Modeling and simulation of breakthrough curves during purification of two chitosanases from Metarhizium anisopliae using ion-exchange with expanded bed adsorption chromatography

Detalhes bibliográficos
Autor(a) principal: Santos, Everaldo Silvino dos
Data de Publicação: 2014
Outros Autores: Santana, Sergio Carvalho de, Silva Filho, Raimundo Cosme da, Cavalcanti, Jorge dos Santos, Oliveira, Jackson Araujo de, Macedo, Gorete Ribeiro de, Padilha, Francine Ferreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/32529
Resumo: A mathematical model was developed to predict breakthrough curves during purification of the two chitosan- ases from Metarhizium anisopliae by expanded bed adsorption, taking into account the axial dispersion of liquid and using Streamline DEAE and SP XL adsorbents, anion and cation exchange resins, respectively. All the experiments were performed without clarification (with cells) aiming at the reduction of unit operations in future projects of separation processes, thereby reducing capital and operating costs. Chitosanases are enzymes that hydrolyze the carbohydrate chitosan, resulting in oligosaccharides that have many remarkable biological activities, such as anti-cancer, anti-HIV and antioxidant activities. The two adsorbents had similar performance in relation to hydrodynamics and mass transfer. The results of the parametric sensitivity analysis agree with the literature, and the model was validated with an average high degree of fit (94.68%) between simulated and experimental data obtained in this work
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spelling Santos, Everaldo Silvino dosSantana, Sergio Carvalho deSilva Filho, Raimundo Cosme daCavalcanti, Jorge dos SantosOliveira, Jackson Araujo deMacedo, Gorete Ribeiro dePadilha, Francine Ferreira2021-05-17T13:24:31Z2021-05-17T13:24:31Z2014-02-17Santana, S. C. ; SILVA FILHO, R. C. ; CAVALCANTI, J. S. ; OLIVEIRA, J. A. ; Macedo, Gorete R. ; Padilha, F. F ; SANTOS, E. S. . Modeling and simulation of breakthrough curves during purification of two chitosanases from Metarhizium anisopliae using ion-exchange with expanded bed adsorption chromatography. Korean Journal of Chemical Engineering (Print), v. 31, p. 684-691, 2014. Disponível em: https://link.springer.com/article/10.1007%2Fs11814-013-0269-3 Acesso em: 06 abr. 2021. https://doi.org/10.1007/s11814-013-0269-30256-11151975-7220https://repositorio.ufrn.br/handle/123456789/3252910.1007/s11814-013-0269-3Springer Nature SwitzerlandAttribution-NonCommercial 3.0 Brazilhttp://creativecommons.org/licenses/by-nc/3.0/br/info:eu-repo/semantics/openAccessModelingExpanded Bed AdsorptionPurificationChitosanaseMetarhizium anisopliaeModeling and simulation of breakthrough curves during purification of two chitosanases from Metarhizium anisopliae using ion-exchange with expanded bed adsorption chromatographyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleA mathematical model was developed to predict breakthrough curves during purification of the two chitosan- ases from Metarhizium anisopliae by expanded bed adsorption, taking into account the axial dispersion of liquid and using Streamline DEAE and SP XL adsorbents, anion and cation exchange resins, respectively. All the experiments were performed without clarification (with cells) aiming at the reduction of unit operations in future projects of separation processes, thereby reducing capital and operating costs. Chitosanases are enzymes that hydrolyze the carbohydrate chitosan, resulting in oligosaccharides that have many remarkable biological activities, such as anti-cancer, anti-HIV and antioxidant activities. The two adsorbents had similar performance in relation to hydrodynamics and mass transfer. The results of the parametric sensitivity analysis agree with the literature, and the model was validated with an average high degree of fit (94.68%) between simulated and experimental data obtained in this workengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8920https://repositorio.ufrn.br/bitstream/123456789/32529/2/license_rdf728dfda2fa81b274c619d08d1dfc1a03MD52ORIGINALModelingSimulationBreakthrough_Santos_2014 (1).pdfModelingSimulationBreakthrough_Santos_2014 (1).pdfapplication/pdf363473https://repositorio.ufrn.br/bitstream/123456789/32529/1/ModelingSimulationBreakthrough_Santos_2014%20%281%29.pdf855501a1a3dbf38ae3b65ae6fdc110a2MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/32529/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/325292021-05-17 10:24:33.499oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-05-17T13:24:33Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Modeling and simulation of breakthrough curves during purification of two chitosanases from Metarhizium anisopliae using ion-exchange with expanded bed adsorption chromatography
title Modeling and simulation of breakthrough curves during purification of two chitosanases from Metarhizium anisopliae using ion-exchange with expanded bed adsorption chromatography
spellingShingle Modeling and simulation of breakthrough curves during purification of two chitosanases from Metarhizium anisopliae using ion-exchange with expanded bed adsorption chromatography
