A3 receptor agonist modulates IL-1β hippocampus levels in a rat model of neuropathic pain

• Autor(a) principal: Cioato, Stefania Giotti
• Data de Publicação: 2022
• Outros Autores: Medeiros, Liciane Fernandes, Lopes, Bettega Costa, Medeiros, Helouise Richardt, Caumo, Wolnei, Roesler, Rafael, Torres, Iraci Lucena da Silva
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/250315
• topic: Receptor A3 de adenosina; Citocinas; Dimetil sulfóxido; Neuralgia; Fatores de crescimento neural; Ratos; Adenosine A3 receptor; Cytokine; DMSO; N6-(3-iodobenzyl) adenosine-5’-methyluronamide (IB-MECA); Neuropathic pain; Neurotrophin; Rats

Introduction: Considering the lack of specific treatments for neuropathic pain, this study aimed to evaluate the effect of a single dose of adenosine A3 receptor IB-MECA on inflammatory and neurotrophic parameters in rats subjected to a neuropathic pain model. Methods: 64 adult male Wistar rats were used. Neuropathic pain was induced by chronic constriction injury (CCI) of the sciatic nerve and the treatment consisted of a 0.5 μmol/kg dose of IB-MECA, a selective A3 adenosine receptor agonist, dissolved in 3% DMSO; vehicle groups received DMSO 3% in saline solution, and morphine groups received 5 mg/kg. Cerebral cortex and hippocampus IL-1β, BDNF, and NGF levels were determined by Enzyme-Linked Immunosorbent assay. Results: The main outcome was that a single dose of IB-MECA was able to modulate the IL-1β hippocampal levels in neuropathic pain induced by CCI and the DMSO increased IL-1β and NGF hippocampal levels in sham-operated rats. However, we did not observe this effect when the DMSO was used as vehicle for IB-MECA, indicating that IB-MECA was able to prevent the effect of DMSO. Conclusions: Considering that the IL-1β role in neuropathic pain and the contributions of the hippocampus are well explored, our result corroborates the relationship between the A3 receptor and the process of chronic pain maintenance.