Systemic and local antibodies induced by an experimental inactivated vaccine against bovine herpesvirus type 1

Detalhes bibliográficos
Autor(a) principal: Cilento,Maria do Carmo
Data de Publicação: 2011
Outros Autores: Pituco,Edviges Maristela, Jordão,Ricardo Spacagna, Ribeiro,Cláudia Pestana, Marchiori Filho,Moacir, Montassier,Hélio José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000200021
Resumo: An experimental inactivated vaccine against bovine herpesvirus-1 (BoHV-1) was produced aiming to evaluate the systemic and local antibody responses in 12 seronegative heifers, after vaccination and revaccination. Serum samples were submitted to virus neutralization assay and to ELISA test for detection of IgG1 and IgG2 isotypes. Nasal secretion samples were submitted to the same ELISA test for detection of IgG1 and IgG2 isotypes. The results showed that moderate to high neutralizing titres and IgG1 and IgG2 antibody responses were induced after the second vaccination in the serum and in nasal secretions up to 114 days post vaccination. IgG2 antibodies were the prevalent isotype for most of the post-vaccination period. The results indicate that BoHV-1 experimental inactivated vaccine elicited potentially protective IgG1 and IgG2 antibody levels, both in the systemic and mucosal compartments.
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spelling Systemic and local antibodies induced by an experimental inactivated vaccine against bovine herpesvirus type 1BoHV-1immunoglobulin isotypesexperimental vaccineAn experimental inactivated vaccine against bovine herpesvirus-1 (BoHV-1) was produced aiming to evaluate the systemic and local antibody responses in 12 seronegative heifers, after vaccination and revaccination. Serum samples were submitted to virus neutralization assay and to ELISA test for detection of IgG1 and IgG2 isotypes. Nasal secretion samples were submitted to the same ELISA test for detection of IgG1 and IgG2 isotypes. The results showed that moderate to high neutralizing titres and IgG1 and IgG2 antibody responses were induced after the second vaccination in the serum and in nasal secretions up to 114 days post vaccination. IgG2 antibodies were the prevalent isotype for most of the post-vaccination period. The results indicate that BoHV-1 experimental inactivated vaccine elicited potentially protective IgG1 and IgG2 antibody levels, both in the systemic and mucosal compartments.Universidade Federal de Santa Maria2011-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000200021Ciência Rural v.41 n.2 2011reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782011000200021info:eu-repo/semantics/openAccessCilento,Maria do CarmoPituco,Edviges MaristelaJordão,Ricardo SpacagnaRibeiro,Cláudia PestanaMarchiori Filho,MoacirMontassier,Hélio Joséeng2011-09-26T00:00:00ZRevista
dc.title.none.fl_str_mv Systemic and local antibodies induced by an experimental inactivated vaccine against bovine herpesvirus type 1
title Systemic and local antibodies induced by an experimental inactivated vaccine against bovine herpesvirus type 1
spellingShingle Systemic and local antibodies induced by an experimental inactivated vaccine against bovine herpesvirus type 1
Cilento,Maria do Carmo
BoHV-1
immunoglobulin isotypes
experimental vaccine
title_short Systemic and local antibodies induced by an experimental inactivated vaccine against bovine herpesvirus type 1
title_full Systemic and local antibodies induced by an experimental inactivated vaccine against bovine herpesvirus type 1
title_fullStr Systemic and local antibodies induced by an experimental inactivated vaccine against bovine herpesvirus type 1
title_full_unstemmed Systemic and local antibodies induced by an experimental inactivated vaccine against bovine herpesvirus type 1
title_sort Systemic and local antibodies induced by an experimental inactivated vaccine against bovine herpesvirus type 1
author Cilento,Maria do Carmo
author_facet Cilento,Maria do Carmo
Pituco,Edviges Maristela
Jordão,Ricardo Spacagna
Ribeiro,Cláudia Pestana
Marchiori Filho,Moacir
Montassier,Hélio José
author_role author
author2 Pituco,Edviges Maristela
Jordão,Ricardo Spacagna
Ribeiro,Cláudia Pestana
Marchiori Filho,Moacir
Montassier,Hélio José
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Cilento,Maria do Carmo
Pituco,Edviges Maristela
Jordão,Ricardo Spacagna
Ribeiro,Cláudia Pestana
Marchiori Filho,Moacir
Montassier,Hélio José
dc.subject.por.fl_str_mv BoHV-1
immunoglobulin isotypes
experimental vaccine
topic BoHV-1
immunoglobulin isotypes
experimental vaccine
description An experimental inactivated vaccine against bovine herpesvirus-1 (BoHV-1) was produced aiming to evaluate the systemic and local antibody responses in 12 seronegative heifers, after vaccination and revaccination. Serum samples were submitted to virus neutralization assay and to ELISA test for detection of IgG1 and IgG2 isotypes. Nasal secretion samples were submitted to the same ELISA test for detection of IgG1 and IgG2 isotypes. The results showed that moderate to high neutralizing titres and IgG1 and IgG2 antibody responses were induced after the second vaccination in the serum and in nasal secretions up to 114 days post vaccination. IgG2 antibodies were the prevalent isotype for most of the post-vaccination period. The results indicate that BoHV-1 experimental inactivated vaccine elicited potentially protective IgG1 and IgG2 antibody levels, both in the systemic and mucosal compartments.
publishDate 2011
dc.date.none.fl_str_mv 2011-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000200021
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000200021
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-84782011000200021
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.41 n.2 2011
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1749140538230046720

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