Patogenia experimental da infecção genital aguda e latente pelo herpesvírus bovino tipo 1.2 em bezerras
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/10010 |
Resumo: | This study aimed at reproducting and characterizing the virological and clinico-pathological aspects of acute and latent genital infection by bovine herpesvirus type 1.2 (BoHV-1.2) in heifers. Four heifers were inoculated intravaginally with a Brazilian BoHV-1.2 isolate (SV-56/90) recovered from an outbreak of balanoposthitis. Virus inoculation (108.1TCID50/animal) resulted in efficient virus replication in the genital mucosa and the development of moderate to severe vulvovaginitis. The inoculated heifers shed virus in genital secretions in titers up to 107.3TCID50/mL until day 10 post infection (pi). Hyperemia, edema of vulvar and vestibular mucosa, vesicles, pustules and scabs were observed between days 1 and 10 pi. The vesicles and pustules increased in size and eventually coalesced and became covered with a mucopurulent and fibrinous exsudate. These signs increased in severity up to days 5 8 pi and progressively subsided thereafter. Dexamethasone administration at day 55 pi resulted in virus shedding in vaginal secretions of all heifers for up to 10 days. Virus reactivation was accompanied by genital signs resembling those observed during acute infection, yet less severe. Examination of the lumbar sacral ganglia and lymph nodes by PCR at day 36 postreactivation revealed the presence of latent viral DNA in the pudendal (4/4), genito-femoral, sciatic and rectal caudal (3/4) and obturator nerve ganglia (1/4); and in the sacral (3/4), deep inguinal, pre femoral, supramammary (2/4) and medial iliac lymph nodes (1/4). These results demonstrate that BoHV-1.2 DNA persists in sacral ganglia and regional lymph nodes during latency, and help in understanding the pathogenesis of acute and latent genital infection by BoHV-1.2. |
id |
UFSM_bf22c6dac3945040e9d56ab014223ef6 |
---|---|
oai_identifier_str |
oai:repositorio.ufsm.br:1/10010 |
network_acronym_str |
UFSM |
network_name_str |
Manancial - Repositório Digital da UFSM |
repository_id_str |
|
spelling |
Patogenia experimental da infecção genital aguda e latente pelo herpesvírus bovino tipo 1.2 em bezerrasExperimental pathogenesis of acute and latent genital infection by bovine herpesvirus type 1.2 in heifersHerpesvírus bovinoBoHV-1.2VulvovaginiteInfecção genitalReativaçãoBovine herpesvirusBoHV-1.2VulvovaginitisGenital infectionReactivationCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAThis study aimed at reproducting and characterizing the virological and clinico-pathological aspects of acute and latent genital infection by bovine herpesvirus type 1.2 (BoHV-1.2) in heifers. Four heifers were inoculated intravaginally with a Brazilian BoHV-1.2 isolate (SV-56/90) recovered from an outbreak of balanoposthitis. Virus inoculation (108.1TCID50/animal) resulted in efficient virus replication in the genital mucosa and the development of moderate to severe vulvovaginitis. The inoculated heifers shed virus in genital secretions in titers up to 107.3TCID50/mL until day 10 post infection (pi). Hyperemia, edema of vulvar and vestibular mucosa, vesicles, pustules and scabs were observed between days 1 and 10 pi. The vesicles and pustules increased in size and eventually coalesced and became covered with a mucopurulent and fibrinous exsudate. These signs increased in severity up to days 5 8 pi and progressively subsided thereafter. Dexamethasone administration at day 55 pi resulted in virus shedding in vaginal secretions of all heifers for up to 10 days. Virus reactivation was accompanied by genital signs resembling those observed during acute infection, yet less severe. Examination of the lumbar sacral ganglia and lymph nodes by PCR at day 36 postreactivation revealed the presence of latent viral DNA in the pudendal (4/4), genito-femoral, sciatic and rectal caudal (3/4) and obturator nerve ganglia (1/4); and in the sacral (3/4), deep inguinal, pre femoral, supramammary (2/4) and medial iliac lymph nodes (1/4). These results demonstrate that BoHV-1.2 DNA persists in sacral ganglia and regional lymph nodes during latency, and help in understanding the pathogenesis of acute and latent genital infection by BoHV-1.2.