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Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Biotemas (Online) |
Texto Completo: | https://periodicos.ufsc.br/index.php/biotemas/article/view/21410 |
Resumo: | Extracted DNA from basidiomata of frozen field-collected and herbarium specimens collected in different periods and maintained in a variety of ways was evaluated according to general condition, color, spectral absorbance ratio, final concentration and PCR amplification, using two distinct and most common extraction methods: SDS (Sodium Doecyl Sulphate) and CTAB (Cetyl Trimetyl Ammonium Bromide). Most of the frozen fieldcollected basidiomata presented a very good to good DNA condition and no degradation. All the herbarium specimens exhibited poor DNA condition with low molecular weight bands. Practically all the DNA solutions of the samples were colored, and spectral absorbance ratios were considerably variable, with lower values observed in darker ones. All the samples were successfully PCR-amplified. DNA extraction of Herbarium specimens and also specimens with high polysaccharide content was only achievable using the CTAB method. For an all-purpose DNA extraction from field-collected and dehydrated coriaceous and woody basidiomata bearing a high content of secondary metabolites and/or polysaccharides, the CTAB-based method is definitely best the choice of method. |
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.DNA extraction from frozen fieldcollected and dehydrated herbarium fungal basidiomata: performance of SDS and CTAB-based methodsExtracted DNA from basidiomata of frozen field-collected and herbarium specimens collected in different periods and maintained in a variety of ways was evaluated according to general condition, color, spectral absorbance ratio, final concentration and PCR amplification, using two distinct and most common extraction methods: SDS (Sodium Doecyl Sulphate) and CTAB (Cetyl Trimetyl Ammonium Bromide). Most of the frozen fieldcollected basidiomata presented a very good to good DNA condition and no degradation. All the herbarium specimens exhibited poor DNA condition with low molecular weight bands. Practically all the DNA solutions of the samples were colored, and spectral absorbance ratios were considerably variable, with lower values observed in darker ones. All the samples were successfully PCR-amplified. DNA extraction of Herbarium specimens and also specimens with high polysaccharide content was only achievable using the CTAB method. For an all-purpose DNA extraction from field-collected and dehydrated coriaceous and woody basidiomata bearing a high content of secondary metabolites and/or polysaccharides, the CTAB-based method is definitely best the choice of method.Extração de DNA de basidiomas congelados e de Herbário, através dos métodos SDS e CTAB. A extração deDNA de basidomas coletados e congelados e de espécimes de herbário coletados em diferentes períodos e mantidos de diferentes formas foi avaliada de acordo a condição geral, cor, razão de absorvância espectral, concentração final e amplificação PCR, utilizando dois dos métodos mais comuns de extração SDS (Sodium Doecyl Sulphate) e CTAB (Cetyl Trimetyl Ammonium Bromide). A maioria dos basidiomas congelados apresentou uma condição de DNA boa a muito boa e sem degradação. Todos os espécimes de herbário exibiram DNA em má condição com bandas de baixo peso molecular. Praticamente todas soluções de DNA das amostras foram coloridas e as taxas de absorbância foram consideravelmente variáveis, com valores menores nas zonas escuras. Todas as amostras foram amplificadas em PCR com sucesso. A extração de DNA de espécimes com alto conteúdo de polisacarídeos e de espécimes de herbário foram obtidas unicamente pelo método CTAB. Para uma proposta geral de extração de DNA de basidiomas coriáceos e lenhosos recém coletados e desidratados, com alto conteúdo de metabólitos secundários e/ou polissacarídeos, o método CTAB é a melhor escolha.Universidade Federal de Santa Catarina2005-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsc.br/index.php/biotemas/article/view/21410Biotemas; v. 18 n. 2 (2005); 19-322175-79250103-1643reponame:Biotemas (Online)instname:Universidade Federal de Santa Catarina (UFSC)instacron:UFSCporhttps://periodicos.ufsc.br/index.php/biotemas/article/view/21410/19377Copyright (c) 2005 Aristóteles Góes-Neto, Clarice Loguercio-Leite, Rosa Trinidad Guerrerohttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessGóes-Neto, AristótelesLoguercio-Leite, ClariceGuerrero, Rosa Trinidad2022-11-21T11:28:32Zoai:periodicos.ufsc.br:article/21410Revistahttp://www.biotemas.ufsc.br/index.htmPUBhttps://periodicos.ufsc.br/index.php/biotemas/oai||carlospinto@ccb.ufsc.br2175-79250103-1643opendoar:2022-11-21T11:28:32Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC)false |
dc.title.none.fl_str_mv |
. DNA extraction from frozen fieldcollected and dehydrated herbarium fungal basidiomata: performance of SDS and CTAB-based methods |
title |
. |
spellingShingle |
. Góes-Neto, Aristóteles |
title_short |
. |
title_full |
. |
title_fullStr |
. |
title_full_unstemmed |
. |
title_sort |
. |
author |
Góes-Neto, Aristóteles |
author_facet |
Góes-Neto, Aristóteles Loguercio-Leite, Clarice Guerrero, Rosa Trinidad |
author_role |
author |
author2 |
Loguercio-Leite, Clarice Guerrero, Rosa Trinidad |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Góes-Neto, Aristóteles Loguercio-Leite, Clarice Guerrero, Rosa Trinidad |
description |
Extracted DNA from basidiomata of frozen field-collected and herbarium specimens collected in different periods and maintained in a variety of ways was evaluated according to general condition, color, spectral absorbance ratio, final concentration and PCR amplification, using two distinct and most common extraction methods: SDS (Sodium Doecyl Sulphate) and CTAB (Cetyl Trimetyl Ammonium Bromide). Most of the frozen fieldcollected basidiomata presented a very good to good DNA condition and no degradation. All the herbarium specimens exhibited poor DNA condition with low molecular weight bands. Practically all the DNA solutions of the samples were colored, and spectral absorbance ratios were considerably variable, with lower values observed in darker ones. All the samples were successfully PCR-amplified. DNA extraction of Herbarium specimens and also specimens with high polysaccharide content was only achievable using the CTAB method. For an all-purpose DNA extraction from field-collected and dehydrated coriaceous and woody basidiomata bearing a high content of secondary metabolites and/or polysaccharides, the CTAB-based method is definitely best the choice of method. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/21410 |
url |
https://periodicos.ufsc.br/index.php/biotemas/article/view/21410 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/21410/19377 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2005 Aristóteles Góes-Neto, Clarice Loguercio-Leite, Rosa Trinidad Guerrero http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2005 Aristóteles Góes-Neto, Clarice Loguercio-Leite, Rosa Trinidad Guerrero http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
dc.source.none.fl_str_mv |
Biotemas; v. 18 n. 2 (2005); 19-32 2175-7925 0103-1643 reponame:Biotemas (Online) instname:Universidade Federal de Santa Catarina (UFSC) instacron:UFSC |
instname_str |
Universidade Federal de Santa Catarina (UFSC) |
instacron_str |
UFSC |
institution |
UFSC |
reponame_str |
Biotemas (Online) |
collection |
Biotemas (Online) |
repository.name.fl_str_mv |
Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC) |
repository.mail.fl_str_mv |
||carlospinto@ccb.ufsc.br |
_version_ |
1799940550941474816 |