Essential Saccharomyces cerevisiae genome instability suppressing genes identify potential human tumor suppressors

Detalhes bibliográficos
Autor(a) principal: Srivatsan, Anjana
Data de Publicação: 2019
Outros Autores: Li, Binzhong, Sanchez, Dafne N., Somach, Steven B., Silva, Vandeclecio L. da, Souza, Sandro José de, Putnam, Christopher D., Kolodner, Richard D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/jspui/handle/123456789/27645
https://doi.org/10.1073/pnas.1906921116
Resumo: Gross Chromosomal Rearrangements (GCRs) play an important role in human diseases, including cancer. Although most of the nonessential Genome Instability Suppressing (GIS) genes in Saccharomyces cerevisiae are known, the essential genes in which mutations can cause increased GCR rates are not well understood. Here 2 S. cerevisiae GCR assays were used to screen a targeted collection of temperature-sensitive mutants to identify mutations that caused increased GCR rates. This identified 94 essential GIS (eGIS) genes in which mutations cause increased GCR rates and 38 candidate eGIS genes that encode eGIS1 protein-interacting or family member proteins. Analysis of TCGA data using the human genes predicted to encode the proteins and protein complexes implicated by the S. cerevisiae eGIS genes revealed a significant enrichment of mutations affecting predicted human eGIS genes in 10 of the 16 cancers analyzed.
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spelling Srivatsan, AnjanaLi, BinzhongSanchez, Dafne N.Somach, Steven B.Silva, Vandeclecio L. daSouza, Sandro José dePutnam, Christopher D.Kolodner, Richard D.2019-09-05T16:57:11Z2019-09-05T16:57:11Z2019-08SRIVATSAN, A.; LI, B.; SANCHEZ, D. N.; SOMACH, S. B.; SILVA, V. L.; SOUZA, S. J.; PUTNAM, C. D.; KOLODNER, R. D. Essential Saccharomyces cerevisiae genome instability suppressing genes identify potential human tumor suppressors. Proceedings of the National Academy of Sciences, [s. l.], v. 116, n. 35, p. 17377-17382, ago. 2019. DOI: https://doi.org/10.1073/pnas.1906921116. Disponível em: https://www.pnas.org/content/116/35/17377.long. Acesso em: 05 set. 2019.https://repositorio.ufrn.br/jspui/handle/123456789/27645https://doi.org/10.1073/pnas.1906921116Genome instabilitychromosome dynamics and replicationcancerEssential Saccharomyces cerevisiae genome instability suppressing genes identify potential human tumor suppressorsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGross Chromosomal Rearrangements (GCRs) play an important role in human diseases, including cancer. Although most of the nonessential Genome Instability Suppressing (GIS) genes in Saccharomyces cerevisiae are known, the essential genes in which mutations can cause increased GCR rates are not well understood. Here 2 S. cerevisiae GCR assays were used to screen a targeted collection of temperature-sensitive mutants to identify mutations that caused increased GCR rates. This identified 94 essential GIS (eGIS) genes in which mutations cause increased GCR rates and 38 candidate eGIS genes that encode eGIS1 protein-interacting or family member proteins. Analysis of TCGA data using the human genes predicted to encode the proteins and protein complexes implicated by the S. cerevisiae eGIS genes revealed a significant enrichment of mutations affecting predicted human eGIS genes in 10 of the 16 cancers analyzed.engreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessORIGINALSandroSouza_ICe_2019_Essential Saccharomyces cerevisiae.pdfSandroSouza_ICe_2019_Essential Saccharomyces cerevisiae.pdfSandroSouza_ICe_2019_Essential Saccharomyces cerevisiaeapplication/pdf846227https://repositorio.ufrn.br/bitstream/123456789/27645/1/SandroSouza_ICe_2019_Essential%20Saccharomyces%20cerevisiae.pdfde1d91ca25699bf746b4579041396acbMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/27645/2/license.txte9597aa2854d128fd968be5edc8a28d9MD52TEXTSandroSouza_ICe_2019_Essential Saccharomyces cerevisiae.pdf.txtSandroSouza_ICe_2019_Essential Saccharomyces cerevisiae.pdf.txtExtracted texttext/plain48408https://repositorio.ufrn.br/bitstream/123456789/27645/3/SandroSouza_ICe_2019_Essential%20Saccharomyces%20cerevisiae.pdf.txt1ced53e383fc62cfdecea264c2f1489aMD53THUMBNAILSandroSouza_ICe_2019_Essential Saccharomyces cerevisiae.pdf.jpgSandroSouza_ICe_2019_Essential Saccharomyces cerevisiae.pdf.jpgGenerated Thumbnailimage/jpeg2040https://repositorio.ufrn.br/bitstream/123456789/27645/4/SandroSouza_ICe_2019_Essential%20Saccharomyces%20cerevisiae.pdf.jpgf8b3f915a1def9260212a3714273db1aMD54123456789/276452019-09-08 02:16:58.378oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2019-09-08T05:16:58Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Essential Saccharomyces cerevisiae genome instability suppressing genes identify potential human tumor suppressors
