Adulteração de suplementos alimentares comercializados para a perda de peso
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/19137 |
Resumo: | Fat burners are dietary supplements used intentionally to promote weight loss, stimulating lipolysis or inhibiting lipogenesis. The great investment of the media, coupled with the search for a perfect body, has led to a massive consumption of these products. Open access to pharmacies, shops and even the e-commerces makes them easier to import, market and consume. Because of the huge growth of this market, new products are launched, often without due proof of efficacy and safety, which may lead to fraud or adulteration. Given this, there is a need for rapid analytical methods, sensitive and selective analysis of these products. Considering this, this work proposed the development of two analytical methods in HPLC DAD. The first method proposed the determination of caffeine in oily matrices in food supplements. High performance liquid chromatographic separation was achieved using a C18 reversed phase, a binary mixture of a 0.1% phosphoric and acetonitrile with a flow rate of 0.8 mL.min-1. UV detection was 220 nm. Water produced the best extraction efficiency in comparison with other solvents. Retention time observed for caffeine was 4.5 min. The second proposed the development of an analytical method for the determination of 11 adulterants (stimulants, anorectic, antidepressants, antiepileptic, diuretics and laxatives). The chromatographic analysis were conduced at temperature de 35ºC±1ºC using a reversed- phase C18 column, mobile phase gradient was a composed of ammonium acetate 100 mmol.L-1 (pH 5.2), acetonitrile and tetrahydrofuran, flux rate: 1.0 mL.min-1 and UV detection was 224, 250 e 270 nm. A 13 minutes chromatographic run was able to separate all adulterants. anfepramone, caffeine, lamotrigine, bupropion, phenolphtalein, fluoxetine, sertraline, furosemide, bisacodyl, hydroclorotiazide and sibutramine were simultaneously analysed in all samples (n=30). One sample was adulterated with the diuretic furosemide. Both methods were quick, sensible, selective and reproducible in the proposed linear range. The caffeine method to be analytical tool applicable to the oily capsules analysis in time release dietary supplements and the development simultaneous method to be analytical tool applicable to the screening of adulteration of supplements used as fat burners. |
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Adulteração de suplementos alimentares comercializados para a perda de pesoSuplementos alimentaresAdulterantesCromatografia líquidaHPLC DADDietary supplementsAdulterationLiquid chromatographyCNPQ::CIENCIAS DA SAUDE::FARMACIAFat burners are dietary supplements used intentionally to promote weight loss, stimulating lipolysis or inhibiting lipogenesis. The great investment of the media, coupled with the search for a perfect body, has led to a massive consumption of these products. Open access to pharmacies, shops and even the e-commerces makes them easier to import, market and consume. Because of the huge growth of this market, new products are launched, often without due proof of efficacy and safety, which may lead to fraud or adulteration. Given this, there is a need for rapid analytical methods, sensitive and selective analysis of these products. Considering this, this work proposed the development of two analytical methods in HPLC DAD. The first method proposed the determination of caffeine in oily matrices in food supplements. High performance liquid chromatographic separation was achieved using a C18 reversed phase, a binary mixture of a 0.1% phosphoric and acetonitrile with a flow rate of 0.8 mL.min-1. UV detection was 220 nm. Water produced the best extraction efficiency in comparison with other solvents. Retention time observed for caffeine was 4.5 min. The second proposed the development of an analytical method for the determination of 11 adulterants (stimulants, anorectic, antidepressants, antiepileptic, diuretics and laxatives). The chromatographic analysis were conduced at temperature de 35ºC±1ºC using a reversed- phase C18 column, mobile phase gradient was a composed of ammonium acetate 100 mmol.L-1 (pH 5.2), acetonitrile and tetrahydrofuran, flux rate: 1.0 mL.min-1 and UV