Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysis
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600454 |
Resumo: | ABSTRACT: The study aimed to evaluate the equipment Ekomilk Scan® as an alternative to somatic cell count (SCC) in milk. For this individual cow milk samples of various ages and different stages of lactation in northeastern state of São Paulo region were collected. The analyzes performed were divided into variables related to the equipment: repeatability and reproducibility, and variables that could influencing the results as: use of preservatives, temperature, time between collection and analysis, breed and milk composition, besides analysis to relate the Ekomilk Scan® with the standard method-direct microscopy and reference-flow cytometry. As the result, for samples analysis, it shouldn’t be added preservative and these should be conducted on the same day of collection; however, temperature sample did not significantly influence results. Furthermore, Ekomilk Scan® did not show good correlation of results with the method of direct microscopy; however, it was necessary to generate equations for a positive correlation between flow cytometry and Ekomilk Scan®. Therefore, it is concluded that the equipment tested is not accurate but it can be an alternative for SCC monitoring in productive units since it uses calibration equations of results. |
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Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysismonitoringudder healthalternative methodsomatic cell countABSTRACT: The study aimed to evaluate the equipment Ekomilk Scan® as an alternative to somatic cell count (SCC) in milk. For this individual cow milk samples of various ages and different stages of lactation in northeastern state of São Paulo region were collected. The analyzes performed were divided into variables related to the equipment: repeatability and reproducibility, and variables that could influencing the results as: use of preservatives, temperature, time between collection and analysis, breed and milk composition, besides analysis to relate the Ekomilk Scan® with the standard method-direct microscopy and reference-flow cytometry. As the result, for samples analysis, it shouldn’t be added preservative and these should be conducted on the same day of collection; however, temperature sample did not significantly influence results. Furthermore, Ekomilk Scan® did not show good correlation of results with the method of direct microscopy; however, it was necessary to generate equations for a positive correlation between flow cytometry and Ekomilk Scan®. Therefore, it is concluded that the equipment tested is not accurate but it can be an alternative for SCC monitoring in productive units since it uses calibration equations of results.Universidade Federal de Santa Maria2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600454Ciência Rural v.48 n.6 2018reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20170848info:eu-repo/semantics/openAccessGonçalves,Ana Carolina SiqueiraRoma Júnior,Luiz CarlosPrivatti,Rafaela TorrezanSalles,Márcia Saladini VieiraPaz,Claudia Cristina Paro deZadra,Lenira El FaroVidal,Ana Maria Centolaeng2018-06-19T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysis |
title |
Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysis |
spellingShingle |
Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysis Gonçalves,Ana Carolina Siqueira monitoring udder health alternative method somatic cell count |
title_short |
Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysis |
title_full |
Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysis |
title_fullStr |
Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysis |
title_full_unstemmed |
Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysis |
title_sort |
Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysis |
author |
Gonçalves,Ana Carolina Siqueira |
author_facet |
Gonçalves,Ana Carolina Siqueira Roma Júnior,Luiz Carlos Privatti,Rafaela Torrezan Salles,Márcia Saladini Vieira Paz,Claudia Cristina Paro de Zadra,Lenira El Faro Vidal,Ana Maria Centola |
author_role |
author |
author2 |
Roma Júnior,Luiz Carlos Privatti,Rafaela Torrezan Salles,Márcia Saladini Vieira Paz,Claudia Cristina Paro de Zadra,Lenira El Faro Vidal,Ana Maria Centola |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Gonçalves,Ana Carolina Siqueira Roma Júnior,Luiz Carlos Privatti,Rafaela Torrezan Salles,Márcia Saladini Vieira Paz,Claudia Cristina Paro de Zadra,Lenira El Faro Vidal,Ana Maria Centola |
dc.subject.por.fl_str_mv |
monitoring udder health alternative method somatic cell count |
topic |
monitoring udder health alternative method somatic cell count |
description |
ABSTRACT: The study aimed to evaluate the equipment Ekomilk Scan® as an alternative to somatic cell count (SCC) in milk. For this individual cow milk samples of various ages and different stages of lactation in northeastern state of São Paulo region were collected. The analyzes performed were divided into variables related to the equipment: repeatability and reproducibility, and variables that could influencing the results as: use of preservatives, temperature, time between collection and analysis, breed and milk composition, besides analysis to relate the Ekomilk Scan® with the standard method-direct microscopy and reference-flow cytometry. As the result, for samples analysis, it shouldn’t be added preservative and these should be conducted on the same day of collection; however, temperature sample did not significantly influence results. Furthermore, Ekomilk Scan® did not show good correlation of results with the method of direct microscopy; however, it was necessary to generate equations for a positive correlation between flow cytometry and Ekomilk Scan®. Therefore, it is concluded that the equipment tested is not accurate but it can be an alternative for SCC monitoring in productive units since it uses calibration equations of results. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600454 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600454 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20170848 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.48 n.6 2018 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140552563032064 |