Molecular detection of toxigenic potential of fungi in peanut samples collected in retail shops in Maringá/PR, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Biotemas (Online) |
Texto Completo: | https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n1p13 |
Resumo: | Many foods are susceptible to fungal contamination. Grains, such as peanuts, are commonly affected, with consequences including compromised integrity and infeasibility for human and animal consumption. Furthermore, some fungi may pose a health risk, largely due the production of mycotoxins. Among these, alatoxins produced by Aspergillus lavus and A. parasiticus produce various carcinogenic, teratogenic, immunosuppressive, hepatotoxic and nephrotoxic effects. Molecular techniques have been used to identify and distinguish fungal species in foods. The objective of this study was molecular detection of Aspergillus species in peanut samples collected in stores in Maringá-PR, by amplification of fungal genetic material with specific primers for the intergenic spacer alR-alJ and later cutting with restriction enzymes. Of the 50 peanut samples analyzed, 27 were positive for the intergenic spacer alR-alJ, seven of which were identified as Aspergillus flavus. Our results demonstrate that peanuts sold in retail stores in this region have potential for contamination with toxigenic fungi. |
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Molecular detection of toxigenic potential of fungi in peanut samples collected in retail shops in Maringá/PR, BrazilDetecção molecular de fungos com potencial toxigênico em amostras de amendoim vendidas no comércio varejista de Maringá/PR, BrasilMany foods are susceptible to fungal contamination. Grains, such as peanuts, are commonly affected, with consequences including compromised integrity and infeasibility for human and animal consumption. Furthermore, some fungi may pose a health risk, largely due the production of mycotoxins. Among these, alatoxins produced by Aspergillus lavus and A. parasiticus produce various carcinogenic, teratogenic, immunosuppressive, hepatotoxic and nephrotoxic effects. Molecular techniques have been used to identify and distinguish fungal species in foods. The objective of this study was molecular detection of Aspergillus species in peanut samples collected in stores in Maringá-PR, by amplification of fungal genetic material with specific primers for the intergenic spacer alR-alJ and later cutting with restriction enzymes. Of the 50 peanut samples analyzed, 27 were positive for the intergenic spacer alR-alJ, seven of which were identified as Aspergillus flavus. Our results demonstrate that peanuts sold in retail stores in this region have potential for contamination with toxigenic fungi.Muitos alimentos são susceptíveis a contaminação por fungos. Os grãos, como o amendoim, são largamente acometidos, tendo como consequências o comprometimento de sua integridade e inviabilidade para o consumo humano e animal. Além disso, representam riscos à saúde, principalmente pela produção de micotoxinas. Dentre estas, as alatoxinas, produzidas principalmente por Aspergillus lavus e A. parasiticus, produzem diversos efeitos tóxicos carcinogênicos, teratogênicos, imunossupressores, hepatóxicos e nefrotóxicos. Técnicas moleculares têm sido utilizadas para identiicação e discriminação de espécies de fungos em alimentos. O objetivo deste trabalho foi realizar a detecção molecular de espécies de Aspergillus em amostras de amendoim coletadas no comércio varejista de Maringá-PR, através da ampliicação do material genético fúngico com primers especíicos para o espaçador intergênico alR-alJ e posterior corte com enzimas de restrição. Das 50 amostras de amendoim analisadas, 27 foram positivas em relação à presença do espaçador intergênico alR-alJ, sendo que destas sete foram identiicadas como Aspergillus lavus. Dessa forma, veriica-se a presença de amostras de amendoim contaminadas com fungos com potencial toxigênico no comércio varejista de Maringá/PR. Universidade Federal de Santa Catarina2015-01-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n1p1310.5007/2175-7925.2015v28n1p13Biotemas; v. 28 n. 1 (2015); 13-192175-79250103-1643reponame:Biotemas (Online)instname:Universidade Federal de Santa Catarina (UFSC)instacron:UFSCporhttps://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n1p13/28658Copyright (c) 2015 Alessandra Valéria Oliveira, Cíntia Corteccioni Nuñez Del Prado, Nathan Gomes Modesto, Gislaine de Lucenainfo:eu-repo/semantics/openAccessOliveira, Alessandra ValériaNuñez Del Prado, Cíntia CorteccioniModesto, Nathan GomesLucena, Gislaine de2017-10-19T16:47:19Zoai:periodicos.ufsc.br:article/32509Revistahttp://www.biotemas.ufsc.br/index.htmPUBhttps://periodicos.ufsc.br/index.php/biotemas/oai||carlospinto@ccb.ufsc.br2175-79250103-1643opendoar:2017-10-19T16:47:19Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC)false |
