blockade of E-NTPDase activity by Gd, we added 1 mM ATP to the bath. ATP reduced smooth muscle tension and Gd increased its relaxing effect (control: -33.5 ± 4.1; Gd: -47.4 ± 4.1%). Endothelial damage abolished the effect of Gd on the contractile responses to PHE (control: 132.6 ± 8.6; Gd: 122.4 ± 7.1%). L-NAME + Gd in the presence of endothelium reduced PHE contractile responses (control/L-NAME: 151.1 ± 28.8; L-NAME + Gd: 67.9 ± 19% AUC). ATP hydrolysis was reduced after Gd administration, which led to ATP accumulation in the nutrient solution and reduced ADP concentration, while adenosine levels remained the same. Incubation with Gd plus losartan and enalaprilat eliminated the pressor effects of Gd. Gd increased vascular reactivity to PHE regardless of the reduction of E-NTPDase activity and adenosine production. Moreover, the increased reactivity to PHE promoted by Gd was endothelium-dependent, reducing NO bioavailability and involving an increased stimulation of angiotensin-converting enzyme and angiotensin II AT1 receptors. |
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Angeli, Jully KelyRamos, Denise BarbosaCasali, Emerson AndreSouza, Diogo Onofre Gomes deSarkis, João José FreitasStefanon, IvanitaVassallo, Dalton ValentimFürstenau, Cristina Ribas2012-03-03T01:24:55Z20110100-879Xhttp://hdl.handle.net/10183/37320000818611Gadolinium (Gd) blocks intra- and extracellular ATP hydrolysis. We determined whether Gd affects vascular reactivity to contractile responses to phenylephrine (PHE) by blocking aortic ectonucleoside triphosphate diphosphohydrolase (E-NTPDase). Wistar rats of both sexes (260-300 g, 23 females, 7 males) were used. Experiments were performed before and after incubation of aortic rings with 3 μM Gd. Concentration-response curves to PHE (0.1 nM to 0.1 mM) were obtained in the presence and absence of endothelium, after incubation with 100 μM L-NAME, 10 μM losartan, or 10 μM enalaprilat. Gd significantly increased the maximum response (control: 72.3 ± 3.5; Gd: 101.3 ± 6.4%) and sensitivity (control: 6.6 ± 0.1; Gd: 10.5 ± 2.8%) to PHE. To investigate the blockade of E-NTPDase activity by Gd, we added 1 mM ATP to the bath. ATP reduced smooth muscle tension and Gd increased its relaxing effect (control: -33.5 ± 4.1; Gd: -47.4 ± 4.1%). Endothelial damage abolished the effect of Gd on the contractile responses to PHE (control: 132.6 ± 8.6; Gd: 122.4 ± 7.1%). L-NAME + Gd in the presence of endothelium reduced PHE contractile responses (control/L-NAME: 151.1 ± 28.8; L-NAME + Gd: 67.9 ± 19% AUC). ATP hydrolysis was reduced after Gd administration, which led to ATP accumulation in the nutrient solution and reduced ADP concentration, while adenosine levels remained the same. Incubation with Gd plus losartan and enalaprilat eliminated the pressor effects of Gd. Gd increased vascular reactivity to PHE regardless of the reduction of E-NTPDase activity and adenosine production. Moreover, the increased reactivity to PHE promoted by Gd was endothelium-dependent, reducing NO bioavailability and involving an increased stimulation of angiotensin-converting enzyme and angiotensin II AT1 receptors.application/pdfengBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 44, no. 5 (May 2011), p. 445-452HipertensãoGadolínioAdenosinaAngiotensina IIGadoliniumE-NTPDaseAdenosineAngiotensin IIAT1 receptorGadolinium increases the vascular reactivity of rat aortic ringsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000818611.pdf.txt000818611.pdf.txtExtracted Texttext/plain34464http://www.lume.ufrgs.br/bitstream/10183/37320/2/000818611.pdf.txt315b4eeb950c5b82e375d580152286c3MD52ORIGINAL000818611.pdf000818611.pdfTexto completo (inglês)application/pdf502895http://www.lume.ufrgs.br/bitstream/10183/37320/1/000818611.pdf48f0978f093984598d78bfc033b170b8MD51THUMBNAIL000818611.pdf.jpg000818611.pdf.jpgGenerated Thumbnailimage/jpeg2147http://www.lume.ufrgs.br/bitstream/10183/37320/3/000818611.pdf.jpgcc115a73a3ecbd0b1e0fb4ff8c2368bfMD5310183/373202021-11-20 06:15:17.570337oai:www.lume.ufrgs.br:10183/37320Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-11-20T08:15:17Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Gadolinium increases the vascular reactivity of rat aortic rings |
