Alteração da expressão de genes relacionados a doenças neurodegenerativas em modelos inflamatórios tratados com inibidores de APE1

• Autor(a) principal: Oliveira, Thais Teixeira
• Data de Publicação: 2017
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFRN
• Texto Completo: https://repositorio.ufrn.br/handle/123456789/43174
• topic: Estresse oxidativo; Oxidative stress; Neurodegeneração; Neurodegeneration; Mitocondria; Mitochondria

Inflammation is one of the main defense mechanisms against pathogens and antigens, but in the nervous system inflammation can lead to functional disturbance and death of neurons when it develops in a chronic or exacerbated way. Infectious diseases, such as meningitidis, which develop in the CNS often cause complications and neuronal death, many studies have already associated these complications with reactive oxygen species generated during inflammation. Due to the damage caused to DNA and its relation to neuronal death, our research group investigated the relationship of DNA repair enzymes with the occurrence of bacterial meningitis, in this study we observed that the occurrence of polymorphisms in genes of the bases excision repair was more frequent in patients with bacterial meningitis, in addition the amount of DNA damage was also higher in patients with APE1 and OGG1 polymorphism. To investigate the anti-inflammatory role of APE1, we performed a sequencing of the monocyte lineage RNA pool (U937) by observing gene expression alterations after the use of E3330 inhibitors Methoxyamine and resveratrol, one of the results was the appearance of many Genes belonging to neurodegeneration pathways with altered expression after the treatments. This work aimed to investigate these genes better; we observed that the treatment with E3330 and Resveratrol down regulates many mitochondrial genes involved in the pathogenesis of diseases such as Parkinson and Alzheimer.