Proteção do óxido nítrico sobre o estresse oxidativo causado por herbicidas em arroz irrigado
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/3254 |
Resumo: | Although selective, some herbicides cause injury in plants, resulting in uncontrolled production of reactive oxygen species. Nitric oxide (NO) is appointed as a reducer of the effects caused by oxidative stress in plants; however, their mitigation mechanisms are not yet clear. This study aimed to evaluate the nitric oxide protection mechanisms, provided by sodium nitroprusside (SNP), in the flooded rice cultivar Puitá INTA CL exposed to clomazone herbicide (360 g a.i. L-1), oxyfluorfen (240 g a.i. L-1), oxadiazon (250 g a.i. L-1) and the formulated mixture of paraquat (200 g a.i. L-1) + diuron (100 g a.i. L-1) in vitro and in vivo. For this purpose, (i) leaf segments were pre-incubated with 0, 200 and 2000 μM of SNP and exposed to five herbicides concentrations, and the protective effect confirmed by the NO scavenger, carboxyPTIO, (ii) seeds were soaked by one and 10 hours in solution containing 200 μM of SNP and after sowing, clomazone (1.7 and 3.4 L ha-1 of c.p.) was applied in the post-seeding and the needle stage (iii) plants were treated with 200 and 2000 μM of SNP and sprayed with 1.0 L ha-1 of herbicides oxadiazon, oxyfluorfen + diuron and paraquat. The concentration of 200 μM of SNP has reduced, up to 14% the electrolyte leakage in rice leaf segments exposed to 755.9 μM of oxadiazon, as well as, the imbibition of seeds with 200 μM of SNP for 10 hours increased by 33.1% the total content of chlorophyll, maintaining the pigmentation of seedlings exposed to 3.4 L h-1 of clomazone, applied to the needle stage. When sprayed on the shoot, paraquat + diuron, oxyfluorfen and oxadiazon, respectively, caused the ions leakage of 89.8; 62.8 and 18.3%, while the pre-treatment of the plants with 2000 μM of SNP decreased by 11 and 25.4% the damage caused by the first two, however, there was not reduction in injury caused by oxadiazon. The pre-treatment with SNP minimized the oxidative stress caused by herbicides, by the reducing in the hydrogen peroxide levels and TBARS and by the increase in the pigments levels, reflecting on the lower activity of the enzymes SOD and POD, however, such changes are dependent on the SNP concentration, exposure time and the level of stress caused by herbicides. In the absence or low levels of stress, NO was toxic to plants. |
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2017-05-102017-05-102015-07-31SILVA, Keli Souza da. Protection of nitric oxide on the oxidative stress caused by herbicides in the flooded rice. 2015. 87 f. Tese (Doutorado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2015.http://repositorio.ufsm.br/handle/1/3254Although selective, some herbicides cause injury in plants, resulting in uncontrolled production of reactive oxygen species. Nitric oxide (NO) is appointed as a reducer of the effects caused by oxidative stress in plants; however, their mitigation mechanisms are not yet clear. This study aimed to evaluate the nitric oxide protection mechanisms, provided by sodium nitroprusside (SNP), in the flooded rice cultivar Puitá INTA CL exposed to clomazone herbicide (360 g a.i. L-1), oxyfluorfen (240 g a.i. L-1), oxadiazon (250 g a.i. L-1) and the formulated mixture of paraquat (200 g a.i. L-1) + diuron (100 g a.i. L-1) in vitro and in vivo. For this purpose, (i) leaf segments were pre-incubated with 0, 200 and 2000 μM of SNP and exposed to five herbicides concentrations, and the protective effect confirmed by the NO scavenger, carboxyPTIO, (ii) seeds were soaked by one and 10 hours in solution containing 200 μM of SNP and after sowing, clomazone (1.7 and 3.4 L ha-1 of c.p.) was applied in the post-seeding and the needle stage (iii) plants were treated with 200 and 2000 μM of SNP and sprayed with 1.0 L ha-1 of herbicides oxadiazon, oxyfluorfen + diuron