Dietary sugars modulate bacterial-fungal interactions in saliva and inter-kingdom biofilm formation on apatitic surface
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/272207 |
Resumo: | Bacteria and fungi can interact to form inter-kingdom biofilms in the oral cavity. Streptococcus mutans and Candida albicans are frequently detected in saliva and in dental biofilms associated with early childhood caries (tooth-decay), a prevalent oral disease induced by dietary sugars. However, how different sugars influence this bacterial-fungal interaction remains unclear. Here, we investigate whether specific sugars affect the inter-kingdom interaction in saliva and subsequent biofilm formation on tooth-mimetic surfaces. The microbes were incubated in saliva containing common dietary sugars (glucose and fructose, sucrose, starch, and combinations) and analyzed via fluorescence imaging and quantitative computational analyses. The bacterial and fungal cells in saliva were then transferred to hydroxyapatite discs (tooth mimic) to allow microbial binding and biofilm development. We found diverse bacterial-fungal aggregates which varied in size, structure, and spatial organization depending on the type of sugars. Sucrose and starch+sucrose induced the formation of large mixed-species aggregates characterized by bacterial clusters co-bound with fungal cells, whereas mostly single-cells were found in the absence of sugar or in the presence of glucose and fructose. Notably, both colonization and further growth on the apatitic surface were dependent on sugar-mediated aggregation, leading to biofilms with distinctive spatial organizations and 3D architectures Starch+sucrose and sucrose-mediated aggregates developed into large and highly acidogenic biofilms with complex network of bacterial and fungal cells (yeast and hyphae) surrounded by an intricate matrix of extracellular glucans. In contrast, biofilms originated from glucose and fructose-mediated consortia (or without sugar) were sparsely distributed on the surface without structural integration, growing predominantly as individual species with reduced acidogenicity. These findings reveal the impact of dietary sugars on inter-kingdom interactions in saliva and how they mediate biofilm formation with distinctive structural organization and varying acidogenicity implicated with human tooth-decay |
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Negrini, Thais de CássiaRen, ZhiMiao, YilanKim, DongyeopSimón-Soro, ÁureaLiu, YuanKoo, HyunArthur, Rodrigo Alex2024-02-27T04:57:59Z20222235-2988http://hdl.handle.net/10183/272207001196091Bacteria and fungi can interact to form inter-kingdom biofilms in the oral cavity. Streptococcus mutans and Candida albicans are frequently detected in saliva and in dental biofilms associated with early childhood caries (tooth-decay), a prevalent oral disease induced by dietary sugars. However, how different sugars influence this bacterial-fungal interaction remains unclear. Here, we investigate whether specific sugars affect the inter-kingdom interaction in saliva and subsequent biofilm formation on tooth-mimetic surfaces. The microbes were incubated in saliva containing common dietary sugars (glucose and fructose, sucrose, starch, and combinations) and analyzed via fluorescence imaging and quantitative computational analyses. The bacterial and fungal cells in saliva were then transferred to hydroxyapatite discs (tooth mimic) to allow microbial binding and biofilm development. We found diverse bacterial-fungal aggregates which varied in size, structure, and spatial organization depending on the type of sugars. Sucrose and starch+sucrose induced the formation of large mixed-species aggregates characterized by bacterial clusters co-bound with fungal cells, whereas mostly single-cells were found in the absence of sugar or in the presence of glucose and fructose. Notably, both colonization and further growth on the apatitic surface were dependent on sugar-mediated aggregation, leading to biofilms with distinctive spatial organizations and 3D architectures Starch+sucrose and sucrose-mediated aggregates developed into large and highly acidogenic biofilms with complex network of bacterial and fungal cells (yeast and hyphae) surrounded by an intricate matrix of extracellular glucans. In contrast, biofilms originated from glucose and fructose-mediated consortia (or without sugar) were sparsely distributed on the surface without structural integration, growing predominantly as individual species with reduced acidogenicity. These findings reveal the impact of dietary sugars on inter-kingdom interactions in saliva and how they mediate biofilm formation with distinctive structural organization and varying acidogenicity implicated with human tooth-decayapplication/pdfengFrontiers in cellular and infection microbiology. Lausanne. Vol. 12 (2022), 993640, 13 p.Streptococcus mutansCandida albicansSalivaSacaroseMatriz extracelular de substâncias poliméricasS. mutansC. albicansSalivaSucroseInter-kingdom aggregateEPSDietary sugars modulate bacterial-fungal interactions in saliva and inter-kingdom biofilm formation on apatitic surfaceEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001196091.pdf.txt001196091.pdf.txtExtracted Texttext/plain60940http://www.lume.ufrgs.br/bitstream/10183/272207/2/001196091.pdf.txt6ddae7cb21c8cacc5d54cfcfecee4d4aMD52ORIGINAL001196091.pdfTexto completo (inglês)application/pdf2829630http://www.lume.ufrgs.br/bitstream/10183/272207/1/001196091.pdfece875a998e561af88e7c29822938cb9MD5110183/2722072024-02-28 05:03:01.467163oai:www.lume.ufrgs.br:10183/272207Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-02-28T08:03:01Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Dietary sugars modulate bacterial-fungal interactions in saliva and inter-kingdom biofilm formation on apatitic surface |
