Poor unstable midgut microbiome of hard ticks contrasts with abundant and stable monospecific microbiome in ovaries
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/212098 |
Resumo: | Culture-independent metagenomic methodologies have enabled detection and identification of microorganisms in various biological systems and often revealed complex and unknown microbiomes. In many organisms, the microbiome outnumbers the host cells and greatly affects the host biology and fitness. Ticks are hematophagous ectoparasites with a wide host range. They vector a number of human and animal pathogens and also directly cause major economic losses in livestock. Although several reports on a tick midgut microbiota show a diverse bacterial community, in most cases the size of the bacterial population has not been determined. In this study, the microbiome was quantified in the midgut and ovaries of the ticks Ixodes ricinus and Rhipicephalus microplus before, during, and after blood feeding. Although the size of bacterial community in the midgut fluctuated with blood feeding, it was overall extremely low in comparison to that of other hematophagous arthropods. In addition, the tick ovarian microbiome of both tick species exceeded the midgut 16S rDNA copy numbers by several orders of magnitude. This indicates that the ratio of a tick midgut/ovary microbiome represents an exception to the general biology of other metazoans. In addition to the very low abundance, the tick midgut diversity in I. ricinus was variable and that is in contrast to that found in the tick ovary. The ovary of I. ricinus had a very low bacterial diversity and a very high and stable bacterial abundance with the dominant endosymbiont, Midichloria sp. The elucidation of this aspect of tick biology highlights a unique tissue-specific microbial-invertebrate host interaction. |
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Guizzo, Melina GarciaNeupane, SaraswotiKucera, MatejPerner, JanFrantová, HelenaVaz Junior, Itabajara da SilvaOliveira, Pedro Lagerblad deKopacek, PetrZurek, Ludek2020-07-18T03:48:29Z20202235-2988http://hdl.handle.net/10183/212098001114775Culture-independent metagenomic methodologies have enabled detection and identification of microorganisms in various biological systems and often revealed complex and unknown microbiomes. In many organisms, the microbiome outnumbers the host cells and greatly affects the host biology and fitness. Ticks are hematophagous ectoparasites with a wide host range. They vector a number of human and animal pathogens and also directly cause major economic losses in livestock. Although several reports on a tick midgut microbiota show a diverse bacterial community, in most cases the size of the bacterial population has not been determined. In this study, the microbiome was quantified in the midgut and ovaries of the ticks Ixodes ricinus and Rhipicephalus microplus before, during, and after blood feeding. Although the size of bacterial community in the midgut fluctuated with blood feeding, it was overall extremely low in comparison to that of other hematophagous arthropods. In addition, the tick ovarian microbiome of both tick species exceeded the midgut 16S rDNA copy numbers by several orders of magnitude. This indicates that the ratio of a tick midgut/ovary microbiome represents an exception to the general biology of other metazoans. In addition to the very low abundance, the tick midgut diversity in I. ricinus was variable and that is in contrast to that found in the tick ovary. The ovary of I. ricinus had a very low bacterial diversity and a very high and stable bacterial abundance with the dominant endosymbiont, Midichloria sp. The elucidation of this aspect of tick biology highlights a unique tissue-specific microbial-invertebrate host interaction.application/pdfengFrontiers in Cellular and Infection Microbiology. Lausanne. Vol. 10 (May 2020), 211, 10 p.Ixodes ricinusRhipicephalus microplusMicrobiotaIntestinoOvárioCoxiellaMidichloria mitochondriiAnálise de sequênciaTickIxodes ricinusRhipicephalus microplusMidgut microbiomeOvary microbiomeSymbiosisPoor unstable midgut microbiome of hard ticks contrasts with abundant and stable monospecific microbiome in ovariesEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001114775.pdf.txt001114775.pdf.txtExtracted Texttext/plain52649http://www.lume.ufrgs.br/bitstream/10183/212098/2/001114775.pdf.txt2e4b180b0b0cb91da40a8ea81bea59d3MD52ORIGINAL001114775.pdfTexto completo (inglês)application/pdf1270433http://www.lume.ufrgs.br/bitstream/10183/212098/1/001114775.pdf4389b91d78f58fe4531a2cd6cc091fbfMD5110183/2120982020-07-19 03:36:49.945298oai:www.lume.ufrgs.br:10183/212098Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2020-07-19T06:36:49Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Poor unstable midgut microbiome of hard ticks contrasts with abundant and stable monospecific microbiome in ovaries |