dc.title.none.fl_str_mv |
Molecular detection of toxigenic potential of fungi in peanut samples collected in retail shops in Maringá/PR, Brazil Detecção molecular de fungos com potencial toxigênico em amostras de amendoim vendidas no comércio varejista de Maringá/PR, Brasil |
title |
Molecular detection of toxigenic potential of fungi in peanut samples collected in retail shops in Maringá/PR, Brazil |
spellingShingle |
Molecular detection of toxigenic potential of fungi in peanut samples collected in retail shops in Maringá/PR, Brazil Oliveira, Alessandra Valéria |
title_short |
Molecular detection of toxigenic potential of fungi in peanut samples collected in retail shops in Maringá/PR, Brazil |
title_full |
Molecular detection of toxigenic potential of fungi in peanut samples collected in retail shops in Maringá/PR, Brazil |
title_fullStr |
Molecular detection of toxigenic potential of fungi in peanut samples collected in retail shops in Maringá/PR, Brazil |
title_full_unstemmed |
Molecular detection of toxigenic potential of fungi in peanut samples collected in retail shops in Maringá/PR, Brazil |
title_sort |
Molecular detection of toxigenic potential of fungi in peanut samples collected in retail shops in Maringá/PR, Brazil |
author |
Oliveira, Alessandra Valéria |
author_facet |
Oliveira, Alessandra Valéria Nuñez Del Prado, Cíntia Corteccioni Modesto, Nathan Gomes Lucena, Gislaine de |
author_role |
author |
author2 |
Nuñez Del Prado, Cíntia Corteccioni Modesto, Nathan Gomes Lucena, Gislaine de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Alessandra Valéria Nuñez Del Prado, Cíntia Corteccioni Modesto, Nathan Gomes Lucena, Gislaine de |
description |
Many foods are susceptible to fungal contamination. Grains, such as peanuts, are commonly affected, with consequences including compromised integrity and infeasibility for human and animal consumption. Furthermore, some fungi may pose a health risk, largely due the production of mycotoxins. Among these, alatoxins produced by Aspergillus lavus and A. parasiticus produce various carcinogenic, teratogenic, immunosuppressive, hepatotoxic and nephrotoxic effects. Molecular techniques have been used to identify and distinguish fungal species in foods. The objective of this study was molecular detection of Aspergillus species in peanut samples collected in stores in Maringá-PR, by amplification of fungal genetic material with specific primers for the intergenic spacer alR-alJ and later cutting with restriction enzymes. Of the 50 peanut samples analyzed, 27 were positive for the intergenic spacer alR-alJ, seven of which were identified as Aspergillus flavus. Our results demonstrate that peanuts sold in retail stores in this region have potential for contamination with toxigenic fungi. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-07 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n1p13 10.5007/2175-7925.2015v28n1p13 |
url |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n1p13 |
identifier_str_mv |
10.5007/2175-7925.2015v28n1p13 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n1p13/28658 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
dc.source.none.fl_str_mv |
Biotemas; v. 28 n. 1 (2015); 13-19 2175-7925 0103-1643 reponame:Biotemas (Online) instname:Universidade Federal de Santa Catarina (UFSC) instacron:UFSC |
instname_str |
Universidade Federal de Santa Catarina (UFSC) |
instacron_str |
UFSC |
institution |
UFSC |
reponame_str |
Biotemas (Online) |
collection |
Biotemas (Online) |
repository.name.fl_str_mv |
Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC) |
repository.mail.fl_str_mv |
||carlospinto@ccb.ufsc.br |
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1799940553425551360 |