Atividade da NTPDase, 5 -nucleotidase, acetilcolinesterase e níveis de peroxidação lipídica em ratos expostos ao cádmio e tratados com curcumina
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/11243 |
Resumo: | Cadmium (Cd) is one of the most toxic metals in relation to environmental contamination and human poisoning. This metal has a long biological half-life (10-30 years) and prolonged exposure to Cd has been associated with toxic effects on many organs as kidneys, liver, lungs, bone, including the brain, probably due to generation of stress oxidative. In this context, it is known that the polyphenol curcumin, natural compound derived from Curcuma longa, exerce several therapeutic functions, highlighting the great antioxidant, anti-inflammatory and neuroprotective potential. Thus, this work aims to evaluate the behavioral and memory parameters, the activity of acetylcholinesterase and ectonzimas, as well as lipid peroxidation in rats intoxicated by Cd and to determine the possible protective effect of curcumin. For this, we used 120 male Wistar rats, that received CdCl2.H2O as cadmium (Cd; 1 mg/kg) and/or curcumin (Curc, 30, 60 or 90 mg/kg) five times a week during 3 months. The animals were randomly divided into 8 groups (n = 15): (1) Saline/oil (2) Saline/Curc 30 (3) Saline/Curc 60, (4) Saline/Curc 90 (5) Cd/oil (6) Cd/Curc 30 (7) Cd/Curc 60 and (8) Cd/Curc 90. The results showed an increase in the levels of lipid peroxidation and in the AChE activity in different cerebral structures. The Cd was also increase the AChE and NTPDase (ATP and ADP as substrate) and decrease the 5 -nucleotidase activity from cerebral cortex synaptosomes. These Cd effects on central nervous system may be associated with memory deficits, evidenced by decreasing in step-down latency in the inhibitory avoidance test. Moreover, Cd also increased peripheral AChE activity of AChE (whole blood and lymphocytes) which can be associated with a pro-inflammatory state in these animals. However, Curc was effective in reducing the harmful effects of Cd decreasing lipid peroxidation and preventing increased AChE and NTPDase activities, thereby improving neurotransmission and cognitives processes, suggesting that this natural compound may be considered, after further study, an important ally in therapies against poisoning by this metal. |
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Atividade da NTPDase, 5 -nucleotidase, acetilcolinesterase e níveis de peroxidação lipídica em ratos expostos ao cádmio e tratados com curcuminaActivity of NTPDase, 5'- nucleotidase, actylcholinesterase and peroxidation lipid levels in rats exposed to cadmium and treated with curcuminCádmioCurcuminaEcto-nucleotidasesAcetilcolinesterasePeroxidação lipídicaCadmiumCurcuminEcto-nucleotidasesAcetylcholinesteraseLipid peroxidationCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICACadmium (Cd) is one of the most toxic metals in relation to environmental contamination and human poisoning. This metal has a long biological half-life (10-30 years) and prolonged exposure to Cd has been associated with toxic effects on many organs as kidneys, liver, lungs, bone, including the brain, probably due to generation of stress oxidative. In this context, it is known that the polyphenol curcumin, natural compound derived from Curcuma longa, exerce several therapeutic functions, highlighting the great antioxidant, anti-inflammatory and neuroprotective potential. Thus, this work aims to evaluate the behavioral and memory parameters, the activity of acetylcholinesterase and ectonzimas, as well as lipid peroxidation in rats intoxicated by Cd and to determine the possible protective effect of curcumin. For this, we used 120 male Wistar rats, that received CdCl2.H2O as cadmium (Cd; 1 mg/kg) and/or curcumin (Curc, 30, 60 or 90 mg/kg) five times a week during 3 months. The animals were randomly divided into 8 groups (n = 15): (1) Saline/oil (2) Saline/Curc 30 (3) Saline/Curc 60, (4) Saline/Curc 90 (5) Cd/oil (6) Cd/Curc 30 (7) Cd/Curc 60 and (8) Cd/Curc 90. The results showed an increase in the levels of lipid peroxidation and in the AChE activity in different cerebral structures. The Cd was also increase the AChE and NTPDase (ATP and ADP as substrate) and decrease the 5 -nucleotidase activity from cerebral cortex synaptosomes. These Cd effects on central nervous system may be associated with memory deficits, evidenced by decreasing in step-down latency in the inhibitory avoidance test. Moreover, Cd also increased peripheral AChE activity of AChE (whole blood and lymphocytes) which can be associated with a pro-inflammatory state in