Influência da ingestão crônica de gordura vegetal hidrogenada sobre aspectos bioquímico-moleculares no estriado de ratos expostos a um modelo animal de mania
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/9027 |
Resumo: | Taking into account the unknow etiology of neuropsychiatry disturbances, including bipolar disorder (BD) and the bad eating habbits, the purpose of this study was to evaluate the influence of chronic consumption of trans fat, remaining in a large amount in processed foods, on lipid composition of striatal membranes, as well as its biochemical-molecular alterations in this tissue. To perform this study, a second generation of male rats born from mothers and grandmothers supplemented with soybean oil (SO-C, an isocaloric control group) or hydrogenated vegetable fat (HVF, rich in TFA) (3 g/kg; p.o.) since gestational period until adulthood was used. They were kept under oral treatment until adulthood, when they were exposed to an AMPH-induced model of mania (4 mg/Kg/ml/day, i.p., during 14 days). The HVF group presented 0.38% of TFA incorporation in the striatum, affecting Na+/K+ ATPase enzyme activity, which was decreased per se and following AMPH-exposure. The HVF group also showed increased protein carbonyl (PC) and brain-derived neurotrophic factor (BDNF) mRNA levels after AMPH administration, while these oxidative and molecular changes were not observed in the other experimental groups. Additionally, a negative correlation between striatal Na+/K+ ATPase activity and PC levels (r2= 0.49) was observed. The prolonged consumption of HVF, rich in TFA, allowed that FA incorporation and increases striatal oxidative status, thus impairing the functionality of Na+/K+ATPase and affecting molecular targets as BDNF mRNA. We hypothesized that the chronic intake of processed foods (rich in TFA) facilitates the development of neuropsychiatric diseases, particularly the bipolar disorder. |
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Influência da ingestão crônica de gordura vegetal hidrogenada sobre aspectos bioquímico-moleculares no estriado de ratos expostos a um modelo animal de maniaInfluence of hydrogenated vegetable fat chronic consumption on biochemical-molecular aspects in striatum of rats exposed to an animal model of maniaÁcidos graxos transAnfetaminaBDNFSegunda geraçãoTrans fat acidsAmphetamineSecond generationCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIATaking into account the unknow etiology of neuropsychiatry disturbances, including bipolar disorder (BD) and the bad eating habbits, the purpose of this study was to evaluate the influence of chronic consumption of trans fat, remaining in a large amount in processed foods, on lipid composition of striatal membranes, as well as its biochemical-molecular alterations in this tissue. To perform this study, a second generation of male rats born from mothers and grandmothers supplemented with soybean oil (SO-C, an isocaloric control group) or hydrogenated vegetable fat (HVF, rich in TFA) (3 g/kg; p.o.) since gestational period until adulthood was used. They were kept under oral treatment until adulthood, when they were exposed to an AMPH-induced model of mania (4 mg/Kg/ml/day, i.p., during 14 days). The HVF group presented 0.38% of TFA incorporation in the striatum, affecting Na+/K+ ATPase enzyme activity, which was decreased per se and following AMPH-exposure. The HVF group also showed increased protein carbonyl (PC) and brain-derived neurotrophic factor (BDNF) mRNA levels after AMPH administration, while these oxidative and molecular changes were not observed in the other experimental groups. Additionally, a negative correlation between striatal Na+/K+ ATPase activity and PC levels (r2= 0.49) was observed. The prolonged consumption of HVF, rich in TFA, allowed that FA incorporation and increases striatal oxidative status, thus impairing the functionality of Na+/K+ATPase and affecting molecular targets as BDNF mRNA. We hypothesized that the chronic intake of processed foods (rich in TFA) facilitates the development of neuropsychiatric diseases, particularly the bipolar disorder.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorLevando em consideração a etiologia desconhecida dos transtornos neuropsiquiátricos, incluindo o transtorno bipolar (TB) e os maus hábitos alimentares, o propósito desse estudo foi avaliar a influência do consumo crônico de gordura trans, que ainda se encontra presente em grandes quantidades em alimentos processados, sobre a natureza das membranas estriatais de ratos, assim como alterações bioquímico-moleculares nesse tecido, em ratos expostos a um modelo animal de mania. Para a realização este estudo, a 2a geração de ratos machos nascidos de mães e avós suplementadas com óleo de soja (OS-C) ou gordura vegetal hidrogenada (GVH) (3 g/kg; via oral) desde o período gestacional até a idade adulta foram utilizados. Eles foram mantidos sob a mesma suplementação de suas mães até a idade adulta, quando foram expostos a um modelo animal de mania induzido por anfetamina (ANF) (4 mg/Kg/ml/dia, i.p.; durante 14 dias). O grupo suplementado com GVH apresentou uma incorporação de 0.38% de ácidos graxos trans (AGT) no estriado comparado com o grupo OS-C, afetando a atividade da enzima Na+/K+ ATPase, que foi diminuída per se e após a administração de ANF. Além disso, o grupo suplementado com GVH apresentou níveis aumentados de proteína carbonil (PC) e do RNAm do fator neurotrófico derivado do encéfalo (BDNF) após administração de ANF, o que não foi observado nos outros grupos experimentais. Adicionalmente, uma correlação negativa entre a atividade estriatal da enzima Na+/K+ ATPase e níveis de PC (r2=0.49) foi observada. O prolongado consumo de GVH, rica em AGT, permitiu a incorporação destes AG e aumentou o status oxidativo estriatal, comprometendo a atividade da Na+/K+ ATPase e afetando alvos moleculares como RNAm do BDNF. Nós hipotetizamos que a ingesta crônica de alimentos processados (ricos em AGT) pode facilitar o desenvolvimento de doenças neuropsiquiátricas, entre elas o transtorno bipolar.Universidade Federal de Santa MariaBRFarmacologiaUFSMPrograma de Pós-Graduação em FarmacologiaTrevizol, Fabíolahttp://lattes.cnpq.br/3002178545420252Oliveira, Sara Marchesan dehttp://lattes.cnpq.br/6574555059806902Posser, Thaishttp://lattes.cnpq.br/2277857386983441Dias, Verônica Tironi2016-05-092016-05-092015-11-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfDIAS, Verônica Tironi. Influence of hydrogenated vegetable fat chronic consumption on biochemical-molecular aspects in striatum of rats exposed to an animal model of mania. 