Aspectos da qualidade de sementes e do estabelecimento in vitro de Eugenia involucrata de candolle
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/8778 |
Resumo: | Eugenia involucrata DC. is a native forest species in Brazil, and other countries in South America such as Argentina, Uruguay and Paraguay, with important economic, ecological and silvicultural. The species has edible fruit is widely used in cookery and has medicinal properties. Its seeds are recalcitrant, losing the viability and germination after collecting fruit, not supporting, thus avoiding long periods of storage. Because of this, we need studies to know the characteristics of the species, aiming at the production of quality seedlings. Thus, the overall objective of this study was to evaluate methodologies that can contribute to the propagation of Eugenia involucrata. They were evaluated the health quality, physical and physiological two lots of seeds, and different agents disinfectants for aseptic in vitro germination of seeds and different substrates, in order to ex vitro germination of the species. For in vitro establishment of nodal segments Eugenia involucrata were exposed to different disinfectants agents for the control of microbial contamination. Moreover, they also tested different pH values, sucrose and agar. The results indicated that Eugenia involucrata seeds present variations regarding the biometric features, high humidity and association with fungi. The filter paper substrate was not efficient for the germination of seeds of both lots evaluated. The vermiculite and grit:vermiculite in the ratio 1:1, gave the highest average of emerged seedlings. However, the seedlings did not develop appropriately if the vermiculite was disposed on the seeds, suggesting additional studies in this regard. For the in vitro establishment of nodal segments, tested disinfectants agents significantly decreased the fungal contamination in the explants, recommending the use of disinfectants agents Ca(ClO)2 3,0% (v/v) combined with NaClO 2,0% (v/v), however, these concentrations have not managed satisfactorily bacterial contamination. The pH was adjusted to 6,0 proved most efficient establishment of the explants in vitro. One can use 10g L-1 sucrose and 4g L-1 agar for the in vitro establishment phase nodal segments of Eugenia involucrata. |
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2016-07-182016-07-182016-02-19STEFANEL, Charlene Moro. ASPECTS OF SEED QUALITY AND THE ESTABLISHMENT IN VITRO OF Eugenia involucrata de Candolle. 2016. 101 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2016.http://repositorio.ufsm.br/handle/1/8778Eugenia involucrata DC. is a native forest species in Brazil, and other countries in South America such as Argentina, Uruguay and Paraguay, with important economic, ecological and silvicultural. The species has edible fruit is widely used in cookery and has medicinal properties. Its seeds are recalcitrant, losing the viability and germination after collecting fruit, not supporting, thus avoiding long periods of storage. Because of this, we need studies to know the characteristics of the species, aiming at the production of quality seedlings. Thus, the overall objective of this study was to evaluate methodologies that can contribute to the propagation of Eugenia involucrata. They were evaluated the health quality, physical and physiological two lots of seeds, and different agents disinfectants for aseptic in vitro germination of seeds and different substrates, in order to ex vitro germination of the species. For in vitro establishment of nodal segments Eugenia involucrata were exposed to different disinfectants agents for the control of microbial contamination. Moreover, they also tested different pH values, sucrose and agar. The results indicated that Eugenia involucrata seeds present variations regarding the biometric features, high humidity and association with fungi. The filter paper substrate was not efficient for the germination of seeds of both lots evaluated. The vermiculite and grit:vermiculite in the ratio 1:1, gave the highest average of emerged seedlings. However, the seedlings did not develop appropriately if the vermiculite was disposed on the seeds, suggesting additional studies in this regard. For the in vitro establishment of nodal segments, tested disinfectants agents significantly decreased the fungal contamination in the explants, recommending the use of disinfectants agents Ca(ClO)2 3,0% (v/v) combined with NaClO 2,0% (v/v), however, these concentrations have not managed satisfactorily bacterial contamination. The pH was adjusted to 6,0 proved most efficient establishment of the explants in vitro. One can use 10g L-1 sucrose and 4g L-1 agar for the in vitro establishment phase nodal segments of Eugenia involucrata.Eugenia