Human embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) growth on nanostructured Ti6Al4V surfaces
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/187922 |
Resumo: | Nanotexturing processes that focus on enhancing the bone-implant contact, such as electropolishing, have been proposed. The aim of this work was to evaluate the influence of Ti6Al4V surface morphology on human embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) growth. Three surface treatments were used in this study: mechanically polished samples and two types of electropolished samples that were treated for 4 min and 12 min, respectively. The systems were characterized by atomic force microscopy, contact profilometry, X-ray diffraction, and wettability. Each system was submitted to a cell culture containing hESCs-MP cells for 14 days, and the resultant cell growth on each system was then evaluated. The results indicated that surfaces with higher nanometric and micrometric roughnesses experienced greater hESCs-MP cell growth in osteogenic media. The same behavior was not observed for cell growth in non-osteogenic media due to the absence of dexamethasone, which is responsible for controlling protein adsorption on the surface. |
id |
UFRGS-2_a62f185ee0966bb0b50014254d8faf4a |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/187922 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Antonini, Leonardo MarascaSantos Júnior, Adilar Gonçalves dosReilly, GwendolenMalfatti, Célia de Fraga2019-01-19T02:33:23Z20181516-1439http://hdl.handle.net/10183/187922001082572Nanotexturing processes that focus on enhancing the bone-implant contact, such as electropolishing, have been proposed. The aim of this work was to evaluate the influence of Ti6Al4V surface morphology on human embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) growth. Three surface treatments were used in this study: mechanically polished samples and two types of electropolished samples that were treated for 4 min and 12 min, respectively. The systems were characterized by atomic force microscopy, contact profilometry, X-ray diffraction, and wettability. Each system was submitted to a cell culture containing hESCs-MP cells for 14 days, and the resultant cell growth on each system was then evaluated. The results indicated that surfaces with higher nanometric and micrometric roughnesses experienced greater hESCs-MP cell growth in osteogenic media. The same behavior was not observed for cell growth in non-osteogenic media due to the absence of dexamethasone, which is responsible for controlling protein adsorption on the surface.application/pdfengMaterials research : ibero-american journal of materials. São Carlos. Vol. 21, no. 5 (July 2018), e20171081, [10] p.Células-tronco embrionáriasEletropolimentoLigas de titânioMolhabilidadehESCs-MP cellsTi6Al4VElectropolishingNanometric and micrometric roughnessWettabilityHuman embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) growth on nanostructured Ti6Al4V surfacesinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001082572.pdf.txt001082572.pdf.txtExtracted Texttext/plain48359http://www.lume.ufrgs.br/bitstream/10183/187922/2/001082572.pdf.txtbdc87e6b9be7580292d580456aa25715MD52ORIGINAL001082572.pdfTexto completo (inglês)application/pdf1824048http://www.lume.ufrgs.br/bitstream/10183/187922/1/001082572.pdfa27d3a96fd9271d38ddcff61c28d8bd8MD5110183/1879222019-01-20 02:31:56.07342oai:www.lume.ufrgs.br:10183/187922Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-01-20T04:31:56Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Human embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) growth on nanostructured Ti6Al4V surfaces |
title |
Human embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) growth on nanostructured Ti6Al4V surfaces |
spellingShingle |
Human embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) growth on nanostructured Ti6Al4V surfaces Antonini, Leonardo Marasca Células-tronco embrionárias Eletropolimento Ligas de titânio Molhabilidade hESCs-MP cells Ti6Al4V Electropolishing Nanometric and micrometric roughness Wettability |
title_short |
Human embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) growth on nanostructured Ti6Al4V surfaces |
title_full |
Human embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) growth on nanostructured Ti6Al4V surfaces |
title_fullStr |
Human embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) growth on nanostructured Ti6Al4V surfaces |
title_full_unstemmed |
Human embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) growth on nanostructured Ti6Al4V surfaces |
title_sort |
Human embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) growth on nanostructured Ti6Al4V surfaces |
author |
Antonini, Leonardo Marasca |
author_facet |
Antonini, Leonardo Marasca Santos Júnior, Adilar Gonçalves dos Reilly, Gwendolen Malfatti, Célia de Fraga |
author_role |
author |
author2 |
Santos Júnior, Adilar Gonçalves dos Reilly, Gwendolen Malfatti, Célia de Fraga |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Antonini, Leonardo Marasca Santos Júnior, Adilar Gonçalves dos Reilly, Gwendolen Malfatti, Célia de Fraga |
dc.subject.por.fl_str_mv |
Células-tronco embrionárias Eletropolimento Ligas de titânio Molhabilidade |
topic |
Células-tronco embrionárias Eletropolimento Ligas de titânio Molhabilidade hESCs-MP cells Ti6Al4V Electropolishing Nanometric and micrometric roughness Wettability |
dc.subject.eng.fl_str_mv |
hESCs-MP cells Ti6Al4V Electropolishing Nanometric and micrometric roughness Wettability |
description |
Nanotexturing processes that focus on enhancing the bone-implant contact, such as electropolishing, have been proposed. The aim of this work was to evaluate the influence of Ti6Al4V surface morphology on human embryonic stem cell-derived mesenchymal progenitor (hESCs-MP) growth. Three surface treatments were used in this study: mechanically polished samples and two types of electropolished samples that were treated for 4 min and 12 min, respectively. The systems were characterized by atomic force microscopy, contact profilometry, X-ray diffraction, and wettability. Each system was submitted to a cell culture containing hESCs-MP cells for 14 days, and the resultant cell growth on each system was then evaluated. The results indicated that surfaces with higher nanometric and micrometric roughnesses experienced greater hESCs-MP cell growth in osteogenic media. The same behavior was not observed for cell growth in non-osteogenic media due to the absence of dexamethasone, which is responsible for controlling protein adsorption on the surface. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2019-01-19T02:33:23Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/187922 |
dc.identifier.issn.pt_BR.fl_str_mv |
1516-1439 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001082572 |
identifier_str_mv |
1516-1439 001082572 |
url |
http://hdl.handle.net/10183/187922 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Materials research : ibero-american journal of materials. São Carlos. Vol. 21, no. 5 (July 2018), e20171081, [10] p. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/187922/2/001082572.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/187922/1/001082572.pdf |
bitstream.checksum.fl_str_mv |
bdc87e6b9be7580292d580456aa25715 a27d3a96fd9271d38ddcff61c28d8bd8 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
|
_version_ |
1801224960703725568 |