Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MS
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/225844 |
Resumo: | The use of oral fluid as a biological matrix to monitor the use of drugs of abuse is a global trend because it presents several advantages and good correlation to the blood level.Thus, the present work aimed to develop and validate an analytical method for quantification and detection of solvents used as inhalants of abuse in oral fluid (OF), using Quantisal as collector device by headspace and gas chromatography coupled with a mass detector (HS-GC/MS). Chromatographic separation was performed with a ZB-BAC1 column and the total time of analysis was 11.8 min. The method showed good linearity (correlation coefficient higher than 0.99 for all solvents). The limits of detection ranged from 0.05 to 5 mg/L, while the lower limits of quantification ranged from 2.5 to 12.5 mg/L. Accuracy, precision, matrix effect, and residual effect presented satisfactory results, meeting the criteria accepted for the validation of bioanalytical methods. The method showed good selectivity considering that, for solvents coeluting at the same retention time, resolution was performed by the mass detector. The method developed proved to be adequate when applied in OF samples from users of drugs and may be used to monitor the abuse of inhalants in routine forensic analyses. |
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Coppe, Bruna CláudiaBorille, Bruna TassiFiorentin, Taís ReginaJacques, Ana Laura BemvenutiFagundes, Ana CláudiaCastro, Stela Maris de JezusRemy, Lysa SilveiraPechansky, FlavioLimberger, Renata Pereira2021-08-18T04:41:50Z20161687-8760http://hdl.handle.net/10183/225844000992686The use of oral fluid as a biological matrix to monitor the use of drugs of abuse is a global trend because it presents several advantages and good correlation to the blood level.Thus, the present work aimed to develop and validate an analytical method for quantification and detection of solvents used as inhalants of abuse in oral fluid (OF), using Quantisal as collector device by headspace and gas chromatography coupled with a mass detector (HS-GC/MS). Chromatographic separation was performed with a ZB-BAC1 column and the total time of analysis was 11.8 min. The method showed good linearity (correlation coefficient higher than 0.99 for all solvents). The limits of detection ranged from 0.05 to 5 mg/L, while the lower limits of quantification ranged from 2.5 to 12.5 mg/L. Accuracy, precision, matrix effect, and residual effect presented satisfactory results, meeting the criteria accepted for the validation of bioanalytical methods. The method showed good selectivity considering that, for solvents coeluting at the same retention time, resolution was performed by the mass detector. The method developed proved to be adequate when applied in OF samples from users of drugs and may be used to monitor the abuse of inhalants in routine forensic analyses.application/pdfengInternational Journal of Analytical Chemistry. New York. Vol. 2016 (April 2016), 9 f. Article ID 1029286Estatística aplicadaMultianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MSEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000992686.pdf.txt000992686.pdf.txtExtracted Texttext/plain38586http://www.lume.ufrgs.br/bitstream/10183/225844/2/000992686.pdf.txt4ea8e62533119cf6cbf7ad105aa05397MD52ORIGINAL000992686.pdfTexto completo (inglês)application/pdf1648686http://www.lume.ufrgs.br/bitstream/10183/225844/1/000992686.pdf6d1d2f7af199baddcbf57b01acd78ab7MD5110183/2258442021-08-18 05:20:55.897oai:www.lume.ufrgs.br:10183/225844Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-08-18T08:20:55Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MS |
title |
Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MS |
spellingShingle |
Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MS Coppe, Bruna Cláudia Estatística aplicada |
title_short |
Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MS |
title_full |
Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MS |
title_fullStr |
Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MS |
title_full_unstemmed |
Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MS |
title_sort |
Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MS |
author |
Coppe, Bruna Cláudia |
author_facet |
Coppe, Bruna Cláudia Borille, Bruna Tassi Fiorentin, Taís Regina Jacques, Ana Laura Bemvenuti Fagundes, Ana Cláudia Castro, Stela Maris de Jezus Remy, Lysa Silveira Pechansky, Flavio Limberger, Renata Pereira |
author_role |
author |
author2 |
Borille, Bruna Tassi Fiorentin, Taís Regina Jacques, Ana Laura Bemvenuti Fagundes, Ana Cláudia Castro, Stela Maris de Jezus Remy, Lysa Silveira Pechansky, Flavio Limberger, Renata Pereira |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Coppe, Bruna Cláudia Borille, Bruna Tassi Fiorentin, Taís Regina Jacques, Ana Laura Bemvenuti Fagundes, Ana Cláudia Castro, Stela Maris de Jezus Remy, Lysa Silveira Pechansky, Flavio Limberger, Renata Pereira |
dc.subject.por.fl_str_mv |
Estatística aplicada |
topic |
Estatística aplicada |
description |
The use of oral fluid as a biological matrix to monitor the use of drugs of abuse is a global trend because it presents several advantages and good correlation to the blood level.Thus, the present work aimed to develop and validate an analytical method for quantification and detection of solvents used as inhalants of abuse in oral fluid (OF), using Quantisal as collector device by headspace and gas chromatography coupled with a mass detector (HS-GC/MS). Chromatographic separation was performed with a ZB-BAC1 column and the total time of analysis was 11.8 min. The method showed good linearity (correlation coefficient higher than 0.99 for all solvents). The limits of detection ranged from 0.05 to 5 mg/L, while the lower limits of quantification ranged from 2.5 to 12.5 mg/L. Accuracy, precision, matrix effect, and residual effect presented satisfactory results, meeting the criteria accepted for the validation of bioanalytical methods. The method showed good selectivity considering that, for solvents coeluting at the same retention time, resolution was performed by the mass detector. The method developed proved to be adequate when applied in OF samples from users of drugs and may be used to monitor the abuse of inhalants in routine forensic analyses. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2021-08-18T04:41:50Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/225844 |
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1687-8760 |
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000992686 |
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http://hdl.handle.net/10183/225844 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
International Journal of Analytical Chemistry. New York. Vol. 2016 (April 2016), 9 f. Article ID 1029286 |
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