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorO presente estudo teve como objetivos reproduzir e caracterizar os aspectos virológicos e clinico-patológicos da infecção genital aguda e latente pelo herpesvírus bovino tipo 1.2 (BoHV-1.2) em bezerras. Quatro bezerras foram inoculadas no trato genital com um isolado brasileiro de BoHV-1.2 (SV-56/90) oriundo de um surto de balanopostite. A inoculação do vírus (108,1TCID50/animal) resultou em replicação viral eficiente na mucosa genital e no desenvolvimento de vulvovaginite moderada a severa. As bezerras inoculadas excretaram vírus nas secreções genitais em títulos de até 107,3TCID50/mL por até 10 dias pós-inoculação (pi). Hiperemia, edema das mucosas vulvar e vestibular, vesículas, pústulas e crostas foram observados entre os dias 1° e 10° dia pi. As vesículas e pústulas aumentaram de diâmetro, eventualmente coalesceram e tornaram-se recobertas com um exsudato mucopurulento e fibrinoso. Os sinais aumentaram em severidade do 5° ao 8° dia pi e regrediram a partir de então. Administração de dexametasona dia 55 pi resultou em excreção viral nas secreções vaginais por até 10 dias. A reativação viral foi acompanhada de sinais genitais semelhantes aos observados durante a infecção aguda, porém com menor severidade. A análise dos gânglios lombo-sacral e linfonodos regionais por PCR no dia 36 pós-reativação demonstrou a presença de DNA viral latente nos gânglios pudendo (4/4), genito-femural, ciático, retalcaudal (3/4) e obturador (1/4); e nos linfonodos sacral (3/4), inguinal profundo, pré-femural, supramamário (2/4) e ilíaco medial (1/4). Esses resultados demonstram que o DNA do BoHV-1.2 persiste nos gânglios sacrais e em linfonodos regionais durante a latência, e contribuem para o conhecimento da patogenia da infecção genital aguda e latente pelo BoHV-1.2.Universidade Federal de Santa MariaBRMedicina VeterináriaUFSMPrograma de Pós-Graduação em Medicina VeterináriaWeiblen, Rudihttp://lattes.cnpq.br/7946350215388090Lovato, Luciane Teresinhahttp://lattes.cnpq.br/6205014536180094Caron, Luizinhohttp://lattes.cnpq.br/9334336945441958Henzel, Andréia2017-05-292017-05-292008-02-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfHENZEL, Andréia. Experimental pathogenesis of acute and latent genital infection by bovine herpesvirus type 1.2 in heifers. 2008. 43 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2008.http://repositorio.ufsm.br/handle/1/10010porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-09-12T16:42:50Zoai:repositorio.ufsm.br:1/10010Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-09-12T16:42:50Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Patogenia experimental da infecção genital aguda e latente pelo herpesvírus bovino tipo 1.2 em bezerras Experimental pathogenesis of acute and latent genital infection by bovine herpesvirus type 1.2 in heifers |
title |
Patogenia experimental da infecção genital aguda e latente pelo herpesvírus bovino tipo 1.2 em bezerras |
spellingShingle |
Patogenia experimental da infecção genital aguda e latente pelo herpesvírus bovino tipo 1.2 em bezerras Henzel, Andréia Herpesvírus bovino BoHV-1.2 Vulvovaginite Infecção genital Reativação Bovine herpesvirus BoHV-1.2 Vulvovaginitis Genital infection Reactivation CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Patogenia experimental da infecção genital aguda e latente pelo herpesvírus bovino tipo 1.2 em bezerras |
title_full |
