Patossistema Colletotrichum spp. x Carya illinoinensis, caracterização e controle

Detalhes bibliográficos
Autor(a) principal: Poletto, Tales
Data de Publicação: 2022
Tipo de documento: Tese
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
Texto Completo: http://repositorio.ufsm.br/handle/1/24175
Resumo: Pecan (Carya illinoinensis) is an important species cultivated commercially in several countries around the world. More than 100 years ago, North American cultivars were introduced in southern Brazil. Since this introduction, family farmers carry out the cultivation, propagation and selection of these cultivars, as well as plants of seminal origin, based on morpho-agronomic characteristics. This process gave rise to several other genotypes, regionally adapted and with productive potential. In this context, the recent increase in cultivated areas has increased the occurrence of diseases, such as anthracnose, urgently demanding efficient and safe phytosanitary control tools. The aim of this study was to characterize the Colletotrichum spp. x pecan, perform the morphometric, chemical and genetic characterization of pecan genotypes, as well as seek control tools for the disease, through sources of genetic resistance and the selection of biocontrol agents to Colletotrichum spp. For the characterization of the Colletotrichum spp. x pecan, morphological, molecular and pathogenic approaches were used to characterize Colletotrichum species, based on 68 isolates associated with symptomatic fruits, collected in the State of Rio Grande do Sul. The genetic diversity of pecan plants was characterized by morphometric and chemical methods and genetic-molecular analysis based on 60 pecan genotypes. Sensitivity tests were carried out in 18 genotypes, through the inoculation of fruits and leaflets with three species of Colletotrichum, under controlled conditions. Microsatellite molecular markers were used to estimate diversity and compare genotypes according to resistance to Colletotrichum spp. The biocontrol test was performed using six species of the antagonist Trichoderma spp. and five species of the pathogen Colletotrichum spp. The antagonist potential was evaluated by inhibiting the mycelial growth of the pathogens. Six species associated with pecan anthracnose were identified: C. nymphaeae, C. fioriniae, C. gloeosporioides, C. siamense, C. kahawae and C. karstii, which showed different aggressive responses in pecan fruits and leaflets. The genetic diversity of pecan genotypes was moderate to high, which could serve as a source of germplasm for crop improvement. In the sensitivity test, anthracnose manifested itself more severely in the fruits than in the leaflets. Genotypes 63, 47, 29 and 56 showed high resistance to anthracnose. There was no correlation between microsatellite markers and resistance to Colletotrichum spp., however moderate to high genetic diversity was observed. In the biocontrol test, the species T. virens and T. tomentosum showed the greatest potential antagonist to Colletotrichum spp. The results of this study contribute to the elucidation of the Colletotrichum spp. x pecan, as well as, provide subsidies for the development of future studies involving the improvement and control of anthracnose in the crop.
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spelling Patossistema Colletotrichum spp. x Carya illinoinensis, caracterização e controlePathosystem Colletotrichum spp. x Carya illinoinensis, characterization and controlePecanAntracnoseDiversidade genéticaAnthracnoseGenetical diversityCNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTALPecan (Carya illinoinensis) is an important species cultivated commercially in several countries around the world. More than 100 years ago, North American cultivars were introduced in southern Brazil. Since this introduction, family farmers carry out the cultivation, propagation and selection of these cultivars, as well as plants of seminal origin, based on morpho-agronomic characteristics. This process gave rise to several other genotypes, regionally adapted and with productive potential. In this context, the recent increase in cultivated areas has increased the occurrence of diseases, such as anthracnose, urgently demanding efficient and safe phytosanitary control tools. The aim of this study was to characterize the Colletotrichum spp. x pecan, perform the morphometric, chemical and genetic characterization of pecan genotypes, as well as seek control tools for the disease, through sources of genetic resistance and the selection of biocontrol agents to Colletotrichum spp. For the characterization of the Colletotrichum spp. x pecan, morphological, molecular and pathogenic approaches were used to characterize Colletotrichum species, based on 68 isolates associated with symptomatic fruits, collected in the State of Rio Grande do Sul. The genetic diversity of pecan plants was characterized by morphometric and chemical methods and genetic-molecular analysis based on 60 pecan genotypes. Sensitivity tests were carried out in 18 genotypes, through the inoculation of fruits and leaflets with three species of Colletotrichum, under controlled conditions. Microsatellite molecular markers were used to estimate diversity and compare genotypes