Efeito do α-felandreno complexado e não complexado em hidroxipropil-β-ciclodextrina na nocicepção orofacial em roedores
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | http://ri.ufs.br/jspui/handle/riufs/16820 |
Resumo: | Objective: the work aims to expand the studies involving the pharmacological effects of the αphelandrene monoterpene, specifically in the promotion of orofacial antinociception. Methods: a hydroxypropil-β-cyclodextrin (HPβ-CD) complex was prepared with incorporation of the α-felandorene that was later physically characterized through high efficiency liquid chromatography (CLAE) and differential exploratory calorimetry (DSC). Seeking to evaluate the possible analgesic action of α-felandreno in animal models in orofacial nociception, the animals were divided into four groups (n = 8 per group) and treated with unlapled α-felandreno (FEN) at the 50mg/kg oral dose , α-felandreno complex in hydroxipropil-β-cyclodextrin (FENHPβCD) at a dose of 50mg/kg oral via, negative control (tween 80 0.2% in water) 10ml/kg per gavage and positive controls (morphine 10mg/kg i.p. For tests of formaline, glutamate and hypertonic saline; camphor 7.6mg/kg i.p. for the scine test and, diazepam 3mg/kg i.p. for the rod rod test). Finally, quantification of TNF-α and IL-1β cytokines was made in the Vibrissa region after the formaline test and animal locomotor analysis analysis through the motor coordination test and data collection for statistical evaluations. Results: in the α-felandorereno quantification test by Clae, a 5.8 minutes retention time was found and a peak of good chromatographic resolution, the correlation coefficient R2 = 0.9998 proves the linearity of the method and the limit results Detection (LD) and quantification (LQ) (0.81 and 2.44 µg/mL, respectively) demonstrated the sensitivity of the method. DSC results indicated greater α-felandreno stability when incorporated into HPβ-CD. In formalin-induced nociception, a significant antinociceptive effect was observed in animals treated with FEN and FEN-HPβCD 50mg/kg when compared with the vehicle in both the neurogenic (p<0.001 for both) and inflammatory (p<0.001 and p<005, respectively) phases, parallel to this, there was an inhibition of the cytokines TNF-α (p<0.05 and p<0.01, respectively) and IL-1β (p<0.01 and p<0.05, respectively). The treatment with FEN and FEN-HPβCD 50mg/kg when compared to the vehicle significantly reduced the nociception induced by cinnamaldehyde (p<0.01 and p<0.001, respectively), glutamate (p<0.001 and p<0.01, respectively) and hypertonic saline (p<0.05). In the motor coordination test done through the Rota-Rod apparatus, no behaviors were observed that influenced the locomotor activity of rodents, as animals treated with Fen and FEN-HPβCD 50mg/kg remained in the bar for more than 170 seconds in Every time (30, 60 and 120 minutes). Conclusion: it can then be concluded that α-felandorene monoterpene has antinociceptive effect, probably involving TRPA1, TRPA1, glutamatergic receptors and release of TNF-α and IL-1β cytokines. |
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Machado, Brennda GonzagaAntoniolli, Ângelo RobertoQuintans, Jullyana de Souza Siqueira2022-11-24T14:34:23Z2022-11-24T14:34:23Z2022-08-24MACHADO, Brennda Gonzaga. Efeito do α-felandreno complexado e não complexado em hidroxipropil-β-ciclodextrina na nocicepção orofacial em roedores. 2022. 58 f. Dissertação (Mestrado em Ciências Farmacêuticas) – Universidade Federal de Sergipe, São Cristóvão, 2022.http://ri.ufs.br/jspui/handle/riufs/16820Objective: the work aims to expand the studies involving the pharmacological effects of the αphelandrene monoterpene, specifically in the promotion of orofacial antinociception. Methods: a hydroxypropil-β-cyclodextrin (HPβ-CD) complex was prepared with incorporation of the α-felandorene that was later physically characterized through high efficiency liquid chromatography (CLAE) and differential exploratory calorimetry (DSC). Seeking to evaluate the possible analgesic action of α-felandreno in animal models in orofacial nociception, the animals were divided into four groups (n = 8 per group) and treated with unlapled α-felandreno (FEN) at the 50mg/kg oral dose , α-felandreno complex in hydroxipropil-β-cyclodextrin (FENHPβCD) at a dose of 50mg/kg oral via, negative control (tween 80 0.2% in water) 10ml/kg per gavage and positive controls (morphine 10mg/kg i.p. For tests