Androgen receptor isoforms expression in benign prostatic hyperplasia and primary prostate cancer
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/182694 |
Resumo: | The role of molecular changes in the androgen receptor (AR) as AR variants (AR-Vs) is not clear in the pathophysiology of benign prostatic hyperplasia (BPH) and hormone-naïve PCa. The aim of the current work was to identify the presence of AR isoforms in benign tissue and primary PCa, and to evaluate the possible association with tumor aggressiveness and biochemical recurrence in primary PCa. The mRNA levels of full length AR (AR-FL) and AR-Vs (AR-V1, AR-V4 and AR-V7) were measured using RT-qPCR. The protein expression of AR-FL (AR-CTD and AR-NTD) and AR-V7 were evaluated by the H-Score in immunohistochemistry (IHC). All investigated mRNA targets were expressed both in BPH and PCa. AR-FL mRNA levels were similar in both groups. AR-V4 mRNA expression showed higher levels in BPH, and AR-V1 and AR-V7 mRNA expression were higher in PCa. The AR-V7 protein showed a similar H-Score in both groups, while AR-CTD and AR-NTD were higher in nuclei of epithelial cells from BPH. These results support the assumption that these constitutively active isoforms of AR are involved in the pathophysiology of primary PCa and BPH. The role of AR-Vs and their possible modulation by steroid tissue levels in distinct types of prostate tumors needs to be elucidated to help guide the best clinical management of these diseases. |
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Hillebrand, Ana CarolinePizzolato, Lolita SchneiderSilva Neto, BrasilBranchini, GiseleBrum, Ilma Simoni2018-09-26T02:33:48Z20181932-6203http://hdl.handle.net/10183/182694001074860The role of molecular changes in the androgen receptor (AR) as AR variants (AR-Vs) is not clear in the pathophysiology of benign prostatic hyperplasia (BPH) and hormone-naïve PCa. The aim of the current work was to identify the presence of AR isoforms in benign tissue and primary PCa, and to evaluate the possible association with tumor aggressiveness and biochemical recurrence in primary PCa. The mRNA levels of full length AR (AR-FL) and AR-Vs (AR-V1, AR-V4 and AR-V7) were measured using RT-qPCR. The protein expression of AR-FL (AR-CTD and AR-NTD) and AR-V7 were evaluated by the H-Score in immunohistochemistry (IHC). All investigated mRNA targets were expressed both in BPH and PCa. AR-FL mRNA levels were similar in both groups. AR-V4 mRNA expression showed higher levels in BPH, and AR-V1 and AR-V7 mRNA expression were higher in PCa. The AR-V7 protein showed a similar H-Score in both groups, while AR-CTD and AR-NTD were higher in nuclei of epithelial cells from BPH. These results support the assumption that these constitutively active isoforms of AR are involved in the pathophysiology of primary PCa and BPH. The role of AR-Vs and their possible modulation by steroid tissue levels in distinct types of prostate tumors needs to be elucidated to help guide the best clinical management of these diseases.application/pdfengPLoS ONE. San Francisco. Vol. 13, no. 7 (Jul. 2018), e0200613, 17 p.Receptores androgênicosIsoformas de proteínasExpressão gênicaNeoplasias da próstataHiperplasia prostáticaAndrogen receptor isoforms expression in benign prostatic hyperplasia and primary prostate cancerEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001074860.pdfTexto completo (inglês)application/pdf6982583http://www.lume.ufrgs.br/bitstream/10183/182694/1/001074860.pdf76cb3f86a7521e82dd8e46c974ff31dbMD51TEXT001074860.pdf.txt001074860.pdf.txtExtracted Texttext/plain52785http://www.lume.ufrgs.br/bitstream/10183/182694/2/001074860.pdf.txtd0782a9c6e768681f2712a040a3c6e10MD52THUMBNAIL001074860.pdf.jpg001074860.pdf.jpgGenerated Thumbnailimage/jpeg2032http://www.lume.ufrgs.br/bitstream/10183/182694/3/001074860.pdf.jpg9a650b1636643d07e0a350b82fa38eb0MD5310183/1826942024-05-23 06:42:43.539015oai:www.lume.ufrgs.br:10183/182694Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-05-23T09:42:43Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Androgen receptor isoforms expression in benign prostatic hyperplasia and primary prostate cancer |
title |
Androgen receptor isoforms expression in benign prostatic hyperplasia and primary prostate cancer |
spellingShingle |
Androgen receptor isoforms expression in benign prostatic hyperplasia and primary prostate cancer Hillebrand, Ana Caroline Receptores androgênicos Isoformas de proteínas Expressão gênica Neoplasias da próstata Hiperplasia prostática |
title_short |
Androgen receptor isoforms expression in benign prostatic hyperplasia and primary prostate cancer |
title_full |
Androgen receptor isoforms expression in benign prostatic hyperplasia and primary prostate cancer |
title_fullStr |
Androgen receptor isoforms expression in benign prostatic hyperplasia and primary prostate cancer |
title_full_unstemmed |
Androgen receptor isoforms expression in benign prostatic hyperplasia and primary prostate cancer |
title_sort |
Androgen receptor isoforms expression in benign prostatic hyperplasia and primary prostate cancer |
author |
Hillebrand, Ana Caroline |
author_facet |
Hillebrand, Ana Caroline Pizzolato, Lolita Schneider Silva Neto, Brasil Branchini, Gisele Brum, Ilma Simoni |
author_role |
author |
author2 |
Pizzolato, Lolita Schneider Silva Neto, Brasil Branchini, Gisele Brum, Ilma Simoni |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Hillebrand, Ana Caroline Pizzolato, Lolita Schneider Silva Neto, Brasil Branchini, Gisele Brum, Ilma Simoni |
dc.subject.por.fl_str_mv |
Receptores androgênicos Isoformas de proteínas Expressão gênica Neoplasias da próstata Hiperplasia prostática |
topic |
Receptores androgênicos Isoformas de proteínas Expressão gênica Neoplasias da próstata Hiperplasia prostática |
description |
The role of molecular changes in the androgen receptor (AR) as AR variants (AR-Vs) is not clear in the pathophysiology of benign prostatic hyperplasia (BPH) and hormone-naïve PCa. The aim of the current work was to identify the presence of AR isoforms in benign tissue and primary PCa, and to evaluate the possible association with tumor aggressiveness and biochemical recurrence in primary PCa. The mRNA levels of full length AR (AR-FL) and AR-Vs (AR-V1, AR-V4 and AR-V7) were measured using RT-qPCR. The protein expression of AR-FL (AR-CTD and AR-NTD) and AR-V7 were evaluated by the H-Score in immunohistochemistry (IHC). All investigated mRNA targets were expressed both in BPH and PCa. AR-FL mRNA levels were similar in both groups. AR-V4 mRNA expression showed higher levels in BPH, and AR-V1 and AR-V7 mRNA expression were higher in PCa. The AR-V7 protein showed a similar H-Score in both groups, while AR-CTD and AR-NTD were higher in nuclei of epithelial cells from BPH. These results support the assumption that these constitutively active isoforms of AR are involved in the pathophysiology of primary PCa and BPH. The role of AR-Vs and their possible modulation by steroid tissue levels in distinct types of prostate tumors needs to be elucidated to help guide the best clinical management of these diseases. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-09-26T02:33:48Z |
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2018 |
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eng |
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PLoS ONE. San Francisco. Vol. 13, no. 7 (Jul. 2018), e0200613, 17 p. |
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openAccess |
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