Aversive stimulation during the stress-hyporesponsive period does not affect the number of corticotroph cells in neonatal male rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 1997 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/21204 |
Resumo: | Immunohistochemistry was used to evaluate the effects of neonatal handling and aversive stimulation during the first 10 days of life on the number of corticotrophs in the anterior lobe of the pituitary of 11-dayold male Wistar rats. Since adult rats handled during infancy respond with reduced corticosterone secretion in response to stressors and with less behavior inhibition in novel environments, we assumed that neonatal stimulation could affect pituitary morphology during this critical period of cell differentiation. Three groups of animals were studied: intact (no manipulation, N = 5), handled (N = 5) and stimulated (submitted to 3 different aversive stimuli, N = 5). The percentage of ACTH-immunoreactive cells in the anterior lobe of the pituitary (number of ACTH-stained cells divided by total number of cells) was determined by examining three slices per pituitary in which a minimum of 200 cells were counted by two independent researchers. Although animals during the neonatal period are less reactive to stresslike stimulation in terms of ACTH and corticosterone secretion, results showed that the relative number of ACTH-stained cells of neonatal handled (0.25 ± 0.01) and aversive stimulated (0.29 ± 0.03) rats was not significantly different from intact (0.30 ± 0.03) animals. Neonatal stimulation may have a differential effect on the various subpopulations of corticotroph cells in the anterior pituitary. |
id |
UFRGS-2_7c58f67e32a689ea3b85cda154c94959 |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/21204 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Lucion, Aldo BoltenAguiar, Claudia Enk deStefani, Luciana Paula CadorePadoin, Maristela JorgeCoutinho, Ligia Maria Barbosa2010-04-24T04:15:42Z19970100-879Xhttp://hdl.handle.net/10183/21204000576697Immunohistochemistry was used to evaluate the effects of neonatal handling and aversive stimulation during the first 10 days of life on the number of corticotrophs in the anterior lobe of the pituitary of 11-dayold male Wistar rats. Since adult rats handled during infancy respond with reduced corticosterone secretion in response to stressors and with less behavior inhibition in novel environments, we assumed that neonatal stimulation could affect pituitary morphology during this critical period of cell differentiation. Three groups of animals were studied: intact (no manipulation, N = 5), handled (N = 5) and stimulated (submitted to 3 different aversive stimuli, N = 5). The percentage of ACTH-immunoreactive cells in the anterior lobe of the pituitary (number of ACTH-stained cells divided by total number of cells) was determined by examining three slices per pituitary in which a minimum of 200 cells were counted by two independent researchers. Although animals during the neonatal period are less reactive to stresslike stimulation in terms of ACTH and corticosterone secretion, results showed that the relative number of ACTH-stained cells of neonatal handled (0.25 ± 0.01) and aversive stimulated (0.29 ± 0.03) rats was not significantly different from intact (0.30 ± 0.03) animals. Neonatal stimulation may have a differential effect on the various subpopulations of corticotroph cells in the anterior pituitary.application/pdfengBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 30, n. 12 (1997), p. 1463-1466FisiologiaACTHImmunohistochemistryPituitaryNeonatal handlingStressAversive stimulation during the stress-hyporesponsive period does not affect the number of corticotroph cells in neonatal male ratsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000576697.pdf000576697.pdfTexto completo (inglês)application/pdf144600http://www.lume.ufrgs.br/bitstream/10183/21204/1/000576697.pdfd8980b8b56e4cc87c31e3051f3f8e37fMD51TEXT000576697.pdf.txt000576697.pdf.txtExtracted Texttext/plain14314http://www.lume.ufrgs.br/bitstream/10183/21204/2/000576697.pdf.txt51cbbd8955168c29cdf585c74ae388a7MD52THUMBNAIL000576697.pdf.jpg000576697.pdf.jpgGenerated Thumbnailimage/jpeg1748http://www.lume.ufrgs.br/bitstream/10183/21204/3/000576697.pdf.jpg9c70db91e2c7789893060c5b9f105344MD5310183/212042021-11-20 05:45:37.16663oai:www.lume.ufrgs.br:10183/21204Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-11-20T07:45:37Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Aversive stimulation during the stress-hyporesponsive period does not affect the number of corticotroph cells in neonatal male rats |