of formaline, glutamate and hypertonic saline; camphor 7.6mg/kg i.p. for the scine test and, diazepam 3mg/kg i.p. for the rod rod test). Finally, quantification of TNF-α and IL-1β cytokines was made in the Vibrissa region after the formaline test and animal locomotor analysis analysis through the motor coordination test and data collection for statistical evaluations. Results: in the α-felandorereno quantification test by Clae, a 5.8 minutes retention time was found and a peak of good chromatographic resolution, the correlation coefficient R2 = 0.9998 proves the linearity of the method and the limit results Detection (LD) and quantification (LQ) (0.81 and 2.44 µg/mL, respectively) demonstrated the sensitivity of the method. DSC results indicated greater α-felandreno stability when incorporated into HPβ-CD. In formalin-induced nociception, a significant antinociceptive effect was observed in animals treated with FEN and FEN-HPβCD 50mg/kg when compared with the vehicle in both the neurogenic (p<0.001 for both) and inflammatory (p<0.001 and p<005, respectively) phases, parallel to this, there was an inhibition of the cytokines TNF-α (p<0.05 and p<0.01, respectively) and IL-1β (p<0.01 and p<0.05, respectively). The treatment with FEN and FEN-HPβCD 50mg/kg when compared to the vehicle significantly reduced the nociception induced by cinnamaldehyde (p<0.01 and p<0.001, respectively), glutamate (p<0.001 and p<0.01, respectively) and hypertonic saline (p<0.05). In the motor coordination test done through the Rota-Rod apparatus, no behaviors were observed that influenced the locomotor activity of rodents, as animals treated with Fen and FEN-HPβCD 50mg/kg remained in the bar for more than 170 seconds in Every time (30, 60 and 120 minutes). Conclusion: it can then be concluded that α-felandorene monoterpene has antinociceptive effect, probably involving TRPA1, TRPA1, glutamatergic receptors and release of TNF-α and IL-1β cytokines.Objetivo: o trabalho visa ampliar os estudos envolvendo efeitos farmacológicos do monoterpeno α-felandreno, especificamente na promoção da antinocicepção orofacial. Métodos: foi preparado um complexo de hidroxipropil-β-ciclodextrina (HPβ-CD) com incorporação do α-felandreno que posteriormente foi caracterizado físico quimicamente por meio dos testes Cromatografia líquida de alta eficiência (CLAE) e calorimetria exploratória diferencial (DSC). Para avaliar a ação analgésica em modelos animais de nocicepção orofacial, os animais foram divididos em quatro grupos (n = 8 por grupo) e tratados com α-felandreno não complexado (FEN) na dose de 50mg/kg via oral, α-felandreno complexado em hidroxipropil-β-ciclodextrina (FEN-HPβCD) na dose de 50mg/kg via oral, controle negativo (tween 80 0,2% em água) 10mL/kg por gavagem e controles positivos (Morfina 10mg/kg i.p. para os testes da formalina, glutamato e salina hipertônica; cânfora 7,6mg/kg i.p. para o teste do cinamaldeído e, diazepam 3mg/kg i.p. para o teste do rota-rod). A quantificação das citocinas TNF-α e IL-1β na região da vibrissa (ELISA) foi feita após o teste da formalina e análise da atividade locomotora dos animais por meio do teste da coordenação motora. Resultados: no teste de quantificação do α-felandreno por CLAE foi encontrado um tempo de retenção de 5,8 minutos e um pico de boa resolução cromatográfica, o coeficiente de correlação R 2 = 0,9998 comprova a linearidade do método e os resultados do limite de detecção (LD) e quantificação (LQ) ( 0,81 e 2,44 µg/mL, respectivamente) demonstraram a sensibilidade do método. Os resultados do DSC indicaram uma maior estabilidade do α-felandreno quando incorporado a HPβ-CD. Na nocicepção induzida por formalina observou-se efeito antinociceptivo significativo nos animais tratados com FEN e FEN-HPβCD 50mg/kg quando comparados com o veículo tanto na fase neurogênica (p<0,001 para ambos) quanto inflamatória (p<0,001 e p<0,05, respectivamente), paralelo à isso, observou-se uma inibição das citocinas TNF-α (p<0,05 e p<0,01, respectivamente) e IL-1β (p<0,01 e p<0,05, respectivamente). O tratamento com FEN e FEN-HPβCD 50mg/kg quando comparados com o veículo reduziram significativamente a nocicepção induzida por cinamaldeído (p<0,01 e p<0,001, respectivamente) , glutamato (p<0,001 e p<0,01, respectivamente) e salina hipertônica (p<0,05). No teste de coordenação motora feito por meio do aparelho rota-rod, não foram observados alteração da atividade locomotora