according to resistance to Colletotrichum spp. The biocontrol test was performed using six species of the antagonist Trichoderma spp. and five species of the pathogen Colletotrichum spp. The antagonist potential was evaluated by inhibiting the mycelial growth of the pathogens. Six species associated with pecan anthracnose were identified: C. nymphaeae, C. fioriniae, C. gloeosporioides, C. siamense, C. kahawae and C. karstii, which showed different aggressive responses in pecan fruits and leaflets. The genetic diversity of pecan genotypes was moderate to high, which could serve as a source of germplasm for crop improvement. In the sensitivity test, anthracnose manifested itself more severely in the fruits than in the leaflets. Genotypes 63, 47, 29 and 56 showed high resistance to anthracnose. There was no correlation between microsatellite markers and resistance to Colletotrichum spp., however moderate to high genetic diversity was observed. In the biocontrol test, the species T. virens and T. tomentosum showed the greatest potential antagonist to Colletotrichum spp. The results of this study contribute to the elucidation of the Colletotrichum spp. x pecan, as well as, provide subsidies for the development of future studies involving the improvement and control of anthracnose in the crop.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESA nogueira-pecã (Carya illinoinensis) é uma importante espécie cultivada comercialmente em vários países do mundo. Há mais de 100 anos, cultivares norte americanas foram introduzidas no sul do Brasil. Desde essa introdução, os agricultores familiares realizam o cultivo, a propagação e a seleção dessas cultivares, assim como, plantas de origem seminal, baseando-se em características morfo-agronômicas. Desse processo, originaram-se vários outros genótipos, regionalmente adaptados e com potencial produtivo. Neste contexto, o recente aumento das áreas de cultivo incrementou a ocorrência de doenças, como a antracnose, demandando urgentemente por ferramentas de controle fitossanitário eficientes e seguras. O objetivo desse estudo foi caracterizar o patossistema Colletotrichum spp. x nogueira-pecã, realizar a caracterização morfométrica, química e genética de genótipos de nogueira-pecã, bem como, buscar ferramentas de controle para a doença, através de fontes de resistência genética e a seleção de agentes de biocontrole à Colletotrichum spp. Para a caracterização do patossistema Colletotrichum spp. x nogueira-pecã utilizou-se abordagens morfológicas, moleculares e patogênicas, para caracterizar espécies de Colletotrichum, com base em 68 isolados associados à frutos sintomáticos, coletados no Estado do Rio Grande do Sul. A diversidade genética das plantas de nogueira-pecã foi caracterizada por métodos morfométricos, químicos e análises genético-moleculares com base em 60 genótipos de nogueira-pecã. Os testes de sensibilidade foram realizados em 18 genótipos, por meio da inoculação de frutos e folíolos com três espécies de Colletotrichum, em condições controladas. Marcadores moleculares microssatélites foram utilizados para estimar a diversidade e comparar os genótipos de acordo com a resistência à Colletotrichum spp. O teste de biocontrole foi realizado utilizando seis espécies do antagonista Trichoderma spp. e cinco espécies do patógeno Colletotrichum spp. O potencial antagonista foi avaliado pela inibição do crescimento micelial dos patógenos. Foram identificadas seis espécies associadas à antracnose da nogueira-pecã: C. nymphaeae, C. fioriniae, C. gloeosporioides, C. siamense, C. kahawae e C. karstii, as quais apresentaram diferentes respostas de agressividades em frutos e folíolos de nogueira-pecã. A diversidade genética dos genótipos de nogueira-pecã foi moderada a alta, podendo servir como fonte de germoplasma para o melhoramento da cultura. No teste de sensibilidade, a antracnose se manifestou mais severamente nos frutos em relação aos folíolos. Os genótipos 63, 47, 29 e 56 apresentaram alta resistência a antracnose. Não houve correlação entre marcadores microssatélites e a resistência à Colletotrichum spp., porém foi observada moderada a alta diversidade genética. No teste de biocontrole, as espécies T. virens e T. tomentosum apresentaram maior potencial antagonista à Colletotrichum spp. Os resultados deste estudo contribuem para elucidação do patossistema Colletotrichum spp. x nogueira-pecã, bem como, trazem subsídios para o desenvolvimento de futuros estudos envolvendo melhoramento e controle da antracnose na cultura.Universidade Federal de Santa MariaBrasilRecursos Florestais e Engenharia FlorestalUFSMPrograma de Pós-Graduação em Engenharia FlorestalCentro de Ciências RuraisMuniz, Marlove Fatima Briãohttp://lattes.cnpq.br/3148312031889388Dantas, Adriana Cibele de MesquitaMaciel, Caciara GonzattoLudwig, JulianeStefenon, Valdir MarcosPoletto, Tales2022-04-25T14:29:52Z2022-04-25T14:29:52Z2022-02-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/24175porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-04-25T14:29:55Zoai:repositorio.ufsm.br:1/24175Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-04-25T14:29:55Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Patossistema Colletotrichum spp. x Carya illinoinensis, caracterização e controle
