Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case-control study in southeastern Brazilians

Detalhes bibliográficos
Autor(a) principal: Barbosa, Andressa
Data de Publicação: 2016
Outros Autores: Santos, Marcelo dos, Podestá, José Roberto Vasconcelos de, Gouvêa, Sônia Alves, Zeidler, Sandra Ventorin Von, Louro, Iúri Drumond, Cordeiro-Silva, Melissa de Freitas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/31560
Resumo: Introduction: Oral squamous cell carcinoma (OSCC) is a serious public health problem, due to its high mortality rate and worldwide rising incidence. OSCC susceptibility is mediated by interactions between genetic and environmental factors. Studies suggest that genetic variants encoding enzymes involved in folate metabolism may modulate OSCC risk by altering DNA synthesis/repair and methylation process. Objective: The goals of this study were to evaluate the association of three genotypic polymorphism (MTHFR C677T, MTHFR A1298C and CBS 844ins68) and oral cancer risk in southeastern Brazilians and evaluate the interactions between polymorphisms and clinical histopathological parameters. Methods: This case-control study included 101 cases and 102 controls in the state of Espírito Santo, Brazil. MTHFR genotyping was done by PCR-RFLP (polymerase chain reaction --- restriction fragment length polymorphism) and CBS genotyping by PCR (polymerase chain reaction) analysis
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spelling Barbosa, AndressaSantos, Marcelo dosPodestá, José Roberto Vasconcelos deGouvêa, Sônia AlvesZeidler, Sandra Ventorin VonLouro, Iúri DrumondCordeiro-Silva, Melissa de Freitas2021-02-19T10:40:37Z2021-02-19T10:40:37Z2016BARBOSA, Andressa; SANTOS, Marcelo dos; PODESTA, José Roberto Vasconcelos de; GOUVEA, Sônia Alves; ZEINDLER, Sandra Ventorin Von; LOURO, Iúri Drumond; CORDEIRO-SILVA, Melissa de Freitas. Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case - control study in southeastern Brazilians. Brazilian Journal of Otorhinolaryngology, [s. l.], v. 82, n. 5, p. 558-566, 2016. Disponível em: https://www.sciencedirect.com/science/article/pii/S1808869415002384. Acesso em: 07 jul. 2020. https://doi.org/10.1016/j.bjorl.2015.10.0121808-8694 (print)https://repositorio.ufrn.br/handle/123456789/3156010.1016/j.bjorl.2015.10.012Introduction: Oral squamous cell carcinoma (OSCC) is a serious public health problem, due to its high mortality rate and worldwide rising incidence. OSCC susceptibility is mediated by interactions between genetic and environmental factors. Studies suggest that genetic variants encoding enzymes involved in folate metabolism may modulate OSCC risk by altering DNA synthesis/repair and methylation process. Objective: The goals of this study were to evaluate the association of three genotypic polymorphism (MTHFR C677T, MTHFR A1298C and CBS 844ins68) and oral cancer risk in southeastern Brazilians and evaluate the interactions between polymorphisms and clinical histopathological parameters. Methods: This case-control study included 101 cases and 102 controls in the state of Espírito Santo, Brazil. MTHFR genotyping was done by PCR-RFLP (polymerase chain reaction --- restriction fragment length polymorphism) and CBS genotyping by PCR (polymerase chain reaction) analysişIntrodução: O carcinoma espinocelular oral (CECO) trata-se de um importante problema de saúde pública, devido à elevada taxa de mortalidade e incidência crescente em todo o mundo. A susceptibilidade ao CECO é medida por interações entre fatores genéticos e ambientais. Estudos