Santos, Everaldo Silvino dos
Modeling
Expanded Bed Adsorption
Purification
Chitosanase
Metarhizium anisopliae
title_short Modeling and simulation of breakthrough curves during purification of two chitosanases from Metarhizium anisopliae using ion-exchange with expanded bed adsorption chromatography
title_full Modeling and simulation of breakthrough curves during purification of two chitosanases from Metarhizium anisopliae using ion-exchange with expanded bed adsorption chromatography
title_fullStr Modeling and simulation of breakthrough curves during purification of two chitosanases from Metarhizium anisopliae using ion-exchange with expanded bed adsorption chromatography
title_full_unstemmed Modeling and simulation of breakthrough curves during purification of two chitosanases from Metarhizium anisopliae using ion-exchange with expanded bed adsorption chromatography
title_sort Modeling and simulation of breakthrough curves during purification of two chitosanases from Metarhizium anisopliae using ion-exchange with expanded bed adsorption chromatography
author Santos, Everaldo Silvino dos
author_facet Santos, Everaldo Silvino dos
Santana, Sergio Carvalho de
Silva Filho, Raimundo Cosme da
Cavalcanti, Jorge dos Santos
Oliveira, Jackson Araujo de
Macedo, Gorete Ribeiro de
Padilha, Francine Ferreira
author_role author
author2 Santana, Sergio Carvalho de
Silva Filho, Raimundo Cosme da
Cavalcanti, Jorge dos Santos
Oliveira, Jackson Araujo de
Macedo, Gorete Ribeiro de
Padilha, Francine Ferreira
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Everaldo Silvino dos
Santana, Sergio Carvalho de
Silva Filho, Raimundo Cosme da
Cavalcanti, Jorge dos Santos
Oliveira, Jackson Araujo de
Macedo, Gorete Ribeiro de
Padilha, Francine Ferreira
dc.subject.por.fl_str_mv Modeling
Expanded Bed Adsorption
Purification
Chitosanase
Metarhizium anisopliae
topic Modeling
Expanded Bed Adsorption
Purification
Chitosanase
Metarhizium anisopliae
description A mathematical model was developed to predict breakthrough curves during purification of the two chitosan- ases from Metarhizium anisopliae by expanded bed adsorption, taking into account the axial dispersion of liquid and using Streamline DEAE and SP XL adsorbents, anion and cation exchange resins, respectively. All the experiments were performed without clarification (with cells) aiming at the reduction of unit operations in future projects of separation processes, thereby reducing capital and operating costs. Chitosanases are enzymes that hydrolyze the carbohydrate chitosan, resulting in oligosaccharides that have many remarkable biological activities, such as anti-cancer, anti-HIV and antioxidant activities. The two adsorbents had similar performance in relation to hydrodynamics and mass transfer. The results of the parametric sensitivity analysis agree with the literature, and the model was validated with an average high degree of fit (94.68%) between simulated and experimental data obtained in this work
publishDate 2014
dc.date.issued.fl_str_mv 2014-02-17
dc.date.accessioned.fl_str_mv 2021-05-17T13:24:31Z
dc.date.available.fl_str_mv 2021-05-17T13:24:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Santana, S. C. ; SILVA FILHO, R. C. ; CAVALCANTI, J. S. ; OLIVEIRA, J. A. ; Macedo, Gorete R. ; Padilha, F. F ; SANTOS, E. S. . Modeling and simulation of breakthrough curves during purification of two chitosanases from Metarhizium anisopliae using ion-exchange with expanded bed adsorption chromatography. Korean Journal of Chemical Engineering (Print), v. 31, p. 684-691, 2014. Disponível em: https://link.springer.com/article/10.1007%2Fs11814-013-0269-3 Acesso em: 06 abr. 2021. https://doi.org/10.1007/s11814-013-0269-3
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/32529
dc.identifier.issn.none.fl_str_mv 0256-1115
1975-7220
dc.identifier.doi.none.fl_str_mv 10.1007/s11814-013-0269-3
identifier_str_mv Santana, S. C. ; SILVA FILHO, R. C. ; CAVALCANTI, J. S. ; OLIVEIRA, J. A. ; Macedo, Gorete R. ; Padilha, F. F ; SANTOS, E. S. . Modeling and simulation of breakthrough curves during purification of two chitosanases from Metarhizium anisopliae using ion-exchange with expanded bed adsorption chromatography. Korean Journal of Chemical Engineering (Print), v. 31, p. 684-691, 2014. Disponível em: https://link.springer.com/article/10.1007%2Fs11814-013-0269-3 Acesso em: 06 abr. 2021. https://doi.org/10.1007/s11814-013-0269-3
0256-1115
1975-7220
10.1007/s11814-013-0269-3
url https://repositorio.ufrn.br/handle/123456789/32529
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language eng
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http://creativecommons.org/licenses/by-nc/3.0/br/
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rights_invalid_str_mv Attribution-NonCommercial 3.0 Brazil
http://creativecommons.org/licenses/by-nc/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature Switzerland
publisher.none.fl_str_mv Springer Nature Switzerland
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