Comparative digestibility and the inhibition of mammalian digestive enzymes from mature and immature cowpea (Vigna unguiculata (L.) Walp.) seeds

Detalhes bibliográficos
Autor(a) principal: Morais, Ana Heloneida de Araújo
Data de Publicação: 2004
Outros Autores: Lima, Liziane Maria de, Oliveira, Antônia Elenir Amâncio, Pereira, Railene, Miranda, Maria Raquel Alcântara de, Sales, Maurício Pereira de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/58141
https://doi.org/10.1016/S0956-7135(03)00021-5
Resumo: Globulins and albumins from mature cowpea seeds had inhibitory activity levels of 9.5 and 67.1 units trypsin inhibited per mg protein and globulins and albumins from immature seeds had trypsin inhibitory activity levels of 6.3 and 26.6 units trypsin inhibited per mg protein, respectively. Albumins from immature seeds had inhibitory activity level of 4 units salivary amylase inhibited per mg albumin. The globulins (native and heated) from both seeds were submitted to hydrolysis by trypsin and analyzed by SDS-PAGE. Native globulins from mature and immature seeds showed digestion products of ∼30 and ∼20 kDa, respectively. Trypsin strongly digested heated globulins from both kinds cowpeas
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spelling Morais, Ana Heloneida de AraújoLima, Liziane Maria deOliveira, Antônia Elenir AmâncioPereira, RaileneMiranda, Maria Raquel Alcântara deSales, Maurício Pereira de2024-04-12T21:36:58Z2024-04-12T21:36:58Z2004LIMA, Liziane Maria de; MORAIS, Ana Heloneida Araújo; OLIVEIRA, Antônia Elenir Amâncio; PEREIRA, Railene; MIRANDA, Maria Raquel Alcântara de; SALES, Maurício Pereira de. Comparative digestibility and the inhibition of mammalian digestive enzymes from mature and immature cowpea (Vigna unguiculata (L.) Walp.) seeds. Food Control, England, v. 15, p. 107-110, 2004. Disponível em: https://www.sciencedirect.com/science/article/pii/S0956713503000215?via%3Dihub. Acesso em: 12 abr. 2024.https://repositorio.ufrn.br/handle/123456789/58141https://doi.org/10.1016/S0956-7135(03)00021-5Food ControlMature and immature cowpeaVigna unguiculataGlobulin digestibilityTrypsin inhibitorsAmylase inhibitorsComparative digestibility and the inhibition of mammalian digestive enzymes from mature and immature cowpea (Vigna unguiculata (L.) Walp.) seedsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGlobulins and albumins from mature cowpea seeds had inhibitory activity levels of 9.5 and 67.1 units trypsin inhibited per mg protein and globulins and albumins from immature seeds had trypsin inhibitory activity levels of 6.3 and 26.6 units trypsin inhibited per mg protein, respectively. Albumins from immature seeds had inhibitory activity level of 4 units salivary amylase inhibited per mg albumin. The globulins (native and heated) from both seeds were submitted to hydrolysis by trypsin and analyzed by SDS-PAGE. Native globulins from mature and immature seeds showed digestion products of ∼30 and ∼20 kDa, respectively. Trypsin strongly digested heated globulins from both kinds cowpeasengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/58141/2/license.txte9597aa2854d128fd968be5edc8a28d9MD52123456789/581412024-04-12 18:37:17.367oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2024-04-12T21:37:17Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Comparative digestibility and the inhibition of mammalian digestive enzymes from mature and immature cowpea (Vigna unguiculata (L.) Walp.) seeds
title Comparative digestibility and the inhibition of mammalian digestive enzymes from mature and immature cowpea (Vigna unguiculata (L.) Walp.) seeds
spellingShingle Comparative digestibility and the inhibition of mammalian digestive enzymes from mature and immature cowpea (Vigna unguiculata (L.) Walp.) seeds
Morais, Ana Heloneida de Araújo
Mature and immature cowpea
Vigna unguiculata
Globulin digestibility
Trypsin inhibitors
Amylase inhibitors
title_short Comparative digestibility and the inhibition of mammalian digestive enzymes from mature and immature cowpea (Vigna unguiculata (L.) Walp.) seeds
title_full Comparative digestibility and the inhibition of mammalian digestive enzymes from mature and immature cowpea (Vigna unguiculata (L.) Walp.) seeds