2015. 50 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2015.http://repositorio.ufsm.br/handle/1/9027porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-05-02T14:11:05Zoai:repositorio.ufsm.br:1/9027Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-05-02T14:11:05Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Influência da ingestão crônica de gordura vegetal hidrogenada sobre aspectos bioquímico-moleculares no estriado de ratos expostos a um modelo animal de mania Influence of hydrogenated vegetable fat chronic consumption on biochemical-molecular aspects in striatum of rats exposed to an animal model of mania |
title |
Influência da ingestão crônica de gordura vegetal hidrogenada sobre aspectos bioquímico-moleculares no estriado de ratos expostos a um modelo animal de mania |
spellingShingle |
Influência da ingestão crônica de gordura vegetal hidrogenada sobre aspectos bioquímico-moleculares no estriado de ratos expostos a um modelo animal de mania Dias, Verônica Tironi Ácidos graxos trans Anfetamina BDNF Segunda geração Trans fat acids Amphetamine Second generation CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
title_short |
Influência da ingestão crônica de gordura vegetal hidrogenada sobre aspectos bioquímico-moleculares no estriado de ratos expostos a um modelo animal de mania |
title_full |
Influência da ingestão crônica de gordura vegetal hidrogenada sobre aspectos bioquímico-moleculares no estriado de ratos expostos a um modelo animal de mania |
title_fullStr |
Influência da ingestão crônica de gordura vegetal hidrogenada sobre aspectos bioquímico-moleculares no estriado de ratos expostos a um modelo animal de mania |
title_full_unstemmed |
Influência da ingestão crônica de gordura vegetal hidrogenada sobre aspectos bioquímico-moleculares no estriado de ratos expostos a um modelo animal de mania |
title_sort |
Influência da ingestão crônica de gordura vegetal hidrogenada sobre aspectos bioquímico-moleculares no estriado de ratos expostos a um modelo animal de mania |
author |
Dias, Verônica Tironi |
author_facet |
Dias, Verônica Tironi |
author_role |
author |
dc.contributor.none.fl_str_mv |
Trevizol, Fabíola http://lattes.cnpq.br/3002178545420252 Oliveira, Sara Marchesan de http://lattes.cnpq.br/6574555059806902 Posser, Thais http://lattes.cnpq.br/2277857386983441 |
dc.contributor.author.fl_str_mv |
Dias, Verônica Tironi |
dc.subject.por.fl_str_mv |
Ácidos graxos trans Anfetamina BDNF Segunda geração Trans fat acids Amphetamine Second generation CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
topic |
Ácidos graxos trans Anfetamina BDNF Segunda geração Trans fat acids Amphetamine Second generation CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
description |
Taking into account the unknow etiology of neuropsychiatry disturbances, including bipolar disorder (BD) and the bad eating habbits, the purpose of this study was to evaluate the influence of chronic consumption of trans fat, remaining in a large amount in processed foods, on lipid composition of striatal membranes, as well as its biochemical-molecular alterations in this tissue. To perform this study, a second generation of male rats born from mothers and grandmothers supplemented with soybean oil (SO-C, an isocaloric control group) or hydrogenated vegetable fat (HVF, rich in TFA) (3 g/kg; p.o.) since gestational period until adulthood was used. They were kept under oral treatment until adulthood, when they were exposed to an AMPH-induced model of mania (4 mg/Kg/ml/day, i.p., during 14 days). The HVF group presented 0.38% of TFA incorporation in the striatum, affecting Na+/K+ ATPase enzyme activity, which was decreased per se and following AMPH-exposure. The HVF group also showed increased protein carbonyl (PC) and brain-derived neurotrophic factor (BDNF) mRNA levels after AMPH administration, while these oxidative and molecular changes were not observed in the other experimental groups. Additionally, a negative correlation between striatal Na+/K+ ATPase activity and PC levels (r2= 0.49) was observed. The prolonged consumption of HVF, rich in TFA, allowed that FA incorporation and increases striatal oxidative status, thus impairing the functionality of Na+/K+ATPase and affecting molecular targets as BDNF mRNA. We hypothesized that the chronic intake of processed foods (rich in TFA) facilitates the development of neuropsychiatric diseases, particularly the bipolar disorder. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-11-27 2016-05-09 2016-05-09 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
DIAS, Verônica Tironi. Influence of hydrogenated vegetable fat chronic consumption on biochemical-molecular aspects in striatum of rats exposed to an animal model of mania. 2015. 50 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2015. http://repositorio.ufsm.br/handle/1/9027 |
identifier_str_mv |
DIAS, Verônica Tironi. Influence of hydrogenated vegetable fat chronic consumption on biochemical-molecular aspects in striatum of rats exposed to an animal model of mania. 2015. 50 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2015. |
url |
http://repositorio.ufsm.br/handle/1/9027 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Farmacologia UFSM Programa de Pós-Graduação em Farmacologia |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Farmacologia UFSM Programa de Pós-Graduação em Farmacologia |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922122193698816 |