Preparation, characterization, and pharmacological activity of Cymbopogon winterianus Jowitt ex Bor (Poaceae) leaf essential oil of B-cyclodextrin inclusion complexes

• Autor(a) principal: Santos, Priscila Laíse dos
• Data de Publicação: 2015
• Outros Autores: Araujo, Adriano Antunes de Souza, Quintans, Jullyana de Souza Siqueira, Brito, Renan Guedes de, Serafini, Mairim Russo, Menezes, Paula dos Passos, Santos, Márcio Roberto Viana dos, Alves, Péricles Barreto, Lucca Junior, Waldecy de, Blank, Arie Fitzgerald, La Rocca, Viviana, Almeida, Reinaldo Nobrega de, Quintans Júnior, Lucindo José
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFS
• Texto Completo: http://ri.ufs.br/jspui/handle/riufs/7314
• topic: Cymbopogon winterianus; Essential oils; β-cyclodextrin; Óleos essenciais; β-ciclodextrina

This study aimed to evaluate the orofacial antinociceptive effect of the Cymbopogon winterianus essential oil (LEO) complexed in B-cyclodextrin (LEO-CD) and to assess the possible involvement of the central nervous system (CNS). The LEO was extracted, chromatographed, and complexed in B-cyclodextrin. The complex was characterized by differential scanning calorimetry (DSC) and thermogravimetry derivative (TG/DTG). Male Swiss mice (2-3 months) were treated with LEO-CD (50–200 mg/kg, p.o.), vehicle (distilled water, p.o.), or standard drug (i.p.) and subjected to the orofacial nociception formalin-, capsaicin-, and glutamateinduced. After the formalin test, the animals were perfused and the brains subjected to immunofluorescence for Fos. The rota-rod test (7 rpm/min) was carried out. Geraniol (37.57%) was the main compound of LEO. DSC and TG/DTG proved the complexation. The orofacial nociceptive behavior was significantly (p < 0.05) reduced. The number of Fos-positive cells was significantly changed in the dorsal raphe nucleus (p < 0.01), locus coeruleus (p < 0.001), trigeminal nucleus (p < 0.05), and trigeminal thalamic tract (p < 0.05). LEO-CD did not cause changes in motor coordination in the rota-rod test. Thus, our results suggested that LEO-CD has an orofacial antinociceptive profile, probably mediated by the activation of the CNS without changing the motor coordination.