these animals. However, Curc was effective in reducing the harmful effects of Cd decreasing lipid peroxidation and preventing increased AChE and NTPDase activities, thereby improving neurotransmission and cognitives processes, suggesting that this natural compound may be considered, after further study, an important ally in therapies against poisoning by this metal.Conselho Nacional de Desenvolvimento Científico e TecnológicoO cádmio (Cd) é um dos metais mais tóxicos tanto em nível de intoxicação humana quanto de contaminação ambiental. Pelo fato desse metal possuir uma longa meia-vida biológica (10-30 anos), a exposição prolongada a ele tem sido associada a efeitos danosos em muitos órgãos como rins, fígado, pulmões, ossos, e inclusive o cérebro, provavelmente devido à geração de estresse oxidativo. Neste contexto, sabe-se que o polifenol curcumina, composto natural obtido a partir de Curcuma longa, exerce diversas funções terapêuticas, destacando-se o grande potencial antioxidante, anti-inflamatório e neuroprotetor. Sendo assim, este trabalho visou avaliar os parâmetros comportamentais e de memória, a atividade das ectoenzimas e acetilcolinesterase, bem com a peroxidação lipídica em ratos intoxicados por Cd e o possível efeito protetor da curcumina. Para tanto, foram utilizados 120 ratos Wistar machos, que receberam cádmio na forma CdCl2.H2O (Cd; 1 mg/kg) e/ou curcumina (Curc; 30, 60 ou 90 mg/kg) cinco vezes por semana durante 3 meses. Os animais foram divididos ao acaso em 8 grupos (n=15): (1) Salina/óleo, (2) Salina/Curc 30, (3) Salina/Curc 60, (4) Salina/Curc 90, (5) Cd/óleo, (6) Cd/Curc 30, (7) Cd/Curc 60 e (8) Cd/Curc 90. Os resultados demonstraram um aumento nos níveis de peroxidação lipídica e na atividade da enzima AChE em diferentes estruturas encefálicas. A intoxicação por Cd também aumentou a atividade das enzimas AChE e NTPDase (substratos ATP e ADP) e diminuiu a atividade da enzima 5 -nucleotidade em sinaptossomas de córtex cerebral. Esses efeitos do Cd no sistema nervoso central podem estar associados ao déficit de memória, evidenciado através da diminuição do tempo de latência no teste de esquiva inibitória. Além disso, o Cd também aumentou a atividade da enzima AChE periférica (sangue total e linfócitos) o que pode estar relacionado com um estado pró-inflamatório nesses animais. No entanto, a Curc foi eficaz em reduzir os efeitos danosos do Cd, diminuindo a peroxidação lipídica nas estruturas cerebrais, prevenindo o aumento da atividade da AChE e da NTPDase, melhorando, assim, a neurotransmissão e os processos cognitivos, sugerindo que este composto natural possa ser considerado após estudos adicionais um importante aliado em terapias contra a intoxicação por esse metal.Universidade Federal de Santa MariaBRBioquímicaUFSMPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaMorsch, Vera Mariahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784273E6Leal, Daniela Bitencourt Rosahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4706487E7Oliveira, Sara Marchesan dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4268855J0Costa, Pauline da2015-11-112015-11-112014-10-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfCOSTA, Pauline da. ACTIVITY OF NTPDase, 5 - NUCLEOTIDASE, ACETYLCHOLINESTERASE AND PEROXIDATION LIPID LEVELS IN RATS EXPOSED TO CADMIUM AND TREATED WITH CURCUMIN. 2014. 91 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014.http://repositorio.ufsm.br/handle/1/11243porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2017-07-30T06:25:34Zoai:repositorio.ufsm.br:1/11243Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2017-07-30T06:25:34Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Atividade da NTPDase, 5 -nucleotidase, acetilcolinesterase e níveis de peroxidação lipídica em ratos expostos ao cádmio e tratados com curcumina Activity of NTPDase, 5'- nucleotidase, actylcholinesterase and peroxidation lipid levels in rats exposed to cadmium and treated with curcumin |
title |
Atividade da NTPDase, 5 -nucleotidase, acetilcolinesterase e níveis de peroxidação lipídica em ratos expostos ao cádmio e tratados com curcumina |
spellingShingle |
Atividade da NTPDase, 5 -nucleotidase, acetilcolinesterase e níveis de peroxidação lipídica em ratos expostos ao cádmio e tratados com curcumina Costa, Pauline da Cádmio Curcumina Ecto-nucleotidases Acetilcolinesterase Peroxidação lipídica Cadmium Curcumin Ecto-nucleotidases Acetylcholinesterase Lipid peroxidation CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Atividade da NTPDase, 5 -nucleotidase, acetilcolinesterase e níveis de peroxidação lipídica em ratos expostos ao cádmio e tratados com curcumina |
