Development of DNA probe for detection of Aujeszky's disease virus
Autor(a) principal: | |
---|---|
Data de Publicação: | 1999 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFS |
Texto Completo: | https://ri.ufs.br/handle/riufs/864 |
Resumo: | Uma sonda radioativa e uma outra biotinilada foram produzidas para detecção do vírus da doença de Aujeszky (VDA). O fragmento de Bam H I 7 do DNA genômico do VDA foi marcado por "nick translation" empregando P32dCTP e um fragmento de 196pb, resultante de uma amplificação pela reação em cadeia pela polimerase marcado com biotina 7-dATP. Essas sondas, de uma maneira rápida e específica, prestaram-se para a detecção da infecção aguda pelo VDA. Entretanto, não se mostraram sensíveis o suficiente para detectar seqüências genômicas de VDA no gânglio trigêmio de suínos e camundongos com infecção latente. _________________________________________________________________________________________ ABSTRACT: A DNA hybridization dot-blot assay using a radioactive and a non-radioactive probe has been developed for the detection of Aujeszky's disease virus (ADV). The Bam H I 7 fragment of ADV genomic DNA was labeled by nick translation using 32P-dCTP and the 196bp polymerase chain reaction (PCR) gG glycoprotein gene amplified fragment was also labeled by nick translation but using biotin d-7-dATP. This technique provides a fast and effective means of detecting acute cases of ADV infection but it was unable to detect ADV nucleic acid sequences in trigeminal nerve ganglia of latent infected pigs and mice. |
id |
UFS-2_aaecc273acfa61d02a27a8b457c3a297 |
---|---|
oai_identifier_str |
oai:ufs.br:riufs/864 |
network_acronym_str |
UFS-2 |
network_name_str |
Repositório Institucional da UFS |
repository_id_str |
|
spelling |
Cândido, Alexandre LunaBarros, Patrícia Pimental deResende, MaurícioFlatschart, Roberto Becht2014-02-03T19:00:53Z2014-02-03T19:00:53Z1999-08CANDIDO, A. L. et al. Development of DNA probe for detection of Aujeszky's disease virus. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 51, n. 4, Ago. 1999. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000400001&lng=en&nrm=iso>. Acesso em: 3 fev. 2014.0102-0935https://ri.ufs.br/handle/riufs/864Creative Commons Attribution LicenseUma sonda radioativa e uma outra biotinilada foram produzidas para detecção do vírus da doença de Aujeszky (VDA). O fragmento de Bam H I 7 do DNA genômico do VDA foi marcado por "nick translation" empregando P32dCTP e um fragmento de 196pb, resultante de uma amplificação pela reação em cadeia pela polimerase marcado com biotina 7-dATP. Essas sondas, de uma maneira rápida e específica, prestaram-se para a detecção da infecção aguda pelo VDA. Entretanto, não se mostraram sensíveis o suficiente para detectar seqüências genômicas de VDA no gânglio trigêmio de suínos e camundongos com infecção latente. _________________________________________________________________________________________ ABSTRACT: A DNA hybridization dot-blot assay using a radioactive and a non-radioactive probe has been developed for the detection of Aujeszky's disease virus (ADV). The Bam H I 7 fragment of ADV genomic DNA was labeled by nick translation using 32P-dCTP and the 196bp polymerase chain reaction (PCR) gG glycoprotein gene amplified fragment was also labeled by nick translation but using biotin d-7-dATP. This technique provides a fast and effective means of detecting acute cases of ADV infection but it was unable to detect ADV nucleic acid sequences in trigeminal nerve ganglia of latent infected pigs and mice.Escola de Veterinária UFMGDoença de AujeszkyHibridizaçãoDNASonda radioativaSonda não radioativaDevelopment of DNA probe for detection of Aujeszky's disease virusDesenvolvimento de uma sonda de DNA para a detecção do vírus da doença de Aujeszkyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessTHUMBNAILDevelopmentDNAAujeszky.pdf.jpgDevelopmentDNAAujeszky.pdf.jpgGenerated Thumbnailimage/jpeg1767https://ri.ufs.br/jspui/bitstream/riufs/864/4/DevelopmentDNAAujeszky.pdf.jpge6f5a9aa5a515010fc14b629bb44a401MD54ORIGINALDevelopmentDNAAujeszky.pdfDevelopmentDNAAujeszky.pdfapplication/pdf216727https://ri.ufs.br/jspui/bitstream/riufs/864/1/DevelopmentDNAAujeszky.pdfbcf1ee9b53115db65833c2c7c3e4938cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://ri.ufs.br/jspui/bitstream/riufs/864/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTDevelopmentDNAAujeszky.pdf.txtDevelopmentDNAAujeszky.pdf.txtExtracted texttext/plain17642https://ri.ufs.br/jspui/bitstream/riufs/864/3/DevelopmentDNAAujeszky.pdf.txt51ff9e82c7d0f2c0c546f08478cddc1aMD53riufs/8642014-08-15 20:17:43.508oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2014-08-15T23:17:43Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false |
dc.title.pt_BR.fl_str_mv |
Development of DNA probe for detection of Aujeszky's disease virus |