involucrata DC. é uma espécie florestal nativa do Brasil, além de outros países da América do Sul, como Argentina, Uruguai e Paraguai, apresentando importância econômica, silvicultural e ecológica. A espécie possui frutos comestíveis sendo muito utilizados na culinária e apresenta propriedades medicinais. Suas sementes são recalcitrantes, perdendo a viabilidade e o poder germinativo após a coleta dos frutos, não suportando, assim, longos períodos de armazenamento. Por conta disso, são necessários estudos que permitam conhecer as características da espécie, visando a produção de mudas de qualidade. Desta maneira, o objetivo geral do presente trabalho foi avaliar metodologias que possam contribuir para a propagação vegetativa de Eugenia involucrata. Foram avaliados aspectos da qualidade sanitária, física e fisiológica de dois lotes de sementes, além de diferentes agentes desinfestantes para a germinação asséptica in vitro de sementes e diferentes substratos, visando à germinação ex vitro da espécie. Para o estabelecimento in vitro, segmentos nodais de Eugenia involucrata foram expostos a diferentes agentes desinfestantes, visando o controle da contaminação microbiana. Além disso, foram testados, também, diferentes valores de pH, sacarose e ágar. Os resultados obtidos indicaram que as sementes de Eugenia involucrata apresentam variações quanto aos aspectos biométricos, alto grau de umidade e associação com fungos. O substrato papel filtro não se mostrou eficiente para a germinação das sementes de ambos os lotes avaliados. Os substratos vermiculita e areia:vermiculita na proporção 1:1, proporcionaram as maiores médias de plântulas emergidas. No entanto, as plântulas não se desenvolveram de maneira adequada quando a vermiculita estava disposta sobre as sementes, sugerindo estudos adicionais a esse respeito. Para o estabelecimento in vitro de segmentos nodais, os agentes desinfestantes testados diminuíram significativamente a contaminação fúngica nos explantes, recomendando-se a utilização dos agentes desinfestantes Ca(ClO)2 a 3,0% (v/v) combinado a NaClO a 2,0% (v/v), entretanto, essas concentrações não controlaram de maneira satisfatória a contaminação bacteriana. O pH ajustado para 6,0 mostrou-se mais eficiente ao estabelecimento in vitro dos explantes. Pode-se utilizar 10g L-1 de sacarose e 4g L-1 de ágar durante a fase de estabelecimento in vitro de segmentos nodais de Eugenia involucrata.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Engenharia FlorestalUFSMBRRecursos Florestais e Engenharia FlorestalCerejeira-do-matoMyrtaceaeBiotecnologia florestalCherryMyrtaceaeForest biotechnologyCNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTALAspectos da qualidade de sementes e do estabelecimento in vitro de Eugenia involucrata de candolleAspects of seed quality and the establishment in vitro of Eugenia involucrata de Candolleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisReiniger, Lia Rejane Silveirahttp://lattes.cnpq.br/5739294882585391Paim, Aline Ferreirahttp://lattes.cnpq.br/5813893425276768Golle, Diego Pascoalhttp://lattes.cnpq.br/4193267565056836http://lattes.cnpq.br/7120227461634601Stefanel, Charlene Moro500200000003400500300300500809860fd-9f1f-4d0e-81fb-36d7e9781d1c0eb43e8c-f2b3-4523-843e-cefcafe3fc04df2a3c09-adf3-4326-ba66-7c8905cd6aacfadb2ed4-f587-4307-a4bc-1f2263b54387info:eu-repo/semantics/openAccessreponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALSTEFANEL, CHARLENE MORO.pdfapplication/pdf1265188http://repositorio.ufsm.br/bitstream/1/8778/1/STEFANEL%2c%20CHARLENE%20MORO.pdf3414bdfce3d70279dc9700d8aa1b3e02MD51TEXTSTEFANEL, CHARLENE MORO.pdf.txtSTEFANEL, CHARLENE MORO.pdf.txtExtracted texttext/plain199926http://repositorio.ufsm.br/bitstream/1/8778/2/STEFANEL%2c%20CHARLENE%20MORO.pdf.txt88561c5add5e181a32ba2080dc8f83c4MD52THUMBNAILSTEFANEL, CHARLENE MORO.pdf.jpgSTEFANEL, CHARLENE MORO.pdf.jpgIM Thumbnailimage/jpeg4376http://repositorio.ufsm.br/bitstream/1/8778/3/STEFANEL%2c%20CHARLENE%20MORO.pdf.jpgba3ffefa90afd6fdae8c28f90d678f39MD531/87782021-12-24 10:34:23.21oai:repositorio.ufsm.br:1/8778Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestouvidoria@ufsm.bropendoar:39132021-12-24T13:34:23Repositório Institucional Manancial UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Aspectos da qualidade de sementes e do estabelecimento in vitro de Eugenia involucrata de candolle |
dc.title.alternative.eng.fl_str_mv |
Aspects of seed quality and the establishment in vitro of Eugenia involucrata de Candolle |
title |
Aspectos da qualidade de sementes e do estabelecimento in vitro de Eugenia involucrata de candolle |
spellingShingle |
Aspectos da qualidade de sementes e do estabelecimento in vitro de Eugenia involucrata de candolle Stefanel, Charlene Moro Cerejeira-do-mato Myrtaceae Biotecnologia florestal Cherry Myrtaceae Forest biotechnology CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