sugerem que as variantes genéticas que codificam as enzimas envolvidas no metabolismo do folato podem modular o risco de CECO, alterando a síntese/reparação do DNA e o processo de metilação. Objetivo: Os objetivos deste estudo foram avaliar a associação de três polimorfismos genótipos (MTHFR C677T, MTHFR A1298C e CBS 844ins68) e o risco de câncer bucal em brasileiros da região Sudeste, e avaliar as interações entre polimorfismos e parâmetros clínico-histopatológicos. Método: Este estudo de caso-controle incluiu 101 casos e 102 controles no Estado do Espírito Santo, Brasil. A genotipagem do polimorfismo MTHFR foi realizado por PCR-RFLP (Reação de Polimerase em Cadeia – Polimorfismo no Comprimento de Fragmento de Restrição) e a do CBS por análise da PCR (Reação de Polimerase em Cadeia). Resultados: O polimorfismo MTHFR C677T foi associado ao envolvimento de gânglios linfáticos. O genótipo CT + TT atuou como um fator protetor. O genótipo MTHFR A1298C AC + CC foi associado à diferenciação do tumor e, possivelmente, a um prognóstico melhor. Na análise de risco, a correlação entre os genótipos e o CECO não foi observada. Conclusão: Concluímos que os polimorfismos MTHFR C677T, MTHFR A1298C e CBS 844ins68 não estão associados ao risco de CECO nos brasileiros da região Sudeste; no entanto, sugerimos um efeito prognóstico associado aos polimorfismos MTHFR C677T e A1298C em CECO.ElsevierAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessOral squamous cell carcinomaMethylenetetrahydrofolate reductaseCystathionine beta-synthaseGenetic polymorphismCarcinoma epidermoide oralMetilenotetrahidrofolato redutaseCistationina beta-sintasePolimorfismo genéticoPolymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case-control study in southeastern BraziliansPolimorfismos em metilenotetrahidrofolato redutase, cistationina beta-sintase no câncer de boca - um estudo de caso-controle no Sudeste brasileiroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNLICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/31560/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53ORIGINALPolymorphismsMethylenetetrahydrofolateReductase_Santos_2016.pdfPolymorphismsMethylenetetrahydrofolateReductase_Santos_2016.pdfapplication/pdf701397https://repositorio.ufrn.br/bitstream/123456789/31560/1/PolymorphismsMethylenetetrahydrofolateReductase_Santos_2016.pdf661cbaad057730c362f28491290c7b4aMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/31560/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52TEXTPolymorphismsMethylenetetrahydrofolateReductase_Santos_2016.pdf.txtPolymorphismsMethylenetetrahydrofolateReductase_Santos_2016.pdf.txtExtracted texttext/plain39647https://repositorio.ufrn.br/bitstream/123456789/31560/4/PolymorphismsMethylenetetrahydrofolateReductase_Santos_2016.pdf.txt0e27ec3afd96598856bdbb69adbaa369MD54THUMBNAILPolymorphismsMethylenetetrahydrofolateReductase_Santos_2016.pdf.jpgPolymorphismsMethylenetetrahydrofolateReductase_Santos_2016.pdf.jpgGenerated Thumbnailimage/jpeg1841https://repositorio.ufrn.br/bitstream/123456789/31560/5/PolymorphismsMethylenetetrahydrofolateReductase_Santos_2016.pdf.jpg125cd2fa3387e86f93a38d1725047f36MD55123456789/315602021-02-21 