Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisae diploid strain
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/23381 |
Resumo: | The Escherichia coli RecA protein (RecAp) has been demonstrated to induce mutagenesis in yeast cells, although there is still little information on the role of the RecAp in yeast recombination events. We evaluated spontaneous and induced general recombination in vegetative and meiotic cells of the XS2316 strain of the yeast Saccharomyces cerevisiae bearing the recA gene. We found that RecAp decreased reciprocal recombination, gene conversion and intrachromosomal recombination and promoted an increase in error-prone processes in both vegetative and meiotic cells, while its negative effect on meiotic recombination blocked ascospore formation. |
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Morais Junior, Marcos Antonio deSantos, Jose Ferreira dosHenriques, João Antonio Pêgas2010-06-05T04:17:23Z20031415-4757http://hdl.handle.net/10183/23381000476059The Escherichia coli RecA protein (RecAp) has been demonstrated to induce mutagenesis in yeast cells, although there is still little information on the role of the RecAp in yeast recombination events. We evaluated spontaneous and induced general recombination in vegetative and meiotic cells of the XS2316 strain of the yeast Saccharomyces cerevisiae bearing the recA gene. We found that RecAp decreased reciprocal recombination, gene conversion and intrachromosomal recombination and promoted an increase in error-prone processes in both vegetative and meiotic cells, while its negative effect on meiotic recombination blocked ascospore formation.application/pdfengGenetics and molecular biology. Ribeirão Preto. Vol. 26, no. 2 (June 2003), p.213-220GenéticaSaccharomyces cerevisiaeDNADNA repairGenetic recombinationRecA geneSporulationYeastExpression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisae diploid straininfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000476059.pdf000476059.pdfTexto completo (inglês)application/pdf228261http://www.lume.ufrgs.br/bitstream/10183/23381/1/000476059.pdf8b023175a697fc3dbea90b560a19c904MD51TEXT000476059.pdf.txt000476059.pdf.txtExtracted Texttext/plain29074http://www.lume.ufrgs.br/bitstream/10183/23381/2/000476059.pdf.txteb8f01757214fdc5b2475b0a56e61a24MD52THUMBNAIL000476059.pdf.jpg000476059.pdf.jpgGenerated Thumbnailimage/jpeg1821http://www.lume.ufrgs.br/bitstream/10183/23381/3/000476059.pdf.jpga88ec6fc86c18041ef97f92ea4a69c4cMD5310183/233812019-06-20 02:36:22.229281oai:www.lume.ufrgs.br:10183/23381Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-06-20T05:36:22Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisae diploid strain |
title |
Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisae diploid strain |
spellingShingle |
Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisae diploid strain Morais Junior, Marcos Antonio de Genética Saccharomyces cerevisiae DNA DNA repair Genetic recombination RecA gene Sporulation Yeast |
title_short |
Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisae diploid strain |
title_full |
Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisae diploid strain |
title_fullStr |
Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisae diploid strain |
title_full_unstemmed |
Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisae diploid strain |
title_sort |
Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisae diploid strain |
author |
Morais Junior, Marcos Antonio de |
author_facet |
Morais Junior, Marcos Antonio de Santos, Jose Ferreira dos Henriques, João Antonio Pêgas |
author_role |
author |
author2 |
Santos, Jose Ferreira dos Henriques, João Antonio Pêgas |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Morais Junior, Marcos Antonio de Santos, Jose Ferreira dos Henriques, João Antonio Pêgas |
dc.subject.por.fl_str_mv |
Genética Saccharomyces cerevisiae DNA |
topic |
Genética Saccharomyces cerevisiae DNA DNA repair Genetic recombination RecA gene Sporulation Yeast |
dc.subject.eng.fl_str_mv |
DNA repair Genetic recombination RecA gene Sporulation Yeast |
description |
The Escherichia coli RecA protein (RecAp) has been demonstrated to induce mutagenesis in yeast cells, although there is still little information on the role of the RecAp in yeast recombination events. We evaluated spontaneous and induced general recombination in vegetative and meiotic cells of the XS2316 strain of the yeast Saccharomyces cerevisiae bearing the recA gene. We found that RecAp decreased reciprocal recombination, gene conversion and intrachromosomal recombination and promoted an increase in error-prone processes in both vegetative and meiotic cells, while its negative effect on meiotic recombination blocked ascospore formation. |
publishDate |
2003 |
dc.date.issued.fl_str_mv |
2003 |
dc.date.accessioned.fl_str_mv |
2010-06-05T04:17:23Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/23381 |
dc.identifier.issn.pt_BR.fl_str_mv |
1415-4757 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000476059 |
identifier_str_mv |
1415-4757 000476059 |
url |
http://hdl.handle.net/10183/23381 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Genetics and molecular biology. Ribeirão Preto. Vol. 26, no. 2 (June 2003), p.213-220 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
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Repositório Institucional da UFRGS |
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