title_short |
Aspectos da qualidade de sementes e do estabelecimento in vitro de Eugenia involucrata de candolle |
title_full |
Aspectos da qualidade de sementes e do estabelecimento in vitro de Eugenia involucrata de candolle |
title_fullStr |
Aspectos da qualidade de sementes e do estabelecimento in vitro de Eugenia involucrata de candolle |
title_full_unstemmed |
Aspectos da qualidade de sementes e do estabelecimento in vitro de Eugenia involucrata de candolle |
title_sort |
Aspectos da qualidade de sementes e do estabelecimento in vitro de Eugenia involucrata de candolle |
author |
Stefanel, Charlene Moro |
author_facet |
Stefanel, Charlene Moro |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Reiniger, Lia Rejane Silveira |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5739294882585391 |
dc.contributor.referee1.fl_str_mv |
Paim, Aline Ferreira |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/5813893425276768 |
dc.contributor.referee2.fl_str_mv |
Golle, Diego Pascoal |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/4193267565056836 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7120227461634601 |
dc.contributor.author.fl_str_mv |
Stefanel, Charlene Moro |
contributor_str_mv |
Reiniger, Lia Rejane Silveira Paim, Aline Ferreira Golle, Diego Pascoal |
dc.subject.por.fl_str_mv |
Cerejeira-do-mato Myrtaceae Biotecnologia florestal |
topic |
Cerejeira-do-mato Myrtaceae Biotecnologia florestal Cherry Myrtaceae Forest biotechnology CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
dc.subject.eng.fl_str_mv |
Cherry Myrtaceae Forest biotechnology |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
description |
Eugenia involucrata DC. is a native forest species in Brazil, and other countries in South America such as Argentina, Uruguay and Paraguay, with important economic, ecological and silvicultural. The species has edible fruit is widely used in cookery and has medicinal properties. Its seeds are recalcitrant, losing the viability and germination after collecting fruit, not supporting, thus avoiding long periods of storage. Because of this, we need studies to know the characteristics of the species, aiming at the production of quality seedlings. Thus, the overall objective of this study was to evaluate methodologies that can contribute to the propagation of Eugenia involucrata. They were evaluated the health quality, physical and physiological two lots of seeds, and different agents disinfectants for aseptic in vitro germination of seeds and different substrates, in order to ex vitro germination of the species. For in vitro establishment of nodal segments Eugenia involucrata were exposed to different disinfectants agents for the control of microbial contamination. Moreover, they also tested different pH values, sucrose and agar. The results indicated that Eugenia involucrata seeds present variations regarding the biometric features, high humidity and association with fungi. The filter paper substrate was not efficient for the germination of seeds of both lots evaluated. The vermiculite and grit:vermiculite in the ratio 1:1, gave the highest average of emerged seedlings. However, the seedlings did not develop appropriately if the vermiculite was disposed on the seeds, suggesting additional studies in this regard. For the in vitro establishment of nodal segments, tested disinfectants agents significantly decreased the fungal contamination in the explants, recommending the use of disinfectants agents Ca(ClO)2 3,0% (v/v) combined with NaClO 2,0% (v/v), however, these concentrations have not managed satisfactorily bacterial contamination. The pH was adjusted to 6,0 proved most efficient establishment of the explants in vitro. One can use 10g L-1 sucrose and 4g L-1 agar for the in vitro establishment phase nodal segments of Eugenia involucrata. |
publishDate |
2016 |
dc.date.accessioned.fl_str_mv |
2016-07-18 |
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2016-07-18 |
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2016-02-19 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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STEFANEL, Charlene Moro. ASPECTS OF SEED QUALITY AND THE ESTABLISHMENT IN VITRO OF Eugenia involucrata de Candolle. 2016. 101 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2016. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/8778 |
identifier_str_mv |
STEFANEL, Charlene Moro. ASPECTS OF SEED QUALITY AND THE ESTABLISHMENT IN VITRO OF Eugenia involucrata de Candolle. 2016. 101 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2016. |
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http://repositorio.ufsm.br/handle/1/8778 |
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