title_fullStr Comparative digestibility and the inhibition of mammalian digestive enzymes from mature and immature cowpea (Vigna unguiculata (L.) Walp.) seeds
title_full_unstemmed Comparative digestibility and the inhibition of mammalian digestive enzymes from mature and immature cowpea (Vigna unguiculata (L.) Walp.) seeds
title_sort Comparative digestibility and the inhibition of mammalian digestive enzymes from mature and immature cowpea (Vigna unguiculata (L.) Walp.) seeds
author Morais, Ana Heloneida de Araújo
author_facet Morais, Ana Heloneida de Araújo
Lima, Liziane Maria de
Oliveira, Antônia Elenir Amâncio
Pereira, Railene
Miranda, Maria Raquel Alcântara de
Sales, Maurício Pereira de
author_role author
author2 Lima, Liziane Maria de
Oliveira, Antônia Elenir Amâncio
Pereira, Railene
Miranda, Maria Raquel Alcântara de
Sales, Maurício Pereira de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Morais, Ana Heloneida de Araújo
Lima, Liziane Maria de
Oliveira, Antônia Elenir Amâncio
Pereira, Railene
Miranda, Maria Raquel Alcântara de
Sales, Maurício Pereira de
dc.subject.por.fl_str_mv Mature and immature cowpea
Vigna unguiculata
Globulin digestibility
Trypsin inhibitors
Amylase inhibitors
topic Mature and immature cowpea
Vigna unguiculata
Globulin digestibility
Trypsin inhibitors
Amylase inhibitors
description Globulins and albumins from mature cowpea seeds had inhibitory activity levels of 9.5 and 67.1 units trypsin inhibited per mg protein and globulins and albumins from immature seeds had trypsin inhibitory activity levels of 6.3 and 26.6 units trypsin inhibited per mg protein, respectively. Albumins from immature seeds had inhibitory activity level of 4 units salivary amylase inhibited per mg albumin. The globulins (native and heated) from both seeds were submitted to hydrolysis by trypsin and analyzed by SDS-PAGE. Native globulins from mature and immature seeds showed digestion products of ∼30 and ∼20 kDa, respectively. Trypsin strongly digested heated globulins from both kinds cowpeas
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2024-04-12T21:36:58Z
dc.date.available.fl_str_mv 2024-04-12T21:36:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LIMA, Liziane Maria de; MORAIS, Ana Heloneida Araújo; OLIVEIRA, Antônia Elenir Amâncio; PEREIRA, Railene; MIRANDA, Maria Raquel Alcântara de; SALES, Maurício Pereira de. Comparative digestibility and the inhibition of mammalian digestive enzymes from mature and immature cowpea (Vigna unguiculata (L.) Walp.) seeds. Food Control, England, v. 15, p. 107-110, 2004. Disponível em: https://www.sciencedirect.com/science/article/pii/S0956713503000215?via%3Dihub. Acesso em: 12 abr. 2024.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/58141
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1016/S0956-7135(03)00021-5
identifier_str_mv LIMA, Liziane Maria de; MORAIS, Ana Heloneida Araújo; OLIVEIRA, Antônia Elenir Amâncio; PEREIRA, Railene; MIRANDA, Maria Raquel Alcântara de; SALES, Maurício Pereira de. Comparative digestibility and the inhibition of mammalian digestive enzymes from mature and immature cowpea (Vigna unguiculata (L.) Walp.) seeds. Food Control, England, v. 15, p. 107-110, 2004. Disponível em: https://www.sciencedirect.com/science/article/pii/S0956713503000215?via%3Dihub. Acesso em: 12 abr. 2024.
url https://repositorio.ufrn.br/handle/123456789/58141
https://doi.org/10.1016/S0956-7135(03)00021-5
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Food Control
publisher.none.fl_str_mv Food Control
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRN
instname:Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN
instname_str Universidade Federal do Rio Grande do Norte (UFRN)
instacron_str UFRN
institution UFRN
reponame_str Repositório Institucional da UFRN
collection Repositório Institucional da UFRN
bitstream.url.fl_str_mv https://repositorio.ufrn.br/bitstream/123456789/58141/2/license.txt
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repository.name.fl_str_mv Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)
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