dos roedores, após tratamento com FEN e FENHPβCD 50mg/kg. Conclusão: pode-se concluir então que o monoterpeno α-felandreno possui efeito antinociceptivo nos modelo testados, provavelmente envolvendo a ativação dos canais TRPV1, TRPA1, receptores glutamatérgicos e liberação das citocinas TNF-α e IL-1β. Mais estudos deverão ser realizados para elucidar o mecanismo de ação a nível central e periférico do felandreno, assim como sua ação em outros modelos de dor para que seja comprovado seu efeito analgésico.São CristóvãoporCiências farmacêuticasMonoterpenosDor nociceptivaMatéria médica vegetalDor orofacialNocicepção orofacialFitoterapiaMonoterpenosα-felandrenoOrofacial nociceptionPhytotherapyMonoterpenesα-phellandreneCIENCIAS BIOLOGICAS::FARMACOLOGIAEfeito do α-felandreno complexado e não complexado em hidroxipropil-β-ciclodextrina na nocicepção orofacial em roedoresEffect of complexed and uncomplexed α-phelandrene in Hydroxypropyl-β-Cyclodextrin on orofacial nociception in rodentsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisPós-Graduação em Ciências FarmacêuticasUniversidade Federal de Sergipereponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessORIGINALBRENNDA_GONZAGA_MACHADO.pdfBRENNDA_GONZAGA_MACHADO.pdfapplication/pdf2288805https://ri.ufs.br/jspui/bitstream/riufs/16820/2/BRENNDA_GONZAGA_MACHADO.pdf55ce5ac09f53ea8f31b499210163f498MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/16820/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51TEXTBRENNDA_GONZAGA_MACHADO.pdf.txtBRENNDA_GONZAGA_MACHADO.pdf.txtExtracted texttext/plain86619https://ri.ufs.br/jspui/bitstream/riufs/16820/3/BRENNDA_GONZAGA_MACHADO.pdf.txtc2cd3d212c6139f7b82d60540efc3b7fMD53THUMBNAILBRENNDA_GONZAGA_MACHADO.pdf.jpgBRENNDA_GONZAGA_MACHADO.pdf.jpgGenerated Thumbnailimage/jpeg1780https://ri.ufs.br/jspui/bitstream/riufs/16820/4/BRENNDA_GONZAGA_MACHADO.pdf.jpgdfbdb8ddafa39c27b65966b9ad21e13bMD54riufs/168202022-11-24 11:36:30.581oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2022-11-24T14:36:30Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.pt_BR.fl_str_mv |
Efeito do α-felandreno complexado e não complexado em hidroxipropil-β-ciclodextrina na nocicepção orofacial em roedores |
dc.title.alternative.eng.fl_str_mv |
Effect of complexed and uncomplexed α-phelandrene in Hydroxypropyl-β-Cyclodextrin on orofacial nociception in rodents |
title |
Efeito do α-felandreno complexado e não complexado em hidroxipropil-β-ciclodextrina na nocicepção orofacial em roedores |
spellingShingle |
Efeito do α-felandreno complexado e não complexado em hidroxipropil-β-ciclodextrina na nocicepção orofacial em roedores Machado, Brennda Gonzaga Ciências farmacêuticas Monoterpenos Dor nociceptiva Matéria médica vegetal Dor orofacial Nocicepção orofacial Fitoterapia Monoterpenos α-felandreno Orofacial nociception Phytotherapy Monoterpenes α-phellandrene CIENCIAS BIOLOGICAS::FARMACOLOGIA |
title_short |
Efeito do α-felandreno complexado e não complexado em hidroxipropil-β-ciclodextrina na nocicepção orofacial em roedores |
title_full |
Efeito do α-felandreno complexado e não complexado em hidroxipropil-β-ciclodextrina na nocicepção orofacial em roedores |
title_fullStr |
Efeito do α-felandreno complexado e não complexado em hidroxipropil-β-ciclodextrina na nocicepção orofacial em roedores |
title_full_unstemmed |
Efeito do α-felandreno complexado e não complexado em hidroxipropil-β-ciclodextrina na nocicepção orofacial em roedores |
title_sort |
Efeito do α-felandreno complexado e não complexado em hidroxipropil-β-ciclodextrina na nocicepção orofacial em roedores |
author |
Machado, Brennda Gonzaga |
author_facet |
Machado, Brennda Gonzaga |
author_role |
author |
dc.contributor.author.fl_str_mv |
Machado, Brennda Gonzaga |
dc.contributor.advisor1.fl_str_mv |
Antoniolli, Ângelo Roberto |
dc.contributor.advisor-co1.fl_str_mv |
Quintans, Jullyana de Souza Siqueira |
contributor_str_mv |
Antoniolli, Ângelo Roberto Quintans, Jullyana de Souza Siqueira |
dc.subject.por.fl_str_mv |
Ciências farmacêuticas Monoterpenos Dor nociceptiva Matéria médica vegetal Dor orofacial Nocicepção orofacial Fitoterapia Monoterpenos α-felandreno |
topic |
Ciências farmacêuticas Monoterpenos Dor nociceptiva Matéria médica vegetal Dor orofacial Nocicepção orofacial Fitoterapia Monoterpenos α-felandreno Orofacial nociception Phytotherapy Monoterpenes α-phellandrene CIENCIAS BIOLOGICAS::FARMACOLOGIA |
dc.subject.eng.fl_str_mv |