title |
Gadolinium increases the vascular reactivity of rat aortic rings |
spellingShingle |
Gadolinium increases the vascular reactivity of rat aortic rings Angeli, Jully Kely Hipertensão Gadolínio Adenosina Angiotensina II Gadolinium E-NTPDase Adenosine Angiotensin II AT1 receptor |
title_short |
Gadolinium increases the vascular reactivity of rat aortic rings |
title_full |
Gadolinium increases the vascular reactivity of rat aortic rings |
title_fullStr |
Gadolinium increases the vascular reactivity of rat aortic rings |
title_full_unstemmed |
Gadolinium increases the vascular reactivity of rat aortic rings |
title_sort |
Gadolinium increases the vascular reactivity of rat aortic rings |
author |
Angeli, Jully Kely |
author_facet |
Angeli, Jully Kely Ramos, Denise Barbosa Casali, Emerson Andre Souza, Diogo Onofre Gomes de Sarkis, João José Freitas Stefanon, Ivanita Vassallo, Dalton Valentim Fürstenau, Cristina Ribas |
author_role |
author |
author2 |
Ramos, Denise Barbosa Casali, Emerson Andre Souza, Diogo Onofre Gomes de Sarkis, João José Freitas Stefanon, Ivanita Vassallo, Dalton Valentim Fürstenau, Cristina Ribas |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Angeli, Jully Kely Ramos, Denise Barbosa Casali, Emerson Andre Souza, Diogo Onofre Gomes de Sarkis, João José Freitas Stefanon, Ivanita Vassallo, Dalton Valentim Fürstenau, Cristina Ribas |
dc.subject.por.fl_str_mv |
Hipertensão Gadolínio Adenosina Angiotensina II |
topic |
Hipertensão Gadolínio Adenosina Angiotensina II Gadolinium E-NTPDase Adenosine Angiotensin II AT1 receptor |
dc.subject.eng.fl_str_mv |
Gadolinium E-NTPDase Adenosine Angiotensin II AT1 receptor |
description |
Gadolinium (Gd) blocks intra- and extracellular ATP hydrolysis. We determined whether Gd affects vascular reactivity to contractile responses to phenylephrine (PHE) by blocking aortic ectonucleoside triphosphate diphosphohydrolase (E-NTPDase). Wistar rats of both sexes (260-300 g, 23 females, 7 males) were used. Experiments were performed before and after incubation of aortic rings with 3 μM Gd. Concentration-response curves to PHE (0.1 nM to 0.1 mM) were obtained in the presence and absence of endothelium, after incubation with 100 μM L-NAME, 10 μM losartan, or 10 μM enalaprilat. Gd significantly increased the maximum response (control: 72.3 ± 3.5; Gd: 101.3 ± 6.4%) and sensitivity (control: 6.6 ± 0.1; Gd: 10.5 ± 2.8%) to PHE. To investigate the blockade of E-NTPDase activity by Gd, we added 1 mM ATP to the bath. ATP reduced smooth muscle tension and Gd increased its relaxing effect (control: -33.5 ± 4.1; Gd: -47.4 ± 4.1%). Endothelial damage abolished the effect of Gd on the contractile responses to PHE (control: 132.6 ± 8.6; Gd: 122.4 ± 7.1%). L-NAME + Gd in the presence of endothelium reduced PHE contractile responses (control/L-NAME: 151.1 ± 28.8; L-NAME + Gd: 67.9 ± 19% AUC). ATP hydrolysis was reduced after Gd administration, which led to ATP accumulation in the nutrient solution and reduced ADP concentration, while adenosine levels remained the same. Incubation with Gd plus losartan and enalaprilat eliminated the pressor effects of Gd. Gd increased vascular reactivity to PHE regardless of the reduction of E-NTPDase activity and adenosine production. Moreover, the increased reactivity to PHE promoted by Gd was endothelium-dependent, reducing NO bioavailability and involving an increased stimulation of angiotensin-converting enzyme and angiotensin II AT1 receptors. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2012-03-03T01:24:55Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
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0100-879X |
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000818611 |
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http://hdl.handle.net/10183/37320 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 44, no. 5 (May 2011), p. 445-452 |
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