and paraquat. The concentration of 200 μM of SNP has reduced, up to 14% the electrolyte leakage in rice leaf segments exposed to 755.9 μM of oxadiazon, as well as, the imbibition of seeds with 200 μM of SNP for 10 hours increased by 33.1% the total content of chlorophyll, maintaining the pigmentation of seedlings exposed to 3.4 L h-1 of clomazone, applied to the needle stage. When sprayed on the shoot, paraquat + diuron, oxyfluorfen and oxadiazon, respectively, caused the ions leakage of 89.8; 62.8 and 18.3%, while the pre-treatment of the plants with 2000 μM of SNP decreased by 11 and 25.4% the damage caused by the first two, however, there was not reduction in injury caused by oxadiazon. The pre-treatment with SNP minimized the oxidative stress caused by herbicides, by the reducing in the hydrogen peroxide levels and TBARS and by the increase in the pigments levels, reflecting on the lower activity of the enzymes SOD and POD, however, such changes are dependent on the SNP concentration, exposure time and the level of stress caused by herbicides. In the absence or low levels of stress, NO was toxic to plants.Embora seletivos, alguns herbicidas causam injúrias nas plantas, resultando na produção descontrolada de espécies reativas de oxigênio. O óxido nítrico (NO) é apontado como um redutor dos efeitos causados pelo estresse oxidativo em plantas, entretanto, seus mecanismos de mitigação ainda não são claros. Este trabalho teve por objetivos avaliar os mecanismos de proteção do óxido nítrico, fornecido através de nitroprussiato de sódio (SNP), em plantas de arroz da cultivar Puita INTA CL, expostas aos herbicidas clomazone (360 g i.a. L-1), oxyfluorfen (240 g i.a. L-1), oxadiazon (250 g i.a. L-1) e à mistura formulada de paraquat (200 g i.a. L-1) + diuron (100 g i.a. L-1), in vitro e in vivo. Para tal, (I) segmentos foliares foram pré-incubados com 0, 200 e 2000 μM de SNP e expostos a cinco concentrações dos herbicidas, sendo o efeito protetor confirmado pelo seqüestrador de NO, carboxyPTIO, (II) sementes foram embebidas, por uma e 10 horas, em solução contendo 200 μM de SNP e após a semeadura, clomazone (1,7 e 3,4 L p.c. ha-1) foi aplicado em pós-semeadura e no ponto de agulha, (III) plantas foram tratadas com 200 e 2000 μM de SNP e pulverizadas com 1,0 L ha-1 dos herbicidas oxadiazon, oxyfluorfen e paraquat + diuron. A concentração de 200 μM de SNP reduziu, em até 14%, o extravasamento de eletrólitos em segmentos foliares de arroz expostos a 755,9 μM de oxadiazon, bem como, a embebição das sementes em 200 μM de SNP por 10 horas aumentou em 33,1% o teor de clorofila total, mantendo a pigmentação das plântulas expostas a 3,4 L ha-1 de clomazone, aplicado no ponto de agulha. Quando pulverizados na parte aérea das plantas, paraquat + diuron, oxyfluorfen e oxadiazon, respectivamente, causaram o extravasamento de 89,8; 62,8 e 18,3%, dos íons celulares, enquanto que as plantas pré-tratadas com 2000 μM de SNP apresentaram redução de 11 e 25,4% no dano causado pelos dois primeiros, contudo, sem redução na injúria causada por oxadiazon. O pré-tratamento com SNP minimizou o estresse oxidativo causado pelos herbicidas, através da redução dos níveis de peróxido de hidrogênio e Tbars e do aumento nos níveis de pigmentos, refletindo na menor atividade das enzimas SOD e POD, entretanto, tais alterações foram dependentes da concentração de SNP, tempo de exposição e do nível de estresse causado pelos herbicidas. Na ausência ou em baixos níveis de estresse, o NO mostrou-se tóxico às plantas.