detection was 224, 250 e 270 nm. A 13 minutes chromatographic run was able to separate all adulterants. anfepramone, caffeine, lamotrigine, bupropion, phenolphtalein, fluoxetine, sertraline, furosemide, bisacodyl, hydroclorotiazide and sibutramine were simultaneously analysed in all samples (n=30). One sample was adulterated with the diuretic furosemide. Both methods were quick, sensible, selective and reproducible in the proposed linear range. The caffeine method to be analytical tool applicable to the oily capsules analysis in time release dietary supplements and the development simultaneous method to be analytical tool applicable to the screening of adulteration of supplements used as fat burners.Suplementos alimentares, conhecidos como “queimadores de gordura” são usados intencionalmente para promover a perda de peso, estimular a lipólise ou inibir a lipogênese. O grande investimento da mídia, aliada à busca por um corpo perfeito, tem levado a um consumo massivo destes produtos. O livre acesso em farmácias, lojas e até mesmo pela internet os torna alvo de fácil importação, comercialização e consumo. Em consequência ao grande crescimento deste mercado, novos produtos são lançados, muitas vezes sem a devida comprovação da eficácia e segurança o que pode levar a ocorrência de fraudes ou adulterações. Diante disso, verifica-se a necessidade de métodos analíticos rápidos, sensíveis e seletivas análises destes produtos. Assim, este trabalho propôs o desenvolvimento de dois métodos analíticos em HPLC DAD e análise de suplementos alimentares comercializados no mercado virtual brasileiro e lojas físicas. O primeiro método propôs a determinação de cafeína em matrizes oleosas em amostras de suplementos alimentares. A análise cromatográfica da cafeína foi conduzida em uma coluna de fase reversa C18, fase móvel: ácido acético 0,1% e acetonitrila, fluxo: 0,8 mL.min-1 e detecção UV a 220 nm. A água produziu a melhor eficiência de extração da cafeína nas amostras em comparação com outros solventes. O tempo de retenção da cafeína foi de 4,5 minutos. O segundo método propôs o desenvolvimento de um método analítico para a determinação de 11 adulterantes (estimulantes, anorexígenos, antidepressivos, antiepiléptico, diuréticos e laxantes). A análise cromatográfica dos 11 compostos foi conduzida à temperatura de 35ºC±1ºC usando uma coluna de fase reversa C18, fase móvel: acetato de amônio 100 mmol.L-1 (pH 5,2), acetonitrila e tetrahidrofurano, fluxo: 1,0 mL.min-1 e detecção UV a 224, 250 e 270 nm. O tempo de 13 minutos foi efetivo para a separação de todos os adulterantes. Anfepramona, cafeína, lamotrigina, bupropiona, fenolftaleína, fluoxetina, sertralina, furosemida, bisacodil, hidroclorotiazida e sibutramina foram determinados simultaneamente em todas as amostras (n=30). Uma amostra estava adulterada pelo diurético furosemida. Ambos os métodos foram rápidos, sensíveis e seletivos na faixa linear proposta. O método da cafeína pode ser aplicado a análise de encapsulados oleosos de liberação controlada em suplementos alimentares e o método simultâneo pode ser aplicado a triagem de suplementos alimentares comercializados para a perda de peso.Universidade Federal de Santa MariaBrasilFarmacologiaUFSMPrograma de Pós-Graduação em Ciências FarmacêuticasCentro de Ciências da SaúdeSilva, Carine Vianahttp://lattes.cnpq.br/2004872342535591Cardoso, Carmem Dickowhttp://lattes.cnpq.br/3886927196242959Nascimento, Paulo Cícero dohttp://lattes.cnpq.br/7151513617218161Mortari, Sergio Robertohttp://lattes.cnpq.br/7784609477475171Kunz, Simone Noremberghttp://lattes.cnpq.br/0979416016892842Ribeiro, Sandra Maria2019-12-10T19:42:20Z2019-12-10T19:42:20Z2019-08-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/19137porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2019-12-11T06:02:27Zoai:repositorio.ufsm.br:1/19137Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2019-12-11T06:02:27Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Adulteração de suplementos alimentares comercializados para a perda de peso |
title |
Adulteração de suplementos alimentares comercializados para a perda de peso |
spellingShingle |
Adulteração de suplementos alimentares comercializados para a perda de peso Ribeiro, Sandra Maria Suplementos alimentares Adulterantes Cromatografia líquida HPLC DAD Dietary supplements Adulteration Liquid chromatography CNPQ::CIENCIAS DA SAUDE::FARMACIA |