title |
Aversive stimulation during the stress-hyporesponsive period does not affect the number of corticotroph cells in neonatal male rats |
spellingShingle |
Aversive stimulation during the stress-hyporesponsive period does not affect the number of corticotroph cells in neonatal male rats Lucion, Aldo Bolten Fisiologia ACTH Immunohistochemistry Pituitary Neonatal handling Stress |
title_short |
Aversive stimulation during the stress-hyporesponsive period does not affect the number of corticotroph cells in neonatal male rats |
title_full |
Aversive stimulation during the stress-hyporesponsive period does not affect the number of corticotroph cells in neonatal male rats |
title_fullStr |
Aversive stimulation during the stress-hyporesponsive period does not affect the number of corticotroph cells in neonatal male rats |
title_full_unstemmed |
Aversive stimulation during the stress-hyporesponsive period does not affect the number of corticotroph cells in neonatal male rats |
title_sort |
Aversive stimulation during the stress-hyporesponsive period does not affect the number of corticotroph cells in neonatal male rats |
author |
Lucion, Aldo Bolten |
author_facet |
Lucion, Aldo Bolten Aguiar, Claudia Enk de Stefani, Luciana Paula Cadore Padoin, Maristela Jorge Coutinho, Ligia Maria Barbosa |
author_role |
author |
author2 |
Aguiar, Claudia Enk de Stefani, Luciana Paula Cadore Padoin, Maristela Jorge Coutinho, Ligia Maria Barbosa |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Lucion, Aldo Bolten Aguiar, Claudia Enk de Stefani, Luciana Paula Cadore Padoin, Maristela Jorge Coutinho, Ligia Maria Barbosa |
dc.subject.por.fl_str_mv |
Fisiologia |
topic |
Fisiologia ACTH Immunohistochemistry Pituitary Neonatal handling Stress |
dc.subject.eng.fl_str_mv |
ACTH Immunohistochemistry Pituitary Neonatal handling Stress |
description |
Immunohistochemistry was used to evaluate the effects of neonatal handling and aversive stimulation during the first 10 days of life on the number of corticotrophs in the anterior lobe of the pituitary of 11-dayold male Wistar rats. Since adult rats handled during infancy respond with reduced corticosterone secretion in response to stressors and with less behavior inhibition in novel environments, we assumed that neonatal stimulation could affect pituitary morphology during this critical period of cell differentiation. Three groups of animals were studied: intact (no manipulation, N = 5), handled (N = 5) and stimulated (submitted to 3 different aversive stimuli, N = 5). The percentage of ACTH-immunoreactive cells in the anterior lobe of the pituitary (number of ACTH-stained cells divided by total number of cells) was determined by examining three slices per pituitary in which a minimum of 200 cells were counted by two independent researchers. Although animals during the neonatal period are less reactive to stresslike stimulation in terms of ACTH and corticosterone secretion, results showed that the relative number of ACTH-stained cells of neonatal handled (0.25 ± 0.01) and aversive stimulated (0.29 ± 0.03) rats was not significantly different from intact (0.30 ± 0.03) animals. Neonatal stimulation may have a differential effect on the various subpopulations of corticotroph cells in the anterior pituitary. |
publishDate |
1997 |
dc.date.issued.fl_str_mv |
1997 |
dc.date.accessioned.fl_str_mv |
2010-04-24T04:15:42Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/21204 |
dc.identifier.issn.pt_BR.fl_str_mv |
0100-879X |
dc.identifier.nrb.pt_BR.fl_str_mv |
000576697 |
identifier_str_mv |
0100-879X 000576697 |
url |
http://hdl.handle.net/10183/21204 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 30, n. 12 (1997), p. 1463-1466 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/21204/1/000576697.pdf http://www.lume.ufrgs.br/bitstream/10183/21204/2/000576697.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/21204/3/000576697.pdf.jpg |
bitstream.checksum.fl_str_mv |
d8980b8b56e4cc87c31e3051f3f8e37f 51cbbd8955168c29cdf585c74ae388a7 9c70db91e2c7789893060c5b9f105344 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
|
_version_ |
1801224711058751488 |