dc.title.alternative.pt_BR.fl_str_mv |
Desenvolvimento de uma sonda de DNA para a detecção do vírus da doença de Aujeszky |
title |
Development of DNA probe for detection of Aujeszky's disease virus |
spellingShingle |
Development of DNA probe for detection of Aujeszky's disease virus Cândido, Alexandre Luna Doença de Aujeszky Hibridização DNA Sonda radioativa Sonda não radioativa |
title_short |
Development of DNA probe for detection of Aujeszky's disease virus |
title_full |
Development of DNA probe for detection of Aujeszky's disease virus |
title_fullStr |
Development of DNA probe for detection of Aujeszky's disease virus |
title_full_unstemmed |
Development of DNA probe for detection of Aujeszky's disease virus |
title_sort |
Development of DNA probe for detection of Aujeszky's disease virus |
author |
Cândido, Alexandre Luna |
author_facet |
Cândido, Alexandre Luna Barros, Patrícia Pimental de Resende, Maurício Flatschart, Roberto Becht |
author_role |
author |
author2 |
Barros, Patrícia Pimental de Resende, Maurício Flatschart, Roberto Becht |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Cândido, Alexandre Luna Barros, Patrícia Pimental de Resende, Maurício Flatschart, Roberto Becht |
dc.subject.por.fl_str_mv |
Doença de Aujeszky Hibridização DNA Sonda radioativa Sonda não radioativa |
topic |
Doença de Aujeszky Hibridização DNA Sonda radioativa Sonda não radioativa |
description |
Uma sonda radioativa e uma outra biotinilada foram produzidas para detecção do vírus da doença de Aujeszky (VDA). O fragmento de Bam H I 7 do DNA genômico do VDA foi marcado por "nick translation" empregando P32dCTP e um fragmento de 196pb, resultante de uma amplificação pela reação em cadeia pela polimerase marcado com biotina 7-dATP. Essas sondas, de uma maneira rápida e específica, prestaram-se para a detecção da infecção aguda pelo VDA. Entretanto, não se mostraram sensíveis o suficiente para detectar seqüências genômicas de VDA no gânglio trigêmio de suínos e camundongos com infecção latente. _________________________________________________________________________________________ ABSTRACT: A DNA hybridization dot-blot assay using a radioactive and a non-radioactive probe has been developed for the detection of Aujeszky's disease virus (ADV). The Bam H I 7 fragment of ADV genomic DNA was labeled by nick translation using 32P-dCTP and the 196bp polymerase chain reaction (PCR) gG glycoprotein gene amplified fragment was also labeled by nick translation but using biotin d-7-dATP. This technique provides a fast and effective means of detecting acute cases of ADV infection but it was unable to detect ADV nucleic acid sequences in trigeminal nerve ganglia of latent infected pigs and mice. |
publishDate |
1999 |
dc.date.issued.fl_str_mv |
1999-08 |
dc.date.accessioned.fl_str_mv |
2014-02-03T19:00:53Z |
dc.date.available.fl_str_mv |
2014-02-03T19:00:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CANDIDO, A. L. et al. Development of DNA probe for detection of Aujeszky's disease virus. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 51, n. 4, Ago. 1999. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000400001&lng=en&nrm=iso>. Acesso em: 3 fev. 2014. |
dc.identifier.uri.fl_str_mv |
https://ri.ufs.br/handle/riufs/864 |
dc.identifier.issn.none.fl_str_mv |
0102-0935 |
dc.identifier.license.pt_BR.fl_str_mv |
Creative Commons Attribution License |
identifier_str_mv |
CANDIDO, A. L. et al. Development of DNA probe for detection of Aujeszky's disease virus. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 51, n. 4, Ago. 1999. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000400001&lng=en&nrm=iso>. Acesso em: 3 fev. 2014. 0102-0935 Creative Commons Attribution License |
url |
https://ri.ufs.br/handle/riufs/864 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Escola de Veterinária UFMG |
publisher.none.fl_str_mv |
Escola de Veterinária UFMG |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFS instname:Universidade Federal de Sergipe (UFS) instacron:UFS |
instname_str |
Universidade Federal de Sergipe (UFS) |
instacron_str |
UFS |
institution |
UFS |
reponame_str |
Repositório Institucional da UFS |
collection |
Repositório Institucional da UFS |
bitstream.url.fl_str_mv |
https://ri.ufs.br/jspui/bitstream/riufs/864/4/DevelopmentDNAAujeszky.pdf.jpg https://ri.ufs.br/jspui/bitstream/riufs/864/1/DevelopmentDNAAujeszky.pdf https://ri.ufs.br/jspui/bitstream/riufs/864/2/license.txt https://ri.ufs.br/jspui/bitstream/riufs/864/3/DevelopmentDNAAujeszky.pdf.txt |
bitstream.checksum.fl_str_mv |
e6f5a9aa5a515010fc14b629bb44a401 bcf1ee9b53115db65833c2c7c3e4938c 8a4605be74aa9ea9d79846c1fba20a33 51ff9e82c7d0f2c0c546f08478cddc1a |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS) |
repository.mail.fl_str_mv |
repositorio@academico.ufs.br |
_version_ |
1802110668178980864 |