05:32:23.932oai:https://repositorio.ufrn.br:123456789/31560Tk9OLUVYQ0xVU0lWRSBESVNUUklCVVRJT04gTElDRU5TRQoKCkJ5IHNpZ25pbmcgYW5kIGRlbGl2ZXJpbmcgdGhpcyBsaWNlbnNlLCBNci4gKGF1dGhvciBvciBjb3B5cmlnaHQgaG9sZGVyKToKCgphKSBHcmFudHMgdGhlIFVuaXZlcnNpZGFkZSBGZWRlcmFsIFJpbyBHcmFuZGUgZG8gTm9ydGUgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgb2YKcmVwcm9kdWNlLCBjb252ZXJ0IChhcyBkZWZpbmVkIGJlbG93KSwgY29tbXVuaWNhdGUgYW5kIC8gb3IKZGlzdHJpYnV0ZSB0aGUgZGVsaXZlcmVkIGRvY3VtZW50IChpbmNsdWRpbmcgYWJzdHJhY3QgLyBhYnN0cmFjdCkgaW4KZGlnaXRhbCBvciBwcmludGVkIGZvcm1hdCBhbmQgaW4gYW55IG1lZGl1bS4KCmIpIERlY2xhcmVzIHRoYXQgdGhlIGRvY3VtZW50IHN1Ym1pdHRlZCBpcyBpdHMgb3JpZ2luYWwgd29yaywgYW5kIHRoYXQKeW91IGhhdmUgdGhlIHJpZ2h0IHRvIGdyYW50IHRoZSByaWdodHMgY29udGFpbmVkIGluIHRoaXMgbGljZW5zZS4gRGVjbGFyZXMKdGhhdCB0aGUgZGVsaXZlcnkgb2YgdGhlIGRvY3VtZW50IGRvZXMgbm90IGluZnJpbmdlLCBhcyBmYXIgYXMgaXQgaXMKdGhlIHJpZ2h0cyBvZiBhbnkgb3RoZXIgcGVyc29uIG9yIGVudGl0eS4KCmMpIElmIHRoZSBkb2N1bWVudCBkZWxpdmVyZWQgY29udGFpbnMgbWF0ZXJpYWwgd2hpY2ggZG9lcyBub3QKcmlnaHRzLCBkZWNsYXJlcyB0aGF0IGl0IGhhcyBvYnRhaW5lZCBhdXRob3JpemF0aW9uIGZyb20gdGhlIGhvbGRlciBvZiB0aGUKY29weXJpZ2h0IHRvIGdyYW50IHRoZSBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkbyBSaW8gR3JhbmRlIGRvIE5vcnRlIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdCB0aGlzIG1hdGVyaWFsIHdob3NlIHJpZ2h0cyBhcmUgb2YKdGhpcmQgcGFydGllcyBpcyBjbGVhcmx5IGlkZW50aWZpZWQgYW5kIHJlY29nbml6ZWQgaW4gdGhlIHRleHQgb3IKY29udGVudCBvZiB0aGUgZG9jdW1lbnQgZGVsaXZlcmVkLgoKSWYgdGhlIGRvY3VtZW50IHN1Ym1pdHRlZCBpcyBiYXNlZCBvbiBmdW5kZWQgb3Igc3VwcG9ydGVkIHdvcmsKYnkgYW5vdGhlciBpbnN0aXR1dGlvbiBvdGhlciB0aGFuIHRoZSBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkbyBSaW8gR3JhbmRlIGRvIE5vcnRlLCBkZWNsYXJlcyB0aGF0IGl0IGhhcyBmdWxmaWxsZWQgYW55IG9ibGlnYXRpb25zIHJlcXVpcmVkIGJ5IHRoZSByZXNwZWN0aXZlIGFncmVlbWVudCBvciBhZ3JlZW1lbnQuCgpUaGUgVW5pdmVyc2lkYWRlIEZlZGVyYWwgZG8gUmlvIEdyYW5kZSBkbyBOb3J0ZSB3aWxsIGNsZWFybHkgaWRlbnRpZnkgaXRzIG5hbWUgKHMpIGFzIHRoZSBhdXRob3IgKHMpIG9yIGhvbGRlciAocykgb2YgdGhlIGRvY3VtZW50J3MgcmlnaHRzCmRlbGl2ZXJlZCwgYW5kIHdpbGwgbm90IG1ha2UgYW55IGNoYW5nZXMsIG90aGVyIHRoYW4gdGhvc2UgcGVybWl0dGVkIGJ5CnRoaXMgbGljZW5zZQo=Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-02-21T08:32:23Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case-control study in southeastern Brazilians
dc.title.alternative.pt_BR.fl_str_mv Polimorfismos em metilenotetrahidrofolato redutase, cistationina beta-sintase no câncer de boca - um estudo de caso-controle no Sudeste brasileiro
title Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case-control study in southeastern Brazilians
spellingShingle Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case-control study in southeastern Brazilians
Barbosa, Andressa
Oral squamous cell carcinoma
Methylenetetrahydrofolate reductase
Cystathionine beta-synthase
Genetic polymorphism
Carcinoma epidermoide oral
Metilenotetrahidrofolato redutase
Cistationina beta-sintase
Polimorfismo genético
title_short Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case-control study in southeastern Brazilians
title_full Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case-control study in southeastern Brazilians
title_fullStr Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case-control study in southeastern Brazilians
title_full_unstemmed Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case-control study in southeastern Brazilians
title_sort Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case-control study in southeastern Brazilians
author Barbosa, Andressa
author_facet Barbosa, Andressa
Santos, Marcelo dos
Podestá, José Roberto Vasconcelos de
Gouvêa, Sônia Alves
Zeidler, Sandra Ventorin Von
Louro, Iúri Drumond
Cordeiro-Silva, Melissa de Freitas
author_role author
author2 Santos, Marcelo dos
Podestá, José Roberto Vasconcelos de
Gouvêa, Sônia Alves
Zeidler, Sandra Ventorin Von
Louro, Iúri Drumond
Cordeiro-Silva, Melissa de Freitas