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em AgronomiaUFSMBRAgronomiaProteção de plantasAntioxidantesEspécies reativas de oxigênioPlant protectionAntioxidantsReactive species of oxygenCNPQ::CIENCIAS AGRARIAS::AGRONOMIAProteção do óxido nítrico sobre o estresse oxidativo causado por herbicidas em arroz irrigadoProtection of nitric oxide on the oxidative stress caused by herbicides in the flooded riceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisMachado, Sérgio Luiz de Oliveirahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780229P3Tabaldi, Luciane Almerihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4766007A6Nunes, Ubirajara Russihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4709066J0Mertz, Liliane Marciahttp://lattes.cnpq.br/2295885344548513Menezes, Nilson Lemos dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788277Y1http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4207798Z0Silva, Keli Souza da500100000009400300300300300300300e60531fa-cb8f-416b-b6ca-73f0fb0c0428e6305dc8-f61e-43d4-8952-847330802f7f6ab2d9a4-9715-4937-88f9-46c8258fa9c65d1edb2d-76d4-497a-946f-d4ba747059f3d70ff327-5845-4a17-a707-ffca40d28e7789918abc-aab5-4cfc-a154-c9cad26c7606info:eu-repo/semantics/openAccessreponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALSILVA, KELI SOUZA DA.pdfapplication/pdf1381918http://repositorio.ufsm.br/bitstream/1/3254/1/SILVA%2c%20KELI%20SOUZA%20DA.pdf8f3ea06748f1929dbdc29e1492f83bbcMD51TEXTSILVA, KELI SOUZA DA.pdf.txtSILVA, KELI SOUZA DA.pdf.txtExtracted texttext/plain169586http://repositorio.ufsm.br/bitstream/1/3254/2/SILVA%2c%20KELI%20SOUZA%20DA.pdf.txt5902038d019075d1431da8b8f4feb959MD52THUMBNAILSILVA, KELI SOUZA DA.pdf.jpgSILVA, KELI SOUZA DA.pdf.jpgIM Thumbnailimage/jpeg4728http://repositorio.ufsm.br/bitstream/1/3254/3/SILVA%2c%20KELI%20SOUZA%20DA.pdf.jpg135f2e3adf92b169370ce97c67207022MD531/32542017-07-25 10:53:03.566oai:repositorio.ufsm.br:1/3254Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestouvidoria@ufsm.bropendoar:39132017-07-25T13:53:03Repositório Institucional Manancial UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Proteção do óxido nítrico sobre o estresse oxidativo causado por herbicidas em arroz irrigado |
dc.title.alternative.eng.fl_str_mv |
Protection of nitric oxide on the oxidative stress caused by herbicides in the flooded rice |
title |
Proteção do óxido nítrico sobre o estresse oxidativo causado por herbicidas em arroz irrigado |
spellingShingle |
Proteção do óxido nítrico sobre o estresse oxidativo causado por herbicidas em arroz irrigado Silva, Keli Souza da Proteção de plantas Antioxidantes Espécies reativas de oxigênio Plant protection Antioxidants Reactive species of oxygen CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Proteção do óxido nítrico sobre o estresse oxidativo causado por herbicidas em arroz irrigado |
title_full |
Proteção do óxido nítrico sobre o estresse oxidativo causado por herbicidas em arroz irrigado |
title_fullStr |
Proteção do óxido nítrico sobre o estresse oxidativo causado por herbicidas em arroz irrigado |
title_full_unstemmed |
Proteção do óxido nítrico sobre o estresse oxidativo causado por herbicidas em arroz irrigado |
title_sort |
Proteção do óxido nítrico sobre o estresse oxidativo causado por herbicidas em arroz irrigado |
author |
Silva, Keli Souza da |
author_facet |
Silva, Keli Souza da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Machado, Sérgio Luiz de Oliveira |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780229P3 |
dc.contributor.referee1.fl_str_mv |
Tabaldi, Luciane Almeri |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4766007A6 |
dc.contributor.referee2.fl_str_mv |
Nunes, Ubirajara Russi |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4709066J0 |
dc.contributor.referee3.fl_str_mv |
Mertz, Liliane Marcia |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/2295885344548513 |
dc.contributor.referee4.fl_str_mv |
Menezes, Nilson Lemos de |
dc.contributor.referee4Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4788277Y1 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4207798Z0 |
dc.contributor.author.fl_str_mv |
Silva, Keli Souza da |
contributor_str_mv |
Machado, Sérgio Luiz de Oliveira Tabaldi, Luciane Almeri Nunes, Ubirajara Russi Mertz, Liliane Marcia Menezes, Nilson Lemos de |