title |
Dietary sugars modulate bacterial-fungal interactions in saliva and inter-kingdom biofilm formation on apatitic surface |
spellingShingle |
Dietary sugars modulate bacterial-fungal interactions in saliva and inter-kingdom biofilm formation on apatitic surface Negrini, Thais de Cássia Streptococcus mutans Candida albicans Saliva Sacarose Matriz extracelular de substâncias poliméricas S. mutans C. albicans Saliva Sucrose Inter-kingdom aggregate EPS |
title_short |
Dietary sugars modulate bacterial-fungal interactions in saliva and inter-kingdom biofilm formation on apatitic surface |
title_full |
Dietary sugars modulate bacterial-fungal interactions in saliva and inter-kingdom biofilm formation on apatitic surface |
title_fullStr |
Dietary sugars modulate bacterial-fungal interactions in saliva and inter-kingdom biofilm formation on apatitic surface |
title_full_unstemmed |
Dietary sugars modulate bacterial-fungal interactions in saliva and inter-kingdom biofilm formation on apatitic surface |
title_sort |
Dietary sugars modulate bacterial-fungal interactions in saliva and inter-kingdom biofilm formation on apatitic surface |
author |
Negrini, Thais de Cássia |
author_facet |
Negrini, Thais de Cássia Ren, Zhi Miao, Yilan Kim, Dongyeop Simón-Soro, Áurea Liu, Yuan Koo, Hyun Arthur, Rodrigo Alex |
author_role |
author |
author2 |
Ren, Zhi Miao, Yilan Kim, Dongyeop Simón-Soro, Áurea Liu, Yuan Koo, Hyun Arthur, Rodrigo Alex |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Negrini, Thais de Cássia Ren, Zhi Miao, Yilan Kim, Dongyeop Simón-Soro, Áurea Liu, Yuan Koo, Hyun Arthur, Rodrigo Alex |
dc.subject.por.fl_str_mv |
Streptococcus mutans Candida albicans Saliva Sacarose Matriz extracelular de substâncias poliméricas |
topic |
Streptococcus mutans Candida albicans Saliva Sacarose Matriz extracelular de substâncias poliméricas S. mutans C. albicans Saliva Sucrose Inter-kingdom aggregate EPS |
dc.subject.eng.fl_str_mv |
S. mutans C. albicans Saliva Sucrose Inter-kingdom aggregate EPS |
description |
Bacteria and fungi can interact to form inter-kingdom biofilms in the oral cavity. Streptococcus mutans and Candida albicans are frequently detected in saliva and in dental biofilms associated with early childhood caries (tooth-decay), a prevalent oral disease induced by dietary sugars. However, how different sugars influence this bacterial-fungal interaction remains unclear. Here, we investigate whether specific sugars affect the inter-kingdom interaction in saliva and subsequent biofilm formation on tooth-mimetic surfaces. The microbes were incubated in saliva containing common dietary sugars (glucose and fructose, sucrose, starch, and combinations) and analyzed via fluorescence imaging and quantitative computational analyses. The bacterial and fungal cells in saliva were then transferred to hydroxyapatite discs (tooth mimic) to allow microbial binding and biofilm development. We found diverse bacterial-fungal aggregates which varied in size, structure, and spatial organization depending on the type of sugars. Sucrose and starch+sucrose induced the formation of large mixed-species aggregates characterized by bacterial clusters co-bound with fungal cells, whereas mostly single-cells were found in the absence of sugar or in the presence of glucose and fructose. Notably, both colonization and further growth on the apatitic surface were dependent on sugar-mediated aggregation, leading to biofilms with distinctive spatial organizations and 3D architectures Starch+sucrose and sucrose-mediated aggregates developed into large and highly acidogenic biofilms with complex network of bacterial and fungal cells (yeast and hyphae) surrounded by an intricate matrix of extracellular glucans. In contrast, biofilms originated from glucose and fructose-mediated consortia (or without sugar) were sparsely distributed on the surface without structural integration, growing predominantly as individual species with reduced acidogenicity. These findings reveal the impact of dietary sugars on inter-kingdom interactions in saliva and how they mediate biofilm formation with distinctive structural organization and varying acidogenicity implicated with human tooth-decay |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022 |
dc.date.accessioned.fl_str_mv |
2024-02-27T04:57:59Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/272207 |
dc.identifier.issn.pt_BR.fl_str_mv |
2235-2988 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001196091 |
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2235-2988 001196091 |
url |
http://hdl.handle.net/10183/272207 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Frontiers in cellular and infection microbiology. Lausanne. Vol. 12 (2022), 993640, 13 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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