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barbosa, Andressa
Santos, Marcelo dos
Podestá, José Roberto Vasconcelos de
Gouvêa, Sônia Alves
Zeidler, Sandra Ventorin Von
Louro, Iúri Drumond
Cordeiro-Silva, Melissa de Freitas
dc.subject.por.fl_str_mv Oral squamous cell carcinoma
Methylenetetrahydrofolate reductase
Cystathionine beta-synthase
Genetic polymorphism
Carcinoma epidermoide oral
Metilenotetrahidrofolato redutase
Cistationina beta-sintase
Polimorfismo genético
topic Oral squamous cell carcinoma
Methylenetetrahydrofolate reductase
Cystathionine beta-synthase
Genetic polymorphism
Carcinoma epidermoide oral
Metilenotetrahidrofolato redutase
Cistationina beta-sintase
Polimorfismo genético
description Introduction: Oral squamous cell carcinoma (OSCC) is a serious public health problem, due to its high mortality rate and worldwide rising incidence. OSCC susceptibility is mediated by interactions between genetic and environmental factors. Studies suggest that genetic variants encoding enzymes involved in folate metabolism may modulate OSCC risk by altering DNA synthesis/repair and methylation process. Objective: The goals of this study were to evaluate the association of three genotypic polymorphism (MTHFR C677T, MTHFR A1298C and CBS 844ins68) and oral cancer risk in southeastern Brazilians and evaluate the interactions between polymorphisms and clinical histopathological parameters. Methods: This case-control study included 101 cases and 102 controls in the state of Espírito Santo, Brazil. MTHFR genotyping was done by PCR-RFLP (polymerase chain reaction --- restriction fragment length polymorphism) and CBS genotyping by PCR (polymerase chain reaction) analysis
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2021-02-19T10:40:37Z
dc.date.available.fl_str_mv 2021-02-19T10:40:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv BARBOSA, Andressa; SANTOS, Marcelo dos; PODESTA, José Roberto Vasconcelos de; GOUVEA, Sônia Alves; ZEINDLER, Sandra Ventorin Von; LOURO, Iúri Drumond; CORDEIRO-SILVA, Melissa de Freitas. Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case - control study in southeastern Brazilians. Brazilian Journal of Otorhinolaryngology, [s. l.], v. 82, n. 5, p. 558-566, 2016. Disponível em: https://www.sciencedirect.com/science/article/pii/S1808869415002384. Acesso em: 07 jul. 2020. https://doi.org/10.1016/j.bjorl.2015.10.012
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/31560
dc.identifier.issn.none.fl_str_mv 1808-8694 (print)
dc.identifier.doi.none.fl_str_mv 10.1016/j.bjorl.2015.10.012
identifier_str_mv BARBOSA, Andressa; SANTOS, Marcelo dos; PODESTA, José Roberto Vasconcelos de; GOUVEA, Sônia Alves; ZEINDLER, Sandra Ventorin Von; LOURO, Iúri Drumond; CORDEIRO-SILVA, Melissa de Freitas. Polymorphisms in methylenetetrahydrofolate reductase and cystathionine beta-synthase in oral cancer - a case - control study in southeastern Brazilians. Brazilian Journal of Otorhinolaryngology, [s. l.], v. 82, n. 5, p. 558-566, 2016. Disponível em: https://www.sciencedirect.com/science/article/pii/S1808869415002384. Acesso em: 07 jul. 2020. https://doi.org/10.1016/j.bjorl.2015.10.012
1808-8694 (print)
10.1016/j.bjorl.2015.10.012
url https://repositorio.ufrn.br/handle/123456789/31560
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 3.0 Brazil
http://creativecommons.org/licenses/by/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 3.0 Brazil
http://creativecommons.org/licenses/by/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
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