dc.subject.por.fl_str_mv |
Proteção de plantas Antioxidantes Espécies reativas de oxigênio |
topic |
Proteção de plantas Antioxidantes Espécies reativas de oxigênio Plant protection Antioxidants Reactive species of oxygen CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
dc.subject.eng.fl_str_mv |
Plant protection Antioxidants Reactive species of oxygen |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
Although selective, some herbicides cause injury in plants, resulting in uncontrolled production of reactive oxygen species. Nitric oxide (NO) is appointed as a reducer of the effects caused by oxidative stress in plants; however, their mitigation mechanisms are not yet clear. This study aimed to evaluate the nitric oxide protection mechanisms, provided by sodium nitroprusside (SNP), in the flooded rice cultivar Puitá INTA CL exposed to clomazone herbicide (360 g a.i. L-1), oxyfluorfen (240 g a.i. L-1), oxadiazon (250 g a.i. L-1) and the formulated mixture of paraquat (200 g a.i. L-1) + diuron (100 g a.i. L-1) in vitro and in vivo. For this purpose, (i) leaf segments were pre-incubated with 0, 200 and 2000 μM of SNP and exposed to five herbicides concentrations, and the protective effect confirmed by the NO scavenger, carboxyPTIO, (ii) seeds were soaked by one and 10 hours in solution containing 200 μM of SNP and after sowing, clomazone (1.7 and 3.4 L ha-1 of c.p.) was applied in the post-seeding and the needle stage (iii) plants were treated with 200 and 2000 μM of SNP and sprayed with 1.0 L ha-1 of herbicides oxadiazon, oxyfluorfen + diuron and paraquat. The concentration of 200 μM of SNP has reduced, up to 14% the electrolyte leakage in rice leaf segments exposed to 755.9 μM of oxadiazon, as well as, the imbibition of seeds with 200 μM of SNP for 10 hours increased by 33.1% the total content of chlorophyll, maintaining the pigmentation of seedlings exposed to 3.4 L h-1 of clomazone, applied to the needle stage. When sprayed on the shoot, paraquat + diuron, oxyfluorfen and oxadiazon, respectively, caused the ions leakage of 89.8; 62.8 and 18.3%, while the pre-treatment of the plants with 2000 μM of SNP decreased by 11 and 25.4% the damage caused by the first two, however, there was not reduction in injury caused by oxadiazon. The pre-treatment with SNP minimized the oxidative stress caused by herbicides, by the reducing in the hydrogen peroxide levels and TBARS and by the increase in the pigments levels, reflecting on the lower activity of the enzymes SOD and POD, however, such changes are dependent on the SNP concentration, exposure time and the level of stress caused by herbicides. In the absence or low levels of stress, NO was toxic to plants. |
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2015 |
dc.date.issued.fl_str_mv |
2015-07-31 |
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2017-05-10 |
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2017-05-10 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Keli Souza da. Protection of nitric oxide on the oxidative stress caused by herbicides in the flooded rice. 2015. 87 f. Tese (Doutorado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2015. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/3254 |
identifier_str_mv |
SILVA, Keli Souza da. Protection of nitric oxide on the oxidative stress caused by herbicides in the flooded rice. 2015. 87 f. Tese (Doutorado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2015. |
url |
http://repositorio.ufsm.br/handle/1/3254 |
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por |
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Universidade Federal de Santa Maria |
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UFSM |
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Agronomia |
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Universidade Federal de Santa Maria |
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