Pathosystem Colletotrichum spp. x Carya illinoinensis, characterization and controle
title Patossistema Colletotrichum spp. x Carya illinoinensis, caracterização e controle
spellingShingle Patossistema Colletotrichum spp. x Carya illinoinensis, caracterização e controle
Poletto, Tales
Pecan
Antracnose
Diversidade genética
Anthracnose
Genetical diversity
CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL
title_short Patossistema Colletotrichum spp. x Carya illinoinensis, caracterização e controle
title_full Patossistema Colletotrichum spp. x Carya illinoinensis, caracterização e controle
title_fullStr Patossistema Colletotrichum spp. x Carya illinoinensis, caracterização e controle
title_full_unstemmed Patossistema Colletotrichum spp. x Carya illinoinensis, caracterização e controle
title_sort Patossistema Colletotrichum spp. x Carya illinoinensis, caracterização e controle
author Poletto, Tales
author_facet Poletto, Tales
author_role author
dc.contributor.none.fl_str_mv Muniz, Marlove Fatima Brião
http://lattes.cnpq.br/3148312031889388
Dantas, Adriana Cibele de Mesquita
Maciel, Caciara Gonzatto
Ludwig, Juliane
Stefenon, Valdir Marcos
dc.contributor.author.fl_str_mv Poletto, Tales
dc.subject.por.fl_str_mv Pecan
Antracnose
Diversidade genética
Anthracnose
Genetical diversity
CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL
topic Pecan
Antracnose
Diversidade genética
Anthracnose
Genetical diversity
CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL
description Pecan (Carya illinoinensis) is an important species cultivated commercially in several countries around the world. More than 100 years ago, North American cultivars were introduced in southern Brazil. Since this introduction, family farmers carry out the cultivation, propagation and selection of these cultivars, as well as plants of seminal origin, based on morpho-agronomic characteristics. This process gave rise to several other genotypes, regionally adapted and with productive potential. In this context, the recent increase in cultivated areas has increased the occurrence of diseases, such as anthracnose, urgently demanding efficient and safe phytosanitary control tools. The aim of this study was to characterize the Colletotrichum spp. x pecan, perform the morphometric, chemical and genetic characterization of pecan genotypes, as well as seek control tools for the disease, through sources of genetic resistance and the selection of biocontrol agents to Colletotrichum spp. For the characterization of the Colletotrichum spp. x pecan, morphological, molecular and pathogenic approaches were used to characterize Colletotrichum species, based on 68 isolates associated with symptomatic fruits, collected in the State of Rio Grande do Sul. The genetic diversity of pecan plants was characterized by morphometric and chemical methods and genetic-molecular analysis based on 60 pecan genotypes. Sensitivity tests were carried out in 18 genotypes, through the inoculation of fruits and leaflets with three species of Colletotrichum, under controlled conditions. Microsatellite molecular markers were used to estimate diversity and compare genotypes according to resistance to Colletotrichum spp. The biocontrol test was performed using six species of the antagonist Trichoderma spp. and five species of the pathogen Colletotrichum spp. The antagonist potential was evaluated by inhibiting the mycelial growth of the pathogens. Six species associated with pecan anthracnose were identified: C. nymphaeae, C. fioriniae, C. gloeosporioides, C. siamense, C. kahawae and C. karstii, which showed different aggressive responses in pecan fruits and leaflets. The genetic diversity of pecan genotypes was moderate to high, which could serve as a source of germplasm for crop improvement. In the sensitivity test, anthracnose manifested itself more severely in the fruits than in the leaflets. Genotypes 63, 47, 29 and 56 showed high resistance to anthracnose. There was no correlation between microsatellite markers and resistance to Colletotrichum spp., however moderate to high genetic diversity was observed. In the biocontrol test, the species T. virens and T. tomentosum showed the greatest potential antagonist to Colletotrichum spp. The results of this study contribute to the elucidation of the Colletotrichum spp. x pecan, as well as, provide subsidies for the development of future studies involving the improvement and control of anthracnose in the crop.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-25T14:29:52Z
2022-04-25T14:29:52Z
2022-02-17
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
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dc.identifier.uri.fl_str_mv http://repositorio.ufsm.br/handle/1/24175
url http://repositorio.ufsm.br/handle/1/24175
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
Brasil
Recursos Florestais e Engenharia Florestal
UFSM
Programa de Pós-Graduação em Engenharia Florestal
Centro de Ciências Rurais
publisher.none.fl_str_mv Universidade Federal de Santa Maria
Brasil
Recursos Florestais e Engenharia Florestal
UFSM
Programa de Pós-Graduação em Engenharia Florestal
Centro de Ciências Rurais
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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