Orofacial nociception Phytotherapy Monoterpenes α-phellandrene |
dc.subject.cnpq.fl_str_mv |
CIENCIAS BIOLOGICAS::FARMACOLOGIA |
description |
Objective: the work aims to expand the studies involving the pharmacological effects of the αphelandrene monoterpene, specifically in the promotion of orofacial antinociception. Methods: a hydroxypropil-β-cyclodextrin (HPβ-CD) complex was prepared with incorporation of the α-felandorene that was later physically characterized through high efficiency liquid chromatography (CLAE) and differential exploratory calorimetry (DSC). Seeking to evaluate the possible analgesic action of α-felandreno in animal models in orofacial nociception, the animals were divided into four groups (n = 8 per group) and treated with unlapled α-felandreno (FEN) at the 50mg/kg oral dose , α-felandreno complex in hydroxipropil-β-cyclodextrin (FENHPβCD) at a dose of 50mg/kg oral via, negative control (tween 80 0.2% in water) 10ml/kg per gavage and positive controls (morphine 10mg/kg i.p. For tests of formaline, glutamate and hypertonic saline; camphor 7.6mg/kg i.p. for the scine test and, diazepam 3mg/kg i.p. for the rod rod test). Finally, quantification of TNF-α and IL-1β cytokines was made in the Vibrissa region after the formaline test and animal locomotor analysis analysis through the motor coordination test and data collection for statistical evaluations. Results: in the α-felandorereno quantification test by Clae, a 5.8 minutes retention time was found and a peak of good chromatographic resolution, the correlation coefficient R2 = 0.9998 proves the linearity of the method and the limit results Detection (LD) and quantification (LQ) (0.81 and 2.44 µg/mL, respectively) demonstrated the sensitivity of the method. DSC results indicated greater α-felandreno stability when incorporated into HPβ-CD. In formalin-induced nociception, a significant antinociceptive effect was observed in animals treated with FEN and FEN-HPβCD 50mg/kg when compared with the vehicle in both the neurogenic (p<0.001 for both) and inflammatory (p<0.001 and p<005, respectively) phases, parallel to this, there was an inhibition of the cytokines TNF-α (p<0.05 and p<0.01, respectively) and IL-1β (p<0.01 and p<0.05, respectively). The treatment with FEN and FEN-HPβCD 50mg/kg when compared to the vehicle significantly reduced the nociception induced by cinnamaldehyde (p<0.01 and p<0.001, respectively), glutamate (p<0.001 and p<0.01, respectively) and hypertonic saline (p<0.05). In the motor coordination test done through the Rota-Rod apparatus, no behaviors were observed that influenced the locomotor activity of rodents, as animals treated with Fen and FEN-HPβCD 50mg/kg remained in the bar for more than 170 seconds in Every time (30, 60 and 120 minutes). Conclusion: it can then be concluded that α-felandorene monoterpene has antinociceptive effect, probably involving TRPA1, TRPA1, glutamatergic receptors and release of TNF-α and IL-1β cytokines. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-11-24T14:34:23Z |
dc.date.available.fl_str_mv |
2022-11-24T14:34:23Z |
dc.date.issued.fl_str_mv |
2022-08-24 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MACHADO, Brennda Gonzaga. Efeito do α-felandreno complexado e não complexado em hidroxipropil-β-ciclodextrina na nocicepção orofacial em roedores. 2022. 58 f. Dissertação (Mestrado em Ciências Farmacêuticas) – Universidade Federal de Sergipe, São Cristóvão, 2022. |
dc.identifier.uri.fl_str_mv |
http://ri.ufs.br/jspui/handle/riufs/16820 |
identifier_str_mv |
MACHADO, Brennda Gonzaga. Efeito do α-felandreno complexado e não complexado em hidroxipropil-β-ciclodextrina na nocicepção orofacial em roedores. 2022. 58 f. Dissertação (Mestrado em Ciências Farmacêuticas) – Universidade Federal de Sergipe, São Cristóvão, 2022. |
url |
http://ri.ufs.br/jspui/handle/riufs/16820 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.program.fl_str_mv |
Pós-Graduação em Ciências Farmacêuticas |
dc.publisher.initials.fl_str_mv |
Universidade Federal de Sergipe |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFS instname:Universidade Federal de Sergipe (UFS) instacron:UFS |
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Universidade Federal de Sergipe (UFS) |
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UFS |
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UFS |
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