Morphplogical characterization and genetic analysis of Drechslera teres isolates
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/23348 |
Resumo: | Net blotch, caused by the phytopathogen Drechslera teres, is a common disease of barley (Hordeum vulgare L) and is responsible for large economic losses in some barley growing areas. In this study the morphology and genetic variability of eight D. teres isolates from different regions of the Brazilian state of Rio Grande do Sul were investigated. Colony morphology was studied on potato-dextrose-agar (PDA) and genetic variability investigated using the random amplified polymorphic-DNA (RAPD) technique. 27 commercially available primers were tested of which 16 were selected for use in polymorphic analysis due to their good resolution and reproducibility. Similarity coefficients were used to construct dendrograms based on colony morphology and RAPD data showing the relationship between the eight isolates studied. Colony morphology showed variability between the isolates while RAPD assays showed high similarity coefficients, but grouping of the isolates according to the geographic origins of the seeds from which they were isolated was not possible. |
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Frazzon, Ana Paula GuedesMatsumura, Aida Terezinha SantosVan der Sand, Sueli Terezinha2010-06-05T04:17:17Z20021415-4757http://hdl.handle.net/10183/23348000373793Net blotch, caused by the phytopathogen Drechslera teres, is a common disease of barley (Hordeum vulgare L) and is responsible for large economic losses in some barley growing areas. In this study the morphology and genetic variability of eight D. teres isolates from different regions of the Brazilian state of Rio Grande do Sul were investigated. Colony morphology was studied on potato-dextrose-agar (PDA) and genetic variability investigated using the random amplified polymorphic-DNA (RAPD) technique. 27 commercially available primers were tested of which 16 were selected for use in polymorphic analysis due to their good resolution and reproducibility. Similarity coefficients were used to construct dendrograms based on colony morphology and RAPD data showing the relationship between the eight isolates studied. Colony morphology showed variability between the isolates while RAPD assays showed high similarity coefficients, but grouping of the isolates according to the geographic origins of the seeds from which they were isolated was not possible.application/pdfengGenetics and molecular biology. Ribeirão Preto, SP. Vol. 25, no. 2 (June 2002), p. 235-241PolimorfismoDrechslera teresDrechslera teresBarleyNet blotchDNA polymorphismRAPDMorphplogical characterization and genetic analysis of Drechslera teres isolatesinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000373793.pdf000373793.pdfTexto completo (inglês)application/pdf811923http://www.lume.ufrgs.br/bitstream/10183/23348/1/000373793.pdfd397b7cfd032f7a170204c46e33f9832MD51TEXT000373793.pdf.txt000373793.pdf.txtExtracted Texttext/plain22462http://www.lume.ufrgs.br/bitstream/10183/23348/2/000373793.pdf.txt7784d3c03a4dca82f38f6e4fd6fcd488MD52THUMBNAIL000373793.pdf.jpg000373793.pdf.jpgGenerated Thumbnailimage/jpeg1814http://www.lume.ufrgs.br/bitstream/10183/23348/3/000373793.pdf.jpg78455b2ac9f9d03c2db28029ce35e9f8MD5310183/233482023-09-16 03:39:39.911249oai:www.lume.ufrgs.br:10183/23348Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-09-16T06:39:39Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Morphplogical characterization and genetic analysis of Drechslera teres isolates |
title |
Morphplogical characterization and genetic analysis of Drechslera teres isolates |
spellingShingle |
Morphplogical characterization and genetic analysis of Drechslera teres isolates Frazzon, Ana Paula Guedes Polimorfismo Drechslera teres Drechslera teres Barley Net blotch DNA polymorphism RAPD |
title_short |
Morphplogical characterization and genetic analysis of Drechslera teres isolates |
title_full |
Morphplogical characterization and genetic analysis of Drechslera teres isolates |
title_fullStr |
Morphplogical characterization and genetic analysis of Drechslera teres isolates |
title_full_unstemmed |
Morphplogical characterization and genetic analysis of Drechslera teres isolates |
title_sort |
Morphplogical characterization and genetic analysis of Drechslera teres isolates |
author |
Frazzon, Ana Paula Guedes |
author_facet |
Frazzon, Ana Paula Guedes Matsumura, Aida Terezinha Santos Van der Sand, Sueli Terezinha |
author_role |
author |
author2 |
Matsumura, Aida Terezinha Santos Van der Sand, Sueli Terezinha |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Frazzon, Ana Paula Guedes Matsumura, Aida Terezinha Santos Van der Sand, Sueli Terezinha |
dc.subject.por.fl_str_mv |
Polimorfismo Drechslera teres |
topic |
Polimorfismo Drechslera teres Drechslera teres Barley Net blotch DNA polymorphism RAPD |
dc.subject.eng.fl_str_mv |
Drechslera teres Barley Net blotch DNA polymorphism RAPD |
description |
Net blotch, caused by the phytopathogen Drechslera teres, is a common disease of barley (Hordeum vulgare L) and is responsible for large economic losses in some barley growing areas. In this study the morphology and genetic variability of eight D. teres isolates from different regions of the Brazilian state of Rio Grande do Sul were investigated. Colony morphology was studied on potato-dextrose-agar (PDA) and genetic variability investigated using the random amplified polymorphic-DNA (RAPD) technique. 27 commercially available primers were tested of which 16 were selected for use in polymorphic analysis due to their good resolution and reproducibility. Similarity coefficients were used to construct dendrograms based on colony morphology and RAPD data showing the relationship between the eight isolates studied. Colony morphology showed variability between the isolates while RAPD assays showed high similarity coefficients, but grouping of the isolates according to the geographic origins of the seeds from which they were isolated was not possible. |
publishDate |
2002 |
dc.date.issued.fl_str_mv |
2002 |
dc.date.accessioned.fl_str_mv |
2010-06-05T04:17:17Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/23348 |
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1415-4757 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000373793 |
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1415-4757 000373793 |
url |
http://hdl.handle.net/10183/23348 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Genetics and molecular biology. Ribeirão Preto, SP. Vol. 25, no. 2 (June 2002), p. 235-241 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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