title_full |
Atividade da NTPDase, 5 -nucleotidase, acetilcolinesterase e níveis de peroxidação lipídica em ratos expostos ao cádmio e tratados com curcumina |
title_fullStr |
Atividade da NTPDase, 5 -nucleotidase, acetilcolinesterase e níveis de peroxidação lipídica em ratos expostos ao cádmio e tratados com curcumina |
title_full_unstemmed |
Atividade da NTPDase, 5 -nucleotidase, acetilcolinesterase e níveis de peroxidação lipídica em ratos expostos ao cádmio e tratados com curcumina |
title_sort |
Atividade da NTPDase, 5 -nucleotidase, acetilcolinesterase e níveis de peroxidação lipídica em ratos expostos ao cádmio e tratados com curcumina |
author |
Costa, Pauline da |
author_facet |
Costa, Pauline da |
author_role |
author |
dc.contributor.none.fl_str_mv |
Morsch, Vera Maria http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784273E6 Leal, Daniela Bitencourt Rosa http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4706487E7 Oliveira, Sara Marchesan de http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4268855J0 |
dc.contributor.author.fl_str_mv |
Costa, Pauline da |
dc.subject.por.fl_str_mv |
Cádmio Curcumina Ecto-nucleotidases Acetilcolinesterase Peroxidação lipídica Cadmium Curcumin Ecto-nucleotidases Acetylcholinesterase Lipid peroxidation CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
topic |
Cádmio Curcumina Ecto-nucleotidases Acetilcolinesterase Peroxidação lipídica Cadmium Curcumin Ecto-nucleotidases Acetylcholinesterase Lipid peroxidation CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Cadmium (Cd) is one of the most toxic metals in relation to environmental contamination and human poisoning. This metal has a long biological half-life (10-30 years) and prolonged exposure to Cd has been associated with toxic effects on many organs as kidneys, liver, lungs, bone, including the brain, probably due to generation of stress oxidative. In this context, it is known that the polyphenol curcumin, natural compound derived from Curcuma longa, exerce several therapeutic functions, highlighting the great antioxidant, anti-inflammatory and neuroprotective potential. Thus, this work aims to evaluate the behavioral and memory parameters, the activity of acetylcholinesterase and ectonzimas, as well as lipid peroxidation in rats intoxicated by Cd and to determine the possible protective effect of curcumin. For this, we used 120 male Wistar rats, that received CdCl2.H2O as cadmium (Cd; 1 mg/kg) and/or curcumin (Curc, 30, 60 or 90 mg/kg) five times a week during 3 months. The animals were randomly divided into 8 groups (n = 15): (1) Saline/oil (2) Saline/Curc 30 (3) Saline/Curc 60, (4) Saline/Curc 90 (5) Cd/oil (6) Cd/Curc 30 (7) Cd/Curc 60 and (8) Cd/Curc 90. The results showed an increase in the levels of lipid peroxidation and in the AChE activity in different cerebral structures. The Cd was also increase the AChE and NTPDase (ATP and ADP as substrate) and decrease the 5 -nucleotidase activity from cerebral cortex synaptosomes. These Cd effects on central nervous system may be associated with memory deficits, evidenced by decreasing in step-down latency in the inhibitory avoidance test. Moreover, Cd also increased peripheral AChE activity of AChE (whole blood and lymphocytes) which can be associated with a pro-inflammatory state in these animals. However, Curc was effective in reducing the harmful effects of Cd decreasing lipid peroxidation and preventing increased AChE and NTPDase activities, thereby improving neurotransmission and cognitives processes, suggesting that this natural compound may be considered, after further study, an important ally in therapies against poisoning by this metal. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-15 2015-11-11 2015-11-11 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
COSTA, Pauline da. ACTIVITY OF NTPDase, 5 - NUCLEOTIDASE, ACETYLCHOLINESTERASE AND PEROXIDATION LIPID LEVELS IN RATS EXPOSED TO CADMIUM AND TREATED WITH CURCUMIN. 2014. 91 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014. http://repositorio.ufsm.br/handle/1/11243 |
identifier_str_mv |
COSTA, Pauline da. ACTIVITY OF NTPDase, 5 - NUCLEOTIDASE, ACETYLCHOLINESTERASE AND PEROXIDATION LIPID LEVELS IN RATS EXPOSED TO CADMIUM AND TREATED WITH CURCUMIN. 2014. 91 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014. |
url |
http://repositorio.ufsm.br/handle/1/11243 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
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UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
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1805922119435943936 |