title Essential Saccharomyces cerevisiae genome instability suppressing genes identify potential human tumor suppressors
spellingShingle Essential Saccharomyces cerevisiae genome instability suppressing genes identify potential human tumor suppressors
Srivatsan, Anjana
Genome instability
chromosome dynamics and replication
cancer
title_short Essential Saccharomyces cerevisiae genome instability suppressing genes identify potential human tumor suppressors
title_full Essential Saccharomyces cerevisiae genome instability suppressing genes identify potential human tumor suppressors
title_fullStr Essential Saccharomyces cerevisiae genome instability suppressing genes identify potential human tumor suppressors
title_full_unstemmed Essential Saccharomyces cerevisiae genome instability suppressing genes identify potential human tumor suppressors
title_sort Essential Saccharomyces cerevisiae genome instability suppressing genes identify potential human tumor suppressors
author Srivatsan, Anjana
author_facet Srivatsan, Anjana
Li, Binzhong
Sanchez, Dafne N.
Somach, Steven B.
Silva, Vandeclecio L. da
Souza, Sandro José de
Putnam, Christopher D.
Kolodner, Richard D.
author_role author
author2 Li, Binzhong
Sanchez, Dafne N.
Somach, Steven B.
Silva, Vandeclecio L. da
Souza, Sandro José de
Putnam, Christopher D.
Kolodner, Richard D.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Srivatsan, Anjana
Li, Binzhong
Sanchez, Dafne N.
Somach, Steven B.
Silva, Vandeclecio L. da
Souza, Sandro José de
Putnam, Christopher D.
Kolodner, Richard D.
dc.subject.por.fl_str_mv Genome instability
chromosome dynamics and replication
cancer
topic Genome instability
chromosome dynamics and replication
cancer
description Gross Chromosomal Rearrangements (GCRs) play an important role in human diseases, including cancer. Although most of the nonessential Genome Instability Suppressing (GIS) genes in Saccharomyces cerevisiae are known, the essential genes in which mutations can cause increased GCR rates are not well understood. Here 2 S. cerevisiae GCR assays were used to screen a targeted collection of temperature-sensitive mutants to identify mutations that caused increased GCR rates. This identified 94 essential GIS (eGIS) genes in which mutations cause increased GCR rates and 38 candidate eGIS genes that encode eGIS1 protein-interacting or family member proteins. Analysis of TCGA data using the human genes predicted to encode the proteins and protein complexes implicated by the S. cerevisiae eGIS genes revealed a significant enrichment of mutations affecting predicted human eGIS genes in 10 of the 16 cancers analyzed.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-09-05T16:57:11Z
dc.date.available.fl_str_mv 2019-09-05T16:57:11Z
dc.date.issued.fl_str_mv 2019-08
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SRIVATSAN, A.; LI, B.; SANCHEZ, D. N.; SOMACH, S. B.; SILVA, V. L.; SOUZA, S. J.; PUTNAM, C. D.; KOLODNER, R. D. Essential Saccharomyces cerevisiae genome instability suppressing genes identify potential human tumor suppressors. Proceedings of the National Academy of Sciences, [s. l.], v. 116, n. 35, p. 17377-17382, ago. 2019. DOI: https://doi.org/10.1073/pnas.1906921116. Disponível em: https://www.pnas.org/content/116/35/17377.long. Acesso em: 05 set. 2019.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/jspui/handle/123456789/27645
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1073/pnas.1906921116
identifier_str_mv SRIVATSAN, A.; LI, B.; SANCHEZ, D. N.; SOMACH, S. B.; SILVA, V. L.; SOUZA, S. J.; PUTNAM, C. D.; KOLODNER, R. D. Essential Saccharomyces cerevisiae genome instability suppressing genes identify potential human tumor suppressors. Proceedings of the National Academy of Sciences, [s. l.], v. 116, n. 35, p. 17377-17382, ago. 2019. DOI: https://doi.org/10.1073/pnas.1906921116. Disponível em: https://www.pnas.org/content/116/35/17377.long. Acesso em: 05 set. 2019.
url https://repositorio.ufrn.br/jspui/handle/123456789/27645
https://doi.org/10.1073/pnas.1906921116
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