title |
Poor unstable midgut microbiome of hard ticks contrasts with abundant and stable monospecific microbiome in ovaries |
spellingShingle |
Poor unstable midgut microbiome of hard ticks contrasts with abundant and stable monospecific microbiome in ovaries Guizzo, Melina Garcia Ixodes ricinus Rhipicephalus microplus Microbiota Intestino Ovário Coxiella Midichloria mitochondrii Análise de sequência Tick Ixodes ricinus Rhipicephalus microplus Midgut microbiome Ovary microbiome Symbiosis |
title_short |
Poor unstable midgut microbiome of hard ticks contrasts with abundant and stable monospecific microbiome in ovaries |
title_full |
Poor unstable midgut microbiome of hard ticks contrasts with abundant and stable monospecific microbiome in ovaries |
title_fullStr |
Poor unstable midgut microbiome of hard ticks contrasts with abundant and stable monospecific microbiome in ovaries |
title_full_unstemmed |
Poor unstable midgut microbiome of hard ticks contrasts with abundant and stable monospecific microbiome in ovaries |
title_sort |
Poor unstable midgut microbiome of hard ticks contrasts with abundant and stable monospecific microbiome in ovaries |
author |
Guizzo, Melina Garcia |
author_facet |
Guizzo, Melina Garcia Neupane, Saraswoti Kucera, Matej Perner, Jan Frantová, Helena Vaz Junior, Itabajara da Silva Oliveira, Pedro Lagerblad de Kopacek, Petr Zurek, Ludek |
author_role |
author |
author2 |
Neupane, Saraswoti Kucera, Matej Perner, Jan Frantová, Helena Vaz Junior, Itabajara da Silva Oliveira, Pedro Lagerblad de Kopacek, Petr Zurek, Ludek |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Guizzo, Melina Garcia Neupane, Saraswoti Kucera, Matej Perner, Jan Frantová, Helena Vaz Junior, Itabajara da Silva Oliveira, Pedro Lagerblad de Kopacek, Petr Zurek, Ludek |
dc.subject.por.fl_str_mv |
Ixodes ricinus Rhipicephalus microplus Microbiota Intestino Ovário Coxiella Midichloria mitochondrii Análise de sequência |
topic |
Ixodes ricinus Rhipicephalus microplus Microbiota Intestino Ovário Coxiella Midichloria mitochondrii Análise de sequência Tick Ixodes ricinus Rhipicephalus microplus Midgut microbiome Ovary microbiome Symbiosis |
dc.subject.eng.fl_str_mv |
Tick Ixodes ricinus Rhipicephalus microplus Midgut microbiome Ovary microbiome Symbiosis |
description |
Culture-independent metagenomic methodologies have enabled detection and identification of microorganisms in various biological systems and often revealed complex and unknown microbiomes. In many organisms, the microbiome outnumbers the host cells and greatly affects the host biology and fitness. Ticks are hematophagous ectoparasites with a wide host range. They vector a number of human and animal pathogens and also directly cause major economic losses in livestock. Although several reports on a tick midgut microbiota show a diverse bacterial community, in most cases the size of the bacterial population has not been determined. In this study, the microbiome was quantified in the midgut and ovaries of the ticks Ixodes ricinus and Rhipicephalus microplus before, during, and after blood feeding. Although the size of bacterial community in the midgut fluctuated with blood feeding, it was overall extremely low in comparison to that of other hematophagous arthropods. In addition, the tick ovarian microbiome of both tick species exceeded the midgut 16S rDNA copy numbers by several orders of magnitude. This indicates that the ratio of a tick midgut/ovary microbiome represents an exception to the general biology of other metazoans. In addition to the very low abundance, the tick midgut diversity in I. ricinus was variable and that is in contrast to that found in the tick ovary. The ovary of I. ricinus had a very low bacterial diversity and a very high and stable bacterial abundance with the dominant endosymbiont, Midichloria sp. The elucidation of this aspect of tick biology highlights a unique tissue-specific microbial-invertebrate host interaction. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-07-18T03:48:29Z |
dc.date.issued.fl_str_mv |
2020 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/212098 |
dc.identifier.issn.pt_BR.fl_str_mv |
2235-2988 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001114775 |
identifier_str_mv |
2235-2988 001114775 |
url |
http://hdl.handle.net/10183/212098 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Frontiers in Cellular and Infection Microbiology. Lausanne. Vol. 10 (May 2020), 211, 10 p. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
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