Patogenia experimental da infecção genital aguda e latente pelo herpesvírus bovino tipo 1.2 em bezerras |
title_fullStr |
Patogenia experimental da infecção genital aguda e latente pelo herpesvírus bovino tipo 1.2 em bezerras |
title_full_unstemmed |
Patogenia experimental da infecção genital aguda e latente pelo herpesvírus bovino tipo 1.2 em bezerras |
title_sort |
Patogenia experimental da infecção genital aguda e latente pelo herpesvírus bovino tipo 1.2 em bezerras |
author |
Henzel, Andréia |
author_facet |
Henzel, Andréia |
author_role |
author |
dc.contributor.none.fl_str_mv |
Weiblen, Rudi http://lattes.cnpq.br/7946350215388090 Lovato, Luciane Teresinha http://lattes.cnpq.br/6205014536180094 Caron, Luizinho http://lattes.cnpq.br/9334336945441958 |
dc.contributor.author.fl_str_mv |
Henzel, Andréia |
dc.subject.por.fl_str_mv |
Herpesvírus bovino BoHV-1.2 Vulvovaginite Infecção genital Reativação Bovine herpesvirus BoHV-1.2 Vulvovaginitis Genital infection Reactivation CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
topic |
Herpesvírus bovino BoHV-1.2 Vulvovaginite Infecção genital Reativação Bovine herpesvirus BoHV-1.2 Vulvovaginitis Genital infection Reactivation CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
This study aimed at reproducting and characterizing the virological and clinico-pathological aspects of acute and latent genital infection by bovine herpesvirus type 1.2 (BoHV-1.2) in heifers. Four heifers were inoculated intravaginally with a Brazilian BoHV-1.2 isolate (SV-56/90) recovered from an outbreak of balanoposthitis. Virus inoculation (108.1TCID50/animal) resulted in efficient virus replication in the genital mucosa and the development of moderate to severe vulvovaginitis. The inoculated heifers shed virus in genital secretions in titers up to 107.3TCID50/mL until day 10 post infection (pi). Hyperemia, edema of vulvar and vestibular mucosa, vesicles, pustules and scabs were observed between days 1 and 10 pi. The vesicles and pustules increased in size and eventually coalesced and became covered with a mucopurulent and fibrinous exsudate. These signs increased in severity up to days 5 8 pi and progressively subsided thereafter. Dexamethasone administration at day 55 pi resulted in virus shedding in vaginal secretions of all heifers for up to 10 days. Virus reactivation was accompanied by genital signs resembling those observed during acute infection, yet less severe. Examination of the lumbar sacral ganglia and lymph nodes by PCR at day 36 postreactivation revealed the presence of latent viral DNA in the pudendal (4/4), genito-femoral, sciatic and rectal caudal (3/4) and obturator nerve ganglia (1/4); and in the sacral (3/4), deep inguinal, pre femoral, supramammary (2/4) and medial iliac lymph nodes (1/4). These results demonstrate that BoHV-1.2 DNA persists in sacral ganglia and regional lymph nodes during latency, and help in understanding the pathogenesis of acute and latent genital infection by BoHV-1.2. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-02-26 2017-05-29 2017-05-29 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
HENZEL, Andréia. Experimental pathogenesis of acute and latent genital infection by bovine herpesvirus type 1.2 in heifers. 2008. 43 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2008. http://repositorio.ufsm.br/handle/1/10010 |
identifier_str_mv |
HENZEL, Andréia. Experimental pathogenesis of acute and latent genital infection by bovine herpesvirus type 1.2 in heifers. 2008. 43 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2008. |
url |
http://repositorio.ufsm.br/handle/1/10010 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Medicina Veterinária UFSM Programa de Pós-Graduação em Medicina Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Medicina Veterinária UFSM Programa de Pós-Graduação em Medicina Veterinária |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922166035709952 |