title_short |
Adulteração de suplementos alimentares comercializados para a perda de peso |
title_full |
Adulteração de suplementos alimentares comercializados para a perda de peso |
title_fullStr |
Adulteração de suplementos alimentares comercializados para a perda de peso |
title_full_unstemmed |
Adulteração de suplementos alimentares comercializados para a perda de peso |
title_sort |
Adulteração de suplementos alimentares comercializados para a perda de peso |
author |
Ribeiro, Sandra Maria |
author_facet |
Ribeiro, Sandra Maria |
author_role |
author |
dc.contributor.none.fl_str_mv |
Silva, Carine Viana http://lattes.cnpq.br/2004872342535591 Cardoso, Carmem Dickow http://lattes.cnpq.br/3886927196242959 Nascimento, Paulo Cícero do http://lattes.cnpq.br/7151513617218161 Mortari, Sergio Roberto http://lattes.cnpq.br/7784609477475171 Kunz, Simone Noremberg http://lattes.cnpq.br/0979416016892842 |
dc.contributor.author.fl_str_mv |
Ribeiro, Sandra Maria |
dc.subject.por.fl_str_mv |
Suplementos alimentares Adulterantes Cromatografia líquida HPLC DAD Dietary supplements Adulteration Liquid chromatography CNPQ::CIENCIAS DA SAUDE::FARMACIA |
topic |
Suplementos alimentares Adulterantes Cromatografia líquida HPLC DAD Dietary supplements Adulteration Liquid chromatography CNPQ::CIENCIAS DA SAUDE::FARMACIA |
description |
Fat burners are dietary supplements used intentionally to promote weight loss, stimulating lipolysis or inhibiting lipogenesis. The great investment of the media, coupled with the search for a perfect body, has led to a massive consumption of these products. Open access to pharmacies, shops and even the e-commerces makes them easier to import, market and consume. Because of the huge growth of this market, new products are launched, often without due proof of efficacy and safety, which may lead to fraud or adulteration. Given this, there is a need for rapid analytical methods, sensitive and selective analysis of these products. Considering this, this work proposed the development of two analytical methods in HPLC DAD. The first method proposed the determination of caffeine in oily matrices in food supplements. High performance liquid chromatographic separation was achieved using a C18 reversed phase, a binary mixture of a 0.1% phosphoric and acetonitrile with a flow rate of 0.8 mL.min-1. UV detection was 220 nm. Water produced the best extraction efficiency in comparison with other solvents. Retention time observed for caffeine was 4.5 min. The second proposed the development of an analytical method for the determination of 11 adulterants (stimulants, anorectic, antidepressants, antiepileptic, diuretics and laxatives). The chromatographic analysis were conduced at temperature de 35ºC±1ºC using a reversed- phase C18 column, mobile phase gradient was a composed of ammonium acetate 100 mmol.L-1 (pH 5.2), acetonitrile and tetrahydrofuran, flux rate: 1.0 mL.min-1 and UV detection was 224, 250 e 270 nm. A 13 minutes chromatographic run was able to separate all adulterants. anfepramone, caffeine, lamotrigine, bupropion, phenolphtalein, fluoxetine, sertraline, furosemide, bisacodyl, hydroclorotiazide and sibutramine were simultaneously analysed in all samples (n=30). One sample was adulterated with the diuretic furosemide. Both methods were quick, sensible, selective and reproducible in the proposed linear range. The caffeine method to be analytical tool applicable to the oily capsules analysis in time release dietary supplements and the development simultaneous method to be analytical tool applicable to the screening of adulteration of supplements used as fat burners. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-10T19:42:20Z 2019-12-10T19:42:20Z 2019-08-22 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/19137 |
url |
http://repositorio.ufsm.br/handle/1/19137 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Farmacologia UFSM Programa de Pós-Graduação em Ciências Farmacêuticas Centro de Ciências da Saúde |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Farmacologia UFSM Programa de Pós-Graduação em Ciências Farmacêuticas Centro de Ciências da Saúde |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922141474914304 |